| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,096,260 | (G)7→8 | intergenic (+208/+528) | ycdU → / ← serX | hypothetical protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,096,253 | 1 | . | G | 81.2% | 32.5 / 4.9 | 16 | intergenic (+201/+535) | ycdU/serX | hypothetical protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (1/12); total (3/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGG‑ATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACC > NC_000913/1096161‑1096342 | ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTGCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGa < 1:8709591/93‑1 (MQ=255)ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGa < 1:2096765/93‑1 (MQ=255)ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGa < 2:2546104/93‑1 (MQ=255) cTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGa < 1:9639268/93‑1 (MQ=255) cTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑g < 1:3216720/93‑1 (MQ=255) cTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑g < 1:7223364/93‑1 (MQ=255) cTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑g < 2:5483123/93‑1 (MQ=255) cTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑‑g > 1:2309402/1‑93 (MQ=255) tAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gg < 1:2671470/93‑1 (MQ=255) tAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gg < 2:5871184/93‑1 (MQ=255) tAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gg < 1:4572452/93‑1 (MQ=255) tAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gg > 1:57454/1‑93 (MQ=255) aCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggg < 1:8430028/93‑1 (MQ=255) aCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggg < 2:535473/93‑1 (MQ=255) aCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggg < 2:5444386/93‑1 (MQ=255) cAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggg < 2:314503/93‑1 (MQ=255) aggcGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggg < 2:2779483/90‑1 (MQ=255) aGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggg > 1:6689250/1‑93 (MQ=255) aGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggg < 1:4521865/93‑1 (MQ=255) aGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGT‑‑gggggg > 2:336568/1‑93 (MQ=255) aGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAACATGGCGGTGA‑‑gggggg < 2:6705960/93‑1 (MQ=255) gccGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggg < 2:3235483/91‑1 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggg < 1:6879877/93‑1 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggg > 2:6098998/1‑93 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggg > 2:6098999/1‑93 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggg < 2:7695334/93‑1 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGAGCAATAAACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGACGGTGA‑‑ggggggg < 2:8920497/93‑1 (MQ=255) aCGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggggg < 2:5521488/93‑2 (MQ=255) aCGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggggg < 2:2401600/93‑2 (MQ=255) cGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggggga < 2:9845604/93‑3 (MQ=255) cGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑‑gggggggga > 1:4279831/1‑91 (MQ=255) gTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggat < 1:7624613/93‑4 (MQ=255) gTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggat < 1:6155616/93‑4 (MQ=255) gTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggat < 1:5887407/93‑4 (MQ=255) gTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggat < 2:2561061/93‑4 (MQ=255) tGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggggg > 2:199847/1‑89 (MQ=255) tGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggatt > 1:10016457/1‑89 (MQ=255) tGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGG‑GTTg > 2:2494313/1‑93 (MQ=255) tGGGCGGGGGCACCCGGGGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTCAATATGGCGGTGC‑‑ggggggggggt > 1:3033952/1‑89 (MQ=14) ggggggggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGa < 1:7431761/90‑1 (MQ=255) ggCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGa < 1:6256818/93‑1 (MQ=255) gCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGAc < 1:1551715/93‑1 (MQ=255) gCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGTATTGAc < 2:9758054/93‑1 (MQ=255) cGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCCTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGAcc > 2:293533/1‑92 (MQ=14) gggggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtc < 2:503447/93‑1 (MQ=255) gggggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtc < 1:7652252/93‑1 (MQ=255) gggggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtc < 1:6155017/93‑1 (MQ=255) gggggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtc < 2:7947180/93‑1 (MQ=255) ggggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtcg > 2:8487229/1‑93 (MQ=255) ggCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtcgct < 1:6189557/93‑1 (MQ=255) gCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtcgctt < 2:138261/93‑1 (MQ=16) cACCGGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtcgcttc < 2:3987762/93‑1 (MQ=255) cACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGG‑ATTGACtcgctt < 1:4858880/93‑1 (MQ=11) cACCCGGTGTCTACAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAG‑GGGGGGG‑ATTGACtcgcttc < 2:7234381/93‑1 (MQ=11) gTGA‑‑GGGGGGG‑ATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCCCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCCGTTGTCggac > 2:6133227/1‑90 (MQ=0) | CCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGG‑ATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACC > NC_000913/1096161‑1096342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |