BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,775,779	A→G	I252V (ATT→GTT)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,775,779	0	A	G	99.3%	958.4 / ‑3.4	299	I252V (ATT→GTT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (147/149); total (147/152)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 309 total aligned reads displayed.

GAGACGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACATTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTGGGG  >  NC_000913/3775689‑3775868

     gTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTAtctc                                                                                    >  1:2377133/1‑93 (MQ=255)

       ggCCTGCGGTCGTGGTTGCGGGCGGCTTGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  <  1:6923077/93‑1 (MQ=255)

       ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  >  1:9221079/1‑93 (MQ=255)

       ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  <  2:4952896/93‑1 (MQ=255)

        gCCTGCTGTCGTGGTTGCGGGCGGCTAGTTTGCCAGCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTc                                                                                 <  2:5985427/93‑1 (MQ=255)

        gCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTc                                                                                 <  2:8021829/93‑1 (MQ=255)

          cTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGCAGCTGCCGGTCGTGATCTCTTCGc                                                                               >  2:8176487/1‑93 (MQ=255)

               gTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTa                                                                          <  1:8392034/93‑1 (MQ=255)

                  gTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCa                                                                       <  1:4235566/93‑1 (MQ=255)

                   tGGTTGCGGGCGGCTCGTTTGCCATCGCTCTGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      >  1:5010565/1‑93 (MQ=255)

                   tGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      <  2:6664534/93‑1 (MQ=255)

                   tGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      >  1:8656097/1‑93 (MQ=255)

                   tGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      >  1:6686764/1‑93 (MQ=255)

                    ggTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACt                                                                     <  2:2095361/93‑1 (MQ=255)

                      ttGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGc                                                                   >  2:1341675/1‑93 (MQ=255)

                        gcgggcggCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGctc                                                                 <  1:3417098/93‑1 (MQ=255)

                        gcgggcggCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGctc                                                                 <  2:1707807/93‑1 (MQ=255)

                          gggcggCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTg                                                               >  2:9491716/1‑93 (MQ=255)

                            gcggcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGcc                                                             <  1:4516987/93‑1 (MQ=255)

                            gcggcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGcc                                                             <  2:574467/93‑1 (MQ=255)

                               gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          >  2:1895905/1‑93 (MQ=255)

                               gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          <  2:2700752/93‑1 (MQ=255)

                               gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          >  2:7305838/1‑93 (MQ=255)

                               gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          >  1:1951556/1‑93 (MQ=255)

                               gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          >  2:3584610/1‑93 (MQ=255)

                                 tCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACg                                                        >  1:2547828/1‑93 (MQ=255)

                                 tCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACg                                                        <  1:7979166/93‑1 (MQ=255)

                                  cGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGc                                                       <  2:6528292/93‑1 (MQ=255)

                                   gTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCt                                                      >  1:3927910/1‑93 (MQ=255)

                                   gTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCt                                                      <  2:4083465/93‑1 (MQ=255)

                                    tttGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTg                                                     >  2:445572/1‑93 (MQ=255)

                                      tGCCATCGCTCAGTACCTTAGCTCTAACTTCCTTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGtt                                                   >  1:9759966/1‑93 (MQ=255)

                                      tGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGtt                                                   <  1:3188202/93‑1 (MQ=255)

                                       gCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTc                                                  <  2:11619/93‑1 (MQ=255)

                                        gcATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTcc                                                 <  1:1090523/92‑1 (MQ=255)

                                         cATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCt                                                >  2:9006490/1‑93 (MQ=255)

                                          aTCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTc                                               >  2:8762713/1‑93 (MQ=255)

                                             gCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCaaa                                            <  1:6486275/93‑1 (MQ=255)

                                                cAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGc                                         >  2:9251115/1‑93 (MQ=255)

                                                cAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGc                                         >  1:4446144/1‑93 (MQ=255)

                                                 aGTACCTTAGCTCTATCTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCt                                        <  2:7207710/93‑1 (MQ=255)

                                                 aGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCt                                        >  1:3314853/1‑93 (MQ=255)

                                                 aGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCt                                        <  2:3274887/93‑1 (MQ=255)

                                                 aGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCt                                        >  2:4684520/1‑93 (MQ=255)

                                                 aGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCt                                        <  1:6397244/93‑1 (MQ=255)

                                                  gTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTg                                       >  2:2321612/1‑93 (MQ=255)

                                                   tACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTgg                                      <  2:2420870/93‑1 (MQ=255)

                                                   tACCTTAGCTCAAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTgg                                      >  1:6742909/1‑93 (MQ=255)

                                                    aCCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGc                                     >  2:6775296/1‑93 (MQ=255)

                                                    aCCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGc                                     >  2:4876963/1‑93 (MQ=255)

                                                     ccTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCa                                    >  1:9168265/1‑93 (MQ=255)

                                                      cTTAGCTCTAACTTCAGTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAg                                   <  1:5301166/93‑1 (MQ=255)

                                                       ttAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGc                                  >  2:9734900/1‑93 (MQ=255)

                                                        tAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGcc                                 >  2:5423989/1‑93 (MQ=255)

                                                        tAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGcc                                 >  1:2129625/1‑93 (MQ=255)

                                                         aGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCa                                <  2:4736706/93‑1 (MQ=255)

                                                         aGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCa                                >  1:6128581/1‑93 (MQ=255)

                                                         aGCTCTAACTTCATTGGGCCGGAGCTGCCGGACATTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCa                                <  2:8815508/93‑1 (MQ=255)

                                                          gCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAg                               <  1:5610889/93‑1 (MQ=255)

                                                          gCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAg                               <  2:10035050/93‑1 (MQ=255)

                                                          gCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAg                               <  2:5263761/93‑1 (MQ=255)

                                                           ctctAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGt                              >  1:9460539/1‑93 (MQ=255)

                                                           ctctAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGt                              >  2:8530082/1‑93 (MQ=255)

                                                              tAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCg                           >  2:9901450/1‑93 (MQ=255)

                                                                aCTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCgtg                         <  1:5828400/93‑1 (MQ=255)

                                                                aCTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCgtg                         >  1:9923077/1‑93 (MQ=255)

                                                                aCTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCgtg                         >  1:4714129/1‑93 (MQ=255)

                                                                aCTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCgtg                         >  2:7599769/1‑93 (MQ=255)

                                                                aCTTCATTGGGCAGGGGCGGCCCGACGTTATCTGTTCGCTGGTATCGCTGCACTGCCTGACGCTGTGCCTCAAGCGCTGGCAGCCAGTGCgtg                         <  2:440247/93‑1 (MQ=255)

                                                                 cTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCgtgt                        <  2:9404688/93‑1 (MQ=255)

                                                                  ttCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTa                       <  1:1719098/93‑1 (MQ=255)

                                                                  ttCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTa                       >  1:5215019/1‑93 (MQ=255)

                                                                   tCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAt                      <  2:2518040/93‑1 (MQ=255)

                                                                   tCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAt                      <  2:1491460/93‑1 (MQ=255)

                                                                   tCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAt                      >  1:2191944/1‑93 (MQ=255)

                                                                    cagtGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAtt                     <  2:2234546/90‑1 (MQ=255)

                                                                    cATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAtt                     >  2:3963398/1‑93 (MQ=255)

                                                                    cATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAtt                     >  2:8120622/1‑93 (MQ=255)

                                                                     aTTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTc                    <  1:3795093/93‑1 (MQ=255)

                                                                     aTTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTc                    <  1:748993/93‑1 (MQ=255)

                                                                      ttGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTcc                   <  1:1401497/93‑1 (MQ=255)

                                                                        gggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGt                 >  1:2672521/1‑93 (MQ=255)

                                                                         ggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGtt                <  1:784827/93‑1 (MQ=255)

                                                                         ggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGtt                >  2:2963403/1‑93 (MQ=255)

                                                                          gCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGttt               <  2:4183370/93‑1 (MQ=255)

                                                                            cGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTg             >  2:6959138/1‑93 (MQ=255)

                                                                            cGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTg             <  1:4350397/93‑1 (MQ=255)

                                                                              gAGCTGCCGGGCGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGt           <  1:3148060/93‑1 (MQ=255)

                                                                              gAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGt           >  2:6246941/1‑93 (MQ=255)

                                                                                gCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTgt         >  2:5623938/1‑92 (MQ=255)

                                                                                  tGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGAtt       <  1:8917432/93‑1 (MQ=255)

                                                                                  tGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGAtt       >  2:9581660/1‑93 (MQ=255)

                                                                                  tGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGAtt       <  1:7220513/93‑1 (MQ=255)

                                                                                   gCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGAttt      <  1:3065800/93‑1 (MQ=255)

                                                                                   gCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGAttt      >  2:3745884/1‑93 (MQ=255)

                                                                                    ccGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTg     <  1:8218879/93‑1 (MQ=255)

                                                                                     cGGACGTTATCTCTTCGCTGGTACCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgg    <  2:5531797/93‑1 (MQ=255)

                                                                                      ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg   <  1:1918937/93‑1 (MQ=255)

                                                                                      ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGGGTATTCCGTTTTGGTGATTTggg   >  1:2763007/1‑93 (MQ=255)

                                                                                       gACGTTATCTCTTCGCTGGTATCCCTGCTCTGCATGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgggg  <  1:9134892/93‑1 (MQ=255)

GAGACGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACATTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTGGGG  >  NC_000913/3775689‑3775868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`