BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,019,057	A→G	V415V (GTA→GTG)	yigR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,019,057	0	A	G	99.6%	1546.8 / ‑4.0	489	V415V (GTA→GTG)	yigR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (248/239); total (249/240)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 512 total aligned reads displayed.

AAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGC  >  NC_000913/4018967‑4019146

 aaTCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGa                                                                                        >  1:9900963/1‑93 (MQ=255)

 aaTCTGTTTAATACGGCGCGTAGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGa                                                                                        >  1:5614636/1‑93 (MQ=255)

     tGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGcc                                                                                    >  2:414066/1‑93 (MQ=255)

     tGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGcc                                                                                    >  2:2838937/1‑93 (MQ=255)

     tGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGcc                                                                                    <  1:9759033/93‑1 (MQ=255)

       tttAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAg                                                                                  >  1:5454841/1‑93 (MQ=255)

           aTACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGGGGGACGCCAGCttt                                                                              >  2:7322605/1‑93 (MQ=255)

            tACGGCGCGTCGCTTCAATATGGAAGGGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGGGGGGCGCCAGGTTTa                                                                             >  1:2160001/1‑93 (MQ=255)

              cGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATc                                                                           <  2:6645358/93‑1 (MQ=255)

              cGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATc                                                                           <  2:1566893/93‑1 (MQ=255)

              cGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATc                                                                           <  1:7814624/93‑1 (MQ=255)

              aggCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATc                                                                           <  1:2456159/92‑1 (MQ=255)

                 cgcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGc                                                                        <  1:1641833/93‑1 (MQ=255)

                 cgcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGc                                                                        >  2:3728070/1‑93 (MQ=255)

                  gcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCa                                                                       >  1:4815213/1‑93 (MQ=255)

                  gcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCa                                                                       >  1:2595685/1‑93 (MQ=255)

                   cgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCaa                                                                      >  2:9242027/1‑93 (MQ=255)

                   cgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCaa                                                                      >  1:4099113/1‑93 (MQ=255)

                     tCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACt                                                                    >  1:469596/1‑93 (MQ=255)

                     tCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACt                                                                    <  2:5247231/93‑1 (MQ=255)

                      cGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTc                                                                   <  1:10016683/93‑1 (MQ=255)

                       gCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCg                                                                  >  1:2779492/1‑93 (MQ=255)

                         ttCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGAt                                                                >  1:5939886/1‑93 (MQ=255)

                          tCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGAtt                                                               >  2:8434083/1‑93 (MQ=255)

                            aaTATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTa                                                             <  1:3016282/93‑1 (MQ=255)

                            aaTATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTa                                                             <  2:2025077/93‑1 (MQ=255)

                            aaTATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTa                                                             >  2:422966/1‑93 (MQ=255)

                             atatGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTAt                                                            <  2:5722442/93‑1 (MQ=255)

                              tatGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATg                                                           >  2:112520/1‑93 (MQ=255)

                              tatGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATg                                                           <  2:2749248/93‑1 (MQ=255)

                               atGGAAGGGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATga                                                          >  1:4986677/1‑92 (MQ=255)

                                tGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGa                                                         <  1:6471466/93‑1 (MQ=255)

                                tGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGa                                                         <  2:9847064/93‑1 (MQ=255)

                                 ggAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGaa                                                        <  1:5163561/93‑1 (MQ=255)

                                   aaGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGaaaa                                                      <  1:9989414/93‑1 (MQ=255)

                                   aaGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGaaaa                                                      >  1:3896793/1‑93 (MQ=255)

                                    aGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAAc                                                     <  2:5464023/93‑1 (MQ=255)

                                    aGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAAc                                                     <  1:801503/93‑1 (MQ=255)

                                      tGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACgg                                                   <  2:1367194/93‑1 (MQ=255)

                                      tGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACgg                                                   >  2:2655170/1‑93 (MQ=255)

                                        cAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCg                                                 >  1:7135042/1‑93 (MQ=255)

                                        cAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCg                                                 <  1:2287622/93‑1 (MQ=255)

                                          gCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGaa                                               <  2:3141375/93‑1 (MQ=255)

                                          gCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGaa                                               >  1:697919/1‑93 (MQ=255)

                                            cGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGc                                             <  1:2918831/93‑1 (MQ=255)

                                            cGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGc                                             <  1:9672881/93‑1 (MQ=255)

                                            cGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGc                                             <  2:8840093/93‑1 (MQ=255)

                                             gCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGcc                                            >  2:9090091/1‑93 (MQ=255)

                                              cAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCt                                           >  1:6680019/1‑93 (MQ=255)

                                               aaCTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCtt                                          <  2:6267536/93‑1 (MQ=255)

                                                aCTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCttt                                         <  1:6206713/93‑1 (MQ=255)

                                                  tGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTcc                                       <  2:7153816/93‑1 (MQ=255)

                                                  tGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTcc                                       <  2:6419212/93‑1 (MQ=255)

                                                   ggTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCt                                      >  1:8132236/1‑93 (MQ=255)

                                                   ggTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCt                                      >  1:3646097/1‑93 (MQ=255)

                                                     tgtTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTgg                                    <  1:6873149/93‑1 (MQ=255)

                                                      gtTACTGCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGa                                   <  1:9085615/93‑1 (MQ=255)

                                                      gtTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGa                                   <  2:175202/93‑1 (MQ=255)

                                                      gtTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGa                                   <  2:8995061/93‑1 (MQ=255)

                                                       ttACTCCAGAGAGCCCTGCTCTACGTCGAAGGGGTGGGTCGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAg                                  <  1:8411330/93‑1 (MQ=255)

                                                       ttACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAg                                  >  2:2100280/1‑93 (MQ=255)

                                                       ttACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAg                                  >  2:3317632/1‑93 (MQ=255)

                                                       ttACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAg                                  >  1:5393230/1‑93 (MQ=255)

                                                          cTCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCg                               <  1:8515795/93‑1 (MQ=255)

                                                           tCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCgg                              >  1:7484468/1‑92 (MQ=255)

                                                           tCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGt                              >  2:9452834/1‑93 (MQ=255)

                                                           tCCAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGGCgg                              >  1:4396670/1‑92 (MQ=255)

                                                            ccAGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTg                             <  2:5633040/93‑1 (MQ=255)

                                                              aGAAAACCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGa                           >  2:7639846/1‑93 (MQ=255)

                                                                aaaaCCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGAtt                         >  2:3877265/1‑93 (MQ=255)

                                                                 aaaCCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTa                        >  2:2326492/1‑93 (MQ=255)

                                                                   aCCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTaaa                      >  1:1348337/1‑93 (MQ=255)

                                                                   aCCCTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTaaa                      <  1:9545459/93‑1 (MQ=255)

                                                                     ccTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGa                    <  2:1298760/93‑1 (MQ=255)

                                                                      cTGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGAt                   <  1:3134957/93‑1 (MQ=255)

                                                                       tGCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTGCCTGGAGTCGTGGATTAAAGATc                  >  2:5534277/1‑93 (MQ=255)

                                                                        gCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCa                 <  2:96958/93‑1 (MQ=255)

                                                                        gCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCa                 <  1:6121572/93‑1 (MQ=255)

                                                                        gCTCTACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCa                 <  2:7939492/93‑1 (MQ=255)

                                                                          tctACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAgg               <  2:348045/93‑1 (MQ=255)

                                                                           ctACGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGt              <  1:1783510/93‑1 (MQ=255)

                                                                             aCGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCg            >  2:8282067/1‑93 (MQ=255)

                                                                             aCGTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCg            <  1:7263919/93‑1 (MQ=255)

                                                                             aCGTCGAAGGGGTGAGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCg            <  2:9925981/93‑1 (MQ=255)

                                                                               gTCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGt          >  1:5701091/1‑93 (MQ=255)

                                                                                tCGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTa         <  1:8822682/93‑1 (MQ=255)

                                                                                 cGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTAt        <  2:4604218/93‑1 (MQ=255)

                                                                                 cGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTAt        >  2:4540895/1‑93 (MQ=255)

                                                                                 cGAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCCGTAt        <  2:6492113/93‑1 (MQ=255)

                                                                                  gAAGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTAtt       <  2:1828247/93‑1 (MQ=255)

                                                                                   aaGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTc      >  2:4683734/1‑93 (MQ=255)

                                                                                    aGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTcc     <  2:4994920/93‑1 (MQ=255)

                                                                                    aGGGGTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTcc     <  1:1213021/93‑1 (MQ=255)

                                                                                     ggggTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCt    <  2:6983239/93‑1 (MQ=255)

                                                                                     ggggTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCt    <  2:6039034/93‑1 (MQ=255)

                                                                                      gggTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTg   >  2:5036823/1‑93 (MQ=255)

                                                                                      gggTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTg   <  1:933138/93‑1 (MQ=255)

                                                                                      gggTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTg   <  1:7685727/93‑1 (MQ=255)

                                                                                       ggTGGGACGCCTGCTTTATACGCATCTCGATTTGTGGACACCGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTgc  <  1:8950671/93‑1 (MQ=255)

                                                                                       ggTGGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTgc  >  1:2732415/1‑93 (MQ=255)

AAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGC  >  NC_000913/4018967‑4019146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`