BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,328,807	A→G	Q243R (CAG→CGG)	proP →	proline permease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,328,807	0	A	G	100.0%	1201.2 / NA	371	Q243R (CAG→CGG)	proP	proline permease II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (195/176); total (195/176)

Only 100 of 383 total aligned reads displayed.

TCGGGCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCAGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGCCACCAACG  >  NC_000913/4328717‑4328896

tCGGGCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCggg                                                                                         >  1:9518206/1‑93 (MQ=255)

  gggCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATCAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGAt                                                                                       >  2:9530386/1‑93 (MQ=255)

   ggCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATg                                                                                      >  1:521701/1‑93 (MQ=255)

   ggCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATg                                                                                      >  1:5012002/1‑93 (MQ=255)

   ggCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATg                                                                                      >  2:8091427/1‑93 (MQ=255)

    gCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGATCAGGGCGACCGTGAAGGATTGCGGGATgg                                                                                     >  1:3920847/1‑93 (MQ=255)

    gCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATgg                                                                                     <  1:6613800/93‑1 (MQ=255)

    gCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATgg                                                                                     <  1:9137626/93‑1 (MQ=255)

    gCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATgg                                                                                     <  1:5956030/93‑1 (MQ=255)

      tttACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGcc                                                                                   <  2:7265293/93‑1 (MQ=255)

       ttACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGccc                                                                                  <  2:7754048/93‑1 (MQ=255)

       ttACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGccc                                                                                  <  1:8769734/93‑1 (MQ=255)

        tACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCg                                                                                 >  1:1925075/1‑93 (MQ=255)

          ccTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGaa                                                                               >  1:4810122/1‑93 (MQ=255)

           cTGCGCCATGCCTTCGAAGAGCCTCCGGCGTACCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGaaa                                                                              <  2:6790664/93‑1 (MQ=255)

              cgcCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGtc                                                                           <  2:3937299/93‑1 (MQ=255)

                ccATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGtctc                                                                         >  1:3069118/1‑93 (MQ=255)

                ccATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGtctc                                                                         <  1:6980605/93‑1 (MQ=255)

                ccATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGtctc                                                                         <  2:331746/93‑1 (MQ=255)

                ccATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGtctc                                                                         >  2:6053043/1‑93 (MQ=255)

                   tGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGtt                                                                      >  1:6793229/1‑93 (MQ=255)

                   tGCGCTGGAAGAGACTCCGGCGTTCCAGAAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGtt                                                                      <  1:3313380/93‑1 (MQ=255)

                    gcgcTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGttt                                                                     >  1:9165875/1‑93 (MQ=255)

                     cgcTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTa                                                                    <  2:3706340/93‑1 (MQ=255)

                       cTGGAAGAGCCTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTaaa                                                                  <  1:2717342/93‑1 (MQ=255)

                       cTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTaaa                                                                  <  2:1865585/93‑1 (MQ=255)

                       cTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTaaa                                                                  <  2:9635871/93‑1 (MQ=255)

                        tGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAag                                                                 <  2:7960802/93‑1 (MQ=255)

                           aaGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAgaga                                                              <  2:9855581/93‑1 (MQ=255)

                            agagACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGAt                                                             <  2:6885823/93‑1 (MQ=255)

                              agaCTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTg                                                           >  1:5697084/1‑93 (MQ=255)

                                 cTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCa                                                        <  1:9333807/93‑1 (MQ=255)

                                 cTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCa                                                        <  2:7476083/93‑1 (MQ=255)

                                  tCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCAc                                                       <  1:1583400/93‑1 (MQ=255)

                                   ccGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACt                                                      >  1:2942006/1‑93 (MQ=255)

                                   ccGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGCCTCGTTTAAAGAGATTGCCACt                                                      >  2:1487920/1‑93 (MQ=255)

                                    cGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTa                                                     >  2:7195130/1‑93 (MQ=255)

                                      gCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTaaa                                                   <  1:3624371/93‑1 (MQ=255)

                                      gCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTaaa                                                   >  1:7059514/1‑93 (MQ=255)

                                      gCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTaaa                                                   <  1:1821868/93‑1 (MQ=255)

                                       cGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAAt                                                  <  2:1683776/93‑1 (MQ=255)

                                        gtgccAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATa                                                 <  1:6905812/90‑1 (MQ=255)

                                        gTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATa                                                 <  2:4241750/93‑1 (MQ=255)

                                         ttCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATAc                                                >  2:896438/1‑93 (MQ=255)

                                          tCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACt                                               <  2:4059182/93‑1 (MQ=255)

                                           ccAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTg                                              <  1:6162334/93‑1 (MQ=255)

                                            cagcagCATGTCGATAAACTGGAACAGAGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTgg                                             <  1:5443612/93‑1 (MQ=255)

                                              gcagcaTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGgcg                                           <  2:9812467/93‑1 (MQ=255)

                                              gcagcaTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGgcg                                           >  2:2206376/1‑93 (MQ=255)

                                               cagcaTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGgcgc                                          <  1:7885752/93‑1 (MQ=255)

                                                 gcaTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAg                                        >  1:7148272/1‑93 (MQ=255)

                                                  caTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGc                                       >  2:9217963/1‑93 (MQ=255)

                                                   aTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGcc                                      >  2:5201624/1‑93 (MQ=255)

                                                   aTGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGcc                                      <  1:600722/93‑1 (MQ=255)

                                                    tGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCt                                     >  2:6554372/1‑93 (MQ=255)

                                                    tGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCt                                     >  1:1193350/1‑93 (MQ=255)

                                                    tGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCt                                     <  2:2972907/93‑1 (MQ=255)

                                                    tGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCt                                     >  1:1054279/1‑93 (MQ=255)

                                                    tGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCt                                     >  1:6699463/1‑93 (MQ=255)

                                                      tCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGt                                   <  2:1008991/93‑1 (MQ=255)

                                                      gggATAAACTGGAACAGGGCGGCCGTGTAGGTGTTCGGGAGGGCCCGAAAGTCTCGTTTAAAGACAGTGCCATTAAATACTGGCGCAGCCTGt                                   <  1:7541540/91‑1 (MQ=255)

                                                       cGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGtt                                  <  1:2558306/93‑1 (MQ=255)

                                                       cGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGtt                                  <  1:4431446/93‑1 (MQ=255)

                                                         aTAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGa                                <  2:3866917/93‑1 (MQ=255)

                                                         aTAAACTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGa                                >  2:8595831/1‑93 (MQ=255)

                                                           aaaCTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACa                              <  2:7858804/93‑1 (MQ=255)

                                                           aaaCTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACa                              <  1:2982624/93‑1 (MQ=255)

                                                           aaaCTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACa                              >  2:5358546/1‑93 (MQ=255)

                                                              cTGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGGTTGCCACTAAATACTGGCGCAGCCTGTTGACgtgt                           >  1:1144868/1‑93 (MQ=255)

                                                               tGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTa                          >  1:3397463/1‑93 (MQ=255)

                                                               tGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTa                          >  2:8280125/1‑93 (MQ=255)

                                                               tGGAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTa                          <  2:6431386/93‑1 (MQ=255)

                                                                ggAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTAt                         >  2:580302/1‑93 (MQ=255)

                                                                gcaaCAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTAt                         <  1:2114193/91‑1 (MQ=255)

                                                                 gAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTAtt                        >  1:3547978/1‑93 (MQ=255)

                                                                 gAACAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTAtt                        <  1:1543664/93‑1 (MQ=255)

                                                                  aaCAGGGCGAGCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTg                       <  2:6650722/93‑1 (MQ=255)

                                                                  aaCAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGCAAGTCTCGTTTAAAGAGATTGCCACTACATACTGGCGCAGCCTGTTGACATGTATTg                       >  1:9849464/1‑93 (MQ=255)

                                                                  aaCAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTg                       <  1:8646364/93‑1 (MQ=255)

                                                                   aCAGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTgg                      >  2:4672218/1‑93 (MQ=255)

                                                                     aGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTc                    <  2:2439392/93‑1 (MQ=255)

                                                                     aGGGCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTc                    >  2:7070971/1‑93 (MQ=255)

                                                                      gggCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCt                   >  1:6285874/1‑93 (MQ=255)

                                                                      gggCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCt                   >  1:8128205/1‑93 (MQ=255)

                                                                      gggCGACCGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCt                   <  1:2299588/93‑1 (MQ=255)

                                                                            ccGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAAt             >  2:5664124/1‑93 (MQ=255)

                                                                             cGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAAtt            >  2:4408756/1‑93 (MQ=255)

                                                                             cGTGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAAtt            <  1:4727387/93‑1 (MQ=255)

                                                                               tGAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGc          >  1:959938/1‑93 (MQ=255)

                                                                                gAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGcc         <  1:835603/93‑1 (MQ=255)

                                                                                gAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGcc         <  2:6261114/93‑1 (MQ=255)

                                                                                gAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGcc         <  1:2817422/93‑1 (MQ=255)

                                                                                gAAGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGcc         >  2:4890310/1‑93 (MQ=255)

                                                                                 aaGGTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGcca        <  1:9777749/93‑1 (MQ=255)

                                                                                   ggTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGTCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGccacc      >  1:4143633/1‑93 (MQ=255)

                                                                                   ggTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGccacc      >  2:8410698/1‑93 (MQ=255)

                                                                                   ggTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGccacc      >  1:4398352/1‑93 (MQ=255)

                                                                                    gTTTGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTTACATGTATTGGTCTGGTAATTGccacca     >  2:4746578/1‑93 (MQ=255)

                                                                                      ttGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGCCACCAAc   <  2:1312962/93‑1 (MQ=255)

                                                                                       tGCGGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGCCACCAACg  <  1:7975843/93‑1 (MQ=255)

TCGGGCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAGCATGTCGATAAACTGGAACAGGGCGACCGTGAAGGTTTGCAGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACTAAATACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGCCACCAACG  >  NC_000913/4328717‑4328896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`