BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	54,157	C→T	P182P (CCG→CCA)	surA ←	survival protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	54,157	0	C	T	99.3%	1099.4 / ‑6.0	305	P182P (CCG→CCA)	surA	survival protein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (169/134); total (170/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

GAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAG  >  NC_000913/54067‑54246

gAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTgg                                                                                         >  2:5582715/1‑93 (MQ=255)

gAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTgg                                                                                         >  1:7219794/1‑93 (MQ=255)

gAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGCTTTCCGGCAGTgg                                                                                         >  1:3618161/1‑93 (MQ=255)

 aaaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTggg                                                                                        <  2:505253/93‑1 (MQ=255)

 aaaTCAGCGCCGCTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTggg                                                                                        <  2:2900028/93‑1 (MQ=255)

  aaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGa                                                                                       >  1:5597360/1‑93 (MQ=255)

  aaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGa                                                                                       <  1:3372294/93‑1 (MQ=255)

  aaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGa                                                                                       >  2:3459046/1‑93 (MQ=255)

   aTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGAt                                                                                      <  2:6326578/93‑1 (MQ=255)

    tCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATc                                                                                     >  1:6851200/1‑93 (MQ=255)

     cAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCa                                                                                    >  1:1572648/1‑93 (MQ=255)

      aGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAg                                                                                   <  2:6504494/93‑1 (MQ=255)

       gcgcCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAgg                                                                                  <  1:2144748/93‑1 (MQ=255)

        cgcCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGa                                                                                 <  1:3007221/93‑1 (MQ=255)

            gTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGTAGTGGGATCAGGAtgtg                                                                             >  2:4642405/1‑93 (MQ=255)

             ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTgg                                                                            >  2:5856271/1‑93 (MQ=255)

             ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTgg                                                                            <  1:8730161/93‑1 (MQ=255)

             ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTgg                                                                            >  1:4227044/1‑93 (MQ=255)

             ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGCTTTCCGGCAGTGGGATCAGGATGTgg                                                                            >  1:8207050/1‑93 (MQ=255)

                  cgcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAg                                                                       <  1:6166749/93‑1 (MQ=255)

                   gcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAgg                                                                      <  2:4434118/93‑1 (MQ=255)

                   gcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAgg                                                                      <  2:2465986/93‑1 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  2:1077587/1‑93 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  1:4754954/1‑93 (MQ=255)

                     cTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGtt                                                                    <  1:6050414/93‑1 (MQ=255)

                      tGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTc                                                                   >  2:3230733/1‑93 (MQ=255)

                       gATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTcg                                                                  >  1:75597/1‑92 (MQ=255)

                        aTCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAg                                                                 <  1:5353879/93‑1 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                >  2:2590557/1‑93 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                <  2:7402581/93‑1 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                <  1:1214988/93‑1 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                >  2:8854087/1‑93 (MQ=255)

                          cGACAATGGCGCGCGCCTTGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGGTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCt                                                               >  2:8423529/1‑93 (MQ=255)

                          cGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGATTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGcc                                                               >  1:2462965/1‑92 (MQ=255)

                            aCAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCa                                                             <  1:1779515/93‑1 (MQ=255)

                            aCAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGATCAGCTCa                                                             >  2:687415/1‑93 (MQ=255)

                             cAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAg                                                            <  2:2159654/93‑1 (MQ=255)

                             cAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAg                                                            <  2:7196332/93‑1 (MQ=255)

                             cAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAg                                                            >  1:7053800/1‑93 (MQ=255)

                              aaTGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGt                                                           >  2:9094549/1‑93 (MQ=255)

                                tGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGc                                                         >  1:6389530/1‑93 (MQ=255)

                                tGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGc                                                         >  2:8168497/1‑93 (MQ=255)

                                 ggCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCt                                                        >  1:3114198/1‑93 (MQ=255)

                                  gcgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTg                                                       >  2:4186750/1‑93 (MQ=255)

                                  gcgcgcgcCTGGCGTGCCGCTGCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTg                                                       <  1:2631795/93‑1 (MQ=255)

                                   cgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTgg                                                      <  1:9073565/93‑1 (MQ=255)

                                     cgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcg                                                    >  2:2515935/1‑93 (MQ=255)

                                        gcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgtcg                                                 <  2:9159157/93‑1 (MQ=255)

                                         ccTGGCTTTCCGCTTCTCTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGTGATCTGGTTGTGGCTCAGGTTTAGCTCAGTGCAGTcgtcgt                                                >  2:8988722/1‑93 (MQ=255)

                                         ccTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgtcgt                                                >  2:5001001/1‑93 (MQ=255)

                                          cTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtt                                               >  1:1915239/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              <  1:1019825/93‑1 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  1:81055/1‑93 (MQ=255)

                                            ggCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt                                             <  1:2961646/93‑1 (MQ=255)

                                            ggCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt                                             >  1:6684987/1‑93 (MQ=255)

                                             gCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTg                                            >  2:6432464/1‑93 (MQ=255)

                                             gCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTg                                            <  1:198563/93‑1 (MQ=255)

                                              cTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTgg                                           >  2:8136851/1‑93 (MQ=255)

                                               tttCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGt                                          >  2:2760141/1‑93 (MQ=255)

                                                 tCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTa                                        <  1:8909992/93‑1 (MQ=255)

                                                  ccGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAc                                       <  1:8281291/93‑1 (MQ=255)

                                                   cGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAcc                                      <  2:7649297/93‑1 (MQ=255)

                                                   cGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAcc                                      <  1:8423813/93‑1 (MQ=255)

                                                   cGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAcc                                      >  1:8547931/1‑93 (MQ=255)

                                                   cGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTAGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAcc                                      <  1:4382713/93‑1 (MQ=255)

                                                     cTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa                                    >  2:5460437/1‑93 (MQ=255)

                                                      tttgTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAGGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc                                   <  2:8895682/90‑1 (MQ=255)

                                                      ttCGTTCACCTGATCAGAGGTCGGGTTTGCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc                                   <  1:7102152/93‑1 (MQ=255)

                                                       tCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAcc                                  <  2:9267537/93‑1 (MQ=255)

                                                         gTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctg                                >  2:3938015/1‑93 (MQ=255)

                                                         gTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctg                                >  1:3297331/1‑93 (MQ=255)

                                                          ttCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgc                               <  1:4824725/93‑1 (MQ=255)

                                                          ttCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgc                               >  1:6560548/1‑93 (MQ=255)

                                                          ttCACCGGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgc                               <  1:958925/93‑1 (MQ=255)

                                                           tCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgct                              <  1:2355397/93‑1 (MQ=255)

                                                           tCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTCGGTTACCCACctgct                              >  1:8951546/1‑93 (MQ=255)

                                                            cACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgctg                             >  2:1313057/1‑93 (MQ=255)

                                                                tGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGcc                         >  1:9419541/1‑93 (MQ=255)

                                                                tGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGcc                         >  1:879526/1‑93 (MQ=255)

                                                                 gATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGccg                        >  2:8363640/1‑92 (MQ=255)

                                                                 gATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCa                        >  1:4029043/1‑93 (MQ=255)

                                                                  aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       <  2:7117806/93‑1 (MQ=255)

                                                                   tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAgg                      >  1:5680380/1‑93 (MQ=255)

                                                                   tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAgg                      >  2:2837301/1‑93 (MQ=255)

                                                                    cAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAggg                     >  1:2525497/1‑93 (MQ=255)

                                                                    cAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAggg                     <  2:7943982/93‑1 (MQ=255)

                                                                    cAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAggg                     <  2:8572612/93‑1 (MQ=255)

                                                                    cAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAggg                     <  2:5355578/93‑1 (MQ=255)

                                                                     agagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGa                    <  2:409519/93‑1 (MQ=255)

                                                                     agagGTCGAGTTTTGCGGCAGTGGGAGCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGa                    <  1:6291673/93‑1 (MQ=255)

                                                                       agGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGAtt                  <  2:308342/93‑1 (MQ=255)

                                                                         gTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCg                >  1:1168247/1‑93 (MQ=255)

                                                                         gTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCg                <  1:3848792/93‑1 (MQ=255)

                                                                         gTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGACTCg                >  2:6199187/1‑93 (MQ=255)

                                                                            gggTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACt             >  2:4754327/1‑93 (MQ=255)

                                                                             ggTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACtt            >  1:5714319/1‑93 (MQ=255)

                                                                             ggTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACtt            >  1:7600509/1‑93 (MQ=255)

                                                                             ggTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACtt            >  1:928613/1‑93 (MQ=255)

                                                                                tttCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCt         <  1:2670121/93‑1 (MQ=255)

                                                                                      gCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCCCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCa   >  1:9722176/1‑93 (MQ=255)

GAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAG  >  NC_000913/54067‑54246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`