BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,776,340	G→A	G439S (GGT→AGT)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,776,340	0	G	A	99.2%	1420.4 / ‑3.6	386	G439S (GGT→AGT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (205/178); total (207/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 394 total aligned reads displayed.

ATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCC  >  NC_000913/3776250‑3776429

 tCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTc                                                                                        >  2:345655/1‑93 (MQ=255)

 tCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTc                                                                                        >  1:9749007/1‑93 (MQ=255)

 tCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTc                                                                                        >  2:5457029/1‑93 (MQ=255)

 tCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTc                                                                                        >  2:5822229/1‑93 (MQ=255)

  cTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCa                                                                                       <  1:5581534/93‑1 (MQ=255)

  cTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTGCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCa                                                                                       <  2:4789974/93‑1 (MQ=255)

   tACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCAt                                                                                      >  1:8375621/1‑93 (MQ=255)

   tACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCAt                                                                                      >  2:6686251/1‑93 (MQ=255)

      tCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCAGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCa                                                                                   <  1:624889/93‑1 (MQ=255)

       ccATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCAt                                                                                  <  1:2861101/93‑1 (MQ=255)

       ccATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCAt                                                                                  <  2:7654629/93‑1 (MQ=255)

         aTCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTc                                                                                >  2:3248835/1‑93 (MQ=255)

          tCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTcc                                                                               >  1:4024782/1‑92 (MQ=255)

            ggTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCAcc                                                                             >  1:8687805/1‑93 (MQ=255)

             gTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACct                                                                            <  2:8649698/93‑1 (MQ=255)

              tATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACctt                                                                           >  2:9172651/1‑93 (MQ=255)

              tATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACctt                                                                           >  1:4417424/1‑93 (MQ=255)

               aTGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttc                                                                          <  2:1579824/93‑1 (MQ=255)

               aTGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttc                                                                          <  2:9240174/93‑1 (MQ=255)

               aTGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttc                                                                          >  2:7864074/1‑93 (MQ=255)

                tGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGCCTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttct                                                                         >  1:3564664/1‑93 (MQ=255)

                tGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttct                                                                         >  1:7383094/1‑93 (MQ=255)

                tGGTGCGAGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACcttct                                                                         <  1:3435030/93‑1 (MQ=255)

                 ggTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttctt                                                                        >  1:4264311/1‑93 (MQ=255)

                   tGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTtcttct                                                                      <  1:2338512/93‑1 (MQ=255)

                   tGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTtcttct                                                                      >  2:5294126/1‑93 (MQ=255)

                   tGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTtcttct                                                                      >  2:3087554/1‑93 (MQ=255)

                   tGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTtcttct                                                                      <  1:6565047/93‑1 (MQ=255)

                    gCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTtctc                                                                     <  1:8838577/93‑1 (MQ=255)

                     cTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCg                                                                    >  2:3630905/1‑93 (MQ=255)

                      tGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGc                                                                   <  2:8471280/93‑1 (MQ=255)

                        gCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCg                                                                 >  2:9398672/1‑93 (MQ=255)

                         cATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGt                                                                >  1:273622/1‑93 (MQ=255)

                          aTTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGtt                                                               >  2:6924024/1‑93 (MQ=255)

                           ttCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTc                                                              >  1:5283847/1‑93 (MQ=255)

                           ttCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTc                                                              >  1:7660886/1‑93 (MQ=255)

                           ttCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTc                                                              >  2:9568670/1‑93 (MQ=255)

                            tCGCCTTTATTTCGAACTATTCCGGACTGGCATCAGCCCTGGCGCTGGCCCTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTcc                                                             <  1:4966809/93‑1 (MQ=255)

                             cGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCt                                                            <  1:6076123/93‑1 (MQ=255)

                              gCCTTTATTTCGAACTATTCCGGACTGTCATCAAGACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTc                                                           >  1:4786168/1‑93 (MQ=255)

                              gCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTc                                                           <  1:5760632/93‑1 (MQ=255)

                              gCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTc                                                           <  1:1432671/93‑1 (MQ=255)

                                cTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCgg                                                         >  1:7005540/1‑93 (MQ=255)

                                cTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCgg                                                         >  1:7521885/1‑93 (MQ=255)

                                cTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCgg                                                         >  1:485880/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        >  2:2830965/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        <  1:9394278/93‑1 (MQ=255)

                                  ttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggct                                                       >  1:9128598/1‑93 (MQ=255)

                                  ttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggct                                                       >  2:7399682/1‑93 (MQ=255)

                                   tATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctg                                                      <  1:641920/93‑1 (MQ=255)

                                    aTTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctgg                                                     <  1:9172652/93‑1 (MQ=255)

                                     tttCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctggc                                                    >  2:2153923/1‑93 (MQ=255)

                                     tttCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctggc                                                    <  2:4969336/93‑1 (MQ=255)

                                     tttCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctggc                                                    <  2:7056278/93‑1 (MQ=255)

                                      ttCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctggct                                                   >  1:4502516/1‑93 (MQ=255)

                                        cGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGctggctgg                                                 <  2:4924830/93‑1 (MQ=255)

                                          aaCTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGATGGCTgggg                                               >  1:10005015/1‑93 (MQ=255)

                                           aCTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggggg                                              >  2:9942369/1‑93 (MQ=255)

                                           aCTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggggg                                              >  2:1300068/1‑93 (MQ=255)

                                            cTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCCTGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTgggggg                                             >  2:6810272/1‑92 (MQ=255)

                                             tATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTa                                            >  1:9487035/1‑93 (MQ=255)

                                               ttCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTAtt                                          <  2:5674413/93‑1 (MQ=255)

                                                tCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTAtgc                                         >  2:7935714/1‑91 (MQ=255)

                                                tCCGGACTGGCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTc                                         <  1:3288182/93‑1 (MQ=255)

                                                 ccGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTcc                                        <  1:1582278/93‑1 (MQ=255)

                                                 ccGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTcc                                        <  2:597152/93‑1 (MQ=255)

                                                  cGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCt                                       <  1:2990659/93‑1 (MQ=255)

                                                  cGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCt                                       >  2:8376236/1‑93 (MQ=255)

                                                  cGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCt                                       >  1:7990364/1‑93 (MQ=255)

                                                   ggACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGGATTCCTg                                      >  2:3922865/1‑93 (MQ=255)

                                                    gACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGa                                     <  2:6015382/93‑1 (MQ=255)

                                                       tGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCg                                  >  1:1289005/1‑93 (MQ=255)

                                                        gTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCgg                                 >  1:6157903/1‑93 (MQ=255)

                                                         tcatcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCggg                                <  2:8906606/93‑1 (MQ=255)

                                                         tcatcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTACCCggg                                >  1:7788683/1‑93 (MQ=255)

                                                          catcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGt                               <  2:3678699/93‑1 (MQ=255)

                                                            tcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCg                             >  1:25000/1‑93 (MQ=255)

                                                             caACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCgg                            <  2:7276889/93‑1 (MQ=255)

                                                              aaCACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGa                           <  1:8126652/93‑1 (MQ=255)

                                                               acacTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGAt                          >  2:2601417/1‑93 (MQ=255)

                                                                  cTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATAcc                       >  1:3780999/1‑93 (MQ=255)

                                                                   tGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCt                      >  2:1383337/1‑93 (MQ=255)

                                                                    ggCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTc                     <  2:9635423/93‑1 (MQ=255)

                                                                      cgcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCAt                   <  2:10003851/93‑1 (MQ=255)

                                                                      cgcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCAt                   <  1:8346179/93‑1 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATc                  >  2:4439255/1‑93 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATc                  <  2:1902595/93‑1 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATc                  <  2:4164305/93‑1 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATc                  <  1:9039301/93‑1 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATc                  >  2:629520/1‑93 (MQ=255)

                                                                           gCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAAc              <  2:8173638/93‑1 (MQ=255)

                                                                             aCTGGCGCACACCAGTCATGCATTCCCCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGc            <  1:1997542/93‑1 (MQ=255)

                                                                             aCTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGc            <  1:1928719/93‑1 (MQ=255)

                                                                             aCTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGc            >  1:5134785/1‑93 (MQ=255)

                                                                              cTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTTCTGACCGGGTCGGATACCTCATCTCACGcc           >  2:6429949/1‑93 (MQ=255)

                                                                              cTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGcc           <  1:9306828/93‑1 (MQ=255)

                                                                               tGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGccc          <  1:4713447/93‑1 (MQ=255)

                                                                                  cgcACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGt       <  1:6727618/93‑1 (MQ=255)

                                                                                     acacCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTcg    >  1:686558/1‑93 (MQ=255)

                                                                                      cacCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTcgc   >  1:1789087/1‑93 (MQ=255)

ATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCC  >  NC_000913/3776250‑3776429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`