BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	439,695	A→G	Y211H (TAT→CAT)	ispA ←	geranyltranstransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	439,695	0	A	G	99.7%	1024.4 / ‑5.4	317	Y211H (TAT→CAT)	ispA	geranyltranstransferase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (176/140); total (176/141)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 324 total aligned reads displayed.

CACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTT  >  NC_000913/439605‑439784

 aCCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCtt                                                                                        >  2:7066001/1‑93 (MQ=255)

 aCCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCACTCTGCATGCtt                                                                                        <  2:4001071/93‑1 (MQ=255)

   ccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGt                                                                                      >  2:3352555/1‑93 (MQ=255)

   ccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGt                                                                                      >  1:9987347/1‑93 (MQ=255)

   ccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGt                                                                                      >  1:5913447/1‑93 (MQ=255)

     tGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAATGCCGATGCTCTCTGCATGCTTGTCg                                                                                    >  2:679497/1‑93 (MQ=255)

          ttttCCTAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTAc                                                                               >  2:8922843/1‑93 (MQ=255)

          ttttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTAc                                                                               >  1:1755285/1‑93 (MQ=255)

            ttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCg                                                                             >  1:3724746/1‑93 (MQ=255)

              cccAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGc                                                                           >  2:8543750/1‑93 (MQ=255)

               ccAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCa                                                                          <  1:3882050/93‑1 (MQ=255)

                    gTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCa                                                                     <  2:5952370/93‑1 (MQ=255)

                     ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    >  2:5396332/1‑93 (MQ=255)

                     ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    >  1:6214600/1‑93 (MQ=255)

                       gCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacga                                                                  <  1:9572989/93‑1 (MQ=255)

                        cAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacgac                                                                 <  2:1726317/93‑1 (MQ=255)

                        cAGAATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGTCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacgac                                                                 <  1:8141315/93‑1 (MQ=255)

                         aGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacgacg                                                                >  2:5189935/1‑93 (MQ=255)

                         aGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacgacg                                                                >  1:6473184/1‑93 (MQ=255)

                          gTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacgacgc                                                               >  2:6290525/1‑92 (MQ=255)

                          gTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGt                                                               <  2:7510711/93‑1 (MQ=255)

                          gTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGt                                                               >  2:3096334/1‑93 (MQ=255)

                           tATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTc                                                              <  2:3123560/93‑1 (MQ=255)

                           tATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTc                                                              >  2:2366285/1‑93 (MQ=255)

                           tATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTc                                                              >  2:4480609/1‑93 (MQ=255)

                           tATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTc                                                              >  2:5285903/1‑93 (MQ=255)

                             tctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                            >  1:322907/1‑93 (MQ=255)

                             tctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                            >  2:3594726/1‑93 (MQ=255)

                             tctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                            >  2:1155486/1‑93 (MQ=255)

                              ctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                           <  1:729164/93‑1 (MQ=255)

                              ctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                           <  1:3825228/93‑1 (MQ=255)

                               tcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                          >  2:7581477/1‑93 (MQ=255)

                               tcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                          >  1:66088/1‑93 (MQ=255)

                               tcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                          >  2:6926956/1‑93 (MQ=255)

                                cccACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                                         >  2:7969159/1‑93 (MQ=255)

                                cccACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                                         <  2:8698631/93‑1 (MQ=255)

                                  caccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtc                                                       >  1:7818264/1‑93 (MQ=255)

                                  caccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtc                                                       >  2:2052980/1‑93 (MQ=255)

                                    ccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                                     >  2:1834968/1‑93 (MQ=255)

                                    ccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                                     >  1:1520717/1‑93 (MQ=255)

                                    ccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                                     >  1:5184052/1‑93 (MQ=255)

                                     cacaTCCAGGATGTTATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                                    <  1:8058722/93‑1 (MQ=255)

                                     cacaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCATAGCACGACGTCCTTTATCTcc                                                    >  2:6227774/1‑93 (MQ=255)

                                     cacaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                                    >  2:2228479/1‑93 (MQ=255)

                                     cacaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                                    <  2:4362101/93‑1 (MQ=255)

                                      acaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                                   <  1:4459273/93‑1 (MQ=255)

                                      acaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                                   >  2:5711326/1‑93 (MQ=255)

                                       caTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                                  >  2:3634227/1‑93 (MQ=255)

                                           cAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCt                                              >  2:4955765/1‑93 (MQ=255)

                                           cAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCt                                              >  1:2576678/1‑93 (MQ=255)

                                             ggATGTCATCCTGATCCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                            <  2:483079/93‑1 (MQ=255)

                                             ggATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                            >  2:4257545/1‑93 (MQ=255)

                                             ggATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                            >  2:2737151/1‑93 (MQ=255)

                                             ggATGTCAGCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                            <  2:8310585/93‑1 (MQ=255)

                                              gATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                           <  2:6643477/93‑1 (MQ=255)

                                              gATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                           >  1:3591550/1‑93 (MQ=255)

                                              gATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                           <  2:6015367/93‑1 (MQ=255)

                                               aTGTCATCCTGAACGTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                          <  1:7636868/93‑1 (MQ=255)

                                               aTGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                          <  2:2721265/93‑1 (MQ=255)

                                                  tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                       >  1:2107487/1‑93 (MQ=255)

                                                    aTCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCAcc                                     >  1:9307217/1‑93 (MQ=255)

                                                     tCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCa                                    >  1:1599456/1‑93 (MQ=255)

                                                     tCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCa                                    >  2:4872995/1‑93 (MQ=255)

                                                     tCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCa                                    <  1:5740474/93‑1 (MQ=255)

                                                      ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  2:1654469/93‑1 (MQ=255)

                                                      ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   >  1:8850402/1‑93 (MQ=255)

                                                      ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  2:6138142/93‑1 (MQ=255)

                                                         gAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGc                                >  2:2149378/1‑93 (MQ=255)

                                                            ccTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                             <  2:820067/93‑1 (MQ=255)

                                                            ccTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                             <  1:2832030/93‑1 (MQ=255)

                                                            ccTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                             >  2:5975766/1‑93 (MQ=255)

                                                             cTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                            >  1:5403900/1‑93 (MQ=255)

                                                             cTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                            <  2:980777/93‑1 (MQ=255)

                                                             cTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                            >  1:1881746/1‑93 (MQ=255)

                                                              tGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGACcg                           >  1:6781766/1‑93 (MQ=255)

                                                               ggAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                          <  2:6485649/93‑1 (MQ=255)

                                                               ggAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                          >  1:7421534/1‑93 (MQ=255)

                                                                  aggcaaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcgg                       <  1:6947174/93‑1 (MQ=255)

                                                                  aggcaaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcgg                       >  2:7323189/1‑93 (MQ=255)

                                                                   ggcaaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcggg                      >  1:3945120/1‑92 (MQ=255)

                                                                     caaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGgcgc                    >  2:5553674/1‑93 (MQ=255)

                                                                     caaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGgcgc                    <  1:8350871/93‑1 (MQ=255)

                                                                      aaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGcgcg                   >  1:5207057/1‑93 (MQ=255)

                                                                       aggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGa                  >  2:8449108/1‑93 (MQ=255)

                                                                       aggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGa                  <  1:2170301/93‑1 (MQ=255)

                                                                        ggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGaa                 >  2:6586829/1‑93 (MQ=255)

                                                                         gcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAAt                >  2:7743458/1‑93 (MQ=255)

                                                                          ccGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATc               >  2:7798618/1‑93 (MQ=255)

                                                                             aTGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt            <  1:3497654/93‑1 (MQ=255)

                                                                              tGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATg           >  1:4605319/1‑93 (MQ=255)

                                                                              tGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATg           >  1:6054013/1‑93 (MQ=255)

                                                                               gCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgc          <  2:714502/93‑1 (MQ=255)

                                                                                 tctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc        >  2:4645109/1‑93 (MQ=255)

                                                                                 tctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc        >  2:7630360/1‑93 (MQ=255)

                                                                                 tctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc        >  2:3802900/1‑93 (MQ=255)

                                                                                  ctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGcc       <  1:2553986/93‑1 (MQ=255)

                                                                                   tctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCg      <  2:9177745/93‑1 (MQ=255)

                                                                                      gCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGtt   <  2:1378561/93‑1 (MQ=255)

                                                                                      gCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGtt   >  1:10002664/1‑93 (MQ=255)

                                                                                       cATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGttt  >  2:9489367/1‑93 (MQ=255)

CACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTT  >  NC_000913/439605‑439784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`