BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,968	+G	coding (1132/1386 nt)	hofB ←	HofB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,964	1	.	G	98.4%	808.4 / 3.3	252	coding (1136/1386 nt)	hofB	HofB
Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (104/144); total (107/145)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 294 total aligned reads displayed.

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

gACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑t                                                                                             >  2:6706361/1‑93 (MQ=255)

  cGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg                                                                                          <  1:3842890/93‑1 (MQ=255)

  cGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg                                                                                          <  1:8845966/93‑1 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g‑g                                                                                      >  2:1080498/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g‑g                                                                                      >  1:9109528/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g‑g                                                                                      >  2:8204142/1‑93 (MQ=255)

     aTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g‑gg                                                                                     <  1:2058124/93‑2 (MQ=255)

       gACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g‑ggca                                                                                   <  1:8716063/93‑4 (MQ=255)

          cGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAgcg                                                                                <  2:3461170/93‑1 (MQ=255)

            gCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAgcggc                                                                              <  1:1618935/93‑1 (MQ=255)

            gCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAgcggc                                                                              >  1:6181395/1‑93 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGa                                                                            <  1:1448594/93‑1 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGa                                                                            <  1:677106/93‑1 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑GAG‑CAGCGGCGa                                                                            >  2:6587481/1‑93 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGAt                                                                           >  2:743732/1‑93 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGAt                                                                           >  2:1332241/1‑93 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGAt                                                                           <  2:4750537/93‑1 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGAt                                                                           >  2:3265688/1‑93 (MQ=255)

                tATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATg                                                                          >  1:2521668/1‑93 (MQ=255)

                  tGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGc                                                                        >  2:3540292/1‑93 (MQ=255)

                    ggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCa                                                                      >  1:10044656/1‑93 (MQ=255)

                    ggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCa                                                                      <  2:5097703/93‑1 (MQ=255)

                     gCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCAt                                                                     <  2:2394908/93‑1 (MQ=255)

                      cAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATa                                                                    <  1:7775221/93‑1 (MQ=255)

                      cAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATa                                                                    >  2:8910560/1‑93 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATAc                                                                   <  1:4416636/93‑1 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATAc                                                                   >  2:7733207/1‑93 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACa                                                                  >  1:9990257/1‑93 (MQ=255)

                         aaCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACAt                                                                 >  2:432465/1‑93 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTc                                                             >  2:289050/1‑93 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTc                                                             <  1:4147695/93‑1 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTc                                                             <  2:533264/93‑1 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGAGGCGATGGACATACATTGTc                                                             >  2:5374862/1‑93 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTg                                                           <  2:125100/93‑1 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTgg                                                          <  1:5198976/93‑1 (MQ=255)

                                 aTAAGGCCGTACGACCATAAAAACCGTGGGAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GGTG‑G‑CAGCGGCGATGGCCATACATTGTCTGGa                                                         >  2:1601461/1‑93 (MQ=255)

                                  tAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGaa                                                        >  2:7387579/1‑93 (MQ=255)

                                   aaGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAAt                                                       <  1:4741715/93‑1 (MQ=255)

                                   aaGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAAt                                                       <  2:8662653/93‑1 (MQ=255)

                                     ggCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAAtgt                                                     >  2:3714500/1‑93 (MQ=255)

                                       ccGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTgg                                                   <  2:1899944/93‑1 (MQ=255)

                                       ccGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTgg                                                   >  2:5540350/1‑93 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  <  1:4993036/93‑1 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  <  2:8693853/93‑1 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  <  1:7873410/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 <  1:5490592/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 >  1:6543410/1‑93 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGGGGag                                                 >  1:3332159/1‑92 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                >  1:1516299/1‑93 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                >  1:6133004/1‑93 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                <  1:4046052/93‑1 (MQ=255)

                                          gtcGACCATAAAAACCGGGGTAGTAATGTTCACCACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCAGACATTGTCTGGAATGTGGATg                                                <  1:2299676/91‑1 (MQ=255)

                                             gACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGc                                             <  2:2202316/93‑1 (MQ=255)

                                              aCCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCt                                            <  2:2079565/93‑1 (MQ=255)

                                               ccATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTc                                           <  1:6053329/93‑1 (MQ=255)

                                               ccATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTc                                           >  1:5669063/1‑93 (MQ=255)

                                                cATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcc                                          <  2:6134196/93‑1 (MQ=255)

                                                 aTAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                         <  1:2193598/93‑1 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTctcc                                        >  1:7058446/1‑93 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        >  1:4289491/1‑93 (MQ=255)

                                                   aaaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCt                                       <  2:4509656/93‑1 (MQ=255)

                                                   aaaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCt                                       <  1:4647188/93‑1 (MQ=255)

                                                    aaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCtt                                      <  2:3892481/93‑1 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   <  2:7265012/93‑1 (MQ=255)

                                                        ccGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgctg                                  >  2:5678004/1‑93 (MQ=255)

                                                            ggTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCgg                              <  1:6902110/93‑1 (MQ=255)

                                                              tAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCg                            >  2:9671760/1‑93 (MQ=255)

                                                              tAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCg                            >  2:317035/1‑93 (MQ=255)

                                                              tAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCg                            >  1:955827/1‑93 (MQ=255)

                                                              tAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTG‑CGGCGa                           >  2:7873252/1‑93 (MQ=255)

                                                               aGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGa                           <  2:1151314/93‑1 (MQ=255)

                                                               aGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑G‑GTCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGa                           >  2:6375611/1‑93 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        >  2:9157219/1‑93 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  2:7484503/93‑1 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  2:1438998/93‑1 (MQ=255)

                                                                  aaTGGACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  1:5938299/93‑1 (MQ=255)

                                                                   aTGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAt                       <  1:9239629/93‑1 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      >  2:3202578/1‑93 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      >  1:3051305/1‑93 (MQ=255)

                                                                         cgaaCGGGGTGCCTGGAAGAG‑GG‑G‑G‑CAGCGCAGATGGCCGAACATTGTGTGGAATGGGGATGGGCTCCCCTTGCTGCCGGGGACAATGTGGGc                 <  2:2929327/91‑1 (MQ=255)

                                                                         acaACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGc                 <  2:2622708/93‑1 (MQ=255)

                                                                          caACCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCa                >  1:8164096/1‑93 (MQ=255)

                                                                           aaCCGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCaa               >  2:1798030/1‑93 (MQ=255)

                                                                             ccGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAg             >  2:4604487/1‑93 (MQ=255)

                                                                              cGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGt            >  1:3596887/1‑93 (MQ=255)

                                                                              cGGGTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGt            >  2:701406/1‑93 (MQ=255)

                                                                               gggTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGtt           <  1:4912034/93‑1 (MQ=255)

                                                                               gggTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGtt           >  1:8589204/1‑93 (MQ=255)

                                                                                ggTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGttt          >  2:6240728/1‑93 (MQ=255)

                                                                                ggTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGttt          >  1:2986658/1‑93 (MQ=255)

                                                                                 gTGCCTGCCAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTg         <  1:7366874/93‑1 (MQ=255)

                                                                                  tgcctgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGc        <  1:8948724/93‑1 (MQ=255)

                                                                                  tgcctgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGc        <  2:1854513/93‑1 (MQ=255)

                                                                                  tgcctgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGc        <  2:6046190/93‑1 (MQ=255)

                                                                                  tgcctgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGc        >  1:10014301/1‑93 (MQ=255)

                                                                                    cctgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGt      <  2:7075486/93‑1 (MQ=255)

                                                                                    cctgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGt      <  1:1187058/93‑1 (MQ=255)

                                                                                      tgccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAc    <  1:22588/93‑1 (MQ=255)

                                                                                       gccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAcc   >  1:9054151/1‑93 (MQ=255)

                                                                                       gccAG‑TGGG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAcc   <  2:4266989/93‑1 (MQ=255)

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑G‑G‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`