BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	83.5%	721.6 / 151.3	284	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (19/28); new base C (115/122); total (134/150)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTT  >  NC_000913/3980197‑3980381

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                 >  1:9855882/1‑93 (MQ=255)

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                 >  2:1470875/1‑93 (MQ=255)

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                 >  2:6367754/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc                                                                                             >  1:6429906/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc                                                                                             >  1:7571904/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc                                                                                             >  2:7072915/1‑93 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑c                                                                                           >  2:1010271/1‑93 (MQ=255)

     ttaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑‑T‑‑cc‑ccc                                                                                         >  2:9574456/1‑93 (MQ=255)

      taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccc                                                                                        <  1:764432/93‑1 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGGCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      <  2:1715815/93‑1 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccgc                                                                                      <  2:2988272/93‑3 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      <  2:4080050/93‑1 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      >  1:1283228/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      >  1:1204090/1‑93 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccct                                                                                    <  1:2268264/93‑3 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccct                                                                                    <  2:7502/93‑3 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGCGGGGTCAAG‑‑T‑‑gc‑cccccccc                                                                                    >  2:3671242/1‑91 (MQ=14)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccctc                                                                                   >  2:9529981/1‑90 (MQ=255)

            ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccctcg                                                                                  >  1:5097185/1‑89 (MQ=255)

            ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccctcg                                                                                  >  2:8637209/1‑89 (MQ=255)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGcaccac                                                                             <  1:1095522/93‑1 (MQ=16)

                   aaTGCGAAGGTGGCTGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCCCCACGa                                                                           <  1:9090834/93‑1 (MQ=255)

                    aggCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGa                                                                           <  1:7774696/91‑1 (MQ=255)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGa                                                                           >  1:1462828/1‑93 (MQ=255)

                      gCGAAGGTGGCGGAATTGGAAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACt                                                                         <  2:8960542/93‑1 (MQ=255)

                       cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACtt                                                                        <  1:3818634/93‑1 (MQ=255)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACttt                                                                       <  2:4652069/93‑1 (MQ=255)

                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGC‑C‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGa                                                                  <  1:8155174/93‑1 (MQ=255)

                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGa                                                                  <  2:9328797/93‑1 (MQ=255)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCGCACCACGACTTTAAAGaa                                                                 <  2:5305879/93‑1 (MQ=14)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAAAAt                                                                >  1:4202800/1‑93 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAAtt                                                               <  2:5615636/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGa                                                             >  1:8296014/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGa                                                             >  1:4443447/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGaa                                                            >  2:2784278/1‑93 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTTTTAGTGCCCTTACGGACGTGGGGGTTCAAGC‑C‑‑CC‑CCCCCCTCGCACCCCGACTTTAAAGAATTGAAc                                                           <  2:4677293/93‑1 (MQ=17)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAAc                                                           <  2:347433/93‑1 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACt                                                          >  1:2890085/1‑93 (MQ=255)

                                     ttGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGTGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACt                                                          >  2:2415002/1‑93 (MQ=255)

                                      tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACt                                                          <  1:6687468/93‑1 (MQ=17)

                                       ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGCTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaa                                                        >  1:5272634/1‑93 (MQ=255)

                                          aGACGCGCTCGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                     <  1:5688778/93‑1 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                     >  1:7390251/1‑93 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                     <  1:9424218/93‑1 (MQ=255)

                                           gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                                    >  1:6982472/1‑93 (MQ=255)

                                            aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAtt                                                   >  1:9303469/1‑93 (MQ=255)

                                            aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCCCGCACCACGACTTTTAAGAATTGAACTAAAAAtt                                                   >  1:5980101/1‑93 (MQ=18)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTCGGGGGTCAAG‑‑TC‑CC‑CCCCCCTCGCCCCACGACCTTAAAGAATTTAACTAAAAATTTaa                                                >  1:4885400/1‑93 (MQ=18)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGC‑C‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                              <  2:8266652/93‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CCACCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                              >  1:3150402/1‑93 (MQ=255)

                                                 cTAGCTTCAGGGGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CCACCCCCCTCGCAACACGACCTTAAAGAAATGAACTAAAAATTCaaaa                                              >  1:5765502/1‑93 (MQ=18)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                             <  2:9831619/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                             <  2:7319810/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                             >  2:5147530/1‑93 (MQ=255)

                                                   aGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                            <  1:3080410/93‑1 (MQ=255)

                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                           <  1:9608465/93‑1 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                         >  2:658858/1‑93 (MQ=255)

                                                       tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                        <  1:6791214/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGTGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  2:9133312/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  2:5845432/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  1:8727229/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  2:1897926/93‑1 (MQ=255)

                                                         aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                      <  2:1576138/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                     <  2:2672384/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                     >  1:3385240/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                     >  2:4568071/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑G‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                    <  2:7968334/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                    <  1:3936342/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                    <  1:2037292/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                   >  1:5403870/1‑93 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                   <  2:1220292/93‑1 (MQ=255)

                                                             gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                  <  2:3884534/93‑1 (MQ=255)

                                                               tAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                <  1:2686068/93‑1 (MQ=255)

                                                               tAGTGTCCTTACGGACGTGGGGGGTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                <  1:3334615/93‑1 (MQ=255)

                                                                aGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  2:5426187/93‑1 (MQ=255)

                                                                aGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  1:7975298/93‑1 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  2:4352917/93‑1 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  1:3059367/93‑1 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                             <  1:6927142/93‑1 (MQ=255)

                                                                    tCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                           <  2:1769570/93‑1 (MQ=255)

                                                                    gccTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                          <  1:2832333/92‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCCCACCCCGACTTTTAAAAATTTAAATAAAAAATTAAAAAACAGTATTTTGGCGAgga                           >  1:8968266/1‑91 (MQ=12)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                          <  1:1342550/93‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                          <  1:9910923/93‑1 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                         <  1:8802786/93‑1 (MQ=255)

                                                                       ttACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                        <  1:864918/93‑1 (MQ=255)

                                                                        tACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                       >  1:10022965/1‑93 (MQ=255)

                                                                        tACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcga                       >  1:2462480/1‑92 (MQ=255)

                                                                          cGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCa                      <  2:3188170/93‑1 (MQ=39)

                                                                            gACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCCGCt                   <  2:8453762/93‑1 (MQ=255)

                                                                             gcGTGGGGGTTCAAGCCC‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt                   <  2:6123861/92‑1 (MQ=255)

                                                                                 gggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa              <  1:1840608/93‑1 (MQ=255)

                                                                                 gggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa              <  1:6546340/93‑1 (MQ=255)

                                                                                 gggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa              <  1:169463/93‑1 (MQ=255)

                                                                                  ggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg             >  1:4760695/1‑93 (MQ=255)

                                                                                  ggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg             <  2:6946753/93‑1 (MQ=255)

                                                                                    ggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcg           >  2:6761316/1‑93 (MQ=255)

                                                                                    ggTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcgc          >  1:6112544/1‑93 (MQ=255)

                                                                                      ttCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGCAGCGCAGCTTGGTAGCGCa         >  1:6219563/1‑93 (MQ=255)

                                                                                           g‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGt   >  2:8177696/1‑93 (MQ=255)

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTT  >  NC_000913/3980197‑3980381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`