BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	300,243	+G	coding (869/957 nt)	yagS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	300,240	1	.	G	99.2%	807.3 / ‑2.0	254	coding (872/957 nt)	yagS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (157/95); total (159/95)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 270 total aligned reads displayed.

CAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTATGTG  >  NC_000913/300149‑300327

cAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑g                                                                                        <  1:2543706/93‑1 (MQ=255)

 aaaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑gg                                                                                       >  2:6167979/1‑93 (MQ=255)

  aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggg                                                                                      >  1:9226860/1‑93 (MQ=255)

  aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggg                                                                                      <  2:5175786/93‑1 (MQ=255)

  aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggg                                                                                      <  2:6533214/93‑1 (MQ=255)

  aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTGTTCAGCGGTGGGA‑T‑ggg                                                                                      >  1:8977554/1‑93 (MQ=255)

   aTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑gggg                                                                                     <  1:381934/93‑2 (MQ=255)

   aTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑gggg                                                                                     <  1:2491320/93‑2 (MQ=255)

    tttCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggggc                                                                                    >  2:5394699/1‑91 (MQ=255)

     ttCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggggcg                                                                                   <  2:8178016/93‑4 (MQ=255)

      tCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggggcgc                                                                                  <  1:8346423/93‑5 (MQ=255)

         tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTgg                                                                                <  2:5313451/93‑1 (MQ=255)

         tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTgg                                                                                <  1:7261527/93‑1 (MQ=255)

         tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTgg                                                                                >  2:4842166/1‑93 (MQ=255)

         tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTgg                                                                                >  1:4656506/1‑93 (MQ=255)

          gCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTggg                                                                               >  2:3183968/1‑92 (MQ=255)

            cTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGa                                                                             >  2:4993609/1‑93 (MQ=255)

               tgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACa                                                                          <  2:7966871/93‑1 (MQ=255)

                 cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGc                                                                        <  1:711370/93‑1 (MQ=255)

                      gCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCATCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTc                                                                   <  2:7622588/93‑1 (MQ=255)

                      gCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTc                                                                   >  2:3643754/1‑93 (MQ=255)

                      gCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTc                                                                   >  2:1055255/1‑93 (MQ=255)

                       cTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCa                                                                  >  1:413830/1‑93 (MQ=255)

                       cTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCa                                                                  <  1:9447837/93‑1 (MQ=255)

                         tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                >  1:4537935/1‑93 (MQ=255)

                         tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                >  2:4064281/1‑93 (MQ=255)

                         tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                <  1:1304031/93‑1 (MQ=255)

                           aGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCAtata                                                              <  2:7758987/93‑1 (MQ=255)

                           aGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCAtata                                                              <  2:8636301/93‑1 (MQ=255)

                            gCCAGTACGGAGTCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             <  1:852078/93‑1 (MQ=255)

                               aGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGc                                                          >  1:600887/1‑93 (MQ=255)

                               aGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGc                                                          >  2:3322911/1‑93 (MQ=255)

                                gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         >  2:1943417/1‑93 (MQ=255)

                                gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         <  2:2007916/93‑1 (MQ=255)

                                gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         <  1:8649322/93‑1 (MQ=255)

                                gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGATTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         >  2:5053140/1‑93 (MQ=255)

                                 tACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCt                                                        >  2:8410592/1‑93 (MQ=255)

                                   cGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgc                                                      >  2:6833470/1‑93 (MQ=255)

                                    ggAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcg                                                     <  1:6932407/93‑1 (MQ=255)

                                     gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    >  1:8181768/1‑93 (MQ=255)

                                     gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    >  1:5096686/1‑93 (MQ=255)

                                     gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    <  2:3513789/93‑1 (MQ=255)

                                      aGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGcc                                                   >  2:5717777/1‑93 (MQ=255)

                                       ggCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccc                                                  >  1:7361281/1‑93 (MQ=255)

                                       ggCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccc                                                  >  2:6720882/1‑93 (MQ=255)

                                          aaGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCt                                               >  1:6668874/1‑93 (MQ=255)

                                           aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              <  1:5779905/93‑1 (MQ=255)

                                           aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              <  1:1477805/93‑1 (MQ=255)

                                           aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              >  2:583371/1‑93 (MQ=255)

                                           aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              <  2:7502490/93‑1 (MQ=255)

                                           aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              >  2:1210496/1‑93 (MQ=255)

                                             cGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTggg                                            <  1:8243234/93‑1 (MQ=255)

                                             cGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTggg                                            >  2:6506351/1‑93 (MQ=255)

                                              gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           <  2:2675574/93‑1 (MQ=255)

                                                  gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       <  1:8068651/93‑1 (MQ=255)

                                                  gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  2:2356519/1‑93 (MQ=255)

                                                  gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  2:8336264/1‑93 (MQ=255)

                                                  gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  1:7028160/1‑93 (MQ=255)

                                                  gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  2:7236374/1‑93 (MQ=255)

                                                  gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  1:2410884/1‑93 (MQ=255)

                                                    ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  2:9521121/1‑93 (MQ=255)

                                                    ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCCGGGATAGCTg                                     >  2:5842647/1‑93 (MQ=255)

                                                     tcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGa                                    <  1:1820844/93‑1 (MQ=255)

                                                     tcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGa                                    >  1:7609365/1‑93 (MQ=255)

                                                       gcCACCAGGAGTTTAAGGGTGTTTACAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAACAGAGc                                  <  2:8924273/93‑1 (MQ=255)

                                                       gcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTATCATATACGGCCTGCGCCCCCTGGGATAGCTGAGc                                  <  1:8942933/93‑1 (MQ=255)

                                                        ccAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCa                                 >  1:7682703/1‑93 (MQ=255)

                                                        ccAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCa                                 <  2:4509863/93‑1 (MQ=255)

                                                         cAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCAt                                <  1:7947528/93‑1 (MQ=255)

                                                           aCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATcc                              >  1:5213224/1‑93 (MQ=255)

                                                             aGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            <  1:5430934/93‑1 (MQ=255)

                                                             aGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            <  2:8684086/93‑1 (MQ=255)

                                                              ggAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCa                           <  2:5298386/93‑1 (MQ=255)

                                                              ggAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCa                           >  1:10013545/1‑93 (MQ=255)

                                                              ggAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCa                           <  1:9499866/93‑1 (MQ=255)

                                                               gAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAg                          >  2:9417459/1‑93 (MQ=255)

                                                                  tttAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCACTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTAGGATAGCTGAGCATCCGCAACCt                       >  2:3202571/1‑93 (MQ=255)

                                                                   ttAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATCGCTGAGCATCCGCAGCCTc                      >  2:2249699/1‑93 (MQ=255)

                                                                   ttAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTc                      >  2:102173/1‑93 (MQ=255)

                                                                    tAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCa                     <  1:3012043/93‑1 (MQ=255)

                                                                     aaaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    <  1:3873042/93‑1 (MQ=255)

                                                                     aaaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    <  2:1775121/93‑1 (MQ=255)

                                                                     aaaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    <  1:2804437/93‑1 (MQ=255)

                                                                     aaaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    <  1:3398159/93‑1 (MQ=255)

                                                                      aaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGTTAGCTGAGCATCCGCAGCCTCAAt                   >  2:9232652/1‑93 (MQ=255)

                                                                      aaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAAt                   >  2:1789003/1‑93 (MQ=255)

                                                                      aaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAAt                   <  1:7484483/93‑1 (MQ=255)

                                                                      aaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAAt                   >  1:7975456/1‑93 (MQ=255)

                                                                        ggTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgc                 >  1:6330437/1‑93 (MQ=255)

                                                                         gtgtTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgcg                <  1:9913937/93‑1 (MQ=255)

                                                                          tgtTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgcgc               <  1:320985/93‑1 (MQ=255)

                                                                          ggtTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgcgc               <  1:31717/92‑1 (MQ=255)

                                                                           gtTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGcc              <  1:1011154/93‑1 (MQ=255)

                                                                            ttttCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCa             >  1:4494588/1‑93 (MQ=255)

                                                                             tttCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAg            <  1:5398898/93‑1 (MQ=255)

                                                                               tCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAggg          >  1:9651289/1‑93 (MQ=255)

                                                                                cAGCGGTGGGAGG‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGAATACCTGCGCATCCGCAGCCTCAATGCGCCAGGGc         >  2:9694760/1‑93 (MQ=255)

                                                                                 aGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCt        >  2:345031/1‑93 (MQ=255)

                                                                                      tGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgt   >  2:5999382/1‑93 (MQ=255)

                                                                                       gggA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgtg  <  2:4280468/93‑1 (MQ=255)

CAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTATGTG  >  NC_000913/300149‑300327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`