BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,311,496	+C	coding (301/408 nt)	phnQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,311,495	1	.	C	99.1%	1420.2 / 2.7	442	coding (302/408 nt)	phnQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (201/237); total (202/240)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 487 total aligned reads displayed.

GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑CGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG  >  NC_000913/4311403‑4311582

gAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑a                                                                                          >  2:4707929/1‑93 (MQ=255)

 aGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑c                                                                                        <  2:2084369/93‑1 (MQ=255)

    aaTGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑ccgt                                                                                     <  2:9818465/93‑4 (MQ=255)

       gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTcc                                                                                   >  2:3359537/1‑93 (MQ=255)

       gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTcc                                                                                   <  1:7496879/93‑1 (MQ=255)

       gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTcc                                                                                   <  1:4153767/93‑1 (MQ=255)

        aTTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCg                                                                                  >  1:9345944/1‑93 (MQ=255)

         ttAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCgg                                                                                 >  2:2259043/1‑93 (MQ=255)

          tAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGc                                                                                >  2:1776500/1‑93 (MQ=255)

          tAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGc                                                                                >  2:9275907/1‑93 (MQ=255)

            aTGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAAGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGccc                                                                              >  2:3195106/1‑93 (MQ=255)

            aTGTGGATAGAGTGTCTTTTTGCGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACTCTGCTCACGCTGT‑CTC‑C‑CGTTCCGGccc                                                                              >  1:1241772/1‑93 (MQ=255)

             tgtgGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCa                                                                             <  1:8414109/93‑1 (MQ=255)

                ggATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGc                                                                          <  1:5564281/93‑1 (MQ=255)

                  aTAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCATGCTGT‑C‑C‑ACCGTTTCGGCCCAGGCCg                                                                        >  2:1918570/1‑93 (MQ=255)

                  aTAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCg                                                                        >  1:3217922/1‑93 (MQ=255)

                  aTAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCg                                                                        >  2:7379710/1‑93 (MQ=255)

                  aTAGAGTTACTTTTAGAGGTTGCGTAATTATACATTTGCCGCTATTTCTTTTATTCGCCCTGCCCACGGTTTGC‑G‑C‑CGTTCAGGCCCAGGctg                                                                        <  2:7655260/93‑3 (MQ=255)

                   tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGATCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGa                                                                       >  1:4190505/1‑93 (MQ=255)

                     gagTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGata                                                                     <  2:9028839/93‑1 (MQ=255)

                       gTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatt                                                                   >  2:7053886/1‑93 (MQ=255)

                        tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatta                                                                  >  1:8165479/1‑93 (MQ=255)

                        tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatta                                                                  >  2:2770786/1‑93 (MQ=255)

                        tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatta                                                                  >  2:3049331/1‑93 (MQ=255)

                         ttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtattat                                                                 <  2:5451635/93‑1 (MQ=255)

                          tCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATc                                                                >  2:2455351/1‑93 (MQ=255)

                             ttttGAGGTTGGCTAATGAAACTCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgac                                                             >  1:1815718/1‑93 (MQ=255)

                             ttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgac                                                             >  1:9085468/1‑93 (MQ=255)

                               ttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgacga                                                           <  1:4929468/93‑1 (MQ=255)

                               ttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgacga                                                           >  2:9486568/1‑93 (MQ=255)

                               ttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgacga                                                           <  2:8522882/93‑1 (MQ=255)

                                 gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCTCCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGaca                                                         <  2:793682/93‑1 (MQ=255)

                                 gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGaca                                                         <  1:6245000/93‑1 (MQ=255)

                                  aGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGacac                                                        >  1:5750321/1‑93 (MQ=255)

                                   ggTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACAcc                                                       >  1:3077086/1‑93 (MQ=255)

                                    gTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCa                                                      >  2:4484719/1‑93 (MQ=255)

                                     ttGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAt                                                     <  2:5019945/93‑1 (MQ=255)

                                     ttGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAt                                                     <  2:5972434/93‑1 (MQ=255)

                                      tGGCTAATGAAACGCTTTCCGCTATTTCTTTTAGTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAtt                                                    <  1:9713207/93‑1 (MQ=255)

                                       ggCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTg                                                   <  1:2657241/93‑1 (MQ=255)

                                          tAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCAGGGCCAGGACGATATTATCGACCACACAATTGGCa                                                >  2:7508739/1‑93 (MQ=255)

                                           aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  2:1685981/1‑93 (MQ=255)

                                           aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  2:8158901/1‑93 (MQ=255)

                                           aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  1:2298435/1‑93 (MQ=255)

                                             tGAAACGCTTTCCCCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAata                                             <  1:5898846/93‑1 (MQ=255)

                                                 aCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTca                                         <  2:4880943/93‑1 (MQ=255)

                                                  cGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcag                                        >  1:7612697/1‑93 (MQ=255)

                                                   gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                                       <  1:9578822/93‑1 (MQ=255)

                                                   gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                                       <  2:3746999/93‑1 (MQ=255)

                                                    cTTTCCGTTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  1:9464428/93‑1 (MQ=255)

                                                    cTTTCCGCTATTTCTTTTATTCCCCCTACTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  2:1497048/93‑1 (MQ=255)

                                                    cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  1:3886561/93‑1 (MQ=255)

                                                    cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  2:4370690/93‑1 (MQ=255)

                                                    cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  1:6703672/93‑1 (MQ=255)

                                                     tttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     >  1:2934528/1‑93 (MQ=255)

                                                     tttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     >  2:8121257/1‑93 (MQ=255)

                                                     tttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTACCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     <  2:9597284/93‑1 (MQ=255)

                                                      ttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTGAGCAgg                                    <  1:1670858/93‑1 (MQ=255)

                                                       tCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTTGCAATATTCAGCAGGc                                   >  2:57194/1‑93 (MQ=255)

                                                          gCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAt                                >  1:7239431/1‑93 (MQ=255)

                                                           cTATTTCTTCTATTCACCCTGCCCACCCTTTCC‑C‑C‑CGTTCCGGCCCAGGCCGATATTATCGACGACCCCATTGTCAATATTCAGCAGGCGAtt                               <  1:1104569/93‑1 (MQ=255)

                                                            tATTTCTTTTATTCCCCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  2:3282885/93‑1 (MQ=255)

                                                            tATTTCTTTTATTCACCCTGCTCACGCTTC‑C‑C‑ACCGTTCCGTCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  1:1838098/93‑1 (MQ=255)

                                                            tATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  2:1571538/93‑1 (MQ=255)

                                                            tATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  1:4232819/93‑1 (MQ=255)

                                                            tATTTCTTTTATTCACCCTGCTCACGCCGT‑C‑CTC‑CGTTCCGGCCCAGGCCGATATTATCGACGGCACCATTGGCAATATTCAGCAGGCGATTa                              <  2:6674631/93‑1 (MQ=255)

                                                             aTTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             <  1:1541985/93‑1 (MQ=255)

                                                             aTTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             <  1:3694356/93‑1 (MQ=255)

                                                              tttCTTTTATTCACCCTGCTCACTCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            <  2:2639211/93‑1 (MQ=255)

                                                              tttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            >  2:781504/1‑93 (MQ=255)

                                                              tttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            <  1:9904686/93‑1 (MQ=255)

                                                              tttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            <  1:8719165/93‑1 (MQ=255)

                                                               ttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATg                           >  1:6481451/1‑93 (MQ=255)

                                                                tCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGa                          >  1:4386667/1‑93 (MQ=255)

                                                                  ttttATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACg                        >  2:1182799/1‑93 (MQ=255)

                                                                    ttATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      <  1:6113306/93‑1 (MQ=255)

                                                                    ttATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      <  1:4500758/93‑1 (MQ=255)

                                                                      aTTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTta                    >  1:5081886/1‑93 (MQ=255)

                                                                       ttCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtat                   <  2:8383432/93‑1 (MQ=255)

                                                                       ttCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtat                   <  1:7957209/93‑1 (MQ=255)

                                                                        tcgcccTGCTCCCGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtata                  <  2:6910825/90‑1 (MQ=255)

                                                                        tCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtata                  <  1:5231644/93‑1 (MQ=255)

                                                                          ccccTGCTAACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAc                <  1:1948375/92‑1 (MQ=255)

                                                                            ccTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAccc              >  2:6791474/1‑93 (MQ=255)

                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            >  2:5473596/1‑93 (MQ=255)

                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            >  1:4687399/1‑93 (MQ=255)

                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            <  1:7009657/93‑1 (MQ=255)

                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            <  2:3396688/93‑1 (MQ=255)

                                                                               gCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGa           <  1:10018939/93‑1 (MQ=255)

                                                                                cTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAc          <  1:3307158/93‑1 (MQ=255)

                                                                                cTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAc          >  2:5242572/1‑93 (MQ=255)

                                                                                 tCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAcc         >  2:3584130/1‑93 (MQ=255)

                                                                                   aCGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       >  2:8873994/1‑93 (MQ=255)

                                                                                   aCGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       >  2:4092/1‑93 (MQ=255)

                                                                                    cGCTGT‑C‑C‑ACCGTGCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTg      <  1:4419895/93‑1 (MQ=255)

                                                                                      cTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTggg    <  2:7110040/93‑1 (MQ=255)

                                                                                       tGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   >  1:2132539/1‑93 (MQ=255)

                                                                                       tGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   <  1:1497240/93‑1 (MQ=255)

                                                                                       tGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   <  2:3480364/93‑1 (MQ=255)

                                                                                        gT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCg  >  2:4248818/1‑93 (MQ=255)

GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑CGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG  >  NC_000913/4311403‑4311582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`