BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,011	C→T	A199A (GCC→GCT)	metL →	aspartokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,011	0	C	T	99.6%	1005.2 / ‑5.6	274	A199A (GCC→GCT)	metL	aspartokinase II
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (131/142); total (132/142)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

Only 100 of 288 total aligned reads displayed.

AGGGCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCCGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTG  >  NC_000913/4127921‑4128100

 gggCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGt                                                                                        <  2:2355569/93‑1 (MQ=255)

 gggCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGt                                                                                        <  1:8329024/93‑1 (MQ=255)

 gggCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGt                                                                                        >  2:5380840/1‑93 (MQ=255)

 gggCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGt                                                                                        >  2:60160/1‑93 (MQ=255)

  ggCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTg                                                                                       >  1:3190876/1‑93 (MQ=255)

     tttctttcCCGTTGGTGTAACAGCTGCTGGTGCAACATCCGCGCAAAGGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGaaa                                                                                    <  1:9543532/93‑1 (MQ=255)

     tttCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGaaa                                                                                    <  1:9643250/93‑1 (MQ=255)

     tttCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGaaa                                                                                    <  2:8750821/93‑1 (MQ=255)

     tttCTTACCCGTTGCTGCAACAGCTGCTGGTGCAAAATCCGGGCAAACGTCTGGTGGTGACCGGCTTTATCAGCCGCAAAAACGCTTGTGaaa                                                                                    >  1:5094011/1‑93 (MQ=255)

     tttCTTACCCGTGGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGaaa                                                                                    <  2:961169/93‑1 (MQ=255)

      ttCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAAc                                                                                   >  1:8104001/1‑93 (MQ=255)

       tCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACg                                                                                  <  2:8345105/93‑1 (MQ=255)

         ttACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGt                                                                                <  2:4882872/93‑1 (MQ=255)

           ccccGTTGCTGCAACAGCTGCTGGTGCATCATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGtgc                                                                              <  1:351660/92‑1 (MQ=255)

             ccGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGtgctg                                                                            <  2:979772/93‑1 (MQ=255)

             ccGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGtgctg                                                                            >  2:5634836/1‑93 (MQ=255)

              cGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGtgctgc                                                                           <  1:6539652/93‑1 (MQ=255)

              cGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGtgctgc                                                                           <  1:5861897/93‑1 (MQ=255)

              cGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGtgctgc                                                                           <  1:5509908/93‑1 (MQ=255)

                ttGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGctgctg                                                                         <  2:242270/93‑1 (MQ=255)

                  gctgcAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTggg                                                                       <  2:9390877/93‑1 (MQ=255)

                     gcAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCg                                                                    <  1:9975170/93‑1 (MQ=255)

                        aCAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTaa                                                                 <  2:4527932/93‑1 (MQ=255)

                         cAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAAc                                                                <  1:3737185/93‑1 (MQ=255)

                         cAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAAc                                                                >  2:3523971/1‑93 (MQ=255)

                         cAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAAc                                                                <  1:7721198/93‑1 (MQ=255)

                          aGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACg                                                               >  2:3889452/1‑93 (MQ=255)

                          aGCTGCTGGTGAAACATCCGGGCAAACGTCTGGTGGTGCCCTGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACg                                                               <  1:4403586/93‑1 (MQ=255)

                           gctgctGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGTTGCTGCTGGGGCGTAACgg                                                              <  1:472424/93‑1 (MQ=255)

                           gctgctGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACgg                                                              >  2:369086/1‑93 (MQ=255)

                           gctgctGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACgg                                                              <  1:369404/93‑1 (MQ=255)

                               cttgTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTcc                                                          <  2:4664629/90‑1 (MQ=255)

                               ctgGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTcc                                                          <  1:8898958/93‑1 (MQ=255)

                               ctgGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTcc                                                          <  1:3974996/93‑1 (MQ=255)

                                 ggTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGa                                                        <  1:8304587/93‑1 (MQ=255)

                                 ggTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGa                                                        >  1:4141838/1‑93 (MQ=255)

                                   tGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGAAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCCACt                                                      >  1:2934814/1‑93 (MQ=255)

                                    gCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACtg                                                     <  1:8357836/93‑2 (MQ=255)

                                    gCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTa                                                     <  1:377736/93‑1 (MQ=255)

                                     caccATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTAt                                                    <  2:1427857/90‑1 (MQ=255)

                                      aaCATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTAtt                                                   <  1:7133154/93‑1 (MQ=255)

                                      aaCATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTAtt                                                   <  2:3009357/93‑1 (MQ=255)

                                      aaCATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTAtt                                                   <  2:2407649/93‑1 (MQ=255)

                                       aCATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTc                                                  >  2:5738742/1‑93 (MQ=255)

                                        cATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTcc                                                 <  1:2444618/93‑1 (MQ=255)

                                         aTCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCcg                                                <  1:9013547/93‑1 (MQ=255)

                                         aTCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCcg                                                <  2:7433042/93‑1 (MQ=255)

                                          tCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCcgc                                               >  1:1931904/1‑93 (MQ=255)

                                          tCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCcgc                                               >  1:1626007/1‑93 (MQ=255)

                                            cGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGa                                             >  1:9676495/1‑93 (MQ=255)

                                            cGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGa                                             <  2:8259168/93‑1 (MQ=255)

                                              ggCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGaca                                           >  1:5194323/1‑93 (MQ=255)

                                              ggCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGaca                                           <  1:217701/93‑1 (MQ=255)

                                               gCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGacac                                          <  1:2687979/93‑1 (MQ=255)

                                                  aaCGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACaaa                                       <  2:1861131/93‑1 (MQ=255)

                                                  aaCGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACaaa                                       >  1:335837/1‑93 (MQ=255)

                                                  aaCGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACaaa                                       <  2:7142020/93‑1 (MQ=255)

                                                   aCGTCGGGTGGTGACCGGATTGAGCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAAt                                      <  1:1494767/93‑1 (MQ=255)

                                                    cGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATc                                     <  2:2552031/93‑1 (MQ=255)

                                                    cGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATc                                     <  2:503462/93‑1 (MQ=255)

                                                     gTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCg                                    >  1:2073335/1‑93 (MQ=255)

                                                     gTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCg                                    <  1:4875089/93‑1 (MQ=255)

                                                      tCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCgg                                   >  2:4063038/1‑93 (MQ=255)

                                                      tCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCgg                                   >  1:9820868/1‑93 (MQ=255)

                                                        tggtggTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTg                                 >  2:6486526/1‑93 (MQ=255)

                                                        tggtggTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTg                                 >  2:1601985/1‑93 (MQ=255)

                                                        tggtggTGACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTg                                 <  1:9343736/93‑1 (MQ=255)

                                                        tggtggTGACCAGATCTATCAGCCGCAAGAACGCTGGTGAACCGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACCCAAATCGGTg                                 <  2:8504260/93‑1 (MQ=255)

                                                          gtggtgACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTgcg                               >  2:326778/1‑93 (MQ=255)

                                                          gtggtgACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTgcg                               <  1:8827785/93‑1 (MQ=255)

                                                            ggtgACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCt                             <  1:4099818/93‑1 (MQ=255)

                                                              tgcccGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTgg                           <  2:86878/90‑1 (MQ=255)

                                                              tgACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTgg                           <  2:1273562/93‑1 (MQ=255)

                                                              tgACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTgg                           >  2:6368419/1‑93 (MQ=255)

                                                              tgACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGCGCGCAACGGATCGGACTATTCCCCGTCACTAATCGGTGCGCTgg                           >  2:6111320/1‑93 (MQ=255)

                                                               gACCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGc                          >  1:1130212/1‑93 (MQ=255)

                                                                aCCGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCg                         >  1:7274481/1‑93 (MQ=255)

                                                                 ccGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTCGGCTGgggg                        >  2:5888183/1‑93 (MQ=255)

                                                                  cGGATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGcgggcggg                       >  2:2810336/1‑93 (MQ=255)

                                                                   ggATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGgt                      <  2:7238263/93‑1 (MQ=255)

                                                                    gATTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGgtg                     >  2:6775585/1‑93 (MQ=255)

                                                                    gATTTACCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGgtg                     <  2:9204063/93‑1 (MQ=255)

                                                                     aTTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGgtgt                    <  2:2710307/93‑1 (MQ=255)

                                                                     aTTTATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGgtgt                    >  1:3905312/1‑93 (MQ=255)

                                                                      tttATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGtt                   <  1:2350715/93‑1 (MQ=255)

                                                                      tttATCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGtt                   >  1:2671603/1‑93 (MQ=255)

                                                                          tCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTtctc               <  1:6952697/93‑1 (MQ=255)

                                                                          tCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTtctc               <  2:8131509/93‑1 (MQ=255)

                                                                          tCAGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTtctc               >  1:7980014/1‑93 (MQ=255)

                                                                            aGCCGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGGGTTTCTcgc             >  1:5683468/1‑93 (MQ=255)

                                                                              ccGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGt           >  2:6897333/1‑93 (MQ=255)

                                                                               cGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTa          <  2:833490/93‑1 (MQ=255)

                                                                               cGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTa          <  1:6493732/93‑1 (MQ=255)

                                                                               cGCAACAACGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGGGTTTCTcgcgga          >  2:2163792/1‑91 (MQ=255)

                                                                                 caacaaCGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAAc        >  2:4020136/1‑93 (MQ=255)

                                                                                  aacaacGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAAcc       <  1:2473256/93‑1 (MQ=255)

                                                                                  aacaacGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGGGTTTCTCGCGTAAcc       >  2:8857135/1‑93 (MQ=255)

                                                                                   acaacGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCa      >  1:10033331/1‑93 (MQ=255)

                                                                                      acGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCt   <  1:2495458/93‑1 (MQ=255)

                                                                                      acGCTGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCt   <  2:3376493/93‑1 (MQ=255)

AGGGCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACGTCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCCGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTG  >  NC_000913/4127921‑4128100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`