BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,832	T→C	M387V (ATG→GTG)	yjeH ←	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,832	0	T	C	99.2%	1224.1 / ‑2.1	385	M387V (ATG→GTG)	yjeH	putative transport
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (188/194); total (189/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

Only 100 of 401 total aligned reads displayed.

TTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAGCATGCATA  >  NC_000913/4366742‑4366921

 tttGCCATTTTCCGGCGTTTTTCGTTTTCGCAGCAAGAGCCGTAATCCCGCCAGCATGATCAGCGCATAGAGTCTTTTCCAGCCGACCACTGc                                                                                        >  2:869771/3‑93 (MQ=255)

 tATGCGATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGc                                                                                        <  2:1667150/93‑1 (MQ=255)

   tGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCCGCGCATAGAGCCTTTTCCAGCGGACCACTGccc                                                                                      >  1:3507733/1‑92 (MQ=255)

   tGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCa                                                                                      <  2:8396419/93‑1 (MQ=255)

   tGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCa                                                                                      <  2:6225919/93‑1 (MQ=255)

   tGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCa                                                                                      <  1:7293893/93‑1 (MQ=255)

    gCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAg                                                                                     <  2:3661534/93‑1 (MQ=255)

      cATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTa                                                                                   >  1:5502272/1‑93 (MQ=255)

       aTTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTaa                                                                                  <  1:8301364/93‑1 (MQ=255)

        ttttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAAc                                                                                 >  2:7825256/1‑93 (MQ=255)

        ttttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAAc                                                                                 >  2:4755063/1‑93 (MQ=255)

        ttttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAAc                                                                                 >  2:149181/1‑93 (MQ=255)

        ttttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAAc                                                                                 >  1:7586252/1‑93 (MQ=255)

        ttttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAAc                                                                                 >  1:7502915/1‑93 (MQ=255)

        ttttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAAc                                                                                 >  1:5179577/1‑93 (MQ=255)

          ttCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAg                                                                               <  1:1196590/93‑1 (MQ=255)

           tCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGa                                                                              >  1:9777753/1‑93 (MQ=255)

               gCGTTTTTCGTTTTGGAAACAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGc                                                                          <  1:10023166/93‑1 (MQ=255)

                cttttttCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCa                                                                         <  2:7477373/91‑1 (MQ=255)

                cGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCa                                                                         <  1:2058262/93‑1 (MQ=255)

                  tttttCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCTTGATCAGCGCTTAGAGACTTTTCCAGCGGCCCACTGCCAGTAACAGAACGCATa                                                                       >  2:2542170/1‑93 (MQ=255)

                   ttttCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATaa                                                                      <  2:7055008/93‑1 (MQ=255)

                   tgttCGTTTTGGCAGCAACAGCCATACCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATaa                                                                      <  1:291962/91‑1 (MQ=255)

                    tttCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAAc                                                                     >  1:4617999/1‑93 (MQ=255)

                     ttCGTTTTGGCAGCACCAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACa                                                                    <  2:5034579/93‑1 (MQ=255)

                       cGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGc                                                                  <  2:9142828/93‑1 (MQ=255)

                        gTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGcc                                                                 >  2:203359/1‑93 (MQ=255)

                        gTTTGGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACGGAACGCATAACAGcc                                                                 <  1:4955669/93‑1 (MQ=255)

                          tttGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCg                                                               >  2:4573836/1‑93 (MQ=255)

                              gcagcaACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCaa                                                           >  1:6437881/1‑93 (MQ=255)

                                agcaACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAacc                                                         <  1:609626/93‑1 (MQ=255)

                                 gcaACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAacca                                                        <  1:1493144/93‑1 (MQ=255)

                                  gaccAGCCATACCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAaccac                                                       <  2:8257321/90‑1 (MQ=255)

                                  caACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAaccac                                                       <  1:1711730/93‑1 (MQ=255)

                                   aaCAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAaccacc                                                      >  1:8048828/1‑93 (MQ=255)

                                    aCAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCg                                                     >  1:6878132/1‑93 (MQ=255)

                                    aCAGCAATACCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCg                                                     <  2:9702722/93‑1 (MQ=255)

                                     cAGCCATACCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCCGTAACAGAACGCATAACAGCCCGCCAACCACCGc                                                    <  2:6371118/93‑1 (MQ=255)

                                       gCCATAGCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCa                                                  >  2:7209481/1‑93 (MQ=255)

                                       gCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCa                                                  <  1:3112999/93‑1 (MQ=255)

                                       gCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCa                                                  >  2:3062448/1‑93 (MQ=255)

                                       gCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCa                                                  <  2:8504305/93‑1 (MQ=255)

                                        ccATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCag                                                 >  2:3909776/1‑93 (MQ=255)

                                          aTACCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagta                                               <  2:3359493/93‑1 (MQ=255)

                                          aTAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagta                                               >  1:4364793/1‑93 (MQ=255)

                                          aTAACCCCGCCAGCATGATCAGCGCATAGAGACTTTCCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagta                                               <  1:7347987/93‑1 (MQ=255)

                                           tAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagtag                                              >  2:3842742/1‑93 (MQ=255)

                                           tAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagtag                                              >  1:2427899/1‑93 (MQ=255)

                                           tAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagtag                                              >  1:1358833/1‑93 (MQ=255)

                                           tAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagtag                                              >  1:7048164/1‑93 (MQ=255)

                                           tAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagtag                                              >  1:2676226/1‑93 (MQ=255)

                                            acccccGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCagtagt                                             <  2:9414339/91‑1 (MQ=255)

                                             aCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTc                                            <  1:8594983/93‑1 (MQ=255)

                                              ccccGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCg                                           <  1:9954497/93‑1 (MQ=255)

                                               ctcGCCAGCATGATCAGCTCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGa                                          <  1:9483847/91‑1 (MQ=255)

                                               cccGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGa                                          <  1:4725006/93‑1 (MQ=255)

                                                ccGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGAt                                         >  2:7714013/1‑93 (MQ=255)

                                                 cGCCAGCATGATCAGCGCAGAGAGACTTTTCCAGACGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATa                                        <  2:6147296/93‑1 (MQ=255)

                                                  gcgaGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATaa                                       <  1:612691/90‑1 (MQ=255)

                                                  gCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATaa                                       <  1:5081730/93‑1 (MQ=255)

                                                   ccAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAAc                                      >  2:5747544/1‑93 (MQ=255)

                                                   ccAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAAc                                      >  1:3896023/1‑93 (MQ=255)

                                                    cAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACg                                     <  1:8840896/93‑1 (MQ=255)

                                                     aGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACgc                                    >  2:3415052/1‑92 (MQ=255)

                                                     aGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGt                                    <  2:2815597/93‑1 (MQ=255)

                                                       cATGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTcc                                  >  2:8069282/1‑93 (MQ=255)

                                                        aTGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCt                                 <  2:4347801/93‑1 (MQ=255)

                                                        aTGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCt                                 <  2:5858415/93‑1 (MQ=255)

                                                         tGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCtt                                >  2:3512367/1‑93 (MQ=255)

                                                         tGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCtt                                >  2:9579646/1‑93 (MQ=255)

                                                         tGATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCACGCCAACCACCGCCAGTAGTCGATAACGTCCtt                                >  1:1791029/1‑93 (MQ=255)

                                                         tGATCAGCGCATAGAGACTTTTCAAGCCGACCACTGCCAGCAACAGAACGCATAACAGCCCCCCAACCACCGCCAGTAGTCGATAACGTCCtt                                >  1:6541194/1‑93 (MQ=255)

                                                          gATCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTg                               >  2:3266889/1‑93 (MQ=255)

                                                           aTCAGCGCATAGAGCCTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTGCTTGc                              <  2:5983160/93‑1 (MQ=255)

                                                            tCAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCa                             >  2:3560542/1‑93 (MQ=255)

                                                             cAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCaa                            <  1:3404224/93‑1 (MQ=255)

                                                             cAGCGCATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCaa                            <  1:6647225/93‑1 (MQ=255)

                                                                ggcATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCaataa                         <  1:3654030/92‑1 (MQ=255)

                                                                cgcATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCaataa                         <  1:5944793/93‑1 (MQ=255)

                                                                 gcATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCaataat                        <  1:9273291/93‑1 (MQ=255)

                                                                  cATAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAAtt                       >  1:3179777/1‑93 (MQ=255)

                                                                    tAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTa                     <  2:175741/93‑1 (MQ=255)

                                                                    tAGAGACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTa                     <  2:1598429/93‑1 (MQ=255)

                                                                     agagACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTAc                    >  2:9748675/1‑93 (MQ=255)

                                                                     agagACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTAc                    <  2:8811338/93‑1 (MQ=255)

                                                                     agagACTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTAc                    <  1:1537346/93‑1 (MQ=255)

                                                                      aataCTTTTCCCGCCGACCACTGCCAGTAACAGAACGCATAACATCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACa                   <  2:2244041/90‑1 (MQ=255)

                                                                       agaCTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAg                  >  2:1226428/1‑93 (MQ=255)

                                                                        gaCTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGc                 <  1:7818908/93‑1 (MQ=255)

                                                                        gaCTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGc                 >  1:5389127/1‑93 (MQ=255)

                                                                         aCTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGcc                >  2:1868810/1‑93 (MQ=255)

                                                                          cTTTTCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCt               >  2:532907/1‑93 (MQ=255)

                                                                            tttCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGc             <  2:4158044/93‑1 (MQ=255)

                                                                              tCCAGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCa           <  2:7645082/93‑1 (MQ=255)

                                                                                 aGCCGACCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAgca        <  2:6802678/93‑1 (MQ=255)

                                                                                     gACCACTGCCAGTAACAGACCGCATAACCGCCCGCCAACCACCGCCAGTAGTCGATAACGACCTTGCAATAATTTACAGCCTGCCAgcatgca    <  1:8977247/93‑1 (MQ=255)

                                                                                      aCCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAgcatgcat   >  1:9148909/1‑93 (MQ=255)

                                                                                      aCCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAgcatgcat   >  1:5761895/1‑93 (MQ=255)

                                                                                      aCCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAgcatgcat   >  2:6671841/1‑93 (MQ=255)

                                                                                      aCCACTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAgcatgcaa   >  2:8210768/1‑92 (MQ=255)

TTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAGCATGCATA  >  NC_000913/4366742‑4366921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`