BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,360	+G	coding (166/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,358	1	.	G	99.1%	1007.6 / 0.3	317	coding (164/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (172/141); total (174/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 329 total aligned reads displayed.

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑C‑GC‑G‑GA‑C‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  1:7756510/1‑93 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  1:1723171/1‑93 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  2:2986378/1‑93 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  2:6639203/1‑93 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  2:2247569/1‑93 (MQ=255)

  gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCtt                                                                 >  2:2142673/1‑93 (MQ=255)

  gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCtt                                                                 >  1:4448862/1‑93 (MQ=255)

   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTg                                                                >  2:3433469/1‑93 (MQ=255)

   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTg                                                                >  2:8728093/1‑93 (MQ=255)

   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGGGT‑CGGCGG‑GA‑A‑GCTCGGTAGCCTTg                                                                >  2:3329686/1‑93 (MQ=255)

    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGc                                                               >  2:8083189/1‑93 (MQ=255)

    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGc                                                               <  2:251590/93‑1 (MQ=255)

    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCAG‑GA‑C‑GCTCGGTAGCCTTGc                                                               <  1:2024660/93‑1 (MQ=255)

    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGACTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGc                                                               >  1:7165725/1‑93 (MQ=255)

      cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGccc                                                             <  2:6610296/93‑1 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTAG‑GCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           <  1:3822436/93‑1 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           >  2:7878001/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           >  1:3596692/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           <  2:5676841/93‑1 (MQ=255)

         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGa                                                          >  2:8035072/1‑93 (MQ=255)

          tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGAt                                                         >  2:8971200/1‑93 (MQ=255)

           gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATc                                                        >  1:8578041/1‑93 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:6651754/93‑1 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:798851/93‑1 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:2319639/93‑1 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  2:4668475/93‑1 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:5021046/93‑1 (MQ=255)

            cTGTGGGATTAATGNAAGGAATGCCGCTGGATNAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  2:4800907/93‑1 (MQ=255)

             tGTTGGATTAATGGAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      <  1:87459/93‑1 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:420990/1‑93 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  1:9456314/1‑93 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:124336/1‑93 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:2006975/1‑93 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      <  2:88426/93‑1 (MQ=255)

                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATggg                                                   >  1:2933993/1‑93 (MQ=255)

                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATggg                                                   >  1:9066944/1‑93 (MQ=255)

                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATggg                                                   <  1:478437/93‑1 (MQ=255)

                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GACC‑C‑TCGGTAGTCTTTCCCTGATCATGGGt                                                  >  2:7629193/1‑93 (MQ=255)

                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GACC‑C‑TCGGTCGCCTTGCCCTGATCCTgggat                                                 >  2:7239022/1‑91 (MQ=255)

                   aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGttt                                                <  2:5299792/93‑1 (MQ=255)

                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               <  1:1891225/93‑1 (MQ=255)

                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGgttt                                               >  1:8938397/1‑93 (MQ=255)

                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  1:7349373/1‑93 (MQ=255)

                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  2:6352859/93‑1 (MQ=255)

                         gCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                          >  1:637206/1‑93 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:4966572/1‑93 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  1:1525466/1‑93 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         <  2:5935039/93‑1 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  1:9610833/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGGCCTGATCATGGGTTTTGGCGCaa                                        >  2:9174620/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  1:3464643/93‑1 (MQ=255)

                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑CGG‑TCGGTAGCCTTGCCGTGATCATGGGTTGTGGCGCAAt                                       >  1:6953023/1‑93 (MQ=255)

                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaag                                       >  2:3597467/1‑92 (MQ=255)

                             ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:2912543/1‑93 (MQ=255)

                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  1:7425982/1‑93 (MQ=255)

                               aaTGCCGCTGGATAAAGTTGTTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  2:7432013/93‑1 (MQ=255)

                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  1:4557897/93‑1 (MQ=255)

                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    >  1:640987/1‑93 (MQ=255)

                                aTGCCGCTTGATAAATTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATTGGTTTTGGCGCAATGCTg                                   <  2:9562946/93‑1 (MQ=255)

                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  2:8633999/1‑93 (MQ=255)

                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  2:9285887/93‑1 (MQ=255)

                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:5943890/1‑93 (MQ=255)

                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  2:1450636/1‑93 (MQ=255)

                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTTC‑CGGCGG‑GA‑C‑GCTCCGTAGCCCTGCCCTCATCCTGGGTTTTGGCGCAATGCTGGGc                                >  1:2460835/1‑93 (MQ=255)

                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                >  1:8355596/1‑93 (MQ=255)

                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  2:947837/93‑1 (MQ=255)

                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:7641254/1‑93 (MQ=255)

                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:2642059/1‑93 (MQ=255)

                                      cTGTATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             <  1:2843697/93‑1 (MQ=255)

                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAAt                           >  2:3926058/1‑93 (MQ=255)

                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGGAGCCTTGCCCTGATCATGGGCTTTGGCGCAATACTGGGCAAAAt                           >  1:8243782/1‑93 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  1:9684231/1‑93 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  1:1192418/93‑1 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  1:6910535/1‑93 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:2646725/1‑93 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:4514132/1‑93 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:4168766/1‑93 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  2:6759319/93‑1 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:3911895/1‑93 (MQ=255)

                                              aGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  1:1373846/93‑1 (MQ=255)

                                               gTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    <  2:668085/93‑1 (MQ=255)

                                                ttATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  2:1880445/1‑93 (MQ=255)

                                                ttATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   <  1:6007005/93‑1 (MQ=255)

                                                ttATTGGCTCCATCAAAGCCGGTGT‑CGGC‑GTGA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGATTTGGCGTAATGCTGGGCAAAATGCTGGCAg                   >  2:5518271/1‑93 (MQ=255)

                                                 tATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  <  1:8112150/93‑1 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:1272632/1‑93 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:3135707/1‑93 (MQ=255)

                                                    tGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  2:7088012/1‑93 (MQ=255)

                                                     ggCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:2200740/93‑1 (MQ=255)

                                                      gCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  2:3938379/93‑1 (MQ=255)

                                                      gCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  2:1926344/93‑1 (MQ=255)

                                                          cATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  2:8482999/1‑93 (MQ=255)

                                                          cATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  1:8048091/1‑93 (MQ=255)

                                                          cATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  2:9763588/93‑1 (MQ=255)

                                                           aTCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  1:9835737/1‑93 (MQ=255)

                                                            tCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  2:5760960/1‑93 (MQ=255)

                                                            tCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:5235587/1‑93 (MQ=255)

                                                            tCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:4723820/1‑93 (MQ=255)

                                                                aGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   >  1:5782566/1‑93 (MQ=255)

                                                                 gCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:9176815/93‑1 (MQ=255)

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑C‑GC‑G‑GA‑C‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`