BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,139,669	+C	coding (233/330 nt) coding (132/159 nt)	yijI → b4558 ←	hypothetical protein hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,139,667	1	.	C	99.8%	1334.3 / ‑5.4	413	L77F (TTG→TTC) A45G (GCA→GGA)	yijI b4558	hypothetical protein hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (203/208); minor base T (0/1); total (203/210)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

Only 100 of 467 total aligned reads displayed.

GCTTCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑CCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGACGCGCAT  >  NC_000913/4139575‑4139754

gcTTCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTg                                                                                          <  2:3311931/93‑1 (MQ=255)

 cTTCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑c                                                                                        >  1:7849407/1‑93 (MQ=255)

 cTTCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑c                                                                                        >  2:8805514/1‑93 (MQ=255)

 cTTCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑c                                                                                        <  1:9273718/93‑1 (MQ=255)

   tCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑ccc                                                                                      <  2:2386367/93‑2 (MQ=255)

    ccTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑ccct                                                                                     >  1:7491273/1‑91 (MQ=255)

     cTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑ccctt                                                                                    >  2:5873656/1‑90 (MQ=255)

     cTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑ccctt                                                                                    <  1:430357/93‑4 (MQ=255)

     cTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑ccctt                                                                                    <  1:9875555/93‑4 (MQ=255)

      tGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑cccttg                                                                                   >  2:5006228/1‑89 (MQ=255)

        ttCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCg                                                                                  >  2:5424293/1‑93 (MQ=255)

        ttCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCg                                                                                  >  1:955738/1‑93 (MQ=255)

         tcgtcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCgg                                                                                 <  2:1668848/93‑1 (MQ=255)

         tcggcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCgg                                                                                 <  1:5113825/93‑1 (MQ=255)

           gtcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTc                                                                               >  2:2133635/1‑93 (MQ=255)

           gtcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTc                                                                               <  1:4853451/93‑1 (MQ=255)

            tcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCa                                                                              >  2:3063951/1‑93 (MQ=255)

            tcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCa                                                                              <  1:8298564/93‑1 (MQ=255)

            gcgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCa                                                                              <  1:4835907/92‑1 (MQ=255)

             cgGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAg                                                                             <  2:6314283/93‑1 (MQ=255)

              ggCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGt                                                                            <  1:1972638/93‑1 (MQ=255)

                ccccGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTgc                                                                          <  1:681398/93‑1 (MQ=255)

                ccccGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTgc                                                                          >  1:4081078/1‑93 (MQ=255)

                   cGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCa                                                                       >  2:9581950/1‑93 (MQ=255)

                   cGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCa                                                                       <  2:1071191/93‑1 (MQ=255)

                        accAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTa                                                                  <  2:2478962/91‑1 (MQ=255)

                        aTCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTa                                                                  >  2:510113/1‑93 (MQ=255)

                        aTCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTa                                                                  >  1:7678080/1‑93 (MQ=255)

                        aTCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTa                                                                  <  1:5977840/93‑1 (MQ=255)

                         tCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTAt                                                                 >  2:9747165/1‑93 (MQ=255)

                         tCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTAt                                                                 <  2:3671128/93‑1 (MQ=255)

                         tCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGCTAt                                                                 >  2:6089174/1‑93 (MQ=255)

                          cAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATg                                                                <  1:4953287/93‑1 (MQ=255)

                           aGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGc                                                               >  2:2071511/1‑93 (MQ=255)

                           aGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGc                                                               <  1:821184/93‑1 (MQ=255)

                            gTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGcc                                                              >  1:3414286/1‑93 (MQ=255)

                              aaCTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCAc                                                            >  2:2812251/1‑93 (MQ=255)

                              aaCTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCAc                                                            <  1:3067012/93‑1 (MQ=255)

                              aaCTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCAc                                                            >  2:6964751/1‑93 (MQ=255)

                               aCTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACt                                                           >  1:1167451/1‑93 (MQ=255)

                                cTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTc                                                          <  2:9026746/93‑1 (MQ=255)

                                cTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTc                                                          <  2:1348248/93‑1 (MQ=255)

                                    cAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTGTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTTAAtt                                                      <  2:7066627/93‑1 (MQ=255)

                                     aGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAAttt                                                     <  1:1410529/93‑1 (MQ=255)

                                      gTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTtg                                                    >  2:4835050/1‑93 (MQ=255)

                                      gTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTtg                                                    >  1:8484178/1‑93 (MQ=255)

                                      gTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTtg                                                    >  2:4639810/1‑93 (MQ=255)

                                       ttATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTtgt                                                   >  1:70921/1‑93 (MQ=255)

                                       ttATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTtgt                                                   <  1:906743/93‑1 (MQ=255)

                                        tATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGACACTCAATTtgtg                                                  >  2:8256323/1‑93 (MQ=255)

                                            gcccgccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGtgatga                                              <  1:805970/93‑1 (MQ=255)

                                            gcccgccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGACACTCAATTTGtgatga                                              >  2:116962/1‑93 (MQ=255)

                                              ccgccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGaaa                                            >  1:5441617/1‑93 (MQ=255)

                                               cgccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGaaaa                                           >  2:9536492/1‑93 (MQ=255)

                                               cgccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGaaaa                                           >  1:3917472/1‑93 (MQ=255)

                                               cgccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGaaaa                                           >  1:8616035/1‑93 (MQ=255)

                                                gccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGTAGGTTATGCCACTCAATTTGTGATGaaaaa                                          <  1:9769157/93‑1 (MQ=255)

                                                gccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGaaaaa                                          >  1:2135932/1‑93 (MQ=255)

                                                 ccAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAg                                         >  1:6940732/1‑93 (MQ=255)

                                                  cAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGt                                        <  1:5722878/93‑1 (MQ=255)

                                                     cGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTAtt                                     <  2:2176444/93‑1 (MQ=255)

                                                       tATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTac                                   <  2:7406780/93‑1 (MQ=255)

                                                        aTCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTaca                                  >  1:231194/1‑93 (MQ=255)

                                                        aTCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTaca                                  >  1:3323959/1‑93 (MQ=255)

                                                         tCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTacac                                 <  2:2979916/93‑1 (MQ=255)

                                                         tCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTacac                                 <  2:5602904/93‑1 (MQ=255)

                                                         tCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTacac                                 <  1:1667993/93‑1 (MQ=255)

                                                         tCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTacac                                 >  1:3586106/1‑93 (MQ=255)

                                                          cGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTAcaca                                <  2:658353/93‑1 (MQ=255)

                                                          cGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTAcaca                                <  1:6409515/93‑1 (MQ=255)

                                                            gTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAgg                              >  2:5208370/1‑92 (MQ=255)

                                                            gTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGc                              <  1:2021555/93‑1 (MQ=255)

                                                             tGCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCg                             >  2:3542600/1‑93 (MQ=255)

                                                              gCCAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGt                            <  1:2705464/93‑1 (MQ=255)

                                                               ccAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGtt                           >  1:8847565/1‑93 (MQ=255)

                                                                cAATCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGttt                          >  2:946471/1‑93 (MQ=255)

                                                                 aaTCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTc                         <  1:10016290/93‑1 (MQ=255)

                                                                 aaTCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTc                         <  1:3176239/93‑1 (MQ=255)

                                                                  aTCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCa                        <  2:8617579/93‑1 (MQ=255)

                                                                   tCAGCGCAAGGGCTCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCaa                       <  2:4133018/93‑1 (MQ=255)

                                                                   tCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCTCTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCaa                       <  2:9220945/93‑1 (MQ=255)

                                                                   tCAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCaa                       >  1:2938043/1‑93 (MQ=255)

                                                                    cAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAAc                      >  1:4422707/1‑93 (MQ=255)

                                                                    cAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAAc                      <  2:805852/93‑1 (MQ=255)

                                                                    cAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAAc                      <  1:6502460/93‑1 (MQ=255)

                                                                    cAGCGCAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAAc                      >  1:2578081/1‑93 (MQ=255)

                                                                      gcgcAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGt                    <  2:5685642/93‑1 (MQ=255)

                                                                      gcgcAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGt                    >  1:5555654/1‑93 (MQ=255)

                                                                       cgcAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTg                   <  2:7176620/93‑1 (MQ=255)

                                                                       cgcAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGCTTCAACGTg                   <  2:7570342/93‑1 (MQ=255)

                                                                         cAAGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGc                 <  2:1939792/93‑1 (MQ=255)

                                                                          aaGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCt                >  1:8941398/1‑93 (MQ=255)

                                                                          aaGGGCGCTTTGGGCGTGGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCt                <  2:3930936/93‑1 (MQ=255)

                                                                           aGGGCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCtt               <  1:383424/93‑1 (MQ=255)

                                                                             ggCGCTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAATGTGGCTTGc             >  1:8342046/1‑93 (MQ=255)

                                                                                 cTTTTGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGa         >  2:3156097/1‑93 (MQ=255)

                                                                                  ttttGGCGTTGCCCTTGCGGTCAGTGGGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGAc        <  1:150881/93‑1 (MQ=255)

                                                                                    ttGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGAcgc      >  1:2785848/1‑93 (MQ=255)

                                                                                     tGGCGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGAcgcg     >  2:6815513/1‑93 (MQ=255)

                                                                                        cGTTGCCCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAGAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGACGCGCAt  <  2:6672080/93‑1 (MQ=255)

GCTTCCTGTTCGTCGGCCCCGGAAATCAGTAACTGGCAGTTATCGCCCGCCAGCGTATCGGTGCCAATCAGCGCAAGGGCGCTTTTGGCGTTG‑CCTTGCGGTCAGTGCGCAGGTTATGCCACTCAATTTGTGATGAAAAAGTATTACACAGCGTTTCAACGTGGCTTGCCGGACGCGCAT  >  NC_000913/4139575‑4139754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`