BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,211	T→C	T473A (ACC→GCC)	acrB ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,211	0	T	C	99.7%	1087.3 / NA	331	T473A (ACC→GCC)	acrB	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (158/172); minor base A (0/1); total (158/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

CTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGA  >  NC_000913/482121‑482300

cTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCaa                                                                                         <  2:3579730/93‑1 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                        >  2:1513276/1‑93 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                        >  2:6890321/1‑93 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                        >  2:3265344/1‑93 (MQ=255)

  ttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATa                                                                                       >  2:1675862/1‑93 (MQ=255)

   tGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATag                                                                                      >  2:1796439/1‑93 (MQ=255)

   tGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATag                                                                                      <  2:4203259/93‑1 (MQ=255)

     gCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATagag                                                                                    <  1:6881980/93‑1 (MQ=255)

     gCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATagag                                                                                    >  1:9737251/1‑93 (MQ=255)

      cAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAgaga                                                                                   <  1:2139079/93‑1 (MQ=255)

        aTCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAAc                                                                                 <  1:5504965/93‑1 (MQ=255)

        aTCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAAc                                                                                 >  1:7308330/1‑93 (MQ=255)

         tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACt                                                                                <  2:3941107/93‑1 (MQ=255)

         tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACt                                                                                <  1:1643406/93‑1 (MQ=255)

            gTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGAc                                                                             <  1:4021340/93‑1 (MQ=255)

             tttCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACg                                                                            >  1:4183973/1‑93 (MQ=255)

               tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgat                                                                          <  2:4951521/93‑1 (MQ=255)

               tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgat                                                                          <  2:1276665/93‑1 (MQ=255)

                  gCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgataga                                                                       <  2:6614885/93‑1 (MQ=255)

                   cATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagat                                                                      >  1:3878221/1‑93 (MQ=255)

                   cATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagat                                                                      >  1:6212602/1‑93 (MQ=255)

                   cATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagat                                                                      <  2:6989087/93‑1 (MQ=255)

                    aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagata                                                                     <  2:7865695/93‑1 (MQ=255)

                    aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagata                                                                     >  1:9574778/1‑93 (MQ=255)

                    aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagata                                                                     >  2:5305807/1‑93 (MQ=255)

                    aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagata                                                                     <  2:7102733/93‑1 (MQ=255)

                    aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagata                                                                     <  2:5549525/93‑1 (MQ=255)

                     tggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGatagatag                                                                    >  1:1972628/1‑93 (MQ=255)

                     tggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGatagatag                                                                    <  1:7155565/93‑1 (MQ=255)

                     tggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGatagatag                                                                    >  1:8824944/1‑93 (MQ=255)

                     tggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGatagatag                                                                    >  2:5804161/1‑93 (MQ=255)

                      ggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGc                                                                   >  2:2282478/1‑93 (MQ=255)

                        tggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCAc                                                                 <  1:6944424/93‑1 (MQ=255)

                         ggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCAcc                                                                <  1:6148692/93‑1 (MQ=255)

                          gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCa                                                               <  2:2722907/93‑1 (MQ=255)

                          gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCa                                                               <  2:6205948/93‑1 (MQ=255)

                             caAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTa                                                            <  1:4447453/93‑1 (MQ=255)

                             caAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTa                                                            <  1:6558804/93‑1 (MQ=255)

                             aaaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTa                                                            <  1:6353177/92‑1 (MQ=255)

                                agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGaa                                                         >  1:9850807/1‑93 (MQ=255)

                                agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGaa                                                         >  2:3020901/1‑93 (MQ=255)

                                      ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                   <  1:1367266/93‑1 (MQ=255)

                                       gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaa                                                  <  1:5161261/93‑1 (MQ=255)

                                        aGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa                                                 >  1:2500498/1‑93 (MQ=255)

                                          tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                               <  2:9604385/93‑1 (MQ=255)

                                          tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                               >  2:3215075/1‑93 (MQ=255)

                                           cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                              <  2:8003830/93‑1 (MQ=255)

                                           cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                              <  1:2287858/93‑1 (MQ=255)

                                           cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                              <  2:903918/93‑1 (MQ=255)

                                            aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaag                                             >  1:1855972/1‑93 (MQ=255)

                                             ggATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAgg                                            <  1:8493248/93‑1 (MQ=255)

                                              gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc                                           >  1:6483655/1‑93 (MQ=255)

                                               aTCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGcc                                          <  2:8342508/93‑1 (MQ=255)

                                               aTCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACACGTAGAACCGCCAAAGAAGccc                                          >  1:3430328/1‑93 (MQ=255)

                                                tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCa                                         <  2:1365088/93‑1 (MQ=255)

                                                tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGACa                                         >  2:1012229/1‑93 (MQ=255)

                                                 cAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt                                        <  2:7483209/93‑1 (MQ=255)

                                                 cAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt                                        >  1:1183981/1‑93 (MQ=255)

                                                  aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATc                                       >  1:3680797/1‑93 (MQ=255)

                                                   aCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg                                      >  2:5652278/1‑93 (MQ=255)

                                                    cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg                                     <  2:211710/93‑1 (MQ=255)

                                                    cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg                                     <  2:8439120/93‑1 (MQ=255)

                                                      ccaccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                   <  2:10032094/93‑1 (MQ=255)

                                                       caccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc                                  <  1:6489446/93‑1 (MQ=255)

                                                         ccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGa                                <  1:7507616/93‑1 (MQ=255)

                                                          caGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa                               <  2:9441325/93‑1 (MQ=255)

                                                           aGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt                              <  2:5952468/93‑1 (MQ=255)

                                                           aGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt                              <  2:7736955/93‑1 (MQ=255)

                                                            gTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATa                             >  1:4950812/1‑93 (MQ=255)

                                                             tACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAc                            <  2:9770463/93‑1 (MQ=255)

                                                             tACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAc                            >  2:7590303/1‑93 (MQ=255)

                                                             tACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAc                            <  2:7333041/93‑1 (MQ=255)

                                                               ccGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccg                          <  1:1691246/93‑1 (MQ=255)

                                                                cGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc                         <  1:4811623/93‑1 (MQ=255)

                                                                 gACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcagcc                        >  1:1463218/1‑93 (MQ=255)

                                                                   cAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                      >  2:5506208/1‑93 (MQ=255)

                                                                   cAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                      >  1:5668141/1‑93 (MQ=255)

                                                                    aGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGAc                     >  1:102395/1‑93 (MQ=255)

                                                                    aGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGAc                     <  2:4817025/93‑1 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                    <  1:3272223/93‑1 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                    >  1:9294558/1‑93 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                    >  2:5120980/1‑93 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                    >  2:4659367/1‑93 (MQ=255)

                                                                       gcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGt                  >  1:5322571/1‑93 (MQ=255)

                                                                        ccATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTa                 >  1:7604279/1‑93 (MQ=255)

                                                                         cATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc                >  2:89537/1‑93 (MQ=255)

                                                                         cATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc                >  1:9800952/1‑93 (MQ=255)

                                                                          aTTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc               >  2:8572676/1‑93 (MQ=255)

                                                                          aTTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc               >  1:8043048/1‑93 (MQ=255)

                                                                           ttGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa              >  2:6822920/1‑93 (MQ=255)

                                                                           ttGCTGAAACAATAGATTTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGACATCGGTACGAATACCGCCGACAGTACCa              <  1:8981629/93‑1 (MQ=255)

                                                                               tGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc          <  1:3939757/93‑1 (MQ=255)

                                                                               tGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc          >  2:4725586/1‑93 (MQ=255)

                                                                                 aaaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa        >  1:5887107/1‑93 (MQ=255)

                                                                                  aaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt       <  1:307871/93‑1 (MQ=255)

                                                                                  aaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt       <  2:5198119/93‑1 (MQ=255)

                                                                                   aCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa      >  1:9492787/1‑93 (MQ=255)

                                                                                   aCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa      >  2:1430624/1‑93 (MQ=255)

                                                                                    cAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAc     <  1:7808490/93‑1 (MQ=255)

                                                                                     aaTGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc    >  1:8132866/1‑93 (MQ=255)

CTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGA  >  NC_000913/482121‑482300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`