BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,390	C→T	A65V (GCG→GTG)	hemG →	protoporphyrin oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,390	0	C	T	99.8%	1793.8 / ‑5.9	488	A65V (GCG→GTG)	hemG	protoporphyrin oxidase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (243/244); total (244/244)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01

Only 100 of 504 total aligned reads displayed.

CCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGG  >  NC_000913/4032300‑4032479

ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                                                                                         >  2:8363232/1‑93 (MQ=255)

ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                                                                                         <  2:623139/93‑1 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                       <  2:3310135/93‑1 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                       <  2:2256594/93‑1 (MQ=255)

   aTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTcc                                                                                      <  1:4241259/93‑1 (MQ=255)

    tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                                                                                     <  2:3521493/93‑1 (MQ=255)

     gtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAg                                                                                    <  2:1683526/93‑1 (MQ=255)

      tgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAgg                                                                                   >  1:6762317/1‑93 (MQ=255)

        cACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGaa                                                                                 <  1:9965034/93‑1 (MQ=255)

         aCCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAt                                                                                >  1:6575825/1‑93 (MQ=255)

          ccGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAtt                                                                               <  1:919656/93‑1 (MQ=255)

          ccGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAtt                                                                               >  2:1822942/1‑93 (MQ=255)

          ccGCAGTTAAGAACCACGGTGGGAAAACTATGGCCGTGGGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCATTGTTCCAGGAAtt                                                                               <  2:7546076/93‑1 (MQ=255)

            gCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTg                                                                             <  1:1610092/93‑1 (MQ=255)

             cATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGt                                                                            <  1:8503023/93‑1 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                          >  2:8893945/1‑93 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                          >  1:9600259/1‑93 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                          >  2:6489044/1‑93 (MQ=255)

                tGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaa                                                                         <  2:6278067/93‑1 (MQ=255)

                tGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaa                                                                         >  2:8224687/1‑93 (MQ=255)

                  aagaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaa                                                                       <  2:9776341/93‑1 (MQ=255)

                     aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAc                                                                    <  2:5173180/93‑1 (MQ=255)

                       ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt                                                                  >  2:4908225/1‑93 (MQ=255)

                       ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt                                                                  <  1:7497140/93‑1 (MQ=255)

                         acggTGGGAAAACTATGACCGTTTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGc                                                                >  1:1797825/4‑93 (MQ=255)

                         acggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGc                                                                <  2:1543616/90‑1 (MQ=255)

                          cggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCg                                                               >  1:316804/3‑93 (MQ=255)

                           ggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGa                                                              <  1:7821709/92‑1 (MQ=255)

                            gTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAc                                                             <  2:3815563/93‑1 (MQ=255)

                            gTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAc                                                             >  2:9332761/1‑93 (MQ=255)

                             tGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcg                                                            >  2:3664553/1‑93 (MQ=255)

                             tGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcg                                                            >  1:2375167/1‑93 (MQ=255)

                              gggAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcgc                                                           <  1:702439/93‑1 (MQ=255)

                               ggAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcgcg                                                          <  1:4758418/93‑1 (MQ=255)

                                gAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCgg                                                         >  1:373982/1‑93 (MQ=255)

                                  aaaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCt                                                       >  1:4057884/1‑93 (MQ=255)

                                     cTATGTCCGTGTTGTCATTGGTGCTTCTATTCGCTATGGTCACTTCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGaa                                                    <  1:2745587/93‑1 (MQ=255)

                                     cTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGaa                                                    <  1:3016710/93‑1 (MQ=255)

                                      tATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAt                                                   >  1:5337774/1‑93 (MQ=255)

                                      tATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAt                                                   <  2:6011625/93‑1 (MQ=255)

                                       aTGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAtt                                                  <  1:1395315/93‑1 (MQ=255)

                                         gACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCg                                                <  1:7293074/93‑1 (MQ=255)

                                          gccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                               <  1:8879218/92‑1 (MQ=255)

                                          gccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                               <  1:7641761/92‑1 (MQ=255)

                                          gccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACACGCGACGCGGCTGAATTCGa                                               <  1:9740793/92‑1 (MQ=255)

                                          aCCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                               <  1:575189/93‑1 (MQ=255)

                                          aCCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                               >  1:2012571/1‑93 (MQ=255)

                                           ccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGAt                                              <  2:544140/93‑1 (MQ=255)

                                            cGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAACAAACATGCGACGCGGCTGAATTCGAtc                                             >  2:9183558/1‑92 (MQ=255)

                                            cGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATg                                             <  2:5781441/93‑1 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                            <  1:8692845/93‑1 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                            >  2:1856004/1‑93 (MQ=255)

                                                    cATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAgcgc                                     >  1:8365910/1‑93 (MQ=255)

                                                      gtGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCt                                   <  2:2722128/92‑1 (MQ=255)

                                                        ggTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCttt                                 >  2:4632129/1‑93 (MQ=255)

                                                          tGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTa                               <  2:1607821/93‑1 (MQ=255)

                                                          tGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTa                               <  2:4834172/93‑1 (MQ=255)

                                                           gCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTAc                              <  1:7967772/93‑1 (MQ=255)

                                                              tCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTcc                           <  1:73884/93‑1 (MQ=255)

                                                              tCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTcc                           >  1:6153512/1‑93 (MQ=255)

                                                                tATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                         >  2:8750546/1‑93 (MQ=255)

                                                                tATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                         >  2:7752571/1‑93 (MQ=255)

                                                                tATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                         >  2:9468274/1‑93 (MQ=255)

                                                                tATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                         <  1:6026225/93‑1 (MQ=255)

                                                                tATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                         <  2:2007361/93‑1 (MQ=255)

                                                                 aTTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTg                        >  2:2876541/1‑93 (MQ=255)

                                                                  ttCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGa                       >  2:4005229/1‑93 (MQ=255)

                                                                   tCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGaa                      <  1:2553507/93‑1 (MQ=255)

                                                                   tCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGaa                      <  2:9587959/93‑1 (MQ=255)

                                                                     gCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATc                    <  2:6965210/93‑1 (MQ=255)

                                                                     gCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATc                    <  2:1247808/93‑1 (MQ=255)

                                                                     gCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATc                    >  1:2903818/1‑93 (MQ=255)

                                                                       tATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtg                  >  1:5102559/1‑93 (MQ=255)

                                                                        aTGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtgg                 <  2:3009575/93‑1 (MQ=255)

                                                                        aTGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtgg                 >  2:7145520/1‑93 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCTGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt                <  2:7225412/93‑1 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt                <  1:2289612/93‑1 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt                <  2:4445450/93‑1 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt                <  1:6378332/93‑1 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt                <  2:8432084/93‑1 (MQ=255)

                                                                          ggTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggtg               >  1:3416975/1‑93 (MQ=255)

                                                                           gTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggtgg              >  1:9288478/1‑93 (MQ=255)

                                                                            tCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgc             <  2:3429613/93‑1 (MQ=255)

                                                                            tCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgc             <  2:1124887/93‑1 (MQ=255)

                                                                             cACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcg            >  1:4476944/1‑93 (MQ=255)

                                                                             cACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcg            <  2:2390344/93‑1 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc           >  1:8204751/1‑93 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc           >  1:9854397/1‑93 (MQ=255)

                                                                                tACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcgc         >  1:1138139/1‑93 (MQ=255)

                                                                                tACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcgc         >  2:7647415/1‑93 (MQ=255)

                                                                                tACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcgc         >  1:7164572/1‑93 (MQ=255)

                                                                                 aCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa        >  2:1326235/1‑93 (MQ=255)

                                                                                 aCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa        >  1:1035450/1‑93 (MQ=255)

                                                                                   cATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaaa      <  2:8553800/93‑1 (MQ=255)

                                                                                   cATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaaa      <  1:4889252/93‑1 (MQ=255)

                                                                                    aTTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc     >  2:6580340/1‑93 (MQ=255)

                                                                                    aTTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc     >  2:4293977/1‑93 (MQ=255)

                                                                                    aTTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc     >  2:4201365/1‑93 (MQ=255)

                                                                                      tCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg   >  1:5585139/1‑93 (MQ=255)

                                                                                      tCAGTGTGCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg   <  2:2558185/93‑1 (MQ=255)

CCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGG  >  NC_000913/4032300‑4032479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`