BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,984	+C	coding (1250/1299 nt)	trkH →	TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,983	1	.	C	99.8%	1400.3 / ‑4.5	435	coding (1249/1299 nt)	trkH	TrkH
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (264/170); total (264/171)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 477 total aligned reads displayed.

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA  >  NC_000913/4031891‑4032070

 tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                         <  2:2356657/93‑1 (MQ=255)

 tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                         >  1:3627048/1‑93 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                       >  2:3934820/1‑91 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                       >  2:6762247/1‑91 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                       <  1:1967055/93‑3 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                 >  1:2625900/1‑93 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                 >  2:4914329/1‑93 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                 >  2:6287969/1‑93 (MQ=255)

          gCCGTAACGCATGCTTGAACCTGTGTGGGGATTTTTCTCCTCCTTTCAATTGGTGTTTATTGTCAGTATGCTGGCCATTATCGCC‑ACGGGCGt                                                                                <  2:3228112/93‑1 (MQ=255)

          gCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGt                                                                                <  2:6149247/93‑1 (MQ=255)

           ccGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTg                                                                               >  1:4508122/1‑93 (MQ=255)

            cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCgggg                                                                              >  2:4665008/1‑93 (MQ=255)

             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             >  2:9869702/1‑93 (MQ=255)

             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             >  1:9750702/1‑93 (MQ=255)

             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             <  1:5588835/93‑1 (MQ=255)

              gAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAt                                                                            >  1:8818223/1‑93 (MQ=255)

                aCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGa                                                                          >  2:4349621/1‑93 (MQ=255)

                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACtt                                                                       <  1:779745/93‑1 (MQ=255)

                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  1:7252523/1‑93 (MQ=255)

                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  1:8650363/1‑93 (MQ=255)

                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      <  1:7606811/93‑1 (MQ=255)

                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTc                                                                    <  1:4823398/93‑1 (MQ=255)

                       cTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCt                                                                   >  2:5225800/1‑93 (MQ=255)

                           aaGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCt                                                               <  2:3765523/93‑1 (MQ=255)

                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCtt                                                              <  2:1361577/93‑1 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                             >  1:7004174/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                             <  2:420234/93‑1 (MQ=255)

                               cGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGc                                                           <  1:2981310/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          >  1:5326494/1‑93 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          <  2:930021/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          >  2:7378673/1‑93 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTCCCTTTGCg                                                          >  2:504477/1‑93 (MQ=255)

                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATTACTTTTCTGCCTTTGCGt                                                         >  2:7467775/1‑93 (MQ=255)

                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                         <  2:3425327/93‑1 (MQ=255)

                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                         >  1:10016115/1‑93 (MQ=255)

                                   tGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCg                                                       >  2:5540864/1‑93 (MQ=255)

                                     gggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgt                                                     <  2:8731167/93‑1 (MQ=255)

                                      ggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtt                                                    >  1:4400448/1‑93 (MQ=255)

                                       gATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttg                                                   >  1:2364531/1‑93 (MQ=255)

                                       gATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttg                                                   <  1:312009/93‑1 (MQ=255)

                                        aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtttt                                                  <  2:8397274/93‑3 (MQ=255)

                                        aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgt                                                  <  2:6433304/93‑1 (MQ=255)

                                         tttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgtt                                                 >  1:7365908/1‑93 (MQ=255)

                                          ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                                >  1:4204285/1‑93 (MQ=255)

                                           tttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGc                                               >  1:8156715/1‑93 (MQ=255)

                                              ctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAc                                            <  1:6272622/93‑1 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                           >  1:4994507/1‑93 (MQ=255)

                                                  gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                        <  1:1523473/93‑1 (MQ=255)

                                                   ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       <  1:6614852/93‑1 (MQ=255)

                                                   ccTATGCATGGGTGTTTATTGTCAGTATGCTGGCGAGTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       <  1:6067645/93‑1 (MQ=255)

                                                    ctttGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CTACGTGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTCCGACATTGaa                                      <  2:8538798/90‑1 (MQ=255)

                                                    cTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaa                                      <  2:213830/93‑1 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                     >  2:7269039/1‑93 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                    >  1:1408258/1‑93 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  2:7725766/1‑93 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  2:3540657/1‑93 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  1:8770492/1‑93 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                  <  1:6466263/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                <  2:2510103/93‑1 (MQ=255)

                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                               <  1:8401854/93‑1 (MQ=255)

                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                               >  1:4340921/1‑93 (MQ=255)

                                                            tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                              >  1:7953636/1‑93 (MQ=255)

                                                             ggTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                             >  1:9559499/1‑93 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  1:2458652/1‑93 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  1:1737517/1‑93 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  2:7134814/1‑93 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTAGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  2:1400445/1‑93 (MQ=255)

                                                                 tttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCa                         <  2:916661/93‑1 (MQ=255)

                                                                  ttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAg                        >  2:8005210/1‑93 (MQ=255)

                                                                   tATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                       >  2:8136887/1‑93 (MQ=255)

                                                                   tATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                       >  2:9475013/1‑93 (MQ=255)

                                                                    aTTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAggg                      >  2:8246324/1‑93 (MQ=255)

                                                                     ttGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGc                     <  2:6612569/93‑1 (MQ=255)

                                                                     ttGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGc                     >  1:1349684/1‑93 (MQ=255)

                                                                      tGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCt                    >  1:9333753/1‑93 (MQ=255)

                                                                       gTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                   >  2:6918478/1‑93 (MQ=255)

                                                                       gTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                   <  1:5085370/93‑1 (MQ=255)

                                                                        tCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTg                  >  2:2657822/1‑93 (MQ=255)

                                                                        tCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTg                  <  2:9006935/93‑1 (MQ=255)

                                                                         cAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTgg                 >  2:2775789/1‑93 (MQ=255)

                                                                          aGTATGCTGGCGATTATCGCC‑ACGGGAGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGc                >  2:1116270/1‑93 (MQ=255)

                                                                           gTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCg               <  2:2904752/93‑1 (MQ=255)

                                                                            tATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGt              <  2:9674172/93‑1 (MQ=255)

                                                                            gaTGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGt              <  1:3943858/92‑1 (MQ=255)

                                                                             aTGCTGGCGATTATCGCC‑ACGGGCGTGGCTGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGTGGACAGGGCTTGGCGTg             >  1:9514050/1‑93 (MQ=255)

                                                                             aTGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTg             <  1:8571785/93‑1 (MQ=255)

                                                                             aTGCTAGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTg             >  2:4479432/1‑93 (MQ=255)

                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            >  2:3047151/1‑93 (MQ=255)

                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            >  1:3378671/1‑93 (MQ=255)

                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            >  1:9972515/1‑93 (MQ=255)

                                                                                cTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGtt          <  1:7026786/93‑1 (MQ=255)

                                                                                cTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGtt          >  1:9128671/1‑93 (MQ=255)

                                                                                 tGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTg         <  1:7495311/93‑1 (MQ=255)

                                                                                  ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc        >  1:5243893/1‑93 (MQ=255)

                                                                                  ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc        >  2:5833655/1‑93 (MQ=255)

                                                                                   gCGATTATCGCC‑ACGGGCGGCGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt       <  2:1828498/93‑1 (MQ=255)

                                                                                    cGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTg      >  1:3572828/1‑93 (MQ=255)

                                                                                     gATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGa     >  1:376826/1‑93 (MQ=255)

                                                                                      aTTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTCGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt    >  1:8337619/1‑93 (MQ=255)

                                                                                        tATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATaa  >  2:169834/1‑93 (MQ=255)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA  >  NC_000913/4031891‑4032070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`