BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	541,786	+G	coding (675/1146 nt)	ybbZ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	541,782	1	.	G	98.6%	685.2 / 1.1	216	coding (671/1146 nt)	ybbZ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (98/115); total (99/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 254 total aligned reads displayed.

CGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑GG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTA  >  NC_000913/541690‑541868

cGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACa                                                                                           <  2:4460147/93‑1 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGGGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg                                                                                        >  2:8067681/1‑93 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGGGTCTAGCCCCCCAAACAGGGCCACGCCGGAGAGGGCCGACGAACTTGACCacg                                                                                         >  1:9692683/1‑91 (MQ=255)

    gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg‑g‑g                                                                                    >  2:4252928/1‑93 (MQ=255)

      tAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg‑g‑ggc                                                                                  >  2:7998846/1‑91 (MQ=255)

      tAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg‑g‑ggc                                                                                  <  1:7909312/93‑3 (MQ=255)

        aCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg‑g‑ggctg                                                                                <  1:4009945/93‑5 (MQ=255)

          ccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCa                                                                               >  2:2787531/1‑93 (MQ=255)

           cGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCaa                                                                              >  2:5470823/1‑93 (MQ=255)

            gCTGGTAGGGGCACGCGGCGCGCCGGCGGTGTTTGGCCCGCAAAAACGGGCAACGCCGGCGCTGGTCGAAGAACTTGAACAGGG‑G‑GCTGCaaa                                                                             >  2:7868612/1‑93 (MQ=255)

              tGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAAt                                                                           >  2:1601776/1‑93 (MQ=255)

               ggTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAAtt                                                                          >  2:3002962/1‑93 (MQ=255)

               ggTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAAtt                                                                          <  2:7115543/93‑1 (MQ=255)

                gTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTa                                                                         <  1:7257656/93‑1 (MQ=255)

                 tAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTAc                                                                        <  2:9355789/93‑1 (MQ=255)

                  aGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACg                                                                       <  2:2086708/93‑1 (MQ=255)

                   ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGc                                                                      >  2:5886043/1‑93 (MQ=255)

                    gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGcc                                                                     <  1:6092521/93‑1 (MQ=255)

                    gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGcc                                                                     <  2:2652783/93‑1 (MQ=255)

                    gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGcc                                                                     <  2:1085768/93‑1 (MQ=255)

                      gCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCg                                                                   >  1:2437540/1‑93 (MQ=255)

                        aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCgtg                                                                 <  2:6049482/93‑1 (MQ=255)

                        aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCgtg                                                                 <  1:3411920/93‑1 (MQ=255)

                         cgcggcgcggCGGCGGTGTTTGGCCGGCAAAAGGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑GG‑G‑GCTGCAAAATTACGCCCGTGtt                                                               <  1:6951421/93‑1 (MQ=255)

                         cgcggcgcggCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCgtgt                                                                >  1:7590061/1‑93 (MQ=255)

                         cgcggcgcggCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCgtgt                                                                <  1:293143/93‑1 (MQ=255)

                         cgcggcgcggCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCgtgt                                                                <  2:8929767/93‑1 (MQ=255)

                           cggcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGttt                                                              <  2:1785403/93‑1 (MQ=255)

                             gcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTa                                                            >  1:31891/1‑93 (MQ=255)

                              cgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTac                                                           <  1:9475312/93‑1 (MQ=255)

                               gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTaca                                                          >  2:8231087/1‑93 (MQ=255)

                               gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTaca                                                          <  2:9031828/93‑1 (MQ=255)

                               gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTaca                                                          >  1:8660636/1‑93 (MQ=255)

                               gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTaca                                                          <  2:6940847/93‑1 (MQ=255)

                                cggcggcggTGTTTGGCCTGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaa                                                         <  1:6253788/93‑1 (MQ=255)

                                cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaa                                                         <  2:3618936/93‑1 (MQ=255)

                                 ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaac                                                        <  2:9823977/93‑1 (MQ=255)

                                 ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaac                                                        <  2:310520/93‑1 (MQ=255)

                                 ggcggcggTGTTGGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaac                                                        <  1:5719476/93‑1 (MQ=255)

                                  gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaaca                                                       <  1:3893797/93‑1 (MQ=255)

                                  gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaaca                                                       <  2:855293/93‑1 (MQ=255)

                                  gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTacaaca                                                       >  1:455378/1‑93 (MQ=255)

                                   cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTAAGCCCGTGTTTTacaacac                                                      >  2:2354099/1‑92 (MQ=255)

                                      cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCaa                                                   >  2:6148453/1‑93 (MQ=255)

                                       ggTGTTTGGCCCGTAAAAAGGGGTAACGCCGGAGATGGTCGAAGAACTTGAACA‑GG‑GTGCTGCAAAATTACGCCCGTGTTGTACAATAGCaaa                                                  >  2:8939887/1‑93 (MQ=255)

                                       ggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                  <  2:2299780/93‑1 (MQ=255)

                                       ggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                  >  1:2974457/1‑93 (MQ=255)

                                        gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCacac                                                 >  2:3306509/1‑93 (MQ=255)

                                        gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                 >  2:8268745/1‑93 (MQ=255)

                                         tgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACt                                                >  2:1021596/1‑93 (MQ=255)

                                          gtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                               >  1:10001278/1‑93 (MQ=255)

                                           tttGGCCCGCAAAAAGTGGCAACGCCGGAGATGGTCGAAGAACTTGAACC‑GGCG‑GCTGCAAAATTACGCCCGGGTTTTCCAGCAGAAAACTGa                                              >  2:5091496/1‑93 (MQ=255)

                                            ttGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaa                                             >  1:8712195/1‑93 (MQ=255)

                                             tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                            >  1:6562866/1‑93 (MQ=255)

                                             tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                            >  2:5380608/1‑93 (MQ=255)

                                             tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                            <  1:910781/93‑1 (MQ=255)

                                              ggCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAt                                           <  2:9153308/93‑1 (MQ=255)

                                               gCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAtt                                          <  1:1769828/93‑1 (MQ=255)

                                                 ccGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTaa                                        <  1:1238460/93‑1 (MQ=255)

                                                 ccGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTaa                                        <  1:4126069/93‑1 (MQ=255)

                                                   gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATg                                      <  1:8205091/93‑1 (MQ=255)

                                                    cAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                     <  2:3452933/93‑1 (MQ=255)

                                                     aaaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                    >  2:7428054/1‑93 (MQ=255)

                                                     aaaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                    <  1:7399384/93‑1 (MQ=255)

                                                      aaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCt                                   <  1:8488806/93‑1 (MQ=255)

                                                        aaGGGGCAACGCCGGAGATGCTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGc                                 <  2:1938898/93‑1 (MQ=255)

                                                           gggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAg                              >  1:8512978/1‑93 (MQ=255)

                                                            ggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                             <  1:1421481/93‑1 (MQ=255)

                                                             gCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                            <  1:5121153/93‑1 (MQ=255)

                                                             gCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                            <  2:4817106/93‑1 (MQ=255)

                                                             gCAACGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                            <  1:6149327/93‑1 (MQ=255)

                                                                aCGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggc                         >  1:4445781/1‑93 (MQ=255)

                                                                aCGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggc                         <  1:8989230/93‑1 (MQ=255)

                                                                aCGCCGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggc                         <  1:6009176/93‑1 (MQ=255)

                                                                aCGCCGGAGATGGTCGAAGAACTTGAACA‑GG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcg                        <  1:5550024/93‑1 (MQ=255)

                                                                   ccGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                      >  1:3570521/1‑93 (MQ=255)

                                                                    cGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                     <  1:9809082/93‑1 (MQ=255)

                                                                    cGGAGATGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                     <  2:9256936/93‑1 (MQ=255)

                                                                      gagaTGTGCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                   <  1:5535492/93‑1 (MQ=255)

                                                                      gagaTGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                   >  1:2034721/1‑93 (MQ=255)

                                                                      gagaTGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                   <  2:6272406/93‑1 (MQ=255)

                                                                        gaTGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                 >  2:2551261/1‑93 (MQ=255)

                                                                         aTGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcgg                <  1:6348406/93‑1 (MQ=255)

                                                                          tGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                             <  2:9656097/79‑1 (MQ=255)

                                                                          tGGTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                             >  1:9656098/1‑79 (MQ=255)

                                                                           ggTCGAAGAACTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGgcggcggcg              >  2:1323548/1‑93 (MQ=255)

                                                                               gaagaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgg          >  1:7975606/1‑92 (MQ=255)

                                                                               gaagaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgc          <  1:8816878/93‑1 (MQ=255)

                                                                                aagaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg         <  1:4845766/93‑1 (MQ=255)

                                                                                aagaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg         >  1:525108/1‑93 (MQ=255)

                                                                                 agaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg        <  2:201348/93‑1 (MQ=255)

                                                                                 agaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg        <  1:5672409/93‑1 (MQ=255)

                                                                                  gaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg       <  1:7081173/93‑1 (MQ=255)

                                                                                  gaaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg       >  1:8917684/1‑93 (MQ=255)

                                                                                   aaCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggg      >  2:7248876/1‑92 (MQ=255)

                                                                                    aCTTGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggcg     <  2:5351307/93‑1 (MQ=255)

                                                                                       tGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggcggca  >  2:9619656/1‑91 (MQ=255)

                                                                                       tGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTa  <  2:7730241/93‑1 (MQ=255)

                                                                                       tGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTa  <  1:9600392/93‑1 (MQ=255)

                                                                                       tGAACAGGG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTa  <  2:642865/93‑1 (MQ=255)

CGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑GG‑G‑GCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTA  >  NC_000913/541690‑541868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`