Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,245 T→C Y72H (TAC→CAC)  rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,2450TC99.8% 1548.7 / NA 487Y72H (TAC→CAC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (243/243);  minor base A (0/1);  total (243/244)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 501 total aligned reads displayed.

CACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATT  >  NC_000913/3964155‑3964334
                                                                                          |                                                                                         
caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCAc                                                                                         >  2:961255/1‑93 (MQ=255)
caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCAc                                                                                         >  2:3992789/1‑93 (MQ=255)
caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCAc                                                                                         <  1:7573437/93‑1 (MQ=255)
 actcAAAGAGCGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTTCAGGATGGATTTGGTTTCCTCCGTTCCTCAGACAGCTTCCAcc                                                                                        >  2:8959508/4‑93 (MQ=255)
 aCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCAcc                                                                                        <  2:5680394/93‑1 (MQ=255)
  cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCt                                                                                       >  2:6812474/1‑93 (MQ=255)
  cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCt                                                                                       <  1:8844783/93‑1 (MQ=255)
     aaaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGc                                                                                    <  2:5035475/93‑1 (MQ=255)
       agagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCg                                                                                  >  1:8613164/1‑93 (MQ=255)
        gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCgg                                                                                 <  2:3606297/93‑1 (MQ=255)
          gTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTc                                                                               <  1:1137534/93‑1 (MQ=255)
           tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGtac                                                                              >  2:2626217/1‑91 (MQ=255)
           tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTcc                                                                              <  1:9055632/93‑1 (MQ=255)
           tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTcc                                                                              <  1:9805930/93‑1 (MQ=255)
           tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTcc                                                                              <  2:3377316/93‑1 (MQ=255)
           tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTcc                                                                              <  1:4482101/93‑1 (MQ=255)
            ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCt                                                                             >  1:6981712/1‑93 (MQ=255)
            ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCt                                                                             <  2:8887398/93‑1 (MQ=255)
             gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtg                                                                            >  2:2769845/1‑93 (MQ=255)
              cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtga                                                                           <  1:10017030/93‑1 (MQ=255)
              cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtga                                                                           <  1:4249596/93‑1 (MQ=255)
              cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtga                                                                           <  2:3717836/93‑1 (MQ=255)
               gAAGATATCTTTGGTGATGGCTTACTGGAGATATTTCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgat                                                                          >  1:1825612/1‑93 (MQ=255)
                aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatg                                                                         >  1:4879229/1‑93 (MQ=255)
                 aGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatga                                                                        <  2:9307351/93‑1 (MQ=255)
                 aGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatga                                                                        <  1:6868532/93‑1 (MQ=255)
                   atatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACa                                                                      >  2:2976084/1‑93 (MQ=255)
                   atatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACa                                                                      <  2:7935180/93‑1 (MQ=255)
                    tatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACAt                                                                     >  1:9384649/1‑93 (MQ=255)
                     atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATc                                                                    >  2:3510344/1‑93 (MQ=255)
                       cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTa                                                                  <  2:104999/93‑1 (MQ=255)
                       cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTa                                                                  >  1:2457163/1‑93 (MQ=255)
                         ttGGTGATGGCGTACTGGAGATGTTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACg                                                                >  2:6410976/1‑93 (MQ=255)
                          tGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGt                                                               >  2:8152872/1‑93 (MQ=255)
                          tGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGt                                                               <  2:7002645/93‑1 (MQ=255)
                          tGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGt                                                               >  1:7986517/1‑93 (MQ=255)
                            gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGttt                                                             >  2:1559041/1‑93 (MQ=255)
                             ggATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTc                                                            <  1:1440364/92‑1 (MQ=255)
                               gtGGCGTACTGGAGATATTGCAGGATGGAGTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTccc                                                          <  2:3885437/92‑1 (MQ=255)
                               aTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTccc                                                          >  2:4635418/1‑93 (MQ=255)
                                tGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTcccc                                                         <  1:8410144/93‑1 (MQ=255)
                                tGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTcccc                                                         <  1:3079249/93‑1 (MQ=255)
                                 ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCt                                                        >  1:7275410/1‑93 (MQ=255)
                                  gCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTa                                                       <  2:4415421/93‑1 (MQ=255)
                                    gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCCCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGc                                                     <  1:5005314/93‑1 (MQ=255)
                                    gTACTGGAGATATTGCAGGATGACTTTGGTTTGCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGc                                                     <  2:5504413/93‑1 (MQ=255)
                                     tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGcc                                                    >  2:3157596/1‑93 (MQ=255)
                                     tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGcc                                                    >  1:9475661/1‑93 (MQ=255)
                                      aCTGGAGATATTGGAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCa                                                   >  1:6718373/1‑93 (MQ=255)
                                       cTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaa                                                  >  1:9958437/1‑93 (MQ=255)
                                        tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                                                 >  1:8244552/1‑93 (MQ=255)
                                        tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                                                 >  2:2229933/1‑93 (MQ=255)
                                        tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                                                 >  1:5914897/1‑93 (MQ=255)
                                         ggAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAAt                                                >  1:2676757/1‑93 (MQ=255)
                                           agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcc                                              >  2:756931/1‑93 (MQ=255)
                                            gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccg                                             <  2:6047465/93‑1 (MQ=255)
                                             atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgc                                            <  1:2075012/93‑1 (MQ=255)
                                             atagTGCAGGATGGATGTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgc                                            <  2:8556562/93‑1 (MQ=255)
                                               atTGCAGGATGGATTTGGTTTCCTCCGTTTCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgcgg                                          >  2:830912/1‑91 (MQ=255)
                                               atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgccg                                          >  2:1716026/1‑93 (MQ=255)
                                                   cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTAAGTTTCCCCTAGCCAACTCCGCCGTTTc                                      >  2:5899708/1‑93 (MQ=255)
                                                     ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCaa                                    >  2:515541/1‑93 (MQ=255)
                                                      gatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAAc                                   <  2:180651/93‑1 (MQ=255)
                                                       atggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAAcc                                  >  1:7673513/1‑93 (MQ=255)
                                                        tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCt                                 >  1:5216834/1‑93 (MQ=255)
                                                         ggctttGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGCTTCCCCTAGCCAAATCCGCCGTTTCAACCTc                                >  2:5252882/4‑93 (MQ=255)
                                                           atTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCg                              <  1:4301319/93‑1 (MQ=255)
                                                             ttGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCa                            <  2:7214495/93‑1 (MQ=255)
                                                             ttGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCa                            <  2:1381190/93‑1 (MQ=255)
                                                             ttGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCa                            >  1:620475/1‑93 (MQ=255)
                                                               ggTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACt                          <  1:1923593/93‑1 (MQ=255)
                                                               ggTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACt                          <  1:5439003/93‑1 (MQ=255)
                                                               ggTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACt                          >  1:5603595/1‑93 (MQ=255)
                                                                gTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTg                         <  1:1485440/93‑1 (MQ=255)
                                                                 tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTgg                        <  1:2230239/93‑1 (MQ=255)
                                                                  ttCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGt                       <  2:4782826/93‑1 (MQ=255)
                                                                   tcctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTg                      <  2:7726378/93‑1 (MQ=255)
                                                                    cctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGa                     <  2:1944428/93‑1 (MQ=255)
                                                                    cctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGa                     <  1:3611725/93‑1 (MQ=255)
                                                                    cctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGa                     >  1:6615497/1‑93 (MQ=255)
                                                                    cctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTCCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGa                     <  2:5449151/93‑1 (MQ=255)
                                                                    cctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTCCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGa                     <  2:1231251/93‑1 (MQ=255)
                                                                     ctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGAt                    >  2:1448547/1‑93 (MQ=255)
                                                                         gTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCa                >  1:3884145/1‑93 (MQ=255)
                                                                         gTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCa                >  2:7816364/1‑93 (MQ=255)
                                                                         gTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCa                <  2:7436996/93‑1 (MQ=255)
                                                                            ccGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtct             <  1:2979711/93‑1 (MQ=255)
                                                                            ccGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtct             <  2:6574611/93‑1 (MQ=255)
                                                                             cGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc            <  1:3518571/93‑1 (MQ=255)
                                                                              gCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGCTACCATctct           >  1:4663029/1‑93 (MQ=255)
                                                                              gCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATctct           >  2:6195244/1‑93 (MQ=255)
                                                                              gCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATctct           <  2:9127540/93‑1 (MQ=255)
                                                                               cAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTg          <  2:4248783/93‑1 (MQ=255)
                                                                                 gACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGGCAAATCCGCCGTTTCAACCTCCGCACTTGTGATACCATCTCTGGt        >  2:6121026/1‑93 (MQ=255)
                                                                                 gACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGt        <  1:156241/93‑1 (MQ=255)
                                                                                   cAGCTCCCCCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTaa      <  2:8673124/93‑1 (MQ=255)
                                                                                    aGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg     <  1:3262281/93‑1 (MQ=255)
                                                                                       tCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt  >  1:2890887/1‑93 (MQ=255)
                                                                                       tCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt  >  1:8967312/1‑93 (MQ=255)
                                                                                       tCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt  >  1:4627252/1‑93 (MQ=255)
                                                                                          |                                                                                         
CACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATT  >  NC_000913/3964155‑3964334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑