BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,215	A→G	R522R (CGT→CGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,215	0	A	G	93.6%	1004.5 / 44.9	361	R522R (CGT→CGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base A (15/8); new base G (149/189); total (164/197)
Fisher's exact test for biased strand distribution p-value = 5.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

Only 100 of 380 total aligned reads displayed.

CCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTC  >  NC_000913/64125‑64305

ccAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACcgcg                                                                                          <  2:7117119/93‑1 (MQ=255)

ccAGACAAACGTTGAGTCGGCATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACg                                                                                          >  2:7218153/1‑93 (MQ=255)

 cAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCgcgc                                                                                         >  1:2883890/1‑93 (MQ=255)

  aGACAACCGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCa                                                                                        <  1:7974954/93‑1 (MQ=255)

  aGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCa                                                                                        <  1:664881/93‑1 (MQ=255)

  aGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCa                                                                                        <  2:220164/93‑1 (MQ=255)

  aGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCa                                                                                        >  2:2628512/1‑93 (MQ=255)

  aGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCa                                                                                        >  1:3159244/1‑93 (MQ=255)

       aaCGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTg                                                                                   >  1:3426558/1‑93 (MQ=255)

         cGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGAt                                                                                 >  2:5362880/1‑93 (MQ=255)

         cGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGAt                                                                                 >  2:6011408/1‑93 (MQ=255)

          gTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATc                                                                                <  2:3903136/93‑1 (MQ=255)

          gTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATc                                                                                >  2:806567/1‑93 (MQ=255)

           ttGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcg                                                                               >  2:1458142/1‑93 (MQ=255)

           ttGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcg                                                                               <  1:5031614/93‑1 (MQ=255)

            tGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcga                                                                              <  1:9973142/93‑1 (MQ=255)

             gAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgac                                                                             <  1:2341382/93‑1 (MQ=255)

              aGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacg                                                                            >  1:6026272/1‑93 (MQ=255)

               gTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacga                                                                           <  2:6337411/93‑1 (MQ=255)

               gTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacga                                                                           <  1:7078267/93‑1 (MQ=255)

               gTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacga                                                                           >  1:5498574/1‑93 (MQ=255)

                tCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacgag                                                                          >  1:6323610/1‑92 (MQ=255)

                tCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGAt                                                                          >  2:8389867/1‑93 (MQ=255)

                  ggTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGc                                                                        >  2:5758632/1‑93 (MQ=255)

                  ggTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGc                                                                        >  1:5647642/1‑93 (MQ=255)

                   gTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGcc                                                                       >  2:5129459/1‑93 (MQ=255)

                    tATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCa                                                                      <  2:2005634/93‑1 (MQ=255)

                      tCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGc                                                                    <  1:4468372/93‑1 (MQ=255)

                      tCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGc                                                                    <  2:9204368/93‑1 (MQ=255)

                      tCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGc                                                                    >  1:9776144/1‑93 (MQ=255)

                       cGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGcc                                                                   >  2:9323435/1‑93 (MQ=255)

                          cGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCcgcg                                                                <  1:9078582/93‑1 (MQ=255)

                          cGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCcgcg                                                                >  1:3246001/1‑93 (MQ=255)

                           gTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCgg                                                               >  2:3037789/1‑93 (MQ=255)

                            tAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGa                                                              <  1:6375602/93‑1 (MQ=255)

                            tAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGa                                                              >  2:1707971/1‑93 (MQ=255)

                            tAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGa                                                              >  1:858734/1‑93 (MQ=255)

                              gATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATc                                                            >  1:9473198/1‑93 (MQ=255)

                               aTAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCg                                                           >  1:7271392/1‑93 (MQ=255)

                                tAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCga                                                          <  1:3683697/93‑1 (MQ=255)

                                tAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCga                                                          >  1:1567663/1‑93 (MQ=255)

                                  aCGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaag                                                        <  2:9045380/93‑1 (MQ=255)

                                  aCGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaag                                                        >  2:7358185/1‑93 (MQ=255)

                                   cgtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaaga                                                       <  1:6941991/93‑1 (MQ=255)

                                   cgtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaaga                                                       <  2:42144/93‑1 (MQ=255)

                                      cgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGc                                                    >  1:1307706/1‑93 (MQ=255)

                                       gtAGCCCTGTTCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCg                                                   <  2:1889700/93‑1 (MQ=255)

                                       gtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCACTCGCGGATCGAAGAAGCg                                                   >  1:2726843/1‑93 (MQ=255)

                                          gCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCa                                                <  2:6553372/93‑1 (MQ=255)

                                          gCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCa                                                >  2:8572174/1‑93 (MQ=255)

                                          gCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCa                                                <  2:7880316/93‑1 (MQ=255)

                                           cccTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAg                                               <  2:9473619/93‑1 (MQ=255)

                                              tgtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCg                                            >  2:1114383/1‑93 (MQ=255)

                                              tgtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCg                                            >  2:3753610/1‑93 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                           >  1:2658081/1‑93 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGACCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                           <  2:278555/93‑1 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCAGGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                           <  2:2388889/93‑1 (MQ=255)

                                                tgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTTATGACCGCGCATGGTGATCGAAGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggt                                          <  2:3839436/93‑1 (MQ=255)

                                                 gCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtg                                         >  1:675819/1‑93 (MQ=255)

                                                  cTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtgg                                        <  2:5015912/93‑1 (MQ=255)

                                                   ttCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtggg                                       >  2:6783415/1‑92 (MQ=255)

                                                   ttCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtggg                                       >  1:5780626/1‑92 (MQ=255)

                                                   ttCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtggg                                       >  2:4084896/1‑92 (MQ=255)

                                                    tcaatcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtgggg                                      >  2:8901288/1‑91 (MQ=255)

                                                       atcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGGCg                                   >  2:9804419/1‑93 (MQ=255)

                                                       atcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCg                                   >  1:8108260/1‑93 (MQ=255)

                                                        tcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGa                                  >  2:9619691/1‑93 (MQ=255)

                                                        tcaaCGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGa                                  >  1:641324/1‑93 (MQ=255)

                                                          aaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGc                                <  1:7820143/93‑1 (MQ=255)

                                                           aCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCa                               <  2:6197049/93‑1 (MQ=255)

                                                            cGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCAc                              <  1:5696275/93‑1 (MQ=255)

                                                             gCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACg                             >  1:6188553/1‑93 (MQ=255)

                                                              cTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGc                            >  1:4327771/1‑93 (MQ=255)

                                                              cTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGc                            <  1:2475249/93‑1 (MQ=255)

                                                               tttGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGcc                           <  1:4013267/93‑1 (MQ=255)

                                                                ttGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCa                          >  2:3638012/1‑93 (MQ=255)

                                                                   gTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATaa                       >  2:4803625/1‑93 (MQ=255)

                                                                    tttGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATaaa                      >  1:1171389/1‑93 (MQ=255)

                                                                      tGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAg                    <  2:7625491/93‑1 (MQ=255)

                                                                      tGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAg                    >  1:8472914/1‑93 (MQ=255)

                                                                         cacATGATCTGATGACCGCGCATGATGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCa                 <  2:5588742/91‑1 (MQ=255)

                                                                           cATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCAtt               >  2:4393707/1‑93 (MQ=255)

                                                                            aTGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTc              >  1:7586736/1‑93 (MQ=255)

                                                                             tGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCa             <  1:5203421/93‑1 (MQ=255)

                                                                             tGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCa             <  1:4602183/93‑1 (MQ=255)

                                                                             tGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCa             <  2:8119732/93‑1 (MQ=255)

                                                                              gATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCat            >  1:955343/1‑93 (MQ=255)

                                                                              gATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGAGCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCat            <  1:2139944/93‑1 (MQ=255)

                                                                               aTCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatg           <  1:7856947/93‑1 (MQ=255)

                                                                               aTCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCata           >  2:3107301/1‑92 (MQ=255)

                                                                                tCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatga          <  2:8749777/93‑1 (MQ=255)

                                                                                 cTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatgat         <  1:4112991/93‑1 (MQ=255)

                                                                                 cTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatgat         >  1:8701286/1‑93 (MQ=255)

                                                                                  tgatgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatgatg        <  1:3097374/93‑1 (MQ=255)

                                                                                  tgatgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatgatg        >  1:2965271/1‑93 (MQ=255)

                                                                                   gatgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCAtgatga       <  2:7784582/93‑1 (MQ=255)

                                                                                    atgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATgatgat      <  2:5232698/93‑1 (MQ=255)

                                                                                    atgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATgatgat      >  1:10034691/1‑93 (MQ=255)

                                                                                     tgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGAtt     >  1:1752871/1‑93 (MQ=255)

                                                                                       aCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGAtttt   <  2:6909912/93‑1 (MQ=255)

CCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTC  >  NC_000913/64125‑64305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`