BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,525	(C)_6→7	coding (402/630 nt)	yqiE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,519	1	.	C	95.7%	871.9 / 27.7	281	coding (408/630 nt)	yqiE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/8); new base C (140/129); total (144/137)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 333 total aligned reads displayed.

CGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175427‑3175606

cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑g                                                                                           >  2:4447786/1‑93 (MQ=255)

  aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cc                                                                                       <  1:8284515/93‑1 (MQ=255)

  aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cc                                                                                       >  1:4836388/1‑93 (MQ=255)

  aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cc                                                                                       >  2:2487275/1‑93 (MQ=255)

  aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cc                                                                                       >  2:9649027/1‑93 (MQ=255)

    tatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cccc                                                                                     >  1:2238126/1‑93 (MQ=255)

      ttttCGTTTTCATCACCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cccccc                                                                                   <  1:2933063/91‑1 (MQ=255)

      tCTTCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cccccc                                                                                   <  2:5848067/93‑1 (MQ=255)

        ttCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cccccccg                                                                                 >  2:4970709/1‑91 (MQ=255)

        ttCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑cccccccg                                                                                 <  2:4709072/93‑3 (MQ=255)

          ttttttCATCAGCCAGCCCGTGACTCCCGCTTGCGGTCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑CCCCCCcggg                                                                               <  2:5503045/91‑2 (MQ=255)

          cGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑CCCCCCcggg                                                                               >  2:4199256/1‑92 (MQ=255)

           gTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑CCCCCCcgggc                                                                              >  2:6774360/1‑91 (MQ=255)

           gTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑CCCCCCcgggc                                                                              >  2:1418194/1‑91 (MQ=255)

            ttttCATCAGCCAGCCGGTGAATCCCGCTTGGGGTCGTGGCGCCCCCTCCCCCCCCCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCt                                                                              <  2:7034785/93‑1 (MQ=255)

            ttttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCt                                                                              <  1:1143030/93‑1 (MQ=255)

            ttttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCt                                                                              >  1:9146431/1‑93 (MQ=255)

             tttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCtt                                                                             >  2:1546385/1‑93 (MQ=255)

             tttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCtt                                                                             >  1:8571913/1‑93 (MQ=255)

              ttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTg                                                                            >  2:4002506/1‑93 (MQ=255)

                catcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGcc                                                                          <  2:5340389/93‑1 (MQ=255)

                 agcagccagcCGGTGAATCCCGCTGGGGGTCGTGCCGCCCACCTCCCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCa                                                                         <  2:7250069/91‑1 (MQ=255)

                  tcaGCCAGCCCGTGAATCCCGCTTGCGGTCGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAg                                                                        <  2:1852527/93‑1 (MQ=255)

                   caGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAgg                                                                       >  1:9814941/1‑93 (MQ=255)

                     gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGaa                                                                     >  1:256541/1‑93 (MQ=255)

                      ccAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGaaa                                                                    <  2:9702843/93‑1 (MQ=255)

                       cAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAAc                                                                   <  2:2343078/93‑1 (MQ=255)

                       cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAAc                                                                   >  2:2153617/1‑93 (MQ=255)

                       cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAAc                                                                   >  1:280495/1‑93 (MQ=255)

                         gcccGTGAATACCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACtt                                                                 <  1:7101792/91‑1 (MQ=255)

                         gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACtt                                                                 >  2:7746084/1‑93 (MQ=255)

                         gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACtt                                                                 >  1:2105460/1‑93 (MQ=255)

                            cGTGAATACCGCTTGCGGTCTTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAac                                                              <  2:9460809/93‑1 (MQ=255)

                            cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GCCCCCCCCGGGCTTGCCAGGAAACTTaa                                                               <  1:2489116/93‑1 (MQ=255)

                            cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAac                                                              >  1:3337076/1‑93 (MQ=255)

                            cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAac                                                              <  1:8149659/93‑1 (MQ=255)

                             gTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAaca                                                             <  2:283700/93‑1 (MQ=255)

                             gTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAaca                                                             >  2:7951310/1‑93 (MQ=255)

                              tGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTAACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAacac                                                            >  2:3524729/1‑93 (MQ=255)

                                  tACCGCTTCCGTTCGTGGCGCCCACTCCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCATGAAACTTAACACCGGt                                                        <  1:3801435/93‑1 (MQ=255)

                                   cccGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGtt                                                       <  2:8590398/92‑1 (MQ=255)

                                    cctcTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGttt                                                      <  2:3004762/90‑1 (MQ=255)

                                    cctcTTGCGATCGTGGCGTCCACTCCACCCACCATAATAGAAGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGACAGGAAACTTAACACCGGttt                                                      <  2:8972028/90‑1 (MQ=255)

                                    ccGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGttt                                                      >  2:9164171/1‑93 (MQ=255)

                                     cGCTTGTGGGGGGGGCTTCCACTGCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  2:4093456/93‑1 (MQ=255)

                                     cGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  2:5074686/93‑1 (MQ=255)

                                     cGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     >  1:2329553/1‑93 (MQ=255)

                                      gCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                    >  1:4932529/1‑93 (MQ=255)

                                       cTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGt                                                   >  2:6322358/1‑93 (MQ=255)

                                        ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  1:9042582/1‑93 (MQ=255)

                                        ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  1:1023702/1‑93 (MQ=255)

                                         tGCGGTCGTGCCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTcc                                                 <  1:4549894/93‑1 (MQ=255)

                                          gCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCg                                                <  1:6824862/93‑1 (MQ=255)

                                            ggTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGtt                                              >  1:1742608/1‑93 (MQ=255)

                                              tCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                            >  2:852163/1‑93 (MQ=255)

                                              tCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                            >  2:7372051/1‑93 (MQ=255)

                                                ggggCCTCCCCTTCCCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAc                                          <  2:9023964/91‑1 (MQ=255)

                                                 tGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACt                                         >  2:8273284/1‑93 (MQ=255)

                                                    cGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATc                                      >  2:3272215/1‑93 (MQ=255)

                                                    cGTCCACTTCCCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATc                                      <  2:8072943/93‑1 (MQ=255)

                                                     gTCCCCTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     <  1:6369108/93‑1 (MQ=255)

                                                      tCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                    >  1:3535321/1‑93 (MQ=255)

                                                      tCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                    >  1:5973879/1‑93 (MQ=255)

                                                      tCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                    >  1:2027776/1‑93 (MQ=255)

                                                       ccACTCCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   <  1:4405434/93‑1 (MQ=255)

                                                         aCTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                 >  1:1895618/1‑93 (MQ=255)

                                                         aCTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCACTcc                                 >  1:6543711/1‑93 (MQ=255)

                                                          cTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTccc                                <  1:972745/93‑1 (MQ=255)

                                                           ttCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GCCCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTCTCAGTccc                                >  1:3193704/1‑93 (MQ=255)

                                                           ttCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAGCACCGGTTTGGTCCGTTTGACTATCAGTCCCg                               <  2:2799818/93‑1 (MQ=255)

                                                           ttCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcccc                               >  1:1480545/1‑92 (MQ=255)

                                                            tcccccACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGc                              <  1:7410131/90‑1 (MQ=255)

                                                              cccccccATAATTGACGAACGCTCCCTGTGG‑‑C‑‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGTTTTGGTCCTTTTGACTATCAGTCCCGCct                            <  2:4604898/92‑1 (MQ=255)

                                                               cccACCATAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctc                           <  1:869024/93‑1 (MQ=255)

                                                                ccaccaTAATTGACGACCGCTCACTG‑GTCCC‑‑CCCCCCGGGCTTGCCAGGAAACTTACCCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCctc                           <  2:6724830/93‑1 (MQ=255)

                                                                   ccaTAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCa                       >  1:2970102/1‑93 (MQ=255)

                                                                   ccaTAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCa                       <  2:9773776/93‑1 (MQ=255)

                                                                   ccaTAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCa                       >  2:6036083/1‑93 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                      >  2:6420216/1‑93 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                      <  1:4189672/93‑1 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                      >  1:9326341/1‑93 (MQ=255)

                                                                     ctAATTGACGAACGCTCACTG‑GTC‑C‑‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAAt                     <  1:5702407/92‑1 (MQ=255)

                                                                      tAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGCAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATc                    >  1:8252297/1‑93 (MQ=255)

                                                                      tAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATc                    >  2:5883028/1‑93 (MQ=255)

                                                                      tAATTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATc                    <  1:876018/93‑1 (MQ=255)

                                                                        aTTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCCGTCCCGCCTCTTCAATCGc                  >  1:1274310/1‑93 (MQ=255)

                                                                        aTTGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGc                  >  2:7080848/1‑93 (MQ=255)

                                                                          tGACGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  1:7990926/93‑1 (MQ=255)

                                                                            aCGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcg              >  1:5533710/1‑93 (MQ=255)

                                                                            aCGAACGCTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcg              <  1:5101585/93‑1 (MQ=255)

                                                                             ccaccGCCCACCG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTACCAGTCCCGCCTCTTCAATCGCTTcgc             <  2:4772475/89‑1 (MQ=255)

                                                                               aaCGCTCACGG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa           <  1:8616901/93‑1 (MQ=255)

                                                                                 cGCCCACTG‑GT‑‑GC‑CCCCCCGGGTTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACg         <  1:7350902/93‑1 (MQ=255)

                                                                                   cTCGCTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg       >  2:9836481/1‑93 (MQ=255)

                                                                                   cTCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg       >  1:9521770/1‑93 (MQ=255)

                                                                                    tcccTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTACCGCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc      <  1:7730784/90‑1 (MQ=255)

                                                                                    tCACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc      >  1:9426597/1‑93 (MQ=255)

                                                                                     cACTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCa     <  2:7658475/93‑1 (MQ=255)

                                                                                       cTG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCATTCCCGCCTCTTCAATCGCTTCGCGATGGCCAAc   >  1:9993381/1‑93 (MQ=255)

                                                                                        tG‑GT‑‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa  <  1:7660244/93‑1 (MQ=255)

CGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTG‑GT‑‑G‑‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175427‑3175606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`