BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,651	A→G	L427L (TTA→TTG)	ybbX →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,651	0	A	G	99.8%	1468.9 / ‑5.1	449	L427L (TTA→TTG)	ybbX	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (214/234); total (215/234)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 460 total aligned reads displayed.

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

tGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCg                                                                                         <  1:6095838/93‑1 (MQ=255)

   tCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtgg                                                                                      <  1:8485821/93‑1 (MQ=255)

   tCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtgg                                                                                      <  1:1947778/93‑1 (MQ=255)

    cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggt                                                                                     >  1:9858925/1‑93 (MQ=255)

      tACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtga                                                                                   <  1:5299114/93‑1 (MQ=255)

       aCCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgat                                                                                  <  2:3364996/93‑1 (MQ=255)

       aCCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgat                                                                                  <  1:5337250/93‑1 (MQ=255)

        ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                 <  1:2420162/93‑1 (MQ=255)

        ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                 <  2:8009087/93‑1 (MQ=255)

         cAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgt                                                                                >  2:2230829/1‑93 (MQ=255)

          aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                               >  1:10008449/1‑93 (MQ=255)

          aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtcatgtg                                                                               >  1:4532304/1‑93 (MQ=255)

              aCGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAttt                                                                           <  1:5067663/93‑1 (MQ=255)

                 aTCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACg                                                                        >  1:4762317/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  2:3044589/1‑93 (MQ=255)

                    tGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACa                                                                     >  1:6839523/1‑93 (MQ=255)

                     ggAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                    <  1:6983138/93‑1 (MQ=255)

                      gAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAtt                                                                   >  1:2225249/1‑93 (MQ=255)

                      gAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAtt                                                                   <  2:7873816/93‑1 (MQ=255)

                        atatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGa                                                                 >  1:6572513/1‑93 (MQ=255)

                         tatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGaa                                                                <  1:4471181/93‑1 (MQ=255)

                          atCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAc                                                               >  2:5698984/1‑93 (MQ=255)

                           tCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACa                                                              <  1:8283802/93‑1 (MQ=255)

                           tCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGGGATGTGATTTACGACATTGAACa                                                              >  1:1217473/1‑93 (MQ=255)

                            cGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAg                                                             <  2:4101065/93‑1 (MQ=255)

                              ccACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAggg                                                           >  2:183/1‑93 (MQ=255)

                              ccACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAggg                                                           >  2:4498213/1‑93 (MQ=255)

                              ccACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAggg                                                           <  2:5243250/93‑1 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          >  1:9313782/1‑93 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          <  2:906450/93‑1 (MQ=255)

                                acaACGGCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCt                                                         <  2:632315/93‑1 (MQ=255)

                                 caAAGTGAGCCCGTATGATGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTAATGTGATTTACGGCATTGAACAGGGCtt                                                        <  2:824595/93‑1 (MQ=255)

                                 caAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCtt                                                        <  1:2757845/93‑1 (MQ=255)

                                  aaaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTc                                                       <  1:3167413/93‑1 (MQ=255)

                                   aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                      <  2:5926710/93‑1 (MQ=255)

                                    aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTccc                                                     <  1:7632665/93‑1 (MQ=255)

                                    aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTccc                                                     <  1:6428802/93‑1 (MQ=255)

                                     gTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCt                                                    >  2:8918349/1‑93 (MQ=255)

                                       cAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGt                                                  <  1:1271650/93‑1 (MQ=255)

                                        aGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGtt                                                 <  2:8691453/93‑1 (MQ=255)

                                        aGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGAAATTGAACAGGGCTTCCCtcat                                                 >  1:6235920/1‑90 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                >  2:7031389/1‑93 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                >  2:3710220/1‑93 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                <  2:2447048/93‑1 (MQ=255)

                                          cccGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgc                                               <  1:2503289/93‑1 (MQ=255)

                                            cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                             <  1:3619518/93‑1 (MQ=255)

                                             gTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGcc                                            >  1:161522/1‑93 (MQ=255)

                                              tATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCg                                           >  2:9801977/1‑93 (MQ=255)

                                              tATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCg                                           >  1:2737420/1‑93 (MQ=255)

                                                tGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaa                                         >  1:1110944/1‑93 (MQ=255)

                                                 gTTGGCCGTACCATTGGCGCGCGTATCACGAAACCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaac                                        <  2:6161688/93‑2 (MQ=255)

                                                   tGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAgg                                      <  2:6094661/93‑1 (MQ=255)

                                                      ccGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATGGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCa                                   <  2:8885261/93‑1 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 >  1:977364/1‑93 (MQ=255)

                                                         tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAtt                                >  2:8519861/1‑93 (MQ=255)

                                                         tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAtt                                >  2:313930/1‑93 (MQ=255)

                                                          aCCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAttt                               >  2:5762319/1‑93 (MQ=255)

                                                          aCCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAttt                               <  1:3834525/93‑1 (MQ=255)

                                                            cATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTAt                             <  1:1724063/93‑1 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           <  1:5772727/93‑1 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           >  2:5096878/1‑93 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           >  2:6637081/1‑93 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           >  1:4667965/1‑93 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           >  1:9041572/1‑93 (MQ=255)

                                                               tGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCt                          >  1:7732529/1‑93 (MQ=255)

                                                               tGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCt                          <  1:8842458/93‑1 (MQ=255)

                                                                ggCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCtt                         <  1:29646/93‑1 (MQ=255)

                                                                ggCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCtt                         >  2:2063521/1‑93 (MQ=255)

                                                                 gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                        >  1:6398343/1‑93 (MQ=255)

                                                                 gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                        <  1:3474059/93‑1 (MQ=255)

                                                                   gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                      >  2:712627/1‑93 (MQ=255)

                                                                   gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                      <  1:916604/93‑1 (MQ=255)

                                                                     gcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACa                    <  2:731119/93‑1 (MQ=255)

                                                                        tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                 >  2:5417622/1‑93 (MQ=255)

                                                                        tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                 >  1:9595321/1‑93 (MQ=255)

                                                                         aTCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcag                <  2:9520923/93‑1 (MQ=255)

                                                                         aTCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcag                <  1:8085635/93‑1 (MQ=255)

                                                                          tCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagc               >  1:4923582/1‑93 (MQ=255)

                                                                          tCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagc               >  1:406280/1‑93 (MQ=255)

                                                                           cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca              <  2:7405055/93‑1 (MQ=255)

                                                                            aCGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagcag             <  2:4883261/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          <  2:1266728/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          <  2:131913/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          >  2:9284754/1‑93 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         <  1:7359094/93‑1 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         <  1:8689143/93‑1 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         >  2:9614793/1‑93 (MQ=255)

                                                                                 aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc        <  1:328012/93‑1 (MQ=255)

                                                                                 aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc        <  2:3862798/93‑1 (MQ=255)

                                                                                 aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc        >  1:5495376/1‑93 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg      <  2:6920223/93‑1 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAAATCAGCAGTAATCTg      <  2:4288606/93‑1 (MQ=255)

                                                                                    cATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGCAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTgg     <  2:684723/93‑1 (MQ=255)

                                                                                    cATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTgg     <  2:9899702/93‑1 (MQ=255)

                                                                                    cATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTgg     >  2:1005104/1‑93 (MQ=255)

                                                                                    cATCTTGCGGGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCtca     >  2:1570909/1‑91 (MQ=255)

                                                                                     aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc    >  2:7610398/1‑93 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   <  1:3945287/93‑1 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   >  2:3543446/1‑93 (MQ=255)

                                                                                       cTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGccc  >  2:4588381/1‑93 (MQ=255)

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`