BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,023,531	T→C	H318H (CAT→CAC)	yigC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,023,531	0	T	C	98.7%	1513.8 / 2.3	476	H318H (CAT→CAC)	yigC	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (1/5); new base C (277/193); total (278/198)
Fisher's exact test for biased strand distribution p-value = 8.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 485 total aligned reads displayed.

TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT  >  NC_000913/4023441‑4023620

tGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTc                                                                                         >  1:7659984/1‑93 (MQ=255)

tGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTc                                                                                         >  2:4595407/1‑93 (MQ=255)

   cGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCAc                                                                                      <  2:7506458/93‑1 (MQ=255)

     aCCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCt                                                                                    <  1:5509178/93‑1 (MQ=255)

     aCCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCt                                                                                    >  2:8914656/1‑93 (MQ=255)

       cacaCCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCtat                                                                                  >  2:3614906/1‑93 (MQ=255)

        acacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGCAGATGCGATTTACCACTCCACCtata                                                                                 >  2:7162883/1‑93 (MQ=255)

        acacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCtatc                                                                                 >  2:3111041/1‑92 (MQ=255)

         cacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAc                                                                                <  2:230867/93‑1 (MQ=255)

         cacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAc                                                                                >  1:9183966/1‑93 (MQ=255)

         cacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAc                                                                                >  2:6370854/1‑93 (MQ=255)

         cacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAc                                                                                >  1:4253511/1‑93 (MQ=255)

           ccGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGACGATGCGATTTACCACTCCACCTATACCg                                                                              >  1:241406/1‑93 (MQ=255)

            cGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCgg                                                                             >  1:4154059/1‑93 (MQ=255)

             ggTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCggg                                                                            >  1:5889801/1‑93 (MQ=255)

              gTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGc                                                                           >  1:1121703/1‑93 (MQ=255)

               ttACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCg                                                                          >  1:8349472/1‑93 (MQ=255)

               ttACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCg                                                                          <  2:3864794/93‑1 (MQ=255)

                tACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGt                                                                         >  2:1814090/1‑93 (MQ=255)

                  cTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTcc                                                                       <  2:1458654/93‑1 (MQ=255)

                   tataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCg                                                                      <  1:4644706/93‑1 (MQ=255)

                   tataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCg                                                                      >  2:515000/1‑93 (MQ=255)

                     taATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGcc                                                                    >  2:9288247/1‑93 (MQ=255)

                       aTGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAg                                                                  <  2:772495/93‑1 (MQ=255)

                        tGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGa                                                                 <  1:9937692/93‑1 (MQ=255)

                          aaGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               <  2:6814045/93‑1 (MQ=255)

                          aaGTCGATAATTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               <  1:6407930/93‑1 (MQ=255)

                           aGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGa                                                              >  2:4981509/1‑93 (MQ=255)

                             tCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGc                                                            >  2:8482234/1‑93 (MQ=255)

                             tCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGc                                                            <  2:7993856/93‑1 (MQ=255)

                             tCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGc                                                            >  1:1360410/1‑93 (MQ=255)

                               gATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGccc                                                          >  2:5391192/1‑93 (MQ=255)

                                aTAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcg                                                         >  2:3367567/1‑93 (MQ=255)

                                aTAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACAACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcg                                                         >  1:3014362/1‑93 (MQ=255)

                                 tAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcgc                                                        <  1:3974736/93‑1 (MQ=255)

                                 tAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcgc                                                        <  2:8724238/93‑1 (MQ=255)

                                   gTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCgg                                                      >  2:2165488/1‑93 (MQ=255)

                                    tttCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGt                                                     >  1:5190218/1‑93 (MQ=255)

                                     ttCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTg                                                    >  1:1777557/1‑93 (MQ=255)

                                       ccttgTATTTCCCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCt                                                  <  2:1942536/89‑1 (MQ=255)

                                        ccGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTg                                                 <  2:704340/93‑1 (MQ=255)

                                         cGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTgg                                                >  1:8307505/1‑93 (MQ=255)

                                          ggTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTggg                                               >  2:5706764/1‑93 (MQ=255)

                                          ggTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTggg                                               <  2:4880289/93‑1 (MQ=255)

                                           gTATTTCCCGTGACGCATATTACCCAGCGTGAAGATCCGATTTACCACTCCGCCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              <  2:4011054/93‑1 (MQ=255)

                                           gTATTTACCGTGACGCATATTACTCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              <  2:5535391/93‑1 (MQ=255)

                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              >  1:8172201/1‑93 (MQ=255)

                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              >  2:1264270/1‑93 (MQ=255)

                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              <  1:7328745/93‑1 (MQ=255)

                                            tATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtg                                             >  2:740778/1‑93 (MQ=255)

                                            tATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtg                                             >  1:165954/1‑93 (MQ=255)

                                            tATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtg                                             >  1:966732/1‑93 (MQ=255)

                                             aTTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtgt                                            <  2:9127171/93‑1 (MQ=255)

                                             aTTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtgt                                            <  1:6919767/93‑1 (MQ=255)

                                              tttACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTc                                           >  1:6582899/1‑93 (MQ=255)

                                                 aCCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCa                                        <  1:6117590/93‑1 (MQ=255)

                                                  ccGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCAc                                       >  1:7125941/1‑93 (MQ=255)

                                                     tGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGa                                    <  1:6278951/93‑1 (MQ=255)

                                                     tGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGa                                    <  1:8845727/93‑1 (MQ=255)

                                                        cGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACg                                 >  2:4782793/1‑93 (MQ=255)

                                                         gCATATTACCCAGCGTGAAGATGCTATTCACCACCCCACCTATCCCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGa                                <  1:4969863/93‑1 (MQ=255)

                                                         gCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGa                                <  2:3718176/93‑1 (MQ=255)

                                                          cATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGaa                               <  2:3487088/93‑1 (MQ=255)

                                                           atatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAg                              >  2:6037549/1‑93 (MQ=255)

                                                           atatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAg                              <  1:2650233/93‑1 (MQ=255)

                                                            tatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgt                             >  1:3207803/1‑93 (MQ=255)

                                                            tatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgt                             >  2:6511903/1‑93 (MQ=255)

                                                             atTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgtg                            <  2:8265919/93‑1 (MQ=255)

                                                             atTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgtg                            >  2:8623096/1‑93 (MQ=255)

                                                               tACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGtt                          <  2:9575511/93‑1 (MQ=255)

                                                                aCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGttt                         >  2:4158225/1‑93 (MQ=255)

                                                                aCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGttt                         >  2:2558510/1‑93 (MQ=255)

                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  2:810392/1‑93 (MQ=255)

                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       <  2:9428600/93‑1 (MQ=255)

                                                                    aGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGc                     <  1:7997905/93‑1 (MQ=255)

                                                                     gCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGcg                    >  2:2891742/1‑92 (MQ=255)

                                                                     gCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGcc                    <  2:1735013/93‑1 (MQ=255)

                                                                      cGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCg                   >  2:784146/1‑93 (MQ=255)

                                                                       gTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGa                  >  2:5257894/1‑93 (MQ=255)

                                                                       gTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGa                  <  1:9340240/93‑1 (MQ=255)

                                                                        tGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 <  2:8349471/93‑1 (MQ=255)

                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 <  2:5072054/93‑1 (MQ=255)

                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 >  1:5769245/1‑93 (MQ=255)

                                                                         gAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAtt                <  1:3823031/93‑1 (MQ=255)

                                                                         gAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAtt                <  2:6605813/93‑1 (MQ=255)

                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               >  1:7882445/1‑93 (MQ=255)

                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               >  1:10005579/1‑93 (MQ=255)

                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               <  1:4701375/93‑1 (MQ=255)

                                                                           aGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCt              <  1:198215/93‑1 (MQ=255)

                                                                            gATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGCTTCTg             >  1:155313/1‑93 (MQ=255)

                                                                             aTGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGc            >  1:3479676/1‑93 (MQ=255)

                                                                             aTGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGc            <  1:2197687/93‑1 (MQ=255)

                                                                              tGCGATTTACCACTCCACCTATAACGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGCAGTGTTTGTGACGATTCTGCa           >  1:370136/1‑93 (MQ=255)

                                                                               gCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaa          >  1:359370/1‑93 (MQ=255)

                                                                                cGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaa         <  2:8793288/93‑1 (MQ=255)

                                                                                 gATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaaa        >  1:981059/1‑93 (MQ=255)

                                                                                  aTTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaaaa       <  1:9510328/93‑1 (MQ=255)

                                                                                   tttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAc      >  1:8566992/1‑93 (MQ=255)

                                                                                   tttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAc      <  1:5079280/93‑1 (MQ=255)

                                                                                    ttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAcaca     >  1:4457475/1‑93 (MQ=255)

TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT  >  NC_000913/4023441‑4023620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`