BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,357	+G	coding (163/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,356	1	.	G	98.7%	1017.5 / 3.2	318	coding (162/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (173/141); total (175/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 362 total aligned reads displayed.

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑C‑GC‑GGA‑CG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c                                                                                       <  2:1849118/93‑1 (MQ=255)

  cTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑gg                                                                                    >  2:3070471/1‑92 (MQ=255)

  cTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑gg                                                                                    <  2:8557359/93‑2 (MQ=255)

  cTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑gg                                                                                    <  2:5111933/93‑2 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑ggc                                                                                   >  1:1130081/1‑91 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑ggc                                                                                   <  2:3738428/93‑3 (MQ=255)

   ctCATCGCTCTCCTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑ggc                                                                                   <  2:8482602/92‑3 (MQ=255)

    tCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCCAAGCCGGTG‑T‑c‑ggcg                                                                                  <  2:9921935/93‑4 (MQ=255)

    tCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑ggcg                                                                                  >  2:2688816/1‑90 (MQ=255)

     cATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑c‑ggcgg                                                                                 <  2:6892408/93‑5 (MQ=255)

                    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCt                                                                  <  1:4753496/93‑1 (MQ=255)

                     gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCtt                                                                 >  1:4448862/1‑93 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTg                                                                >  2:3433469/1‑93 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTg                                                                >  2:5487529/1‑93 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTg                                                                >  2:9516967/1‑93 (MQ=255)

                       ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCAGGA‑CGCTCGGTAGCCTTGc                                                               <  1:2024660/93‑1 (MQ=255)

                        gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGcc                                                              <  2:7014431/93‑1 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGccc                                                             <  2:6610296/93‑1 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGccc                                                             <  2:3510914/93‑1 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCt                                                            >  2:7903924/1‑93 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCt                                                            <  1:8222865/93‑1 (MQ=255)

                           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑TAG‑GCGGGA‑CGCTCGGTAGCCTTGCCCTg                                                           <  1:3822436/93‑1 (MQ=255)

                           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTg                                                           >  1:3596692/1‑93 (MQ=255)

                           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTg                                                           <  2:5676589/93‑1 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGa                                                          >  1:1677978/1‑93 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGa                                                          <  1:6386299/93‑1 (MQ=255)

                             tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGAt                                                         >  2:8971200/1‑93 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATc                                                        >  2:632439/1‑93 (MQ=255)

                               cTGTTGGATTANTGNAAGGAATGCCGCTGGATNAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCa                                                       <  2:4800906/93‑1 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCa                                                       <  1:5021046/93‑1 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCa                                                       <  1:798851/93‑1 (MQ=255)

                                tGTTGGATTAATGGAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCAt                                                      <  1:87459/93‑1 (MQ=255)

                                tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:4292641/1‑93 (MQ=255)

                                tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:124336/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:6484345/1‑93 (MQ=255)

                                  ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATgg                                                    >  2:7630926/1‑93 (MQ=255)

                                    ggATTAATGCGAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  1:6016310/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  <  2:1901903/93‑1 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  1:9176145/1‑93 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGACCC‑TCGGTCGCCTTGCCCTGATCCTgggat                                                 >  2:7239022/1‑91 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 <  1:2678239/93‑1 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               <  1:1891225/93‑1 (MQ=255)

                                         aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                             <  2:9398488/93‑1 (MQ=255)

                                         aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                             >  1:1244772/1‑93 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  1:7349373/1‑93 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  1:7777276/1‑93 (MQ=255)

                                           tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  1:8903070/1‑93 (MQ=255)

                                             cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         <  2:5935039/93‑1 (MQ=255)

                                             cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:4966572/1‑93 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGGCCTGATCATGGGTTTTGGCGCaa                                        >  2:9174620/1‑93 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  1:8563553/1‑93 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  1:2931647/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGG‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  2:7610328/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:2810869/93‑1 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:8050724/93‑1 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:1941845/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  1:7425982/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:8651238/93‑1 (MQ=255)

                                                  aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  2:2550120/93‑1 (MQ=255)

                                                  aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  1:9025884/93‑1 (MQ=255)

                                                  aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  1:4557897/93‑1 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:5943891/1‑93 (MQ=255)

                                                     gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:1349809/1‑93 (MQ=255)

                                                      ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTT‑C‑CGGCGGGA‑CGCTCCGTAGCCCTGCCCTCATCCTGGGTTTTGGCGCAATGCTGGGc                                >  1:2460835/1‑93 (MQ=255)

                                                      ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  2:8767000/93‑1 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:7641254/1‑93 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCCGGGCaaaa                            >  2:3945246/1‑93 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:9642151/93‑1 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAAt                           >  2:3926058/1‑93 (MQ=255)

                                                            gATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          >  2:2440209/1‑93 (MQ=255)

                                                            gATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          >  2:2948445/1‑93 (MQ=255)

                                                               aaaGTTATTGGCTCCATCAAAGCCGGTGTT‑G‑GCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGTTCGGCAAGATGCTg                       >  1:8274389/1‑93 (MQ=255)

                                                               aaaGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  1:4706599/1‑93 (MQ=255)

                                                                aaGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  2:454410/93‑1 (MQ=255)

                                                                aaGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:4168766/1‑93 (MQ=255)

                                                                aaGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:9381442/1‑93 (MQ=255)

                                                                 aGTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  1:1373846/93‑1 (MQ=255)

                                                                  gTTATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    <  2:668085/93‑1 (MQ=255)

                                                                   ttATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  1:7015016/1‑93 (MQ=255)

                                                                    tATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCACAATGCTGGCAGa                  >  1:1516940/1‑93 (MQ=255)

                                                                    tATTGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  <  2:6623700/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTG‑T‑CTGCGGGA‑GGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGTCAAAATGCTGGCAGAc                 <  2:9609624/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  2:8856463/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:3135707/1‑93 (MQ=255)

                                                                        ggCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:229596/93‑1 (MQ=255)

                                                                        ggCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  2:8081659/93‑1 (MQ=255)

                                                                        ggCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:2196775/93‑1 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  1:3978651/93‑1 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             >  1:6789223/1‑93 (MQ=255)

                                                                           tCCATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCggg           >  2:2217012/1‑92 (MQ=255)

                                                                           cccATCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:8222370/92‑1 (MQ=255)

                                                                              aTCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  1:9835737/1‑93 (MQ=255)

                                                                               tCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:5235587/1‑93 (MQ=255)

                                                                               tCAAAGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  2:2999282/1‑93 (MQ=255)

                                                                                 aaaGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca     >  1:9573780/1‑93 (MQ=255)

                                                                                 aaaGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca     >  1:6946691/1‑93 (MQ=255)

                                                                                  aaGCCGGTG‑T‑CTGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGACAAAATGCTGGCAGACTGCGGTGGCGcac    <  2:575575/93‑1 (MQ=255)

                                                                                   aGCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   >  1:5782566/1‑93 (MQ=255)

                                                                                    gCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:340061/93‑1 (MQ=255)

                                                                                    gCCGGTG‑T‑CGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:2106363/93‑1 (MQ=255)

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG‑T‑C‑GC‑GGA‑CG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`