BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	500,613	A→G	E422G (GAG→GGG)	gsk →	inosine‑guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	500,613	0	A	G	99.7%	1077.1 / ‑5.1	339	E422G (GAG→GGG)	gsk	inosine‑guanosine kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (148/189); total (149/190)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 350 total aligned reads displayed.

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

cATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGc                                                                                         >  2:3933715/1‑93 (MQ=255)

   cGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTg                                                                                      >  1:5863308/1‑93 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                    <  2:9347504/93‑1 (MQ=255)

      tAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAg                                                                                   <  2:6227750/93‑1 (MQ=255)

       aGCGCATGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGa                                                                                  <  1:8897013/93‑1 (MQ=255)

         cgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACa                                                                                <  1:7011024/93‑1 (MQ=255)

         cgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACa                                                                                <  1:3744183/93‑1 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              >  1:7907418/1‑93 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              >  1:4847775/1‑93 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             <  2:4649108/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             >  1:9759921/1‑93 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGCTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             >  1:1891610/1‑93 (MQ=255)

             ggTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCt                                                                            >  2:2098242/1‑93 (MQ=255)

             ggTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCt                                                                            >  1:273153/1‑93 (MQ=255)

             ggTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCt                                                                            <  1:8075196/93‑1 (MQ=255)

              gtgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTg                                                                           >  2:3283024/1‑93 (MQ=255)

              gtgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTg                                                                           <  1:8660982/93‑1 (MQ=255)

               tgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTgg                                                                          >  1:2021629/1‑93 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGaa                                                                        <  1:4752896/93‑1 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCCGGGCGTGAAGACAGCCTGGaa                                                                        >  2:346683/1‑93 (MQ=255)

                  gtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAag                                                                       >  1:3996481/1‑93 (MQ=255)

                  gtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAag                                                                       <  2:4542510/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAaga                                                                      >  2:1558930/1‑93 (MQ=255)

                     agtatGCTAACCGTGTGAGCTATCGGGTGCTGAACCAGCATTCGCTTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGt                                                                    <  1:9570041/91‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTc                                                                   >  1:9181428/1‑93 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTc                                                                   <  1:7329875/93‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTc                                                                   <  1:115736/93‑1 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  >  1:6037258/1‑93 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTAc                                                               <  2:4372758/93‑1 (MQ=255)

                           cTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACt                                                              <  1:6409730/93‑1 (MQ=255)

                           cTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACt                                                              <  2:1189682/93‑1 (MQ=255)

                             aaCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTgg                                                            >  1:8825060/1‑93 (MQ=255)

                              aCCGTGTGAGCTATCAGGTACTGAACCAGCATTCTCCTCGTTTAACGCGCGTCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTggg                                                           <  2:4990856/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGa                                                          <  2:9715050/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGa                                                          <  2:7331182/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGa                                                          <  1:2669180/93‑1 (MQ=255)

                                 gtgtGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATc                                                        >  1:5172694/1‑93 (MQ=255)

                                  tgtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCg                                                       >  2:7571030/1‑93 (MQ=255)

                                  tgtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCg                                                       >  2:8102420/1‑93 (MQ=255)

                                  ggtgtGCTATCAGGTTCTTAATCAGCATTCACCTCGTTTAACGCGCGGATTTCCGGGGCGTGAAGACAGCCAGGAAGAGTCTTACTGGGATCg                                                       <  2:8704888/92‑1 (MQ=255)

                                   gtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGt                                                      >  1:4514320/1‑93 (MQ=255)

                                    tgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGtt                                                     >  1:3631604/1‑93 (MQ=255)

                                    tgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGtt                                                     >  1:9343621/1‑93 (MQ=255)

                                      atcTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTaa                                                   <  2:243351/91‑1 (MQ=255)

                                      aGCTATCAGTTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTaa                                                   <  2:4727525/93‑1 (MQ=255)

                                      aGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGGTTTACTGGGATCGTTaa                                                   >  2:8941637/1‑93 (MQ=255)

                                         tATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGtt                                                <  1:9119808/93‑1 (MQ=255)

                                          aTCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTa                                               <  2:6921068/93‑1 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              <  2:5953839/93‑1 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             <  1:7764451/93‑1 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             >  2:6687803/1‑93 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             <  1:9890036/93‑1 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             >  2:10007233/1‑93 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             <  2:2259176/93‑1 (MQ=255)

                                              ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           <  1:2167382/93‑1 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        >  1:4293953/1‑93 (MQ=255)

                                                   tGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGGCAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGtt                                      >  2:254286/1‑93 (MQ=255)

                                                   tGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGtt                                      >  2:5063765/1‑93 (MQ=255)

                                                   tGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGtt                                      <  1:3106064/93‑1 (MQ=255)

                                                    gAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTc                                     <  2:4472967/93‑1 (MQ=255)

                                                    gAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTc                                     <  1:8472140/93‑1 (MQ=255)

                                                    gAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTc                                     <  1:3157971/93‑1 (MQ=255)

                                                       ccAGCATTCGCCGCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  <  1:8596868/93‑1 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTGCTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  <  2:7506354/93‑1 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGtt                                  >  2:9007671/1‑92 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  <  2:2599433/93‑1 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 <  1:41752/93‑1 (MQ=255)

                                                          gCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGc                               >  2:2896423/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:1434448/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:741011/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  2:1307612/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:8191035/1‑93 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGGGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           >  2:3059064/1‑93 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           <  1:6505525/93‑1 (MQ=255)

                                                                cccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATa                         <  2:3787677/92‑1 (MQ=255)

                                                                aCCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATa                         <  2:6110106/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGTGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:7920638/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:956295/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:4226259/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        >  1:9092992/1‑93 (MQ=255)

                                                                  cTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAg                       >  2:5416359/1‑93 (MQ=255)

                                                                   tCGTTAAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAgg                      <  2:178711/93‑1 (MQ=255)

                                                                    cGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGc                     <  1:2816417/93‑1 (MQ=255)

                                                                     gTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCg                    <  2:1686114/93‑1 (MQ=255)

                                                                      tttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGt                   >  1:6901313/1‑93 (MQ=255)

                                                                      tttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGt                   >  1:5469009/1‑93 (MQ=255)

                                                                       ttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGtt                  <  1:5554469/93‑1 (MQ=255)

                                                                       ttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGtt                  <  2:9088283/93‑1 (MQ=255)

                                                                         aaCGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCa                <  1:2521483/93‑1 (MQ=255)

                                                                            gcgcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgc             <  2:9193591/93‑1 (MQ=255)

                                                                             cgcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgcc            <  1:1043909/93‑1 (MQ=255)

                                                                              gcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgccg           >  1:5806604/1‑93 (MQ=255)

                                                                               cgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgccgc          <  2:7730635/93‑1 (MQ=255)

                                                                                ggCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCa         >  1:90356/1‑93 (MQ=255)

                                                                                 gCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCAt        >  1:2213199/1‑93 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      <  1:6084535/93‑1 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      >  2:5244229/1‑93 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      >  1:1538797/1‑93 (MQ=255)

                                                                                     gCCGGGGCGTGGAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGATCGTAGGCCAGATAAGGCGTTCACGCAGCATCttg    >  2:5676648/1‑91 (MQ=38)

                                                                                     gCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTgg    <  1:4943296/93‑1 (MQ=255)

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`