Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,573,700 +C intergenic (+403/+997) yhgN → / ← gntK hypothetical protein/gluconokinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,573,7001.C99.6% 800.4 / NA 253intergenic (+403/+997)yhgN/gntKhypothetical protein/gluconokinase 2
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base C (119/132);  minor base A (0/1);  total (120/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑G‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786
                                                                                              |                                                                                      
   aGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑gctt                                                                                      <  1:68104/93‑4 (MQ=255)
   aGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑gctt                                                                                      <  1:5001050/93‑4 (MQ=255)
    ggCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑gcttc                                                                                     >  1:5551582/1‑89 (MQ=255)
        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGccc                                                                                 <  1:1830152/93‑1 (MQ=255)
        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGATAAC‑GCTTCGccc                                                                                 >  1:101136/1‑93 (MQ=255)
          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAg                                                                               <  1:4435068/93‑1 (MQ=255)
          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCAAGCCGGTTAAC‑GCTTCGCCCAg                                                                               <  2:1259695/93‑1 (MQ=255)
          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGAAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAg                                                                               <  2:1750316/93‑1 (MQ=255)
           gTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGt                                                                              <  2:8031596/93‑1 (MQ=255)
            tGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGTCCATGCCGGTTAAC‑GCTTCGCCCAGTg                                                                             <  1:4358036/93‑1 (MQ=255)
            gggCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTg                                                                             <  1:1770614/92‑1 (MQ=255)
              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           >  2:1045112/1‑93 (MQ=255)
              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           >  2:241120/1‑93 (MQ=255)
              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           <  2:4816974/93‑1 (MQ=255)
              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           <  2:4692246/93‑1 (MQ=255)
                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGa                                                                        <  2:381276/93‑1 (MQ=255)
                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGa                                                                        >  2:9180208/1‑93 (MQ=255)
                  aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGAc                                                                       <  2:8902311/93‑1 (MQ=255)
                  aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGAc                                                                       <  2:8372870/93‑1 (MQ=255)
                   aCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGAcc                                                                      <  1:594010/93‑1 (MQ=255)
                    cccTGAATGATGCGCACTGCGGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCt                                                                     <  1:2721937/93‑1 (MQ=255)
                     ccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCTGACCTa                                                                    >  1:2084291/1‑93 (MQ=255)
                     ccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTa                                                                    <  2:4094249/93‑1 (MQ=255)
                      cTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTAc                                                                   <  2:5896899/93‑1 (MQ=255)
                       tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACg                                                                  >  2:2613506/1‑93 (MQ=255)
                         aaTGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGcc                                                                >  1:5278270/1‑93 (MQ=255)
                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  2:6804738/1‑93 (MQ=255)
                           tgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCag                                                              >  2:3485148/1‑93 (MQ=255)
                           tgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCag                                                              <  1:8808482/93‑1 (MQ=255)
                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           <  2:3349627/93‑1 (MQ=255)
                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           >  1:3256073/1‑93 (MQ=255)
                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           >  1:7731115/1‑93 (MQ=255)
                               gcgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTc                                                          >  1:1661505/1‑93 (MQ=255)
                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                                        <  2:6528140/93‑1 (MQ=255)
                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      <  2:4507729/93‑1 (MQ=255)
                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     >  2:9752927/1‑93 (MQ=255)
                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  2:5359649/93‑1 (MQ=255)
                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  1:9787180/93‑1 (MQ=255)
                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  1:8918527/93‑1 (MQ=255)
                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     >  1:3002792/1‑93 (MQ=255)
                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     >  1:4145808/1‑93 (MQ=255)
                                     tGCAGTTGCCAGCACGAAGCAGGAGATAGCAATCGGCAAGCCCATACCGGTTAAC‑GCTTGGCCCGATCCGGGACGTACGCCAGGGTCAGCCAg                                                    >  2:7888962/1‑93 (MQ=255)
                                      gCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTAGCCCAGTGCCGGACCTACGACAGAGTCAACCAGc                                                   >  1:4217312/1‑93 (MQ=255)
                                        aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAc                                                 >  1:3769232/1‑93 (MQ=255)
                                          cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                                               <  1:3835978/93‑1 (MQ=255)
                                           tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCCGAGTCAACCAGCACCTg                                              >  2:2182189/1‑93 (MQ=255)
                                           tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                                              >  1:6090866/1‑93 (MQ=255)
                                           tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                                              <  2:1982212/93‑1 (MQ=255)
                                            gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGt                                             <  1:8193798/93‑1 (MQ=255)
                                            gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGt                                             <  2:762372/93‑1 (MQ=255)
                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGtt                                            <  1:3701327/93‑1 (MQ=255)
                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGGCCAGTGCCGGACCTACTCCAGAGACAACCAACACCTGtt                                            >  2:2400574/1‑93 (MQ=255)
                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:4366499/1‑93 (MQ=255)
                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:9540524/1‑93 (MQ=255)
                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:1899024/1‑93 (MQ=255)
                                                  aCGAATCATGTGATAGAAAACGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTAAGCCAGAGTCAACCAGCACCTGTTTGAac                                       <  1:8101939/93‑1 (MQ=255)
                                                  aCGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                                       >  2:1172724/1‑93 (MQ=255)
                                                   cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:6685614/93‑1 (MQ=255)
                                                   cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:6993929/93‑1 (MQ=255)
                                                   cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACACCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:7050706/93‑1 (MQ=255)
                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTTCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  2:189015/93‑1 (MQ=255)
                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  1:6847919/93‑1 (MQ=255)
                                                    caaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  1:7800418/92‑1 (MQ=255)
                                                     aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                                    <  1:5894030/93‑1 (MQ=255)
                                                       gCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgcc                                  <  1:1258882/93‑1 (MQ=255)
                                                         aGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc                                >  1:5369952/1‑93 (MQ=255)
                                                          ggTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc                               >  2:572029/1‑93 (MQ=255)
                                                          ggTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc                               >  1:596816/1‑93 (MQ=255)
                                                           gTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCCGTGCCGGACCTACGCCAGAGGCAACCAGCACCTGTTTGCACACGccgccc                              >  1:5177453/1‑93 (MQ=255)
                                                             gATAGCAATCGGCAGGCCCATGCCGGTTAACGG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTTGACACGccgcccgc                            >  2:2489192/1‑93 (MQ=255)
                                                             gATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc                            >  2:7105051/1‑93 (MQ=255)
                                                              aTAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCa                           >  1:9487777/1‑93 (MQ=255)
                                                              aTAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCa                           <  1:4743177/93‑1 (MQ=255)
                                                                 gCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCa                        >  1:8528780/1‑93 (MQ=255)
                                                                   aaTCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAt                      <  2:8408080/93‑1 (MQ=255)
                                                                     tCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    <  2:3969216/93‑1 (MQ=255)
                                                                     tCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    >  1:2557640/1‑93 (MQ=255)
                                                                     tCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    >  2:1544817/1‑93 (MQ=255)
                                                                        gCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACca                 <  2:2274858/93‑1 (MQ=255)
                                                                        gCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCAGTTTGAACACGCCGCCCGCACCAATCACca                 >  2:3026739/1‑93 (MQ=255)
                                                                         cAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcag                >  2:4337704/1‑93 (MQ=255)
                                                                          aGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCTACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagc               >  2:4183312/1‑93 (MQ=255)
                                                                          aGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagc               <  2:9346090/93‑1 (MQ=255)
                                                                            gCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagcag             >  2:3114410/1‑93 (MQ=255)
                                                                             cccATGCCGGTTATC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGa            <  2:689333/93‑1 (MQ=255)
                                                                              ccATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACTCGCACCTGTTTGAACACGACGCCCGCACCAATCACCAGCAGaa           >  2:1366954/1‑93 (MQ=255)
                                                                              ccATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           <  1:5685722/93‑1 (MQ=255)
                                                                                 tGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGa        >  2:6665292/1‑93 (MQ=255)
                                                                                 tGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGa        <  1:1058278/93‑1 (MQ=255)
                                                                                  gCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       >  1:6402918/1‑93 (MQ=255)
                                                                                    cGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcc     <  2:6216104/93‑1 (MQ=255)
                                                                                    cGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcc     <  2:8594899/93‑1 (MQ=255)
                                                                                     ggTTAAC‑NCTTCGCCCAGTGCNCGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  1:6321896/93‑1 (MQ=255)
                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    >  2:7410615/1‑93 (MQ=255)
                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    >  1:4105210/1‑93 (MQ=255)
                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  2:294271/93‑1 (MQ=255)
                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  1:9648436/93‑1 (MQ=255)
                                                                                      gTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   >  1:260103/1‑93 (MQ=255)
                                                                                       ttAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCccg  <  1:9572566/93‑1 (MQ=255)
                                                                                       ttAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCccg  >  1:9095207/1‑93 (MQ=255)
                                                                                              |                                                                                      
AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑G‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑