Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,477 T→C Y456H (TAC→CAC)  lon → ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,4770TC99.7% 1118.4 / ‑5.0 345Y456H (TAC→CAC) lonATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (192/152);  minor base A (1/0);  total (193/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

Only 100 of 376 total aligned reads displayed.

GACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATG  >  NC_000913/459387‑459566
                                                                                          |                                                                                         
gACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACcaccac                                                                                         >  2:4622846/1‑93 (MQ=255)
gACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACcaccac                                                                                         >  1:4856858/1‑93 (MQ=255)
  cAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCt                                                                                       >  1:6048704/1‑93 (MQ=255)
  cAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCt                                                                                       <  2:7366195/93‑1 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      >  2:4301387/1‑93 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      >  1:9306062/1‑93 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      >  2:6317430/1‑93 (MQ=255)
    aaaTGTCTTCTGACATGCGTGGCTACCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGCCCACCACCTgg                                                                                     <  2:3478885/93‑1 (MQ=255)
    aaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTgg                                                                                     <  1:5053565/93‑1 (MQ=255)
        gTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGt                                                                                 >  1:8877073/1‑93 (MQ=255)
         tcttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTg                                                                                >  2:8130651/1‑93 (MQ=255)
          cttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTgg                                                                               <  1:2551789/93‑1 (MQ=255)
          cttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTgg                                                                               >  2:6364786/1‑93 (MQ=255)
           ttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGa                                                                              >  2:4696232/1‑93 (MQ=255)
            tctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGAt                                                                             >  1:9867525/1‑93 (MQ=255)
             ctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGAtt                                                                            <  2:8560236/93‑1 (MQ=255)
             ctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGAtt                                                                            >  1:2092136/1‑93 (MQ=255)
              tGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTa                                                                           >  2:514742/1‑93 (MQ=255)
              tGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTa                                                                           >  1:4088442/1‑93 (MQ=255)
                aCATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCGCCTGGAAGTGGATTACg                                                                         <  1:8121815/93‑1 (MQ=255)
                 cATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGa                                                                        >  1:100143/1‑93 (MQ=255)
                 cATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGa                                                                        <  2:238051/93‑1 (MQ=255)
                  ctGCGTGGCGATGCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCGCCACCTGGAAGTGGATTACGAt                                                                       <  2:1391239/92‑1 (MQ=255)
                  aTGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAt                                                                       <  2:7822574/93‑1 (MQ=255)
                  aTGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAt                                                                       <  1:6126960/93‑1 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  1:4323241/1‑93 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  2:7617653/1‑93 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  1:7839930/1‑93 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  1:6571655/1‑93 (MQ=255)
                       tGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAg                                                                  >  1:5425064/1‑93 (MQ=255)
                          cGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGa                                                               <  1:9490314/93‑1 (MQ=255)
                           gATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGAc                                                              <  2:3149856/93‑1 (MQ=255)
                           gATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGAc                                                              >  1:6678703/1‑93 (MQ=255)
                            aTCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACg                                                             >  2:404769/1‑93 (MQ=255)
                            aTCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACg                                                             >  1:5800755/1‑93 (MQ=255)
                            aTCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACg                                                             >  1:2168574/1‑93 (MQ=255)
                               cGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGa                                                          <  1:2775651/93‑1 (MQ=255)
                                 gCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATg                                                        <  2:5293090/93‑1 (MQ=255)
                                  gctctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGt                                                       <  1:6931115/92‑1 (MQ=255)
                                   ctctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt                                                      >  2:8207678/1‑93 (MQ=255)
                                   ctctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt                                                      <  1:5248168/93‑1 (MQ=255)
                                    tctgctctgcTTGAAGTGCTGGATCCAGAGCAGCACGTAGCGTTCAGCGCCCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt                                                     <  1:7469782/93‑1 (MQ=255)
                                    tctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt                                                     <  1:7738820/93‑1 (MQ=255)
                                    tctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt                                                     >  1:6382561/1‑93 (MQ=255)
                                     tggCACTGCGTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTGCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg                                                    <  1:4349046/91‑1 (MQ=255)
                                     ctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg                                                    <  1:9671216/93‑1 (MQ=255)
                                     ctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg                                                    >  2:102559/1‑93 (MQ=255)
                                      tGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGt                                                   <  2:3832257/93‑1 (MQ=255)
                                       gCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTc                                                  <  2:6745734/93‑1 (MQ=255)
                                       gCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTc                                                  >  2:748611/1‑93 (MQ=255)
                                          cTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcgcg                                               >  1:3894659/1‑93 (MQ=255)
                                           tGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGTCCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa                                              <  1:6894362/93‑1 (MQ=255)
                                           tGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGAGACCACCAACTGGAAGTGGATTACGATCTCAGCGAAGTGATGTTTGTCGCGa                                              >  1:3603574/1‑93 (MQ=255)
                                            gCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc                                             <  2:2320152/93‑1 (MQ=255)
                                              ctGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt                                           <  1:6344668/92‑1 (MQ=255)
                                               tGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTc                                          <  2:652896/93‑1 (MQ=255)
                                               tGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTc                                          <  1:5393879/93‑1 (MQ=255)
                                                    tGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACt                                     >  2:8315653/1‑93 (MQ=255)
                                                     gCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTc                                    <  1:3786050/93‑1 (MQ=255)
                                                      cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTcc                                   >  1:1288146/1‑93 (MQ=255)
                                                      cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTcc                                   <  2:2854335/93‑1 (MQ=255)
                                                       tGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGCCGTGGAACGccc                                  >  1:7354337/1‑92 (MQ=255)
                                                       tGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCa                                  <  2:9806393/93‑1 (MQ=255)
                                                       tGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCa                                  <  2:9521733/93‑1 (MQ=255)
                                                         gATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATg                                <  1:4237851/93‑1 (MQ=255)
                                                           tcgagagCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGaa                              <  2:9195183/90‑1 (MQ=255)
                                                           tCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGaa                              >  1:1417354/1‑93 (MQ=255)
                                                           tCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGaa                              <  1:4529874/93‑1 (MQ=255)
                                                            ccAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAAc                             >  2:153650/1‑93 (MQ=255)
                                                              agagCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGCGGTGTTTGTCGCGACGTCGAACTCCATGAACAt                           >  1:4823469/1‑93 (MQ=255)
                                                                  cAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCg                       >  2:7027547/1‑93 (MQ=255)
                                                                  cAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCg                       >  2:7998526/1‑93 (MQ=255)
                                                                   aGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCgg                      >  1:2378624/1‑93 (MQ=255)
                                                                    gAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGc                     >  2:2991976/1‑93 (MQ=255)
                                                                    gAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGc                     >  2:5928619/1‑93 (MQ=255)
                                                                    gAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGc                     >  1:8281853/1‑93 (MQ=255)
                                                                    gAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGc                     >  2:806119/1‑93 (MQ=255)
                                                                       cGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCAcc                  <  1:3672211/93‑1 (MQ=255)
                                                                        gTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCg                 >  2:6178974/1‑93 (MQ=255)
                                                                         tAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCAcccc                >  2:2658209/1‑91 (MQ=255)
                                                                         tAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgc                >  1:2643954/1‑93 (MQ=255)
                                                                         tAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgc                >  1:3436943/1‑93 (MQ=255)
                                                                         tAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgc                <  2:6942836/93‑1 (MQ=255)
                                                                            cGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgctgc             >  2:2156198/1‑93 (MQ=255)
                                                                            cGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgctgc             <  2:336738/93‑1 (MQ=255)
                                                                             gTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgctgct            >  2:7069859/1‑93 (MQ=255)
                                                                              ttCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGctgctg           >  2:1613776/1‑93 (MQ=255)
                                                                               tCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTgg          >  1:869672/1‑93 (MQ=255)
                                                                                 aGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGAt        >  1:1893180/1‑93 (MQ=255)
                                                                                 aGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGAt        <  2:899908/93‑1 (MQ=255)
                                                                                 aGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGAt        <  2:6459587/93‑1 (MQ=255)
                                                                                  gCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATc       >  2:5671521/1‑93 (MQ=255)
                                                                                    gACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGt     >  1:8485823/1‑93 (MQ=255)
                                                                                    gACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGt     <  2:8901012/93‑1 (MQ=255)
                                                                                     accaccaccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTa    <  1:5532687/93‑1 (MQ=255)
                                                                                     accaccaccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTa    >  1:3212197/1‑93 (MQ=255)
                                                                                     accaccaccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTa    <  1:2015580/93‑1 (MQ=255)
                                                                                       caccacTTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATg  <  2:4420071/93‑1 (MQ=255)
                                                                                       caccacCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATg  >  1:1775413/1‑93 (MQ=255)
                                                                                       caccacCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATg  <  2:1920942/93‑1 (MQ=255)
                                                                                          |                                                                                         
GACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATG  >  NC_000913/459387‑459566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑