Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,962,662 A→G M195T (ATG→ACG)  rhlB ← putative ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,962,6620AG99.8% 1416.7 / ‑5.9 441M195T (ATG→ACG) rhlBputative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (149/291);  total (149/292)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 465 total aligned reads displayed.

TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751
                                                                                          |                                                                                         
tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTg                                                                                         <  1:8797602/93‑1 (MQ=255)
tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTg                                                                                         >  1:1516941/1‑93 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      >  2:3776387/1‑93 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      >  2:78440/1‑93 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      <  1:9059985/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  2:5141974/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     >  2:713339/1‑93 (MQ=255)
     cGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAg                                                                                    <  2:9312542/93‑1 (MQ=255)
      ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                   >  1:4620017/1‑93 (MQ=255)
      ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                   <  1:8445091/93‑1 (MQ=255)
      ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                   <  1:3657583/93‑1 (MQ=255)
       gTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgc                                                                                  <  2:4524492/93‑1 (MQ=255)
        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGGGTTGAGgcg                                                                                 >  1:3336694/1‑93 (MQ=255)
         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                <  1:2013475/93‑1 (MQ=255)
          ccACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCt                                                                               >  2:6812258/1‑93 (MQ=255)
            aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTgg                                                                             >  2:5031311/1‑93 (MQ=255)
            aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTgg                                                                             >  2:2307340/1‑93 (MQ=255)
             cTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGt                                                                            >  1:1762220/1‑93 (MQ=255)
               tCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGttt                                                                          <  2:4676319/93‑1 (MQ=255)
               tCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGttt                                                                          <  2:1125336/93‑1 (MQ=255)
                cAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTg                                                                         >  1:2738431/1‑93 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  2:6868599/93‑1 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  1:7991891/93‑1 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  1:9899324/93‑1 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       >  2:7515135/1‑93 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  2:8941760/93‑1 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGGACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  1:5454376/1‑93 (MQ=255)
                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     <  2:3474605/93‑1 (MQ=255)
                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     <  2:4152456/93‑1 (MQ=255)
                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   >  1:1006659/1‑93 (MQ=255)
                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   <  2:3262279/93‑1 (MQ=255)
                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   >  1:6505649/1‑93 (MQ=255)
                       ttCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcgg                                                                  >  1:739281/1‑93 (MQ=255)
                       ttCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcgg                                                                  <  1:8533354/93‑1 (MQ=255)
                         cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                                >  1:5176654/1‑93 (MQ=255)
                          gggaTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTTAGGCGCTGGTTTGCAGGCGGCAt                                                               <  1:301493/90‑1 (MQ=255)
                          ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAt                                                               <  1:1000280/93‑1 (MQ=255)
                          ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAt                                                               <  2:1936132/93‑1 (MQ=255)
                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              <  1:6117846/93‑1 (MQ=255)
                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              >  2:4655263/1‑93 (MQ=255)
                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              <  1:2190731/93‑1 (MQ=255)
                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              >  2:9399529/1‑93 (MQ=255)
                              ttGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcgg                                                           >  1:1144386/1‑93 (MQ=255)
                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  1:656578/1‑93 (MQ=255)
                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  1:4109780/1‑93 (MQ=255)
                                 ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcgg                                                        >  1:5916400/1‑93 (MQ=255)
                                 ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcgg                                                        >  1:3211584/1‑93 (MQ=255)
                                 ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcgg                                                        >  2:5822777/1‑93 (MQ=255)
                                   cATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                      <  1:183495/93‑1 (MQ=255)
                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                     <  1:7431828/93‑1 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    >  2:102156/1‑93 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    >  1:2385976/1‑93 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGAGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGAATACGGCGGAAca                                                    >  1:757881/1‑93 (MQ=255)
                                      cTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcag                                                   >  1:1325322/1‑93 (MQ=255)
                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  2:864798/93‑1 (MQ=255)
                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  1:9203353/93‑1 (MQ=255)
                                          tCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccag                                               <  2:265375/93‑1 (MQ=255)
                                           cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccagc                                              <  1:9541099/93‑1 (MQ=255)
                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                             >  1:7809077/1‑93 (MQ=255)
                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                             <  1:4667950/93‑1 (MQ=255)
                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                             >  1:7070911/1‑93 (MQ=255)
                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGatatt                                       >  1:248294/1‑92 (MQ=255)
                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCCGATATc                                       >  2:4060763/1‑93 (MQ=255)
                                                   aGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCt                                      <  2:9495251/93‑1 (MQ=255)
                                                    gTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCtt                                     <  1:3500275/93‑1 (MQ=255)
                                                    gTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCtt                                     <  2:6389605/93‑1 (MQ=255)
                                                     ttCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCttt                                    >  2:8841922/1‑93 (MQ=255)
                                                     ttCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCttt                                    <  2:9717721/93‑1 (MQ=255)
                                                      tCACGTGCCCGGTACGAAAGCGTGGCTGATAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTta                                   <  1:4915940/93‑1 (MQ=255)
                                                      tCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTta                                   <  2:2972118/93‑1 (MQ=255)
                                                          gTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaataa                               >  1:683148/1‑93 (MQ=255)
                                                          gTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaataa                               <  1:4310182/93‑1 (MQ=255)
                                                          gTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaataa                               <  2:628244/93‑1 (MQ=255)
                                                           tACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaa                              >  2:8560112/1‑93 (MQ=255)
                                                             cccGGTACGAAAGCGTGTCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGc                            <  1:1657287/93‑1 (MQ=255)
                                                             cccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGc                            <  1:4796276/93‑1 (MQ=255)
                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                           <  2:4403110/93‑1 (MQ=255)
                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                           <  2:2744265/93‑1 (MQ=255)
                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                           <  2:6563771/93‑1 (MQ=255)
                                                                ggTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                         >  2:1588669/1‑93 (MQ=255)
                                                                  tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                       <  2:134800/93‑1 (MQ=255)
                                                                  gaCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                       <  2:7387972/92‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:9355505/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  2:3080499/93‑1 (MQ=255)
                                                                    cGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                     <  2:8203492/93‑1 (MQ=255)
                                                                     gAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCg                    >  2:3170913/1‑93 (MQ=255)
                                                                       aaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTa                  <  1:6385222/93‑1 (MQ=255)
                                                                         gCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa                <  2:8431379/93‑1 (MQ=255)
                                                                         gCGGGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa                <  2:2414736/93‑1 (MQ=255)
                                                                          cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt               <  1:9652333/93‑1 (MQ=255)
                                                                          cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt               <  2:5341160/93‑1 (MQ=255)
                                                                            tGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc             >  2:6068117/1‑93 (MQ=255)
                                                                            tGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc             <  2:9431315/93‑1 (MQ=255)
                                                                              gCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGa           <  1:7166990/93‑1 (MQ=255)
                                                                               cGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGAt          <  1:5707991/93‑1 (MQ=255)
                                                                                    aaCAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt     >  1:5579629/1‑93 (MQ=255)
                                                                                     aCAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCtt    >  1:8304648/1‑93 (MQ=255)
                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg  <  2:8019285/93‑1 (MQ=255)
                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg  <  1:382228/93‑1 (MQ=255)
                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg  >  2:3650048/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑