BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,295	A→G	V219A (GTA→GCA)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,295	0	A	G	99.4%	1010.0 / ‑5.1	316	V219A (GTA→GCA)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (170/142); total (170/146)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 345 total aligned reads displayed.

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

tGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTgcc                                                                                         >  2:6457901/1‑93 (MQ=255)

 ggTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTgcca                                                                                        >  1:1018756/1‑93 (MQ=255)

   tGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTgccacc                                                                                      >  2:3663785/1‑93 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  1:8343526/1‑93 (MQ=255)

      tGATCGCTCGCTTCCCACACCGGGTCGTCCCCCCACAGGTCGCCCCCTTCCTG‑CCAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   <  1:8979182/93‑1 (MQ=255)

       gATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAt                                                                                  >  1:525516/1‑93 (MQ=255)

       gATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAt                                                                                  >  2:6252064/1‑93 (MQ=255)

       gATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAt                                                                                  >  1:8747604/1‑93 (MQ=255)

        aTCGCTCGCTTGCTCACCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATg                                                                                 <  1:1477604/93‑1 (MQ=255)

        aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATg                                                                                 >  1:3938122/1‑93 (MQ=255)

           ccccgctTGCTCAACCAGGTCGTGCCCCCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctc                                                                              <  2:2960545/90‑1 (MQ=255)

            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                             >  2:9481167/1‑93 (MQ=255)

            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                             >  2:8475348/1‑93 (MQ=255)

             tcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTa                                                                            >  1:1606278/1‑93 (MQ=255)

               gctTGCTCAACCAGGTCGTGCCCCCACAGGCCGCGCCCTTCCTG‑CCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACg                                                                          <  2:7199237/93‑1 (MQ=255)

               gctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACg                                                                          >  1:5599622/1‑93 (MQ=255)

                ctgccTCACCCAGGTCGGGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGc                                                                         <  1:7906067/89‑1 (MQ=255)

                  tGCTCACCCAGGTCGTGCCCCCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTg                                                                       <  2:4438739/93‑1 (MQ=255)

                  tGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTg                                                                       >  2:5457636/1‑93 (MQ=255)

                  tGCCCAACCAGGTCGTGCCACCACAGGTCGCGCCCTCCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTg                                                                       <  1:6353458/93‑1 (MQ=255)

                    cTCACCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                     <  1:4426013/93‑1 (MQ=255)

                      cAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTgc                                                                   >  1:6887031/1‑92 (MQ=255)

                      cAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGa                                                                   >  1:7644370/1‑93 (MQ=255)

                       acccAGGGCGTCCCCCCACAGGTCGCCCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGAc                                                                  <  2:1892226/91‑1 (MQ=255)

                          cAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               >  1:4814068/1‑93 (MQ=255)

                          cAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               >  1:4281659/1‑93 (MQ=255)

                           aGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTc                                                              <  1:3049616/93‑1 (MQ=255)

                            ggTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCg                                                             >  1:8177388/1‑93 (MQ=255)

                            ggTCGTCCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCg                                                             <  1:9673663/93‑1 (MQ=255)

                             gTCGTGCCACCACGGGTCGCCCCTTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCgg                                                            <  1:1977101/93‑1 (MQ=255)

                             gTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCgg                                                            >  1:7312648/1‑93 (MQ=255)

                                gTGCCCCCACGGGCCGCGCCCTTCCTG‑CCAGACAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         <  2:9120545/93‑1 (MQ=255)

                                gTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCAGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         >  1:1057235/1‑93 (MQ=255)

                                    caccacAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTc                                                     >  1:5557610/1‑93 (MQ=255)

                                      ccacAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAg                                                   >  2:6360928/1‑93 (MQ=255)

                                      ccacAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAg                                                   >  2:8353434/1‑93 (MQ=255)

                                       cacaGGTCGCCCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgc                                                  <  1:3647176/93‑1 (MQ=255)

                                        acaGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 >  1:1206451/1‑93 (MQ=255)

                                           ggTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGt                                              >  2:3721755/1‑93 (MQ=255)

                                                cgcCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatca                                         >  2:1192799/1‑93 (MQ=255)

                                                cgcCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatca                                         >  2:8033292/1‑93 (MQ=255)

                                                 gcCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        >  2:4638034/1‑93 (MQ=255)

                                                 ccccTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        <  2:5240225/92‑1 (MQ=255)

                                                  cccTTCCCGCCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        <  1:6015790/93‑1 (MQ=255)

                                                   ccTCCCTG‑CCAGTCAATTCTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGTTTTTTCAGCGCGTCATCAACg                                      <  2:4779547/93‑1 (MQ=255)

                                                    ctgccTG‑CCAGTTAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGt                                     <  2:2340861/90‑1 (MQ=255)

                                                    cTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCCTCCACGt                                     >  2:7765112/1‑93 (MQ=255)

                                                    cTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGt                                     >  2:7339272/1‑93 (MQ=255)

                                                    cTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGt                                     >  1:7058288/1‑93 (MQ=255)

                                                     ttCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    >  2:9195132/1‑93 (MQ=255)

                                                     ttCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    >  2:7701053/1‑93 (MQ=255)

                                                     tgccTG‑CCAGTCATTTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGATTTTTCAGCGCGTCATCAACGtt                                    <  2:7439814/91‑1 (MQ=255)

                                                       ccTG‑CCAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                  <  1:278113/93‑1 (MQ=255)

                                                       ccTG‑CCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                  <  2:6638867/93‑1 (MQ=255)

                                                         tG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACCTGCTCTACGCTGGTGACGTTCGGGGTTTTCAGCGCGTCATCAACGtgtttt                                >  2:2662431/1‑92 (MQ=255)

                                                          g‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGCCATCAACGTTTttct                               >  1:8125261/1‑93 (MQ=255)

                                                            ccAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                              <  2:4292046/93‑1 (MQ=255)

                                                              aGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            <  2:5679574/93‑1 (MQ=255)

                                                              aGTCAATTTTCCCGCCAGTCCGCTTCAGTGCCACCACATGCTCTAAGCTGGTGACGTTCGGGTTTTTCCGCTCGTCATCAACGTTTTTCTTCa                            >  2:219271/1‑93 (MQ=255)

                                                              aGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            <  2:6048138/93‑1 (MQ=255)

                                                              aGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            <  2:5095657/93‑1 (MQ=255)

                                                              aGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            >  2:3942463/1‑93 (MQ=255)

                                                               gTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           >  1:9446856/1‑93 (MQ=255)

                                                               gTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           >  1:4271756/1‑93 (MQ=255)

                                                               gTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           <  2:9704279/93‑1 (MQ=255)

                                                                tCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGc                          <  2:3244926/93‑1 (MQ=255)

                                                                tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGc                          >  1:451556/1‑93 (MQ=255)

                                                                tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGc                          >  2:2068737/1‑93 (MQ=255)

                                                                tCAATTTTCCCGCCAGTACGCTTCAGTCCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGc                          <  2:8549054/93‑1 (MQ=255)

                                                                tCAATTTGCCCGCCAGTACGCTTCAGTTCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGc                          <  1:6675958/93‑1 (MQ=255)

                                                                 taaTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         <  2:2438400/92‑1 (MQ=255)

                                                                 cAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCCGCGCGTCATCAACGTTTTTCTTCAGCg                         >  1:6103336/1‑93 (MQ=255)

                                                                 cAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         >  1:2469656/1‑93 (MQ=255)

                                                                 cAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         >  2:3085316/1‑93 (MQ=255)

                                                                 cAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         <  1:672065/93‑1 (MQ=255)

                                                                 cAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         <  2:4069682/93‑1 (MQ=255)

                                                                  aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        >  1:7988455/1‑93 (MQ=255)

                                                                   tttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       <  1:4079941/92‑1 (MQ=255)

                                                                   aTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       <  2:7278269/93‑1 (MQ=255)

                                                                   aTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       <  2:3315396/93‑1 (MQ=255)

                                                                    ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGaa                      <  1:6263745/93‑1 (MQ=255)

                                                                      ttCCCGCCAGTACGTTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATa                    <  1:2147732/93‑1 (MQ=255)

                                                                       ccccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTACCGCAGAAtgc                   <  2:8922679/92‑3 (MQ=255)

                                                                         ccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTg                 <  1:8842305/93‑1 (MQ=255)

                                                                         ccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTg                 <  1:2706261/93‑1 (MQ=255)

                                                                           gCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgcg               <  2:1526163/93‑1 (MQ=255)

                                                                            ccAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgcgc              >  1:8655618/1‑93 (MQ=255)

                                                                             cAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc             <  2:5989107/93‑1 (MQ=255)

                                                                             cAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc             >  1:3845783/1‑93 (MQ=255)

                                                                               gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg           >  1:2256541/1‑93 (MQ=255)

                                                                               gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg           >  1:5376226/1‑93 (MQ=255)

                                                                               gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg           <  1:4373363/93‑1 (MQ=255)

                                                                                tACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCgg          >  2:4912267/1‑93 (MQ=255)

                                                                                  cGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgca        <  1:7241961/93‑1 (MQ=255)

                                                                                  cGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgca        <  2:7078259/93‑1 (MQ=255)

                                                                                    cTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacg      >  1:8589464/1‑93 (MQ=255)

                                                                                      tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    <  1:3218563/93‑1 (MQ=255)

                                                                                      tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    <  1:7517480/93‑1 (MQ=255)

                                                                                      tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    <  1:5941549/93‑1 (MQ=255)

                                                                                        aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  >  2:3452525/1‑93 (MQ=255)

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTG‑CCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`