BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,676	+C	coding (535/570 nt)	ygfQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,675	1	.	C	99.7%	962.7 / ‑6.5	297	L178F (TTG→TTC)	ygfQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (143/152); minor base T (1/0); total (144/153)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762

gCTCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTg                                                                                          >  2:291463/1‑93 (MQ=255)

gCTCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAAAGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTg                                                                                          >  1:4126215/1‑93 (MQ=255)

  tCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cc                                                                                       >  2:4188221/1‑92 (MQ=255)

  tCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cc                                                                                       >  2:2117541/1‑92 (MQ=255)

   cACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cca                                                                                      <  1:6952355/93‑3 (MQ=255)

   cACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cca                                                                                      <  1:8647672/93‑3 (MQ=255)

    aCGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                     <  1:1289435/93‑4 (MQ=255)

    aCGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                     <  1:9555976/93‑4 (MQ=255)

       gTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   >  2:1231865/1‑93 (MQ=255)

       gTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   <  1:5989182/93‑1 (MQ=255)

       gTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   <  1:4397656/93‑1 (MQ=255)

       gTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   <  1:7534045/93‑1 (MQ=255)

         aCAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGgcg                                                                                 <  2:5880302/93‑1 (MQ=255)

          cAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGgcgc                                                                                >  1:8526876/1‑93 (MQ=255)

           aGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCt                                                                               <  2:1638717/93‑1 (MQ=255)

            ggtatcggcatcGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTc                                                                              <  2:3431864/93‑1 (MQ=255)

            ggtatcggcatcGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTc                                                                              <  1:7200969/93‑1 (MQ=255)

            ggtatcggcatcGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTc                                                                              >  1:5627529/1‑93 (MQ=255)

            ggtatcggcatcGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTCATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTc                                                                              >  1:2698369/1‑93 (MQ=255)

             gtatcggcatcgGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCg                                                                             <  2:7348626/93‑1 (MQ=255)

              tatcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCgg                                                                            <  2:3552516/93‑1 (MQ=255)

              tatcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCgg                                                                            <  1:1976312/93‑1 (MQ=255)

              tatcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCgg                                                                            <  1:5167146/93‑1 (MQ=255)

                tcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTg                                                                          >  2:7142181/1‑93 (MQ=255)

                tcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTg                                                                          <  1:3534075/93‑1 (MQ=255)

                  ggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCg                                                                        <  1:7277948/90‑1 (MQ=255)

                    gatcggTCTGTTTCTGCTTCTGATTGCTGCTCGCGGTGTGGGTATGGTTATCAAAAACCGGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGtt                                                                      <  2:1697811/92‑1 (MQ=255)

                    gatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGtt                                                                      <  2:7890851/92‑1 (MQ=255)

                    catcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGtt                                                                      >  2:4107291/2‑93 (MQ=255)

                      tcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTa                                                                    >  2:5617144/1‑93 (MQ=255)

                       cggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAc                                                                   >  1:6856348/1‑93 (MQ=255)

                        gggCTGTTTGTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                  >  1:656595/1‑93 (MQ=255)

                        gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                  >  1:3421642/1‑93 (MQ=255)

                         ggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcct                                                                 >  1:644220/1‑93 (MQ=255)

                          gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                                >  2:74999/1‑93 (MQ=255)

                          gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                                >  1:8872993/1‑93 (MQ=255)

                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                               <  2:2979356/93‑1 (MQ=255)

                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                               >  1:5677552/1‑93 (MQ=255)

                            tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGTCAGTGGCGCTCGGTGCGTTTAcctcct                                                              >  1:5034118/1‑93 (MQ=255)

                            tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcct                                                              <  1:8303795/93‑1 (MQ=255)

                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             >  2:8827043/1‑93 (MQ=255)

                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             >  2:2819756/1‑93 (MQ=255)

                              tttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTc                                                            >  1:1368757/1‑93 (MQ=255)

                               ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                           >  2:5359897/1‑93 (MQ=255)

                                tCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTccc                                                          >  2:4939662/1‑93 (MQ=255)

                                tCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTccc                                                          <  2:2765159/93‑1 (MQ=255)

                                tCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTccc                                                          <  2:9457531/93‑1 (MQ=255)

                                  tgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCgg                                                        >  2:7677377/1‑93 (MQ=255)

                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     <  2:8076136/93‑1 (MQ=255)

                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     <  2:2344570/93‑1 (MQ=255)

                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGCGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     <  2:9329580/93‑1 (MQ=255)

                                        tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                  <  1:54674/93‑1 (MQ=255)

                                           ttGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgatga                                               >  1:5804923/1‑93 (MQ=255)

                                            tgctgcTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAg                                              >  1:8206709/1‑93 (MQ=255)

                                              ctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCt                                            <  1:5836663/93‑1 (MQ=255)

                                                gctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTg                                          >  2:1305845/1‑93 (MQ=255)

                                                 ctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGc                                         <  1:1749779/93‑1 (MQ=255)

                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        <  2:449716/93‑1 (MQ=255)

                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        >  2:8695730/1‑93 (MQ=255)

                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        >  2:1866425/1‑93 (MQ=255)

                                                   aaCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTg                                       <  1:2955023/93‑1 (MQ=255)

                                                   aaCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTg                                       <  1:4742990/93‑1 (MQ=255)

                                                    aCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTgg                                      <  1:5335403/93‑1 (MQ=255)

                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   >  1:1101686/1‑93 (MQ=255)

                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   >  1:2323890/1‑93 (MQ=255)

                                                         gtgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTg                                 <  2:5233354/93‑1 (MQ=255)

                                                         gtgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGGTTACTTCCTTCCCGGTGAAGATGAGCTTGCTGGGGCTg                                 >  2:8972536/1‑93 (MQ=255)

                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                <  2:6397896/93‑1 (MQ=255)

                                                            ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCg                              <  1:6139858/93‑1 (MQ=255)

                                                            ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCg                              >  2:4613139/1‑93 (MQ=255)

                                                            ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCg                              >  2:2399094/1‑93 (MQ=255)

                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            >  1:2735979/1‑93 (MQ=255)

                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            >  2:2606735/1‑93 (MQ=255)

                                                                tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                          <  1:32326/93‑1 (MQ=255)

                                                                tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                          <  1:9060999/93‑1 (MQ=255)

                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         <  2:2001773/93‑1 (MQ=255)

                                                                    tATCAAAAACGCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:836338/93‑1 (MQ=255)

                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  1:1456850/93‑1 (MQ=255)

                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:2443443/93‑1 (MQ=255)

                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:8492469/93‑1 (MQ=255)

                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:9137329/93‑1 (MQ=255)

                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCAGTGCGTTTGCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  1:984170/93‑1 (MQ=255)

                                                                     aTCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTc                     <  1:9769973/93‑1 (MQ=255)

                                                                     aTCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTc                     >  2:6676073/1‑93 (MQ=255)

                                                                      tCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCg                    <  1:390938/93‑1 (MQ=255)

                                                                        aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGc                  >  1:7513850/1‑93 (MQ=255)

                                                                         aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                 >  1:280488/1‑93 (MQ=255)

                                                                          aaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCt                >  2:9753374/1‑93 (MQ=255)

                                                                           aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               <  2:3045706/93‑1 (MQ=255)

                                                                              ccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGgag            >  1:2435744/1‑93 (MQ=255)

                                                                              ccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTCGGGCTGGCGGTCATCTTCGGCCTGgag            >  2:6317437/1‑93 (MQ=255)

                                                                                gATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGaa          >  2:6876820/1‑93 (MQ=255)

                                                                                gATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGaa          >  1:2065608/1‑93 (MQ=255)

                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         <  2:1103588/93‑1 (MQ=255)

                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         <  2:7991583/93‑1 (MQ=255)

                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         <  1:6253989/93‑1 (MQ=255)

                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         >  1:446411/1‑93 (MQ=255)

                                                                                       ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  1:7892401/93‑1 (MQ=255)

                                                                                       ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   >  1:3673939/1‑93 (MQ=255)

                                                                                        gCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgcg  >  1:926780/1‑93 (MQ=255)

GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`