BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,233	T→C	L220P (CTG→CCG)	rfaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,233	0	T	C	100.0%	1720.6 / NA	525	L220P (CTG→CCG)	rfaL	O‑antigen ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (295/230); total (295/230)

Only 100 of 529 total aligned reads displayed.

CTAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTA  >  NC_000913/3795143‑3795322

 tAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGtt                                                                                        >  1:522207/1‑93 (MQ=255)

 tAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGtt                                                                                        >  2:822733/1‑93 (MQ=255)

  aaGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTc                                                                                       <  2:4107169/93‑1 (MQ=255)

   aGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTcc                                                                                      <  1:9634244/93‑1 (MQ=255)

    aaaaaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTccc                                                                                     <  1:3218396/92‑1 (MQ=255)

      aaaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCta                                                                                   <  1:9367947/93‑1 (MQ=255)

      aaaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGAAACCTTACTCCCGTTCCCta                                                                                   <  2:5095692/93‑1 (MQ=255)

       aaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCtat                                                                                  <  2:9607755/93‑1 (MQ=255)

       aaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCtat                                                                                  <  2:3866788/93‑1 (MQ=255)

       aaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCtat                                                                                  <  1:997243/93‑1 (MQ=255)

       aaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCtat                                                                                  <  1:5318653/93‑1 (MQ=255)

          tcatcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAt                                                                               <  1:7821597/93‑1 (MQ=255)

           catcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAtt                                                                              <  1:685477/93‑1 (MQ=255)

           catcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAtt                                                                              <  2:659822/93‑1 (MQ=255)

           catcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAtt                                                                              <  1:4935673/93‑1 (MQ=255)

            atcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAttt                                                                             <  1:7482452/93‑1 (MQ=255)

            atcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAttt                                                                             >  2:3542977/1‑93 (MQ=255)

             tcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTtg                                                                            <  2:2395286/93‑1 (MQ=255)

               cTTTTTTATTTTTATTAAATAGTTGCGCTGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTtgtg                                                                          <  1:7135395/93‑1 (MQ=255)

               cTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTtgtg                                                                          >  1:5155430/1‑93 (MQ=255)

                ttttttATTTTTATTAAATAGTTGCGCTGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTgtgt                                                                         <  2:2799858/93‑1 (MQ=255)

                ttttttATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTgtgt                                                                         >  1:3062881/1‑93 (MQ=255)

                ttttttATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTgtgt                                                                         >  2:5650200/1‑93 (MQ=255)

                 tttttatttttaTTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGtt                                                                        <  1:1314662/93‑1 (MQ=255)

                 tttttatttttaTTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGtt                                                                        <  2:4500828/93‑1 (MQ=255)

                   tttatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgc                                                                      <  2:6265402/93‑1 (MQ=255)

                   tttatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgc                                                                      <  1:3963802/93‑1 (MQ=255)

                     tatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgctg                                                                    <  1:9752345/93‑1 (MQ=255)

                        ttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCAt                                                                 >  1:8719283/1‑93 (MQ=255)

                        ttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCAt                                                                 >  1:2369249/1‑93 (MQ=255)

                          ttattaAATTGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTa                                                               <  1:9172636/93‑1 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCACCCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTaa                                                              <  2:7394344/93‑1 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTaa                                                              >  1:9031170/1‑93 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTaa                                                              <  2:4825391/93‑1 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTaa                                                              >  2:6738761/1‑93 (MQ=255)

                            attaAATAGTTGCGCGGTACTTTCTGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAAt                                                             <  2:5502942/93‑1 (MQ=255)

                            attaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAAt                                                             >  1:6420486/1‑93 (MQ=255)

                             ttaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATa                                                            <  2:6435220/93‑1 (MQ=255)

                              taAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAg                                                           >  2:1922805/1‑93 (MQ=255)

                                aaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCt                                                         >  2:2584659/1‑93 (MQ=255)

                                aaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCt                                                         >  2:5919366/1‑93 (MQ=255)

                                 aTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtt                                                        >  1:2464631/1‑93 (MQ=255)

                                 aTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtt                                                        <  1:8327734/93‑1 (MQ=255)

                                  tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       <  1:4800610/93‑1 (MQ=255)

                                  tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       >  2:8032507/1‑93 (MQ=255)

                                   aGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttat                                                      >  1:7388992/1‑93 (MQ=255)

                                   aGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttat                                                      >  2:3738123/1‑93 (MQ=255)

                                     ttGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttatta                                                    <  2:1702165/93‑1 (MQ=255)

                                     ttGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttatta                                                    <  2:2671155/93‑1 (MQ=255)

                                     ttGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttatta                                                    >  1:5444038/1‑93 (MQ=255)

                                      tGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTtattat                                                   <  2:8602892/93‑1 (MQ=255)

                                      tGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTtattat                                                   >  2:6032487/1‑93 (MQ=255)

                                       gcgcGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATtata                                                  >  1:3480207/1‑93 (MQ=255)

                                       gcgcGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATtata                                                  <  1:216670/93‑1 (MQ=255)

                                       gcgcGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATtata                                                  >  1:8099354/1‑93 (MQ=255)

                                        cgcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataa                                                 >  2:7794385/1‑93 (MQ=255)

                                         gcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataat                                                >  1:6011946/1‑93 (MQ=255)

                                           ggTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTAtaataa                                              >  2:9122406/1‑93 (MQ=255)

                                            gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATaaa                                             >  1:1008546/1‑93 (MQ=255)

                                            gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATaaa                                             <  2:1134396/93‑1 (MQ=255)

                                            gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATaaa                                             <  1:6446133/93‑1 (MQ=255)

                                            gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATaaa                                             >  1:8433769/1‑93 (MQ=255)

                                             tACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAAt                                            <  2:3667979/93‑1 (MQ=255)

                                              aCTTTATGTTCTCGCGCTAACACAAGACAGAGCAACGCTACTGCCGTTCCCTATAATTTGTGTTGATGCATTAATAGCTTATTATAATAAATc                                           <  2:7719770/93‑1 (MQ=255)

                                               cTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCa                                          >  1:3310932/1‑93 (MQ=255)

                                                tttATGTTCTGGCGCTAACACAAACCAGAGCAGCCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAc                                         >  2:9294225/1‑93 (MQ=255)

                                                tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAc                                         >  1:6519357/1‑93 (MQ=255)

                                                tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAc                                         >  2:8436596/1‑93 (MQ=255)

                                                tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAc                                         >  2:2253108/1‑93 (MQ=255)

                                                 ttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAcc                                        >  2:7112706/1‑93 (MQ=255)

                                                 ttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAcc                                        >  1:3559377/1‑93 (MQ=255)

                                                 ttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAcc                                        >  2:5260767/1‑93 (MQ=255)

                                                  tATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAccc                                       >  1:1215452/1‑93 (MQ=255)

                                                  tATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAccc                                       >  1:3693550/1‑93 (MQ=255)

                                                     gTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGAATTAATAGCTTATTATAATACATCACCCAAg                                    >  1:268556/1‑93 (MQ=255)

                                                      ttCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGa                                   >  1:5727535/1‑93 (MQ=255)

                                                       tCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGaa                                  <  2:6974146/93‑1 (MQ=255)

                                                           gcgcTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTc                              >  1:4618550/1‑93 (MQ=255)

                                                            cgcTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCa                             >  1:1446378/1‑93 (MQ=255)

                                                               tAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACtt                          >  2:1417603/1‑93 (MQ=255)

                                                               tAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACtt                          <  1:5076347/93‑1 (MQ=255)

                                                                aaCACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTc                         <  1:4176431/93‑1 (MQ=255)

                                                                aaCACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTc                         >  2:6860470/1‑93 (MQ=255)

                                                                  cacaAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCct                       >  2:9449439/1‑93 (MQ=255)

                                                                          agagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGtt               >  2:9869294/1‑93 (MQ=255)

                                                                          agagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGtt               <  2:5813519/93‑1 (MQ=255)

                                                                           gagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTc              >  1:553393/1‑93 (MQ=255)

                                                                           gagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTc              >  1:6807644/1‑93 (MQ=255)

                                                                            agCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTct             <  2:2938594/93‑1 (MQ=255)

                                                                             gCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTcta            >  2:215621/1‑93 (MQ=255)

                                                                               aaCCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctact          >  1:2867958/1‑93 (MQ=255)

                                                                                aCCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctacta         >  1:1630836/1‑93 (MQ=255)

                                                                                aCCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctacta         >  2:4969931/1‑93 (MQ=255)

                                                                                  ccTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTGCTCTATTGTTCTACTAAt       <  2:4330939/93‑1 (MQ=255)

                                                                                  ccTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCAATTCCTCTATTGTTCTACTAAt       <  1:1804377/93‑1 (MQ=255)

                                                                                    tACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTg     >  1:4477564/1‑93 (MQ=255)

                                                                                    tACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTg     >  1:8589164/1‑93 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  >  1:6185692/1‑93 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  <  2:8810179/93‑1 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  <  2:9708995/93‑1 (MQ=255)

CTAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTA  >  NC_000913/3795143‑3795322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`