BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,563,585	(C)_5→6	coding (1320/1404 nt)	b2451 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,563,580	1	.	C	95.8%	727.5 / 22.4	237	coding (1325/1404 nt)	b2451	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (5/5); new base C (114/112); total (119/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 275 total aligned reads displayed.

GCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑CC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA  >  NC_000913/2563488‑2563667

gCGAATACCTCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑t                                                                                            <  2:6797719/93‑1 (MQ=255)

  gAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc                                                                                         >  1:4457146/1‑93 (MQ=255)

  gAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc                                                                                         >  2:5751794/1‑93 (MQ=255)

    aTACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc                                                                                      >  2:5538174/1‑93 (MQ=255)

     tACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑c                                                                                    >  2:8680384/1‑93 (MQ=255)

     tACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑c                                                                                    <  1:6299928/93‑1 (MQ=255)

     tACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑c                                                                                    >  1:9217235/1‑93 (MQ=255)

       ccGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccg                                                                                  >  2:3581277/1‑91 (MQ=255)

       ccGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccg                                                                                  >  2:841259/1‑91 (MQ=255)

       ccGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccg                                                                                  >  1:5457559/1‑91 (MQ=255)

         gCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccgcg                                                                                <  1:2420818/93‑5 (MQ=255)

         gCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccgcg                                                                                >  2:3200243/1‑89 (MQ=255)

         gCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccgcg                                                                                >  1:3934650/1‑89 (MQ=255)

         gCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑cc‑cc‑ccgcg                                                                                >  1:4597183/1‑89 (MQ=255)

           aTTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑cgcgcg                                                                               >  1:525269/1‑93 (MQ=255)

            ttCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCgg                                                                              >  2:1780469/1‑93 (MQ=255)

            ttCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCgg                                                                              >  1:8884256/1‑93 (MQ=255)

              cAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGAc                                                                            <  2:3804814/93‑1 (MQ=255)

              cAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGAc                                                                            <  2:4218371/93‑1 (MQ=255)

              cAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑CC‑CCACGCGCGGAc                                                                            >  1:1169425/1‑93 (MQ=255)

               aggaaggaAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACa                                                                           >  1:3182132/1‑93 (MQ=255)

               aggaaggaAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACa                                                                           <  2:10045638/93‑1 (MQ=255)

               aggaaggaAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACa                                                                           >  2:2917577/1‑93 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGTCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAAt                                                                         <  2:5788778/93‑1 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCCAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑CCTCC‑CGCGCGGaacat                                                                         >  2:9604723/1‑89 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAAt                                                                         >  1:8659483/1‑93 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAAt                                                                         >  2:5146731/1‑93 (MQ=255)

                   aggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCa                                                                       <  1:6899934/93‑1 (MQ=255)

                    ggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCAc                                                                      >  2:372821/1‑93 (MQ=255)

                      gaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACtt                                                                    <  1:6662230/92‑1 (MQ=255)

                      aaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACtt                                                                    <  2:5892660/93‑1 (MQ=255)

                      aaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACtt                                                                    <  1:4316999/93‑1 (MQ=255)

                      aaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACtt                                                                    <  1:5930137/93‑1 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTc                                                                   <  1:8834874/93‑1 (MQ=255)

                           cGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCa                                                               <  1:7359837/93‑1 (MQ=255)

                            gAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCaa                                                              >  2:8750623/1‑93 (MQ=255)

                            gAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACCTCGTCaa                                                              <  1:9793606/93‑1 (MQ=255)

                             aGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAAt                                                             >  1:2962666/1‑93 (MQ=255)

                              gTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATg                                                            <  1:4702315/93‑1 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGa                                                           <  2:2212357/93‑1 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGa                                                           <  2:7610837/93‑1 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGa                                                           >  1:7506846/1‑93 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGa                                                           <  2:3128972/93‑1 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGACAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGa                                                           <  1:7944529/93‑1 (MQ=255)

                                 aTTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACt                                                         >  2:8267756/1‑93 (MQ=255)

                                 aTTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACt                                                         >  1:6153011/1‑93 (MQ=255)

                                  tttCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACtc                                                        >  1:8274950/1‑92 (MQ=255)

                                   ttCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGc                                                       >  1:7036612/1‑93 (MQ=255)

                                       ccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAAc                                                   <  2:2413412/93‑1 (MQ=255)

                                       ccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAAc                                                   <  1:6680511/93‑1 (MQ=255)

                                        cgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACg                                                  >  2:6858779/1‑93 (MQ=255)

                                        cgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACg                                                  <  1:8450283/93‑1 (MQ=255)

                                          tcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCTATTACTGCCAACggg                                                >  2:8957966/1‑93 (MQ=255)

                                          tcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACggg                                                <  1:7678516/93‑1 (MQ=255)

                                           caccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGa                                               <  2:4458460/93‑1 (MQ=255)

                                           caccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGa                                               <  2:8830059/93‑1 (MQ=255)

                                             ccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             <  2:6404580/93‑1 (MQ=255)

                                             ccgACACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAAG‑C‑CC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             <  1:1415207/93‑1 (MQ=255)

                                               ggCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgttg                                           >  1:1913948/1‑93 (MQ=255)

                                                gCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AGCC‑CC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgttgt                                          <  2:1562070/93‑1 (MQ=255)

                                                     aCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAg                                     >  2:399635/1‑93 (MQ=255)

                                                      ccGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGc                                    >  2:9797774/1‑93 (MQ=255)

                                                      ccGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGc                                    <  1:1098818/93‑1 (MQ=255)

                                                      ccGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGc                                    <  2:3638080/93‑1 (MQ=255)

                                                       cGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCt                                   >  2:9287157/1‑93 (MQ=255)

                                                         aTCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgt                                 <  2:6105266/93‑1 (MQ=255)

                                                         aTCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgt                                 >  1:1790571/1‑93 (MQ=255)

                                                          tCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgtg                                >  2:5811641/1‑93 (MQ=255)

                                                          tCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgtg                                >  1:8560505/1‑93 (MQ=255)

                                                          tCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgtg                                <  1:9548793/93‑1 (MQ=255)

                                                           ccGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTgg                               >  2:1956003/1‑93 (MQ=255)

                                                            cGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTggg                              <  1:3407824/93‑1 (MQ=255)

                                                             gCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgc                             <  2:4586864/93‑1 (MQ=255)

                                                               cAAAAAGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgcgc                           >  1:3528044/1‑93 (MQ=255)

                                                                aaaaaGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGca                          >  1:2353415/1‑93 (MQ=255)

                                                                 aaaaGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaa                         <  1:3779777/93‑1 (MQ=255)

                                                                 aaaaGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaa                         <  1:6595648/93‑1 (MQ=255)

                                                                  aaaGAGGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaac                        <  1:2535229/93‑1 (MQ=255)

                                                                    agagGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaacaa                      >  1:4351688/1‑93 (MQ=255)

                                                                    agagGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaacaa                      >  1:9424934/1‑93 (MQ=255)

                                                                     gagGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCaacaac                     >  1:7290005/1‑93 (MQ=255)

                                                                      agGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAacaaca                    <  1:6439983/93‑1 (MQ=255)

                                                                      agGCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAacaaca                    <  2:6255715/93‑1 (MQ=255)

                                                                       ggCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACAt                   >  2:8162694/1‑93 (MQ=255)

                                                                        gCGTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATg                  <  1:4991052/93‑1 (MQ=255)

                                                                          gTACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGcc                <  1:5762960/93‑1 (MQ=255)

                                                                           tACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGccc               <  1:2069431/93‑1 (MQ=255)

                                                                           tACCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGCACAATCGCATCGTCAATGACTGCCAACGGGATTTGTTGTAGCTGTGGGCGCAACAACATGccc               <  2:4823283/93‑1 (MQ=255)

                                                                            aCCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCa              <  2:6985475/93‑1 (MQ=255)

                                                                            aCCAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCa              <  2:9191541/93‑1 (MQ=255)

                                                                              cAATGTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            <  1:1635289/93‑1 (MQ=255)

                                                                                aTGCCGATAT‑AGCC‑CC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGc          <  1:2632334/93‑1 (MQ=255)

                                                                                  gTCGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCtt        >  1:7705491/1‑93 (MQ=255)

                                                                                    cGATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTa      <  1:8751269/93‑1 (MQ=255)

                                                                                     gATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAc     >  2:802073/1‑93 (MQ=255)

                                                                                     gATAT‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAc     <  2:1696984/93‑1 (MQ=255)

                                                                                      atat‑AG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAcc    <  2:3381141/93‑1 (MQ=255)

                                                                                       tat‑aG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCACCAACATCCCCAGAGCTTTACCa   >  1:2285449/1‑93 (MQ=255)

                                                                                       tat‑aG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCa   >  1:918462/1‑93 (MQ=255)

                                                                                        at‑aG‑TCCC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCaa  >  2:2590020/1‑93 (MQ=255)

GCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATAT‑AG‑T‑CC‑CC‑CGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA  >  NC_000913/2563488‑2563667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`