BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,472,339	(C)_6→7	coding (95/861 nt)	ydbC →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,472,333	1	.	C	96.2%	557.3 / 13.1	184	coding (89/861 nt)	ydbC	putative dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (5/2); new base C (64/111); total (71/113)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

TGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑CC‑CCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTA  >  NC_000913/1472241‑1472420

tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTg‑‑g                                                                                            >  1:9580370/1‑93 (MQ=255)

tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTg‑‑g                                                                                            <  2:4120141/93‑1 (MQ=255)

 gCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑c                                                                                         >  2:1540238/1‑93 (MQ=255)

 gCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑c                                                                                         <  2:1913066/93‑1 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑tc‑c                                                                                      >  2:9725124/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑c                                                                                      <  2:9215396/93‑1 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑c                                                                                      >  2:2862424/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑GTTTTG‑‑G‑‑cc‑c                                                                                      >  1:3745033/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTGGGTTATGGCGCGATGCAACTGGCAGGTGCTGGA‑G‑TTTTTG‑‑G‑‑ac‑c                                                                                      >  1:5645390/1‑93 (MQ=255)

     tGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccc                                                                                    >  1:1392684/1‑93 (MQ=255)

      gAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccc                                                                                   <  2:2487583/93‑1 (MQ=255)

       agcagcAATACCTTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccc                                                                                  <  2:3287374/93‑2 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccc                                                                                 >  2:5423662/1‑91 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccca                                                                                 <  1:4493252/93‑3 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccca                                                                                 <  1:5633551/93‑3 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGGTACCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccca                                                                                 <  1:1498954/93‑3 (MQ=255)

         cagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccac                                                                                <  2:1432328/93‑4 (MQ=255)

         cagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccac                                                                                <  2:3676748/93‑4 (MQ=255)

          agcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccacg                                                                               >  2:806163/1‑89 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACgaga                                                                             >  2:615387/1‑93 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACgaga                                                                             >  2:9589067/1‑93 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACgaga                                                                             <  1:3539901/93‑1 (MQ=255)

              aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGAt                                                                            >  2:6043147/1‑93 (MQ=255)

               tACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATc                                                                           <  1:458909/93‑1 (MQ=255)

                aCATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCg                                                                          >  1:3841608/1‑93 (MQ=255)

                 cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGc                                                                         <  1:1522109/93‑1 (MQ=255)

                  aTTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGcc                                                                        >  2:389544/1‑93 (MQ=255)

                    ttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGccgc                                                                      <  1:467598/93‑3 (MQ=255)

                     tACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCcgcg                                                                     >  1:3389584/1‑93 (MQ=255)

                      aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGt                                                                    <  1:8601745/93‑1 (MQ=255)

                         ctcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGc                                                                 >  1:7724709/1‑93 (MQ=255)

                          tcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCt                                                                >  1:3056020/1‑93 (MQ=255)

                             gTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGCCGCtata                                                             >  1:7147148/1‑93 (MQ=255)

                               aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GA‑CC‑CCCCACGAGATCGCCGCGTCGCTATAAc                                                           >  1:9158212/1‑93 (MQ=255)

                                 aaaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑CC‑CCCCACGAGATCGCCACGTCGCTATAACCGt                                                        >  2:6268188/1‑93 (MQ=255)

                                  aaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGt                                                        >  1:6080046/1‑93 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGCTCGCCGCGTCGCTATAACCGtgc                                                      >  2:6937268/1‑93 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                      <  2:7805686/93‑1 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                      <  1:1179006/93‑1 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                      >  2:4963025/1‑93 (MQ=255)

                                     tCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgct                                                     >  1:6925126/1‑93 (MQ=255)

                                     tCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑GTTTTG‑‑G‑‑CCTCCCCACGAGATGGCCGGGTCGCTATAACCGtgcg                                                     >  2:6492197/1‑92 (MQ=255)

                                     accGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgct                                                     <  2:2570190/92‑1 (MQ=255)

                                         ttAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGt                                                 <  2:8890295/93‑1 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGG‑TTTTCG‑‑C‑‑CC‑CCCCACGAGATCGGCGCGTCGCTATAACCGTGCTGCGTg                                                >  2:6665199/1‑93 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTg                                                <  1:8407834/93‑1 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTg                                                <  1:1305291/93‑1 (MQ=255)

                                           aaCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGa                                               >  1:542269/1‑93 (MQ=255)

                                            aCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAg                                              <  1:1606149/93‑1 (MQ=255)

                                            aCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAg                                              <  2:7444145/93‑1 (MQ=255)

                                             ccGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAgg                                             <  2:9123530/93‑1 (MQ=255)

                                             ccGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAgg                                             <  2:1073034/93‑1 (MQ=255)

                                              cGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgc                                            <  2:2827125/93‑1 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          >  1:892352/1‑93 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  2:8230813/93‑1 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  2:9537425/93‑1 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  1:4875409/93‑1 (MQ=255)

                                                 cTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTGCCC‑‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  1:9019521/93‑1 (MQ=255)

                                                 cTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCt                                         <  1:4399262/93‑1 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                        >  1:2562155/1‑93 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                        >  2:7629988/1‑93 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                       >  1:6542054/1‑93 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                       <  2:5127810/93‑1 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                       >  2:6872453/1‑93 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                     <  2:167503/93‑1 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                     <  2:6085172/93‑1 (MQ=255)

                                                      ttATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAt                                    <  2:1379335/93‑1 (MQ=255)

                                                        aTGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTg                                  >  1:2153055/1‑93 (MQ=255)

                                                        aTGGCGCCATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTg                                  <  1:5788492/93‑1 (MQ=255)

                                                          ggCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTggg                                >  1:995181/1‑93 (MQ=255)

                                                              cgATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                            >  2:8505885/1‑93 (MQ=255)

                                                              cgATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                            <  2:3240604/93‑1 (MQ=255)

                                                              cgATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                            <  1:4304192/93‑1 (MQ=255)

                                                                aTGCAACTGGCAGGTCCTGGA‑GTTTTTTG‑‑C‑‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa                          >  2:2617211/1‑93 (MQ=255)

                                                                aTGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa                          >  1:6424808/1‑93 (MQ=255)

                                                                 tGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                         >  2:1054549/1‑93 (MQ=255)

                                                                 tGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                         >  2:6802352/1‑93 (MQ=255)

                                                                  gCAACTGGCAGGTCCTGGA‑G‑TTTTTGC‑C‑‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                        <  1:8767379/93‑1 (MQ=255)

                                                                   cAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCa                       <  2:5684098/93‑1 (MQ=255)

                                                                    aaCTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCat                      <  1:3635534/93‑1 (MQ=255)

                                                                     aCTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                     <  2:1333500/93‑1 (MQ=255)

                                                                     aCTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                     >  2:4450793/1‑93 (MQ=255)

                                                                       tGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                   >  1:6201473/1‑93 (MQ=255)

                                                                        ggCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTg                  <  1:5026568/93‑1 (MQ=255)

                                                                        ggCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTg                  <  1:7693417/93‑1 (MQ=255)

                                                                         gCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGa                 >  2:2103002/1‑93 (MQ=255)

                                                                           aGGTCCTGGA‑G‑TTTTTG‑‑GCCCC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGGCAATCATACTGAt                >  2:2931149/1‑93 (MQ=255)

                                                                           aGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATa               <  1:6857099/93‑1 (MQ=255)

                                                                            ggTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAc              >  2:6336910/1‑93 (MQ=255)

                                                                               ccTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAg           >  1:3956854/1‑93 (MQ=255)

                                                                                cTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc          <  2:5491846/93‑1 (MQ=255)

                                                                                cTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATAATATTGATACCAGc          >  2:4266126/1‑93 (MQ=255)

                                                                                 tGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCg         >  2:2392007/1‑93 (MQ=255)

                                                                                  ggA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa        >  2:9378375/1‑93 (MQ=255)

                                                                                  ggA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa        >  1:1132029/1‑93 (MQ=255)

                                                                                  ggA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa        <  1:5092354/93‑1 (MQ=255)

                                                                                   gA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAc       >  1:435762/1‑93 (MQ=255)

                                                                                    a‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACt      >  1:79047/1‑93 (MQ=255)

                                                                                          tttG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa  <  1:1929494/93‑1 (MQ=255)

                                                                                          tttG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa  <  2:420024/93‑1 (MQ=255)

TGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑CC‑CCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTA  >  NC_000913/1472241‑1472420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`