BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,182	T→C	I280T (ATC→ACC)	htpG →	chaperone Hsp90

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,182	0	T	C	99.7%	1058.3 / ‑5.1	327	I280T (ATC→ACC)	htpG	chaperone Hsp90
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (151/174); total (152/175)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01

Only 100 of 344 total aligned reads displayed.

ACAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGACGACG  >  NC_000913/495092‑495271

aCAAACACATCGCCCACGACGTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAccc                                                                                         <  2:7849098/93‑1 (MQ=255)

  aaaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCg                                                                                       <  2:6064860/93‑1 (MQ=255)

  aaaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCg                                                                                       <  2:5073416/93‑1 (MQ=255)

     cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTcc                                                                                    <  2:3811800/93‑1 (MQ=255)

     cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTcc                                                                                    >  2:395931/1‑93 (MQ=255)

     cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTcc                                                                                    >  2:5726284/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCTGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   <  2:1700602/93‑1 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   <  1:7340789/93‑1 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   <  2:5326504/93‑1 (MQ=255)

       caTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCa                                                                                  <  1:2646780/93‑1 (MQ=255)

       caTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTCCTGTACACCCCGTCCCa                                                                                  <  1:9625967/93‑1 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 >  1:9717982/1‑93 (MQ=255)

          cGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGc                                                                               <  2:8823353/93‑1 (MQ=255)

          cGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGc                                                                               >  1:713464/1‑93 (MQ=255)

            cccACGACTTTAATGACCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTc                                                                             <  2:2534314/93‑1 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            <  1:9347521/93‑1 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            <  2:6909309/93‑1 (MQ=255)

              cACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCg                                                                           >  2:4531339/1‑93 (MQ=255)

               aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGt                                                                          >  2:1246914/1‑93 (MQ=255)

                cGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTg                                                                         >  1:2094626/1‑93 (MQ=255)

                cGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCACGCTCCGTg                                                                         >  1:9034621/1‑93 (MQ=255)

                 gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTgg                                                                        >  1:5053777/1‑93 (MQ=255)

                 gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTgg                                                                        <  2:1087801/93‑1 (MQ=255)

                 gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTgg                                                                        <  2:9385836/93‑1 (MQ=255)

                  aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTggg                                                                       >  2:3446999/1‑93 (MQ=255)

                    tttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGat                                                                     >  2:9758817/1‑93 (MQ=255)

                     ttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGata                                                                    >  1:1805670/1‑93 (MQ=255)

                       aaTGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATAtg                                                                  >  1:1636922/1‑93 (MQ=255)

                         tGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATAtgtg                                                                <  1:3782694/93‑1 (MQ=255)

                         tGAGCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTTCTGTACACCCCGTCCCAGGCTCCGTGGGATAtgtg                                                                <  1:1492099/93‑1 (MQ=255)

                           aTCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGa                                                              >  1:9099347/1‑93 (MQ=255)

                           aTCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGa                                                              >  2:8606508/1‑93 (MQ=255)

                            tCCGCTGCCCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTCCACCAGCCTTCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGaa                                                             <  2:6185605/93‑1 (MQ=255)

                             ccGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAAc                                                            <  1:8819143/93‑1 (MQ=255)

                             ccGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAAc                                                            <  2:4994935/93‑1 (MQ=255)

                             ccGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAAc                                                            >  1:783098/1‑93 (MQ=255)

                               gCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcg                                                          >  1:8068232/1‑93 (MQ=255)

                                cTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgc                                                         <  1:3892643/93‑1 (MQ=255)

                                 tGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgcg                                                        <  2:2325094/93‑1 (MQ=255)

                                  gACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGa                                                       >  1:1342188/1‑93 (MQ=255)

                                   aCCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGac                                                      >  1:1920308/1‑92 (MQ=255)

                                   aCCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGAt                                                      >  2:8395601/1‑93 (MQ=255)

                                    ccTGGAGCCACAACCGTGCTGAAGGTAAGCAGGAGTACACCAGCCTACTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATc                                                     <  1:5677497/93‑1 (MQ=255)

                                      tGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCAt                                                   >  1:10012974/1‑93 (MQ=255)

                                       ggAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa                                                  <  2:7967050/93‑1 (MQ=255)

                                       ggAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa                                                  <  2:6611176/93‑1 (MQ=255)

                                        gAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATaa                                                 >  2:1458485/1‑93 (MQ=255)

                                           ccACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAaca                                              <  1:6933787/93‑1 (MQ=255)

                                           ccACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAaca                                              >  1:8694555/1‑93 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             >  1:9832938/1‑93 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             <  1:663237/93‑1 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             <  2:1808416/93‑1 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            >  1:8131075/1‑93 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            <  1:4871338/93‑1 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            >  1:4118135/1‑93 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            >  2:7609806/1‑93 (MQ=255)

                                                 ccGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCt                                        <  1:3121986/93‑1 (MQ=255)

                                                 ccGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGCCCCAGGCTCCGTGGGACATGTGGAACCGCGATCATAAACACGGCCt                                        >  1:7653662/1‑93 (MQ=255)

                                                   gtgtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGa                                      >  2:5417309/1‑93 (MQ=255)

                                                    tgtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGAGCATAAACACGGCCTGaa                                     >  2:3548949/1‑93 (MQ=255)

                                                     gtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGaaa                                    <  2:2168352/93‑1 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   >  2:763952/1‑93 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   >  1:6834454/1‑93 (MQ=255)

                                                       tGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACt                                  <  1:2762890/93‑1 (MQ=255)

                                                        gAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATACACACGGCCTGAAACTg                                 >  1:5968030/1‑93 (MQ=255)

                                                        gAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTg                                 >  1:9919215/1‑93 (MQ=255)

                                                         aaGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGt                                <  2:2856603/93‑1 (MQ=255)

                                                          aGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTa                               >  1:7451846/1‑93 (MQ=255)

                                                           ggTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTAt                              <  2:4337316/93‑1 (MQ=255)

                                                           agTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTAt                              <  1:3253674/92‑1 (MQ=255)

                                                            gTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATg                             <  2:3291364/93‑1 (MQ=255)

                                                              aaGCAGGAGTACACCAGCCTTCTGCACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGtt                           <  1:2329861/93‑1 (MQ=255)

                                                                  aGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGc                       <  1:6048405/93‑1 (MQ=255)

                                                                  aGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGc                       >  1:8396162/1‑93 (MQ=255)

                                                                  aGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGc                       >  2:7364572/1‑93 (MQ=255)

                                                                   ggAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCg                      >  1:6453797/1‑93 (MQ=255)

                                                                   ggAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCg                      >  1:8541031/1‑93 (MQ=255)

                                                                   ggAGTACACCAGCCTGCTGTACACCCCGTCCCAGGATCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCg                      >  2:9929134/1‑93 (MQ=255)

                                                                    gAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgt                     >  1:8194258/1‑93 (MQ=255)

                                                                      gTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgt                   <  2:4790068/93‑1 (MQ=255)

                                                                       tACACCAGCCTGCGGTGCACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtg                  <  1:4785642/93‑1 (MQ=255)

                                                                       tACACCAGCCTACTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtg                  <  2:9112401/93‑1 (MQ=255)

                                                                        acacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtgt                 <  1:8563460/93‑1 (MQ=255)

                                                                        acacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtgt                 >  1:5254443/1‑93 (MQ=255)

                                                                          acCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtc               <  2:8170127/93‑1 (MQ=255)

                                                                          acCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtc               <  1:9301610/93‑1 (MQ=255)

                                                                           ccAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGACTGAAACTGTATGTTCAGCGTGTGTtca              >  1:1141965/1‑93 (MQ=255)

                                                                           ccAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtca              <  2:3096296/93‑1 (MQ=255)

                                                                           ccAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtca              >  2:8919672/1‑93 (MQ=255)

                                                                             aGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatc            >  1:7066679/1‑93 (MQ=255)

                                                                              gCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatca           <  2:6087957/93‑1 (MQ=255)

                                                                              gCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatca           <  1:9533468/93‑1 (MQ=255)

                                                                              gCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatca           >  2:9127122/1‑93 (MQ=255)

                                                                                ctgctgTACACCCCGTCCCAGGCTCCGTGGGCTATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATg         >  2:10009061/1‑93 (MQ=255)

                                                                                ctgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATg         <  1:2055093/93‑1 (MQ=255)

                                                                                 tgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATgg        >  1:5617383/1‑93 (MQ=255)

                                                                                 tgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATgg        <  2:946025/93‑1 (MQ=255)

                                                                                 tgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATgg        <  1:417431/93‑1 (MQ=255)

                                                                                    tgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGgacg     <  1:4501266/93‑1 (MQ=255)

                                                                                       acacCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGacgacg  >  1:6133466/1‑93 (MQ=255)

ACAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGACGACG  >  NC_000913/495092‑495271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`