Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 488,917 A→G E1053G (GAG→GGG)  aefA → putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913488,9170AG99.7% 1078.9 / ‑4.9 335E1053G (GAG→GGG) aefAputative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (176/157);  total (178/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

Only 100 of 342 total aligned reads displayed.

TGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACG  >  NC_000913/488827‑489006
                                                                                          |                                                                                         
tGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGc                                                                                         >  1:7003642/1‑93 (MQ=255)
tGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGc                                                                                         <  2:8786395/93‑1 (MQ=255)
tGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGc                                                                                         <  2:3756953/93‑1 (MQ=255)
 gAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCt                                                                                        >  1:3054444/1‑93 (MQ=255)
     gcggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGt                                                                                    >  2:8646404/1‑93 (MQ=255)
     gcggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGt                                                                                    >  1:9013417/1‑93 (MQ=255)
      cggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTc                                                                                   >  1:8826815/1‑93 (MQ=255)
            cTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTAtg                                                                             >  2:8323532/1‑93 (MQ=255)
            cTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTAtg                                                                             >  2:7023754/1‑93 (MQ=255)
            cTGAGCACCCAAGGGTGATACACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCGCGTTGGATCATGGGCTGCGTCTGTAtg                                                                             <  2:7622862/93‑1 (MQ=255)
              taGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTAtgtg                                                                           <  2:7159366/92‑1 (MQ=255)
              gAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTAtgtg                                                                           >  2:9019472/1‑93 (MQ=255)
              gAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTAtgtg                                                                           >  2:2230452/1‑93 (MQ=255)
               aGCACCCAAGGGTGATGCACGAACCACTGCCGGAAGTCTTCGTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTAtggtg                                                                          >  2:4229136/1‑90 (MQ=255)
               aGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGc                                                                          <  2:6452905/93‑1 (MQ=255)
               aGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGc                                                                          <  2:3078916/93‑1 (MQ=255)
                 cACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGt                                                                        <  2:6015208/93‑1 (MQ=255)
                 cACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGt                                                                        <  2:7265685/93‑1 (MQ=255)
                   cccAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCGCGTTGGATCATGGGCTGCGTCTGTATGTGCGTGa                                                                      >  1:1988798/1‑93 (MQ=255)
                   cccAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGa                                                                      >  2:2733833/1‑93 (MQ=255)
                    ccAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGaa                                                                     <  2:1498284/93‑1 (MQ=255)
                    ccAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGaa                                                                     <  1:9856228/93‑1 (MQ=255)
                     cAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAAc                                                                    >  1:3708053/1‑93 (MQ=255)
                     cAAGGGCGATGCACGAACCAATGCCGGAAGCCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAAc                                                                    <  2:3202484/93‑1 (MQ=255)
                      aaGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACt                                                                   >  2:4430761/1‑93 (MQ=255)
                      aaGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACt                                                                   <  2:1965852/93‑1 (MQ=255)
                       aGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTg                                                                  <  2:1821320/93‑1 (MQ=255)
                       aGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTg                                                                  >  1:9553415/1‑93 (MQ=255)
                       aGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTg                                                                  <  1:9216801/93‑1 (MQ=255)
                        gggTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGc                                                                 <  2:5241898/93‑1 (MQ=255)
                         ggTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTAGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCg                                                                <  2:9896494/93‑1 (MQ=255)
                          gTGACGCACGAAACAATGCCGGAAGTCTTCTTTACGGCATTTTGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGt                                                               <  2:6179638/93‑1 (MQ=255)
                           tGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTg                                                              >  2:6722629/1‑93 (MQ=255)
                           tGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGATGCGTCTGTATGTGCGGGAACTGCGTg                                                              >  1:4405954/1‑93 (MQ=255)
                             aTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGAc                                                            <  2:7923436/93‑1 (MQ=255)
                              tGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGAcc                                                           >  1:4039202/1‑93 (MQ=255)
                               gCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg                                                          >  2:9717283/1‑93 (MQ=255)
                                cACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGt                                                         <  2:9854704/93‑1 (MQ=255)
                                 aCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTa                                                        <  1:1654969/93‑1 (MQ=255)
                                   gAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCGCGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGt                                                      >  1:2371094/1‑93 (MQ=255)
                                   gAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGt                                                      <  1:4194190/93‑1 (MQ=255)
                                    aaacAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTc                                                     <  1:7583106/90‑1 (MQ=255)
                                    aaCCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTc                                                     >  1:5896292/1‑93 (MQ=255)
                                     aCCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCg                                                    >  2:9348845/1‑93 (MQ=255)
                                     aCCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCg                                                    >  2:1649814/1‑93 (MQ=255)
                                      ccAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGt                                                   >  1:843871/1‑93 (MQ=255)
                                      ccAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGt                                                   >  1:136223/1‑93 (MQ=255)
                                      ccAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGCAGTCGt                                                   <  1:6981076/93‑1 (MQ=255)
                                       cAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTa                                                  <  1:9268279/93‑1 (MQ=255)
                                        aaTGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                 <  2:7785614/93‑1 (MQ=255)
                                        aaTGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                 <  1:6030557/93‑1 (MQ=255)
                                          tGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTg                                               >  1:6847802/1‑93 (MQ=255)
                                            ccGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTc                                             <  1:2294995/93‑1 (MQ=255)
                                              ggAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGa                                           >  1:6581258/1‑93 (MQ=255)
                                              ggAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGa                                           >  1:7941599/1‑93 (MQ=255)
                                              ggAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGa                                           <  2:4519577/93‑1 (MQ=255)
                                              ggAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGa                                           <  2:8111235/93‑1 (MQ=255)
                                               gAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGAt                                          >  1:8717806/1‑93 (MQ=255)
                                               gAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGAt                                          >  1:6195156/1‑93 (MQ=255)
                                                aaGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATg                                         <  2:2074091/93‑1 (MQ=255)
                                                aaGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATg                                         >  2:8489594/1‑93 (MQ=255)
                                                 aGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGa                                        >  1:1020146/1‑93 (MQ=255)
                                                 aGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGa                                        <  1:8298098/93‑1 (MQ=255)
                                                    cttcttTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCt                                     >  2:4147489/1‑93 (MQ=255)
                                                     ttcttTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg                                    <  2:9565337/93‑1 (MQ=255)
                                                        tttACTGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAAc                                 <  1:5365911/93‑1 (MQ=255)
                                                          tACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCg                               >  2:5587205/1‑93 (MQ=255)
                                                            cGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTa                             <  1:1742105/93‑1 (MQ=255)
                                                             ggCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTAc                            >  2:5798816/1‑93 (MQ=255)
                                                              gCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt                           <  1:8935543/93‑1 (MQ=255)
                                                              gCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt                           <  2:5690601/93‑1 (MQ=255)
                                                              gCATTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt                           <  2:5366370/93‑1 (MQ=255)
                                                                aTTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTAt                         <  1:8151247/93‑1 (MQ=255)
                                                                aTTTGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTAt                         >  1:6352174/1‑93 (MQ=255)
                                                                 tttGGTGCCAGCGCGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATc                        >  2:2965234/1‑93 (MQ=255)
                                                                 tttGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATc                        >  1:2110986/1‑93 (MQ=255)
                                                                  ttGGTGCTAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCCTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                       <  2:3428826/93‑1 (MQ=255)
                                                                  ttGGTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                       >  1:5773176/1‑93 (MQ=255)
                                                                    ggTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGAt                     >  2:4912810/1‑93 (MQ=255)
                                                                    ggTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGAt                     <  1:8528173/93‑1 (MQ=255)
                                                                     gTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATc                    <  1:2811689/93‑1 (MQ=255)
                                                                     gTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATc                    <  2:6376042/93‑1 (MQ=255)
                                                                     gTGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATc                    >  1:7544674/1‑93 (MQ=255)
                                                                      tGCCAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCa                   <  2:6603470/93‑1 (MQ=255)
                                                                        ccAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCCGc                 >  1:5214550/1‑93 (MQ=255)
                                                                        ccAGCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGc                 >  1:1304937/1‑93 (MQ=255)
                                                                           gCACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCtgt              >  2:5145351/1‑93 (MQ=255)
                                                                            gaCGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCtgtg             <  1:4482977/92‑1 (MQ=255)
                                                                            cACGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCtgtg             >  2:4803382/1‑93 (MQ=255)
                                                                             aCGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGc            <  1:9235366/93‑1 (MQ=255)
                                                                             aCGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTACGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGc            <  1:4948141/93‑1 (MQ=255)
                                                                              cGTTGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTCCTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGcc           <  2:1155384/93‑1 (MQ=255)
                                                                                ttGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGt         <  2:2494274/93‑1 (MQ=255)
                                                                                ttGGATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGt         <  1:7811688/93‑1 (MQ=255)
                                                                                   gATCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaa      <  1:4557065/93‑1 (MQ=255)
                                                                                    aTCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaa     >  2:3644018/1‑93 (MQ=255)
                                                                                     tCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaaa    <  2:2323448/93‑1 (MQ=255)
                                                                                     tCATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaaa    >  1:3921586/1‑93 (MQ=255)
                                                                                      cATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAAc   >  2:1208181/1‑93 (MQ=255)
                                                                                      cATGGGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACAGTACTATCGATCAGCTGTGCCGTGAAAAc   <  1:3186817/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACG  >  NC_000913/488827‑489006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑