BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,742	T→C	A320A (GCT→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,742	0	T	C	99.5%	1236.5 / ‑5.1	384	A320A (GCT→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (218/163); minor base A (2/0); total (221/163)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 407 total aligned reads displayed.

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

 aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgct                                                                                        >  1:4151411/1‑93 (MQ=255)

 aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgct                                                                                        >  2:9131918/1‑93 (MQ=255)

  tCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGccgctg                                                                                       >  2:3419385/1‑93 (MQ=255)

  tCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGccgctg                                                                                       >  2:7322967/1‑93 (MQ=255)

     gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  2:805110/93‑1 (MQ=255)

     gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  2:7607170/93‑1 (MQ=255)

       ggcggTATCGCCCAGTCTCGTTTGACTATGCTGCTGCTGCAGCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgc                                                                                  <  2:6653754/93‑1 (MQ=255)

        gcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgcg                                                                                 >  1:6591143/1‑93 (MQ=255)

         cggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCga                                                                                <  2:9525632/93‑1 (MQ=255)

           gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgaga                                                                              <  2:6072189/93‑1 (MQ=255)

            tATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGagag                                                                             <  1:4550723/93‑1 (MQ=255)

              tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCg                                                                           >  1:2430508/1‑93 (MQ=255)

              tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCg                                                                           >  1:3826143/1‑93 (MQ=255)

                   cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCt                                                                      <  1:2884310/93‑1 (MQ=255)

                    aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCtt                                                                     >  2:435656/1‑93 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  1:4735641/93‑1 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  2:5825159/93‑1 (MQ=255)

                      tctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtct                                                                   <  2:6312365/93‑1 (MQ=255)

                       ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtctc                                                                  <  2:2226230/93‑1 (MQ=255)

                        tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 >  2:7514012/1‑93 (MQ=255)

                        tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 >  1:770122/1‑93 (MQ=255)

                            ttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgctg                                                             <  2:1191420/93‑1 (MQ=255)

                             tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            >  1:5104821/1‑93 (MQ=255)

                             tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            >  2:987273/1‑93 (MQ=255)

                              gACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGta                                                           >  2:7488741/1‑93 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          <  2:9634221/93‑1 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          >  2:5672294/1‑93 (MQ=255)

                                cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         >  2:2345943/1‑93 (MQ=255)

                                cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         <  1:6109963/93‑1 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        <  1:7515990/93‑1 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        <  1:7872702/93‑1 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        >  1:9781220/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:4434103/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:8058617/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:5721193/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:5169703/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  2:3555268/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  2:5244099/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  1:3211865/1‑93 (MQ=255)

                                      tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   <  1:9715682/93‑1 (MQ=255)

                                       gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTAt                                                  <  1:424301/93‑1 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 >  1:2054578/1‑93 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                >  1:1616287/1‑93 (MQ=255)

                                          gctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgc                                               >  2:1697507/1‑93 (MQ=255)

                                           ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgcc                                              <  1:1496571/93‑1 (MQ=255)

                                           ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgcc                                              <  2:8441646/93‑1 (MQ=255)

                                              cAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgccgca                                           <  1:8221872/93‑2 (MQ=255)

                                               aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCa                                          >  1:1101335/1‑93 (MQ=255)

                                               aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAAAAATTTATCGCCCCCa                                          >  1:7346725/1‑93 (MQ=255)

                                                aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                         >  1:2480941/1‑93 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        >  1:2612884/1‑93 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        <  1:3692275/93‑1 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATAGCCGCCAGc                                        >  1:8912216/1‑93 (MQ=255)

                                                  tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCg                                       >  2:2846069/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   <  1:6855859/93‑1 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:8688829/1‑93 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:5003860/1‑93 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:4538092/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  1:1188221/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                <  1:709503/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                <  1:6974591/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  2:3815052/1‑93 (MQ=255)

                                                          atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                               <  2:4663522/93‑1 (MQ=255)

                                                             ggCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggc                            <  1:9549866/93‑1 (MQ=255)

                                                              gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  1:7459166/1‑93 (MQ=255)

                                                              gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  2:10001066/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  1:2152512/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  2:4274433/1‑93 (MQ=255)

                                                                 aGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCt                        >  2:4139372/1‑93 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:9246666/93‑1 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:7116352/93‑1 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:9715389/93‑1 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  1:1936445/93‑1 (MQ=255)

                                                                   gTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTc                      <  1:7257895/93‑1 (MQ=255)

                                                                    ttCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                     >  2:822939/1‑93 (MQ=255)

                                                                    ttCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                     >  1:10026657/1‑93 (MQ=255)

                                                                     tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    >  1:8677565/1‑93 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:1838151/93‑1 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:3419385/93‑1 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   >  2:6946307/1‑93 (MQ=255)

                                                                       aGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCAt                  >  1:268170/1‑93 (MQ=255)

                                                                        gtgtGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATc                 >  1:4393562/1‑93 (MQ=255)

                                                                         gggggAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCGTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                <  1:9039919/90‑1 (MQ=255)

                                                                          gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCCGGCGGCTTCGCATccc               <  2:8989611/93‑1 (MQ=255)

                                                                          gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc               <  2:6808669/93‑1 (MQ=255)

                                                                          gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc               >  2:3683036/1‑93 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              >  1:5504828/1‑93 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              <  2:1457803/93‑1 (MQ=255)

                                                                            ggAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCgg             >  2:5934866/1‑93 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          >  1:5955726/1‑93 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          <  1:173860/93‑1 (MQ=255)

                                                                                tATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATc         >  1:626400/1‑93 (MQ=255)

                                                                                 aTGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa        >  2:7790899/1‑93 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       <  2:3194328/93‑1 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       >  1:1126672/1‑93 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATACCGGTATCaa       >  1:8276680/1‑93 (MQ=255)

                                                                                    gCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATGCCGGTATAAAAg     >  2:2000204/1‑93 (MQ=255)

                                                                                     ccAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc    >  1:9111268/1‑93 (MQ=255)

                                                                                      cAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg   >  2:6967537/1‑93 (MQ=255)

                                                                                       aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  >  2:6494007/1‑93 (MQ=255)

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`