BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,449,738	+G	coding (1106/1254 nt)	ytfR →	YtfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,449,737	1	.	G	99.7%	1082.4 / ‑5.4	336	C369G (TGC→GGC)	ytfR	YtfR
Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (164/171); minor base C (1/0); total (165/171)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 377 total aligned reads displayed.

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824

gCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct                                                                                          <  1:4004603/93‑1 (MQ=255)

  ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑gg                                                                                       >  2:357334/1‑92 (MQ=255)

     tGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggcta                                                                                    <  1:3762647/93‑5 (MQ=255)

     tGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggcta                                                                                    <  1:3522845/93‑5 (MQ=255)

        ccGGAAGACCGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCCGGCCCAGCGCGGCTGGCTACgc                                                                                  >  2:4030265/1‑92 (MQ=255)

        ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGt                                                                                  >  2:3141332/1‑93 (MQ=255)

        ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGt                                                                                  >  2:503470/1‑93 (MQ=255)

          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTcc                                                                                <  1:6431013/93‑1 (MQ=255)

          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTcc                                                                                >  1:1323689/1‑93 (MQ=255)

          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTcc                                                                                >  2:8388015/1‑93 (MQ=255)

           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               <  1:3257201/93‑1 (MQ=255)

           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               <  1:2249525/93‑1 (MQ=255)

            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccct                                                                              >  1:300741/1‑92 (MQ=255)

            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              >  1:2111571/1‑93 (MQ=255)

            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              <  2:1044740/93‑1 (MQ=255)

             aGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAt                                                                             >  1:8018523/1‑93 (MQ=255)

              gACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAtt                                                                            <  1:423141/93‑1 (MQ=255)

                 aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTcc                                                                         >  2:7342282/1‑93 (MQ=255)

                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        <  1:2330116/93‑1 (MQ=255)

                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        >  2:9185721/1‑93 (MQ=255)

                     aaaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCa                                                                     >  2:7281279/1‑93 (MQ=255)

                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  <  2:8188066/93‑1 (MQ=255)

                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 <  2:3027294/93‑1 (MQ=255)

                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 <  1:7067838/93‑1 (MQ=255)

                          gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGaa                                                                >  1:9077917/1‑93 (MQ=255)

                          gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGaa                                                                >  1:9962583/1‑93 (MQ=255)

                          gATGGCATCATCGCTGCCGCCTCGGGGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAgca                                                                >  2:6651571/1‑91 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               <  1:5908782/93‑1 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               >  1:267461/1‑93 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               <  2:8280991/93‑1 (MQ=255)

                            ttgCATCACCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              <  2:7497796/91‑1 (MQ=255)

                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              <  2:6284232/93‑1 (MQ=255)

                              gCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGc                                                            <  1:8950230/93‑1 (MQ=255)

                              gCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGc                                                            <  1:1631443/93‑1 (MQ=255)

                               catcatCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                           <  2:1804276/93‑1 (MQ=255)

                                atcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCaa                                                          <  1:3104824/93‑1 (MQ=255)

                                atcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCaa                                                          >  2:3888675/1‑93 (MQ=255)

                                atcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCaa                                                          >  2:6413953/1‑93 (MQ=255)

                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCCGGACCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAGAGCAag                                                         >  1:150092/1‑93 (MQ=255)

                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  1:6750940/1‑93 (MQ=255)

                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         <  2:8219565/93‑1 (MQ=255)

                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  1:4890150/1‑93 (MQ=255)

                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  1:6224446/1‑93 (MQ=255)

                                  catcGCTGCCGCCTCGGTGCGGGGAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCGACGTCGCATTTCCCGCATACAACAGCAaga                                                        >  1:811550/1‑93 (MQ=255)

                                   atcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAagag                                                       >  2:3985972/1‑93 (MQ=255)

                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      >  1:4279458/1‑93 (MQ=255)

                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      <  2:5626739/93‑1 (MQ=255)

                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  1:4529620/93‑1 (MQ=255)

                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  1:6940052/93‑1 (MQ=255)

                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGC‑AGAGAtt                                                    <  2:3426776/93‑1 (MQ=255)

                                      gCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAtt                                                    >  1:73559/1‑93 (MQ=255)

                                       cTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                   <  2:6194254/93‑1 (MQ=255)

                                        tGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGc                                                  >  1:1032910/1‑93 (MQ=255)

                                            gccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                              >  2:325472/1‑93 (MQ=255)

                                            gccTCGGTGCGGGAAAATATCATACTCGCTCTCCCAGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                              >  1:1766854/1‑93 (MQ=255)

                                             ccTCGGTGCGGAAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             <  2:9838612/93‑1 (MQ=255)

                                             ccTAGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             >  1:7716893/1‑93 (MQ=255)

                                               tCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                           >  1:4469340/1‑93 (MQ=255)

                                                cGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCt                                          <  1:5744613/93‑1 (MQ=255)

                                                 ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                         <  2:9086626/93‑1 (MQ=255)

                                                  gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCttt                                        <  1:4761404/93‑1 (MQ=255)

                                                   tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCTTTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                       >  1:9797727/1‑93 (MQ=255)

                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     <  2:4803602/93‑1 (MQ=255)

                                                      gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                    <  2:9278852/93‑1 (MQ=255)

                                                       ggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                   >  1:8175643/1‑93 (MQ=255)

                                                             tatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                             <  2:7866428/93‑1 (MQ=255)

                                                             tatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                             >  2:5404026/1‑93 (MQ=255)

                                                              atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                            <  2:7105045/93‑1 (MQ=255)

                                                                 atcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         <  2:6092464/93‑1 (MQ=255)

                                                                 atcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         >  2:3680720/1‑93 (MQ=255)

                                                                  tcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCa                        >  2:2079407/1‑93 (MQ=255)

                                                                   ccTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAt                       <  1:5259167/93‑1 (MQ=255)

                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  2:5247891/93‑1 (MQ=255)

                                                                     ccGCTCCCCGGGCCCAGCGGTGCTGGCTACGTCCCATTTCCCGCAAAGACCAGCAAGAGATTGCC‑AACGCTTTATCCGCCAGCTTGGCATTCg                    <  1:5499423/92‑1 (MQ=255)

                                                                      cGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCg                    >  2:5820832/1‑93 (MQ=255)

                                                                      cGCTCTCCAGGCCCAGCGCGGCTCGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCg                    >  2:6939083/1‑93 (MQ=255)

                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  <  2:5299612/93‑1 (MQ=255)

                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  <  1:6464037/93‑1 (MQ=255)

                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  >  1:2891408/1‑93 (MQ=255)

                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac               >  1:511440/1‑93 (MQ=255)

                                                                            ccAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACACCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              >  1:8564879/1‑93 (MQ=255)

                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             <  2:2206449/93‑1 (MQ=255)

                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             <  1:7567662/93‑1 (MQ=255)

                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             >  1:9290081/1‑93 (MQ=255)

                                                                             cAGCCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             >  2:2679048/1‑93 (MQ=255)

                                                                              aGGCCCAGCGCGGCTGGCTACGTCCCACTTCCCGCAAAGAACAGCAAGAGATTGCCGACCGCTTTATCCGCCAGCTTGGCATTCGCACACCtt            >  1:2563324/1‑93 (MQ=255)

                                                                                 cccAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCaa         <  2:1133094/93‑1 (MQ=255)

                                                                                 cccAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCaa         <  2:5680536/93‑1 (MQ=255)

                                                                                 cccAGCGCGGCTGGCTACGTCCCATTTCCAGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCaa         <  2:2467954/93‑1 (MQ=255)

                                                                                  ccAGCGCGGCTGGCTCCGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAc        <  1:3319808/93‑1 (MQ=255)

                                                                                   cAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       <  2:1509718/93‑1 (MQ=255)

                                                                                   cAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       >  1:2217649/1‑93 (MQ=255)

                                                                                    aGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg      >  1:7186692/1‑93 (MQ=255)

                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACCCCTTCAACTGa     <  2:965376/93‑1 (MQ=255)

                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  1:2810796/1‑93 (MQ=255)

                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  2:8675974/1‑93 (MQ=255)

                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  2:4245291/1‑93 (MQ=255)

                                                                                       gcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaac   <  1:1010111/93‑1 (MQ=255)

                                                                                        cgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaaca  <  1:2548533/93‑1 (MQ=255)

                                                                                        cgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaaca  <  2:4556879/93‑1 (MQ=255)

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`