Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,928,464 A→G L86P (CTT→CCT)  yieN ← putative 2‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,928,4640AG94.9% 1148.8 / 35.2 394L86P (CTT→CCT) yieNputative 2‑component regulator
Reads supporting (aligned to +/- strand):  ref base A (10/10);  new base G (214/160);  total (224/170)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

Only 100 of 410 total aligned reads displayed.

ATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTAAGCGCCGG  >  NC_000913/3928374‑3928553
                                                                                          |                                                                                         
aTCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAggg                                                                                         <  1:758099/93‑1 (MQ=255)
aTCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAggg                                                                                         >  2:6627605/1‑93 (MQ=255)
aTCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAggg                                                                                         <  1:6464315/93‑1 (MQ=255)
aTCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAggg                                                                                         <  1:3084487/93‑1 (MQ=255)
  ctcATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGgggg                                                                                       <  2:2303202/93‑1 (MQ=255)
   tcATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGa                                                                                      <  1:3160436/93‑1 (MQ=255)
      tCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCa                                                                                   >  1:8783771/1‑93 (MQ=255)
        cAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCaaa                                                                                 >  1:6273342/1‑93 (MQ=255)
         aGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGCCCaaaa                                                                                >  1:4993369/1‑93 (MQ=255)
         aGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCaaaa                                                                                <  2:585917/93‑1 (MQ=255)
          gAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCaaaaa                                                                               <  1:1217167/93‑1 (MQ=255)
          gAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCaaaaa                                                                               <  2:4155789/93‑1 (MQ=255)
            aaGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGGCCTTCATCTTTTAGCGCCTGAATAGATGGGGGACCAAAAAct                                                                             >  2:9249899/1‑93 (MQ=255)
            aaGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAAct                                                                             >  2:4961704/1‑93 (MQ=255)
             aGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAActt                                                                            >  1:7927967/1‑93 (MQ=255)
             aGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAActt                                                                            >  1:2468633/1‑93 (MQ=255)
             aGACAATTACTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAActt                                                                            <  2:8589494/93‑1 (MQ=255)
              gACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAActtc                                                                           <  2:2867660/93‑1 (MQ=255)
                cAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAActtctt                                                                         <  1:6905938/93‑1 (MQ=255)
                  aTTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTcc                                                                       >  2:3532398/1‑93 (MQ=255)
                  aTTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTcc                                                                       <  2:628861/93‑1 (MQ=255)
                  aTTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTcc                                                                       <  1:4324036/93‑1 (MQ=255)
                  aTTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGAACAAAAACTTCTTcc                                                                       >  2:1767072/1‑93 (MQ=255)
                  aTTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTcc                                                                       >  2:6992684/1‑93 (MQ=255)
                   tttCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCg                                                                      >  1:8474402/1‑93 (MQ=255)
                   tttCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCg                                                                      <  2:8067399/93‑1 (MQ=255)
                       tGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGt                                                                  <  1:425015/93‑1 (MQ=255)
                        gCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTg                                                                 >  1:1631114/1‑93 (MQ=255)
                        gCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTg                                                                 >  2:9076189/1‑93 (MQ=255)
                          ttCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGga                                                               >  1:9409933/1‑93 (MQ=255)
                          ttCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGga                                                               <  2:5563975/93‑1 (MQ=255)
                            ccGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGgaga                                                             <  2:4928326/93‑1 (MQ=255)
                             cGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGaa                                                            <  2:5486346/93‑1 (MQ=255)
                              ggCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAg                                                           <  1:2981864/93‑1 (MQ=255)
                               gCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAgg                                                          >  1:640464/1‑92 (MQ=255)
                               gCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGc                                                          >  1:5805317/1‑93 (MQ=255)
                                cAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCg                                                         <  1:586972/93‑1 (MQ=255)
                                cAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCg                                                         <  2:6446205/93‑1 (MQ=255)
                                  ggTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCggg                                                       >  2:4471673/1‑93 (MQ=255)
                                  ggTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCggg                                                       >  1:4665309/1‑93 (MQ=255)
                                   gTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCgggc                                                      >  2:4682631/1‑92 (MQ=255)
                                    tAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGtc                                                     <  2:3243052/93‑1 (MQ=255)
                                    tAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGtc                                                     >  1:6768484/1‑93 (MQ=255)
                                        cGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGtcatca                                                 >  2:4000219/1‑93 (MQ=255)
                                        cGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTATTCCCGCGTGGCGAAGCGGGtcatca                                                 >  1:324246/1‑93 (MQ=255)
                                         gCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAg                                                >  2:2554451/1‑93 (MQ=255)
                                         gCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAg                                                >  2:7829781/1‑93 (MQ=255)
                                         gCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAg                                                >  2:8485922/1‑93 (MQ=255)
                                          cTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGa                                               <  2:1178436/93‑1 (MQ=255)
                                           tGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGat                                              >  1:2077961/1‑93 (MQ=255)
                                            ggTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGata                                             >  1:10003304/1‑93 (MQ=255)
                                              ttAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATAtt                                           <  1:951266/93‑1 (MQ=255)
                                               tAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTc                                          >  1:8558511/1‑93 (MQ=255)
                                                  aCGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCaaa                                       <  2:7326272/93‑1 (MQ=255)
                                                  aCGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCaaa                                       >  2:4654598/1‑93 (MQ=255)
                                                  aCGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCaaa                                       <  1:1949832/93‑1 (MQ=255)
                                                   cGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAAc                                      >  1:2787438/1‑93 (MQ=255)
                                                      tCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAAcgcg                                   <  2:3132389/93‑1 (MQ=255)
                                                       cATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAAcgcgc                                  <  2:6675291/93‑1 (MQ=255)
                                                        aTAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAAcgcgcg                                 <  1:107088/93‑1 (MQ=255)
                                                        aTAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAAcgcgcg                                 <  1:3662406/93‑1 (MQ=255)
                                                         tAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCgg                                <  1:2362050/93‑1 (MQ=255)
                                                         tAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCgg                                >  2:3696758/1‑93 (MQ=255)
                                                           gcgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGc                              <  2:1005129/93‑1 (MQ=255)
                                                            ctcccTTCGTCTTTTAGCGCCTGAATAGAAGGGCGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             <  2:8346771/91‑1 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             <  2:4292116/93‑1 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             <  2:8226701/93‑1 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             <  1:9926358/93‑1 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             >  1:339556/1‑93 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             >  1:5298033/1‑93 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             <  2:137175/93‑1 (MQ=255)
                                                            cgcCCTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCa                             <  1:9701652/93‑1 (MQ=255)
                                                              cccTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCAtt                           >  1:6999488/1‑93 (MQ=255)
                                                              cccTTCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCAtt                           >  1:7245624/1‑93 (MQ=255)
                                                               ccTTCATCTTTTAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTc                          >  1:3947847/1‑93 (MQ=255)
                                                                 ttCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATACTCAAACGCGCGGGCATTCTg                        >  1:8267831/1‑93 (MQ=255)
                                                                  tCATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGa                       <  2:5050802/93‑1 (MQ=255)
                                                                   cATCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGaa                      <  2:9676516/93‑1 (MQ=255)
                                                                    aTCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGaaa                     >  1:9588384/1‑93 (MQ=255)
                                                                     tcatttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAg                    <  1:7443406/90‑1 (MQ=255)
                                                                     tCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAg                    >  1:4526170/1‑93 (MQ=255)
                                                                     tCTTTTAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAg                    >  1:2230269/1‑93 (MQ=255)
                                                                       ttttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGc                  >  1:9159827/1‑93 (MQ=255)
                                                                       ttttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGc                  <  1:5595668/93‑1 (MQ=255)
                                                                       ttttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGc                  >  2:9434468/1‑93 (MQ=255)
                                                                       ttttAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGc                  >  2:5366499/1‑93 (MQ=255)
                                                                        tttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCg                 >  2:5202941/1‑93 (MQ=255)
                                                                        tttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCg                 >  1:4845174/1‑93 (MQ=255)
                                                                         ttAGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGa                <  2:2940100/93‑1 (MQ=255)
                                                                          tAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGaa               >  2:1900317/1‑93 (MQ=255)
                                                                           aGCGCCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAAt              >  2:1443741/1‑93 (MQ=255)
                                                                            gcgcCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCATATATTCAAACGCGCGGGCATTCTGAAAGGCGAAtt             <  1:1680694/93‑1 (MQ=255)
                                                                             cgcCTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAAttt            <  2:675185/93‑1 (MQ=255)
                                                                               ccTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTa          >  1:9028993/1‑93 (MQ=255)
                                                                                cTGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTaa         >  1:5450021/1‑93 (MQ=255)
                                                                                 tGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTCAg        >  2:759121/1‑93 (MQ=255)
                                                                                 tGAATAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTAAg        >  2:3353677/1‑93 (MQ=255)
                                                                                    aTAGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTAAgcgc     >  2:1609827/1‑93 (MQ=255)
                                                                                      aGAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTAAGCGCCg   <  1:5150665/93‑1 (MQ=255)
                                                                                       gAAGGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTAAGCGCCgg  >  2:6040168/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGCGCCCTTCATCTTTTAGCGCCTGAATAGAAAGGGGACCAAAAACTTCTTCCGGCGTGGAGAAGCGGGTCATCAGATATTCAAACGCGCGGGCATTCTGAAAGGCGAATTTTAAGCGCCGG  >  NC_000913/3928374‑3928553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑