BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,033	(C)_5→6	coding (1149/7152 nt)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,028	1	.	C	97.9%	766.6 / 6.4	243	coding (1144/7152 nt)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/5); new base C (150/88); total (150/93)
Fisher's exact test for biased strand distribution p-value = 7.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 274 total aligned reads displayed.

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑‑c                                                                                        <  2:6018825/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑‑c                                                                                        <  2:6411868/93‑1 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑‑cccc                                                                                     >  1:3454715/1‑93 (MQ=255)

        cAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑‑cccccctt                                                                                 <  2:8493009/93‑4 (MQ=255)

           tattatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTccc                                                                               >  2:5953903/1‑93 (MQ=255)

            attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCg                                                                              >  1:8018800/1‑93 (MQ=255)

             ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGc                                                                             <  1:4692975/93‑1 (MQ=255)

             ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGc                                                                             >  2:7604790/1‑93 (MQ=255)

              tatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCt                                                                            <  1:2702425/93‑1 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCtg                                                                           >  1:7546623/1‑93 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCtg                                                                           >  2:3832667/1‑93 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCtg                                                                           <  2:6061445/93‑1 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCtg                                                                           >  2:2555313/1‑93 (MQ=255)

                tGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGcgca                                                                          >  2:1899407/1‑90 (MQ=255)

                    gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGAc                                                                      >  1:3409746/1‑93 (MQ=255)

                     aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGAcc                                                                     >  2:8821872/1‑93 (MQ=255)

                     aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGAcc                                                                     >  2:4019686/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCt                                                                    >  1:8126346/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCt                                                                    <  2:1226145/93‑1 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCt                                                                    >  2:3380740/1‑93 (MQ=255)

                       gAAGTGGCCTTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCtt                                                                   <  1:8840237/93‑1 (MQ=255)

                       gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCtt                                                                   <  2:5629303/93‑1 (MQ=255)

                       gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCtt                                                                   <  1:4013565/93‑1 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTc                                                                  <  1:151030/93‑1 (MQ=255)

                         aGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCa                                                                 <  1:6826385/93‑1 (MQ=255)

                          gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCCTTCCCGCTGATGACCTTCa                                                                 >  1:1784721/1‑93 (MQ=255)

                          gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAg                                                                <  2:3940444/93‑1 (MQ=255)

                           tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAgc                                                               >  1:3093928/1‑93 (MQ=255)

                           tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAgc                                                               <  2:2270996/93‑1 (MQ=255)

                            ggCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAgcg                                                              >  2:3175722/1‑93 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAgcgc                                                             >  2:2756769/1‑93 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAgcgc                                                             >  2:5152001/1‑93 (MQ=255)

                               ccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAGCCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCgg                                                           <  1:4783976/93‑1 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:9919357/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:5597785/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:4582429/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  1:2453890/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:8178652/1‑93 (MQ=255)

                                   ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCa                                                       >  2:1649355/1‑93 (MQ=255)

                                    tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCaa                                                      <  1:2191623/93‑1 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  2:5229114/1‑93 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  2:7134928/1‑93 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  2:1188053/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   <  1:3302053/93‑1 (MQ=255)

                                        tAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAcccc                                                  >  2:7785243/1‑93 (MQ=255)

                                        tAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGcc                                                  >  1:7882591/1‑93 (MQ=255)

                                        aaaggACGGTCGGCAAATGAATCCTCATGCTATAACCGCTGGACTTAACTA‑T‑A‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCa                                                 <  1:6429113/89‑1 (MQ=255)

                                         aaaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCa                                                 <  1:7849229/93‑1 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                                >  2:9837636/1‑93 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                                >  2:5342677/1‑93 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                                >  1:6003182/1‑93 (MQ=255)

                                           aGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAgg                                               <  1:6564987/93‑1 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                              >  1:4924895/1‑93 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                              >  1:9061809/1‑93 (MQ=255)

                                              cGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTa                                            >  1:1061175/1‑93 (MQ=255)

                                                aTCGGTAAAGTACTCCTGATGCCATAACCGCTGGACTTACCTA‑TGC‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaaa                                          <  2:7632119/93‑1 (MQ=255)

                                                aTCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaaa                                          >  1:202340/1‑93 (MQ=255)

                                                aTCGGCAAAGTAATCCTCATGCCATAACCGATGGACTTAACTA‑T‑ACCCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaa                                           >  1:9461284/1‑93 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                        >  2:3083739/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       >  2:4979851/1‑93 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                      <  1:2811029/93‑1 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                      <  2:3914386/93‑1 (MQ=255)

                                                     cAATGTAACACTCATGCCATAACCGCTGGACTTAACTATT‑C‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAggg                                     <  1:4748153/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑TCC‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    <  2:5433159/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  2:428710/1‑93 (MQ=255)

                                                       aaGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCg                                   >  2:4427927/1‑93 (MQ=255)

                                                        aGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGa                                  >  1:8182057/1‑93 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                >  1:1903750/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:10025079/93‑1 (MQ=255)

                                                            aTCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAt                              >  1:9738245/1‑93 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATg                             >  1:7118995/1‑93 (MQ=255)

                                                              ccTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGa                            <  2:6277928/93‑1 (MQ=255)

                                                               cTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGac                           >  2:5823102/1‑93 (MQ=255)

                                                               cTCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGac                           >  2:6823809/1‑93 (MQ=255)

                                                                tCATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGaca                          <  2:4208409/93‑1 (MQ=255)

                                                                 cATGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGacac                         <  1:5287175/93‑1 (MQ=255)

                                                                  aTGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAcc                        >  2:9198915/1‑93 (MQ=255)

                                                                   tGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAccg                       >  1:5742196/1‑92 (MQ=255)

                                                                   tGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAccc                       >  2:8261248/1‑93 (MQ=255)

                                                                   tGCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAccc                       >  2:6463068/1‑93 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCg                      >  2:6613359/1‑93 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCg                      >  1:7244599/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  1:7316757/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGTAAATGACACCCGtttt                  >  1:1282138/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  >  2:10014548/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCCCTGATGACCTTCAGCGCGGAGCAAAGCCAGGGGAAAAAGGGCGAAAAAGACACCCGtttt                  >  2:2998070/1‑93 (MQ=255)

                                                                         aaCCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTg                 <  1:4347447/93‑1 (MQ=255)

                                                                          aCCGCTGGACTTAACTA‑TCC‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc                <  2:848295/93‑1 (MQ=255)

                                                                          aCCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc                >  2:6912627/1‑93 (MQ=255)

                                                                          aCCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc                >  2:9057491/1‑93 (MQ=255)

                                                                          aCCGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc                >  1:463343/1‑93 (MQ=255)

                                                                           ccGCTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGcc               >  1:864710/1‑93 (MQ=255)

                                                                              cTGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAGAATGACACCCGTTTTGCCGTc            >  1:9931284/1‑93 (MQ=255)

                                                                               tGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCg           >  1:2048421/1‑93 (MQ=255)

                                                                               tGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCg           >  1:5046821/1‑93 (MQ=255)

                                                                               tGGACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCg           <  2:5690624/93‑1 (MQ=255)

                                                                                 gACTTAACTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAt         >  2:893409/1‑93 (MQ=255)

                                                                                  aCTTAACTA‑T‑ACCCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAt         <  2:7550952/93‑1 (MQ=255)

                                                                                       aCTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacc   >  1:8338174/1‑93 (MQ=255)

                                                                                        cTA‑T‑AC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacct  >  2:972854/1‑93 (MQ=255)

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`