BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,729,373	+C	intergenic (‑195/+247)	rrsG ← / ← clpB	16S ribosomal RNA/ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,729,371	1	.	C	99.7%	900.4 / NA	293	intergenic (‑193/+249)	rrsG/clpB	16S ribosomal RNA/ClpB
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (135/157); minor base A (1/0); total (136/157)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

TCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTT‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTT  >  NC_000913/2729281‑2729458

tcaaGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTT‑cc                                                                                       <  2:7804951/90‑1 (MQ=1)

                          aaaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                                                              <  2:4067089/93‑1 (MQ=0)

                            aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                            <  2:5352221/93‑1 (MQ=9)

                            aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                            >  1:4747621/1‑93 (MQ=9)

                            aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                            <  2:8461453/93‑1 (MQ=12)

                             ttGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                                                           <  2:6684518/93‑1 (MQ=16)

                              tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                          <  2:8484431/93‑1 (MQ=16)

                              tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                          <  2:6278291/93‑1 (MQ=16)

                              tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                          >  2:5192019/1‑93 (MQ=16)

                               gTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                                         <  1:555/93‑1 (MQ=255)

                               gTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                                         >  2:6001761/1‑93 (MQ=255)

                               gTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                                         <  1:4570810/93‑1 (MQ=255)

                                ttAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                                                        <  1:5941897/93‑1 (MQ=255)

                                ttAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                                                        >  1:5675553/1‑93 (MQ=255)

                                ttAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGAATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                                                        <  1:1395990/93‑1 (MQ=255)

                                 tAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                                       <  2:5542072/93‑1 (MQ=255)

                                  aaaGATCAGTGCCTCCTCCCTTTTTCTCAGCCGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTCCTTTTctct                                                      <  1:10014289/93‑1 (MQ=11)

                                   aaGAACAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg                                                     <  1:8903275/93‑1 (MQ=255)

                                     gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                   <  1:9478737/93‑1 (MQ=255)

                                     gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                   >  1:2906148/1‑93 (MQ=255)

                                       gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                                 <  2:7227505/93‑1 (MQ=255)

                                       gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                                 >  2:863713/1‑93 (MQ=255)

                                       gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                                 <  1:9930727/93‑1 (MQ=255)

                                       gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                                 <  1:1256178/93‑1 (MQ=255)

                                         aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                               <  1:8338274/93‑1 (MQ=255)

                                         aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                               <  1:3365440/93‑1 (MQ=255)

                                         aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                               <  1:7128629/93‑1 (MQ=255)

                                          gTGCCGCTTCGGTTTTTCTCAGCGGCGCGGGGTGTGCATAATACCCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt                                              <  2:9552977/93‑1 (MQ=17)

                                          gTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt                                              <  1:3090777/93‑1 (MQ=255)

                                           tGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                             >  2:6567929/1‑93 (MQ=255)

                                           tGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                             >  2:3580436/1‑93 (MQ=255)

                                           tGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                             >  1:5359321/1‑93 (MQ=255)

                                           tGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                             >  1:4316722/1‑93 (MQ=255)

                                           tGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                             >  1:2166746/1‑93 (MQ=255)

                                             ccGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct                                           >  1:4913424/1‑93 (MQ=255)

                                             ccGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct                                           <  2:9320065/93‑1 (MQ=255)

                                              cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                          <  2:3663300/93‑1 (MQ=255)

                                              cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                          >  2:6275248/1‑93 (MQ=255)

                                              cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                          <  2:9767564/93‑1 (MQ=255)

                                               gcttcgcttTTTCTCAGCGGCGCGGGGTGTGTATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:561129/1‑93 (MQ=255)

                                               gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         <  1:626087/93‑1 (MQ=255)

                                               gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  2:5417895/1‑93 (MQ=255)

                                               gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         <  1:73531/93‑1 (MQ=255)

                                               gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:373480/1‑93 (MQ=255)

                                               ccttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         <  1:885918/92‑1 (MQ=255)

                                                 ttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg                                       <  1:1136651/93‑1 (MQ=255)

                                                 ttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg                                       >  2:5878630/1‑93 (MQ=255)

                                                  tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                      <  1:8560518/93‑1 (MQ=255)

                                                  tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                      <  2:6991874/93‑1 (MQ=255)

                                                   cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                     <  2:4421585/93‑1 (MQ=255)

                                                   cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                     >  2:9901268/1‑93 (MQ=255)

                                                    gcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga                                    <  1:1636363/93‑1 (MQ=255)

                                                     cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                   <  1:6568825/93‑1 (MQ=255)

                                                     cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                   <  1:1091731/93‑1 (MQ=255)

                                                     cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                   >  1:9738260/1‑93 (MQ=255)

                                                      tttttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc                                  <  2:8821579/93‑1 (MQ=255)

                                                      tttttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc                                  >  2:7550719/1‑93 (MQ=255)

                                                       ttttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc                                 >  2:1114492/1‑93 (MQ=255)

                                                       ttttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc                                 >  1:7271360/1‑93 (MQ=255)

                                                        tttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                                >  1:6938897/1‑93 (MQ=255)

                                                        tttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                                >  1:7822009/1‑93 (MQ=255)

                                                        tttCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                                <  2:508128/93‑1 (MQ=255)

                                                         tcctcAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcccc                               <  2:3123040/91‑1 (MQ=255)

                                                            tcAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                            <  1:5850848/93‑1 (MQ=255)

                                                               gcggcgCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                         >  2:5773514/1‑93 (MQ=255)

                                                                cggcgCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                        <  1:6435180/93‑1 (MQ=255)

                                                                 ggcgCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAccc                       <  2:5237721/93‑1 (MQ=255)

                                                                 ggcgCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAccc                       <  2:795474/93‑1 (MQ=255)

                                                                 ggcgCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAccc                       >  2:2923186/1‑93 (MQ=255)

                                                                   cgcgGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcgg                     <  2:8228884/93‑1 (MQ=255)

                                                                     cgGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcg                   <  1:2002356/93‑1 (MQ=255)

                                                                     cgGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcg                   <  1:531053/93‑1 (MQ=255)

                                                                     cgGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcg                   >  2:9641236/1‑93 (MQ=255)

                                                                      ggggTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                  >  1:9260997/1‑93 (MQ=255)

                                                                      ggggTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                  <  2:6178612/93‑1 (MQ=255)

                                                                       gggTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc                 >  1:9089628/1‑93 (MQ=255)

                                                                       gggTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc                 >  2:273253/1‑93 (MQ=255)

                                                                        ggTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGgcggcg                >  2:6131183/1‑93 (MQ=255)

                                                                         gtgtGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgt               <  2:7067806/93‑1 (MQ=255)

                                                                         gtgtGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgt               >  2:5934790/1‑93 (MQ=255)

                                                                          tgtgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtg              >  2:4316300/1‑93 (MQ=255)

                                                                          tgtgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtg              >  1:3881348/1‑93 (MQ=255)

                                                                           gtgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtgt             <  2:3388464/93‑1 (MQ=255)

                                                                            tgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt            <  1:1025731/93‑1 (MQ=255)

                                                                            tgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt            <  1:7629386/93‑1 (MQ=255)

                                                                            tgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt            <  2:1306617/93‑1 (MQ=255)

                                                                            tgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt            >  2:165287/1‑93 (MQ=255)

                                                                            tgCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt            >  2:2094412/1‑93 (MQ=255)

                                                                             gCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGttt           >  2:806223/1‑93 (MQ=255)

                                                                             gCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGttt           >  2:2537582/1‑93 (MQ=255)

                                                                              cATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTg          <  1:7149702/93‑1 (MQ=255)

                                                                              cATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTg          >  1:2350605/1‑93 (MQ=255)

                                                                               ataataCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGc         >  1:8048373/1‑93 (MQ=255)

                                                                                 aataCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCg       >  2:7366329/1‑93 (MQ=255)

                                                                                 aataCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCg       >  1:5207942/1‑93 (MQ=255)

                                                                                  ataCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCTCCGACCCGGCGGCGTGTTTGCCgt      <  1:1792861/93‑1 (MQ=255)

                                                                                  ataCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgt      <  1:8215050/93‑1 (MQ=255)

                                                                                   taCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgtt     <  1:7001476/93‑1 (MQ=255)

                                                                                   taCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgtt     <  2:1148355/93‑1 (MQ=255)

                                                                                      gCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgttgtt  <  2:8315217/93‑1 (MQ=255)

TCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTT‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTT  >  NC_000913/2729281‑2729458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`