Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 450,780 T→C T19A (ACT→GCT)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913450,7800TC99.8% 1516.2 / NA 463T19A (ACT→GCT) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (244/218);  minor base G (1/0);  total (245/218)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 472 total aligned reads displayed.

CAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTT  >  NC_000913/450690‑450869
                                                                                          |                                                                                         
cAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAgcgc                                                                                         >  2:862880/1‑93 (MQ=255)
 aaaTGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGcc                                                                                        <  2:2692014/93‑1 (MQ=255)
  aaTGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCt                                                                                       <  1:9426849/93‑1 (MQ=255)
        gTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaat                                                                                 >  2:6769444/1‑93 (MQ=255)
         tCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaata                                                                                >  2:5752289/1‑93 (MQ=255)
         tCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaata                                                                                <  1:1931405/93‑1 (MQ=255)
         tCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaata                                                                                >  1:2839984/1‑93 (MQ=255)
           aGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaataat                                                                              >  1:3145502/1‑93 (MQ=255)
           aGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaataat                                                                              >  2:6495677/1‑93 (MQ=255)
           aGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAaataat                                                                              <  2:2069511/93‑1 (MQ=255)
            gTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATg                                                                             <  1:4543574/93‑1 (MQ=255)
            gTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATg                                                                             >  2:6118565/1‑93 (MQ=255)
            gTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATg                                                                             >  1:2368855/1‑93 (MQ=255)
             tATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGa                                                                            >  1:9936496/1‑93 (MQ=255)
              aTCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGAc                                                                           <  1:3088259/93‑1 (MQ=255)
              aTCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGAc                                                                           <  2:9523230/93‑1 (MQ=255)
               tCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACa                                                                          >  1:7910875/1‑93 (MQ=255)
                cAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACaa                                                                         <  2:2451822/93‑1 (MQ=255)
                  gTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAAcc                                                                       >  1:3363827/1‑93 (MQ=255)
                  gTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAAcc                                                                       >  2:3095273/1‑93 (MQ=255)
                   tGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCa                                                                      >  1:9046725/1‑93 (MQ=255)
                   tGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCa                                                                      >  2:3763858/1‑93 (MQ=255)
                   tGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCa                                                                      <  1:1058911/93‑1 (MQ=255)
                      aCGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATcc                                                                   >  2:833549/1‑93 (MQ=255)
                      aCGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATcc                                                                   >  1:6836586/1‑93 (MQ=255)
                       cGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATccc                                                                  <  2:3611839/93‑1 (MQ=255)
                         ttGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCaa                                                                >  1:2541369/1‑93 (MQ=255)
                         ttGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCACCCCaa                                                                >  1:3794662/1‑93 (MQ=255)
                           gCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAAc                                                              >  2:3469112/1‑93 (MQ=255)
                           gCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAAc                                                              <  2:5794844/93‑1 (MQ=255)
                             tCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACtt                                                            <  2:120697/93‑1 (MQ=255)
                                aGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTa                                                         >  2:4348374/1‑93 (MQ=255)
                                aGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTa                                                         <  2:3263656/93‑1 (MQ=255)
                                aGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTa                                                         >  1:5346424/1‑93 (MQ=255)
                                 gACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTAt                                                        >  1:659696/1‑93 (MQ=255)
                                   ccAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTg                                                      >  2:9493986/1‑93 (MQ=255)
                                    cAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGt                                                     <  2:7374648/93‑1 (MQ=255)
                                     aaTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTa                                                    <  1:4195459/93‑1 (MQ=255)
                                     aaTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTa                                                    >  1:5682050/1‑93 (MQ=255)
                                      aTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAt                                                   >  2:9896900/1‑93 (MQ=255)
                                      aTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAt                                                   <  1:797087/93‑1 (MQ=255)
                                      aTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAt                                                   >  2:4457078/1‑93 (MQ=255)
                                      aTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAACGACAACCATCCCAAACTTTTATTGTAt                                                   <  1:9169157/93‑1 (MQ=255)
                                       tcggTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAtt                                                  <  2:1771567/90‑1 (MQ=255)
                                       tCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAtt                                                  >  2:6016324/1‑93 (MQ=255)
                                       tCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAtt                                                  <  1:6674671/93‑1 (MQ=255)
                                         tGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTc                                                <  1:3699831/93‑1 (MQ=255)
                                         tGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTc                                                <  1:5921965/93‑1 (MQ=255)
                                         tGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTc                                                >  2:1931540/1‑93 (MQ=255)
                                          gTCCTTTGAGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTcc                                               <  2:6989858/93‑1 (MQ=255)
                                           tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCt                                              >  1:1693059/1‑93 (MQ=255)
                                            ccTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTg                                             >  2:7968661/1‑93 (MQ=255)
                                              tttGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAg                                           >  1:10037428/1‑93 (MQ=255)
                                              tttGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAg                                           <  2:1599057/93‑1 (MQ=255)
                                                tGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGtc                                         <  1:9675143/93‑1 (MQ=255)
                                                  ggATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGtctc                                       >  1:228983/1‑93 (MQ=255)
                                                   gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCa                                      >  1:1314708/1‑93 (MQ=255)
                                                   gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCa                                      <  1:2100595/93‑1 (MQ=255)
                                                    aTCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAt                                     >  2:5459326/1‑93 (MQ=255)
                                                     tCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAtt                                    >  1:1188244/1‑93 (MQ=255)
                                                     tCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAtt                                    <  2:7826812/93‑1 (MQ=255)
                                                      cTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAttt                                   >  2:8133411/1‑93 (MQ=255)
                                                      cTAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAttt                                   >  1:5074444/1‑93 (MQ=255)
                                                       tAACAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTa                                  >  2:1085921/1‑93 (MQ=255)
                                                        aaCAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAATCTTTTATTGTATTTCCTGAGTCTCATTTaa                                 <  1:4035633/93‑1 (MQ=255)
                                                        aaCAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTaa                                 >  1:7034186/1‑93 (MQ=255)
                                                          cAGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACg                               <  2:5362645/93‑1 (MQ=255)
                                                           aGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGa                              <  1:4808764/93‑1 (MQ=255)
                                                           aGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGa                              <  2:4928755/93‑1 (MQ=255)
                                                           aGCGCAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGa                              <  1:8832182/93‑1 (MQ=255)
                                                            gcgcAGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGAc                             >  2:5127860/1‑93 (MQ=255)
                                                                aGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGGCCTCa                         <  2:2344224/93‑1 (MQ=255)
                                                                aGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCa                         <  1:7336711/93‑1 (MQ=255)
                                                                aGAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCa                         >  1:1484033/1‑93 (MQ=255)
                                                                 gAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCaa                        >  1:8643277/1‑93 (MQ=255)
                                                                 gAATTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCaa                        <  2:9800527/93‑1 (MQ=255)
                                                                  aaTTACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAAt                       >  2:6186235/1‑93 (MQ=255)
                                                                    ttACAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTc                     >  1:6124351/1‑93 (MQ=255)
                                                                     tACAGCCACTGAGCAATACAGCGCCTGCAAATAATGAGAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTcc                    <  2:5674419/93‑1 (MQ=255)
                                                                      aCAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCa                   <  2:385794/93‑1 (MQ=255)
                                                                       cAGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCAc                  <  1:8170814/93‑1 (MQ=255)
                                                                        aGCCACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTACCTGAGTCTCATTTAACGACCTCAATTCCACg                 >  2:9252685/1‑93 (MQ=255)
                                                                          ccACGGAGCAATACAGCGCCTGCAAATAAGGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACggg               <  2:2277128/93‑1 (MQ=255)
                                                                           cACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGa              >  1:2194693/1‑93 (MQ=255)
                                                                           cACTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGa              <  2:406090/93‑1 (MQ=255)
                                                                            ccTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGAt             <  1:2609811/92‑1 (MQ=255)
                                                                            aCTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGAt             <  1:7493415/93‑1 (MQ=255)
                                                                            aCTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGAt             <  2:1528446/93‑1 (MQ=255)
                                                                             cTGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATc            <  1:5974000/93‑1 (MQ=255)
                                                                              tGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCt           >  1:4418112/1‑93 (MQ=255)
                                                                              tGAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCt           >  2:9053578/1‑93 (MQ=255)
                                                                               gAGCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAACTCCACGGGATCtg          <  1:5502607/93‑1 (MQ=255)
                                                                                aGCAATACAGGGCCGGCAAACAATGACAACCATCCCAAACTTTTATTGTATTTCCTGATTCTCATTTAACGACCTCAATTCCACGGGATCtgg         >  2:7111571/1‑93 (MQ=255)
                                                                                 gCAATACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggt        <  1:6272408/93‑1 (MQ=255)
                                                                                   aaTACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggtgg      <  1:4644519/93‑1 (MQ=255)
                                                                                   aaTACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGCCTCATTTAACGACCTCAATTCCACGGGATCtgtggg      >  2:4542563/1‑89 (MQ=255)
                                                                                    aTACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGc     >  2:8782038/1‑93 (MQ=255)
                                                                                     tACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCg    >  1:5180337/1‑93 (MQ=255)
                                                                                     tACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCg    >  1:8442667/1‑93 (MQ=255)
                                                                                     tACAGCGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCg    <  1:757519/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTT  >  NC_000913/450690‑450869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑