BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,652	A→G	Y513H (TAT→CAT)	yjcS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,652	0	A	G	99.7%	1042.0 / NA	325	Y513H (TAT→CAT)	yjcS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (136/188); minor base C (1/0); total (137/188)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

TGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG  >  NC_000913/4302562‑4302741

  gtgCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGccc                                                                                       <  2:7032505/93‑1 (MQ=255)

  gtgCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGccc                                                                                       <  2:5859156/93‑1 (MQ=255)

     ccGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGc                                                                                    >  2:9683768/1‑93 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 >  2:7880548/1‑93 (MQ=255)

           cTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCa                                                                              <  2:2948045/93‑1 (MQ=255)

            tGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaa                                                                             >  1:6200277/1‑93 (MQ=255)

              aaCTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAg                                                                           <  2:1478511/93‑1 (MQ=255)

                cTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAggt                                                                         >  2:6091912/1‑91 (MQ=255)

                   ttacACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAtt                                                                      <  1:6442433/90‑1 (MQ=255)

                   aTGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAtt                                                                      <  1:5292075/93‑1 (MQ=255)

                   aTGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAtt                                                                      <  2:6802264/93‑1 (MQ=255)

                   aTGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAtt                                                                      <  1:4519818/93‑1 (MQ=255)

                    tGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTc                                                                     <  1:8120233/93‑1 (MQ=255)

                    tGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTc                                                                     >  2:8879418/1‑93 (MQ=255)

                        ccccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCtt                                                                 <  2:2786201/93‑1 (MQ=255)

                        ccccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCtt                                                                 >  1:505798/1‑93 (MQ=255)

                         cccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTg                                                                >  2:3606526/1‑93 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               <  1:9678502/93‑1 (MQ=255)

                            ttCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAg                                                             >  2:6293950/1‑93 (MQ=255)

                            ttCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAg                                                             <  2:7163171/93‑1 (MQ=255)

                             tCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAGCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGa                                                            <  1:2775389/93‑1 (MQ=255)

                             tCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGa                                                            <  1:5691902/93‑1 (MQ=255)

                              cgcgCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAt                                                           <  2:3505241/93‑1 (MQ=255)

                               gcgcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAtt                                                          <  1:2727635/93‑1 (MQ=255)

                                cgcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttc                                                         <  2:7702550/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        <  2:2289600/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        <  1:3094825/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        >  1:955869/1‑93 (MQ=255)

                                  cAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGCTGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttctt                                                       >  2:2689095/1‑93 (MQ=255)

                                  cAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttctt                                                       <  1:9012455/93‑1 (MQ=255)

                                  cAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttctt                                                       <  2:1168062/93‑1 (MQ=255)

                                  cAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttctt                                                       <  1:1741110/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAGCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  1:9365901/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  2:2146434/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  1:5822186/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  2:5453688/93‑1 (MQ=255)

                                    gCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcg                                                     >  1:3634727/1‑93 (MQ=255)

                                     ctctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgc                                                    >  1:3905590/1‑93 (MQ=255)

                                     ctctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgc                                                    <  2:7331469/93‑1 (MQ=255)

                                      tctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGGTTCTTcgcg                                                   >  1:8872109/1‑93 (MQ=255)

                                        tttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAc                                                 >  2:7986776/1‑93 (MQ=255)

                                        tttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAc                                                 >  2:4257546/1‑93 (MQ=255)

                                        tttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAc                                                 <  1:1655067/93‑1 (MQ=255)

                                        tttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAc                                                 >  1:1389731/1‑93 (MQ=255)

                                         ttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAcc                                                <  2:3813541/93‑1 (MQ=255)

                                            gcgcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTgg                                             >  2:9347479/1‑93 (MQ=255)

                                            gcgcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTgg                                             >  1:10018914/1‑93 (MQ=255)

                                              gcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCGAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTc                                           >  1:4950053/1‑93 (MQ=255)

                                                cGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAc                                         <  2:1797470/93‑1 (MQ=255)

                                                 ggTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAcc                                        >  1:948234/1‑93 (MQ=255)

                                                 ggTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAcc                                        <  1:3741493/93‑1 (MQ=255)

                                                  gttaGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAccc                                       >  1:2143341/4‑93 (MQ=255)

                                                  gTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAccc                                       <  1:7609824/93‑1 (MQ=255)

                                                  gTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAccc                                       >  1:7127259/1‑93 (MQ=255)

                                                   tCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCg                                      >  2:9809645/1‑93 (MQ=255)

                                                   tCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCg                                      <  1:1252748/93‑1 (MQ=255)

                                                   gcAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCg                                      <  1:6762976/92‑1 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  2:3302010/93‑1 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  1:2395326/93‑1 (MQ=255)

                                                       gTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGCTTCTTCGCGACCTGGTCACCCGGGtg                                  >  1:7766695/1‑92 (MQ=255)

                                                         aGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTgg                                <  2:1587523/93‑1 (MQ=255)

                                                         aGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTgg                                <  1:1896923/93‑1 (MQ=255)

                                                          gAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggc                               <  2:8410684/93‑1 (MQ=255)

                                                           aaaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcg                              >  1:351938/1‑93 (MQ=255)

                                                           aaaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcg                              <  1:5445956/93‑1 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGTCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcgg                             <  2:8096653/93‑1 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAACTGGTCAAAGTTATTCGCTTGCAGATTCTTTGCGACCTGGTTACCCGGGTTggcgg                             >  1:1267162/1‑93 (MQ=255)

                                                               cgcgCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCaa                          <  2:3898664/93‑1 (MQ=255)

                                                                gcgcCATGTGGCGGGCTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAAt                         <  2:5695461/93‑1 (MQ=255)

                                                                gcgcCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAAt                         >  1:77193/1‑93 (MQ=255)

                                                                 cgcCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATc                        <  2:6297493/93‑1 (MQ=255)

                                                                   ccATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCAc                      >  2:1275325/1‑93 (MQ=255)

                                                                    cATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCAcc                     <  1:3199018/93‑1 (MQ=255)

                                                                    cATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCAcc                     <  1:410416/93‑1 (MQ=255)

                                                                     aTGTGGCGGACTGGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    <  2:4884417/93‑1 (MQ=255)

                                                                     aTGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    <  2:1712827/93‑1 (MQ=255)

                                                                     aTGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    >  1:8687786/1‑93 (MQ=255)

                                                                     aTGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    <  2:2460271/93‑1 (MQ=255)

                                                                     aTGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    <  1:8280687/93‑1 (MQ=255)

                                                                      tgtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTg                   <  2:7626880/93‑1 (MQ=255)

                                                                      tgtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTg                   >  1:4811124/1‑93 (MQ=255)

                                                                       gtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGt                  <  2:8231579/93‑1 (MQ=255)

                                                                       gtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGt                  <  2:4526412/93‑1 (MQ=255)

                                                                         ggCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGttt                >  1:8196645/1‑93 (MQ=255)

                                                                             gACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagc            >  1:9906626/1‑93 (MQ=255)

                                                                              aCTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagca           >  2:9368845/1‑93 (MQ=255)

                                                                              aCTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagca           >  1:7878094/1‑93 (MQ=255)

                                                                                 cGGACTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGtt        <  1:8370026/93‑1 (MQ=255)

                                                                                  gggcTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTc       >  2:5242843/4‑93 (MQ=255)

                                                                                  ggCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTc       >  2:669008/1‑93 (MQ=255)

                                                                                  ggCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTc       <  1:1154731/93‑1 (MQ=255)

                                                                                   gCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCt      <  2:6366608/93‑1 (MQ=255)

                                                                                    ccTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTg     <  1:62377/93‑1 (MQ=255)

                                                                                    ccTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTg     >  1:6593763/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  1:1086634/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  2:4080010/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  1:7015349/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   <  2:4982520/93‑1 (MQ=255)

                                                                                       gATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCg  >  1:3327106/1‑93 (MQ=255)

                                                                                       gATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCg  >  2:1945822/1‑93 (MQ=255)

TGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG  >  NC_000913/4302562‑4302741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`