BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,404	A→G	F456L (TTC→CTC)	ampG ←	AmpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,404	0	A	G	99.3%	922.2 / ‑3.3	293	F456L (TTC→CTC)	ampG	AmpG
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (124/167); total (125/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

Only 100 of 320 total aligned reads displayed.

TAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCAAAGG  >  NC_000913/451315‑451493

gaGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGGAAAGAGAGGt                                                                                         <  2:7556010/92‑1 (MQ=255)

    gcCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTc                                                                                    >  2:8158136/1‑93 (MQ=255)

    gcCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGAGCGTc                                                                                    >  2:7781811/1‑93 (MQ=255)

      cAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCaa                                                                                  >  1:5035357/1‑93 (MQ=255)

       aGATAATCCAGCAAACCACCAAGCACGACGCCAGAACGGGCTACTAAACCCCCGGCTTCCAGCAGACCAGGCC‑GGAAAGAGAGGCGCGCCaaa                                                                                 >  2:2363572/1‑93 (MQ=255)

       aGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCaaa                                                                                 >  1:711237/1‑93 (MQ=255)

       aGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCaaa                                                                                 >  1:6567349/1‑93 (MQ=255)

         aTAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATc                                                                               <  2:3619261/93‑1 (MQ=255)

          tAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATcc                                                                              <  2:2253192/93‑1 (MQ=255)

          tAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATcc                                                                              <  2:8243272/93‑1 (MQ=255)

            aTCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAACTCCAg                                                                            >  1:9592186/1‑93 (MQ=255)

             tCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAgc                                                                           >  1:1189892/1‑93 (MQ=255)

               cAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAgcgc                                                                         >  1:2787094/1‑93 (MQ=255)

                  cAAACCACCAAGCACGACGCCAGCAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTc                                                                      <  2:7208468/93‑1 (MQ=255)

                  cAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTc                                                                      <  2:8180580/93‑1 (MQ=255)

                    aaCCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCa                                                                    >  2:2781163/1‑93 (MQ=255)

                      ccaccaAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATc                                                                  >  1:7972810/1‑93 (MQ=255)

                      ccaccaAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATc                                                                  >  1:5341097/1‑93 (MQ=255)

                      ccaccaAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATc                                                                  <  1:4544528/93‑1 (MQ=255)

                       caccaAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCg                                                                 >  1:6117548/1‑93 (MQ=255)

                        accaAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGt                                                                >  1:1009960/1‑93 (MQ=255)

                            aGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAAt                                                            >  2:7954166/1‑93 (MQ=255)

                            aGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAAt                                                            >  2:6136882/1‑93 (MQ=255)

                             gCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATa                                                           >  2:5968038/1‑93 (MQ=255)

                             gCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATa                                                           <  2:9660135/93‑1 (MQ=255)

                               acgacgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGc                                                         <  1:2888626/93‑1 (MQ=255)

                                cgacgCCAGAAAGGGCGACTAAACCCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCa                                                        <  1:7661670/93‑1 (MQ=255)

                                cgacgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCa                                                        <  2:6452238/93‑1 (MQ=255)

                                 gacgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAg                                                       <  1:6789747/93‑1 (MQ=255)

                                  acgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGt                                                      >  2:3727972/1‑93 (MQ=255)

                                  acgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGt                                                      >  2:3977157/1‑93 (MQ=255)

                                  acgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGGGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAga                                                      >  1:486449/1‑92 (MQ=255)

                                   cgCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGAGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTa                                                     >  2:6066531/1‑93 (MQ=255)

                                     ccAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAAc                                                   <  2:6339854/93‑1 (MQ=255)

                                     ccAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAAc                                                   >  1:4251965/1‑93 (MQ=255)

                                      cAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAAcc                                                  <  1:9153619/93‑1 (MQ=255)

                                       aGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACca                                                 >  2:6890597/1‑93 (MQ=255)

                                       aGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACca                                                 <  1:4720788/93‑1 (MQ=255)

                                       aGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAAACCAAAACGACCATCGTCAATAGCAGTAACca                                                 <  1:8940090/93‑1 (MQ=255)

                                          aaGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCacac                                              <  2:4666799/93‑1 (MQ=255)

                                           aGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACg                                             >  1:4614550/1‑93 (MQ=255)

                                           aGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACg                                             <  1:2098592/93‑1 (MQ=255)

                                                 aCTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAAc                                       >  2:5423767/1‑93 (MQ=255)

                                                 aCTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAAc                                       >  1:8546070/1‑93 (MQ=255)

                                                 aCTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAAc                                       <  2:1764330/93‑1 (MQ=255)

                                                 aCTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAAc                                       >  1:2319755/1‑93 (MQ=255)

                                                  cTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACa                                      <  1:9510971/93‑1 (MQ=255)

                                                  cTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACa                                      <  2:5773628/93‑1 (MQ=255)

                                                  cTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACa                                      <  1:2518998/93‑1 (MQ=255)

                                                   tAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAg                                     <  2:9028214/93‑1 (MQ=255)

                                                   tAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAg                                     >  2:187900/1‑93 (MQ=255)

                                                     aaaCCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGc                                   >  2:2849578/1‑93 (MQ=255)

                                                      aaCCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCt                                  <  2:8747087/93‑1 (MQ=255)

                                                      aaCCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACACGCt                                  >  1:4415224/1‑93 (MQ=255)

                                                        ccccGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGa                                <  1:7480296/93‑1 (MQ=255)

                                                        agccGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCAATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGa                                <  1:5981685/91‑1 (MQ=255)

                                                          ccGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcg                              >  2:4959233/1‑93 (MQ=255)

                                                          ccGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcg                              >  2:7265815/1‑93 (MQ=255)

                                                           cGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgc                             <  1:9902865/93‑1 (MQ=255)

                                                            gACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgcc                            <  2:2638682/93‑1 (MQ=255)

                                                            gACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgcc                            <  1:5616518/93‑1 (MQ=255)

                                                            gACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgcc                            >  1:8733745/1‑93 (MQ=255)

                                                            gACTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgcc                            >  1:8176034/1‑93 (MQ=255)

                                                             aCTTCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATTGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgccc                           >  1:930202/1‑93 (MQ=255)

                                                               ttCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgcccgc                         <  1:3547326/93‑1 (MQ=255)

                                                               ttCCAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGAcgcccgc                         <  1:898276/93‑1 (MQ=255)

                                                                 ccAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCccgccg                       >  2:4384082/1‑93 (MQ=255)

                                                                 ccAGCAGAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCccgccg                       >  2:6525076/1‑93 (MQ=255)

                                                                  cagcagAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCcgccgc                      >  2:56991/1‑93 (MQ=255)

                                                                  cagcagAGCAGGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCcgccgc                      >  1:7762126/1‑93 (MQ=255)

                                                                   agcagagcagGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCgccgcc                     >  2:4556789/1‑93 (MQ=255)

                                                                     gagagcagGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAg                   <  1:7303224/92‑1 (MQ=255)

                                                                     cagagcagGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGCCGCCCGCCGCCAg                   >  1:5250014/1‑93 (MQ=255)

                                                                     cagagcagGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAg                   <  2:3178007/93‑1 (MQ=255)

                                                                      agagcagGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAgt                  <  1:6451440/93‑1 (MQ=255)

                                                                       gagcagGCA‑GGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAgtg                 >  1:7813448/1‑93 (MQ=255)

                                                                         gcaggca‑gGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTc               <  1:1329327/93‑1 (MQ=255)

                                                                          gaggca‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTcc              <  2:9590490/92‑1 (MQ=255)

                                                                            ggca‑ggAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCcac            >  2:2992353/1‑93 (MQ=255)

                                                                             gca‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCcaca           <  2:9786951/93‑1 (MQ=255)

                                                                             gca‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCcaca           <  2:9349475/93‑1 (MQ=255)

                                                                             gca‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCcaca           <  1:5157665/93‑1 (MQ=255)

                                                                             gca‑gaAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCcaca           <  2:84565/93‑1 (MQ=255)

                                                                             gaa‑ggAAAGAGAGGTCCGTCAAACCCAGCGCGTCTATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCcaca           <  2:7504003/91‑1 (MQ=255)

                                                                              ca‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACAt          <  1:6901703/93‑1 (MQ=255)

                                                                               a‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATg         <  1:1764339/93‑1 (MQ=255)

                                                                               a‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATg         <  2:1192986/93‑1 (MQ=255)

                                                                               a‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATg         >  2:8656604/1‑93 (MQ=255)

                                                                               a‑ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATg         <  2:886341/93‑1 (MQ=255)

                                                                                 ggAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACCCGGCCAACAGTCTGCCGCCCCCCGCCAGGGTCCACATgg        >  1:8335825/1‑93 (MQ=255)

                                                                                    aaGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaa     <  2:3376530/93‑1 (MQ=255)

                                                                                    aaGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaa     >  1:3887130/1‑93 (MQ=255)

                                                                                    aaGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaa     >  1:4017982/1‑93 (MQ=255)

                                                                                     agagagGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaag    >  2:1049540/1‑92 (MQ=255)

                                                                                     agagagGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaaa    <  1:5410639/93‑1 (MQ=255)

                                                                                     agagagGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaaa    <  2:9172587/93‑1 (MQ=255)

                                                                                     agagagGCGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCaaa    >  1:6621585/1‑93 (MQ=255)

                                                                                      gagaGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCAAAg   <  2:1633418/93‑1 (MQ=255)

                                                                                      gagaGGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCAAAg   <  2:5249098/93‑1 (MQ=255)

                                                                                       agagGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCAAAgg  <  1:1604505/93‑1 (MQ=255)

TAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCA‑GGAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTGACGCCCGCCGCCAGTGTCCACATGGCAAAGG  >  NC_000913/451315‑451493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`