BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,292	T→A	S101R (AGT→AGA)	mutL →	MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,292	0	T	A	99.4%	1278.5 / NA	358	S101R (AGT→AGA)	mutL	MutL
Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (176/179); minor base G (2/0); total (178/180)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 372 total aligned reads displayed.

CTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395203‑4395381

ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATc                                                                                        <  1:9121724/93‑1 (MQ=255)

ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATc                                                                                        >  2:7584085/1‑93 (MQ=255)

 tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCa                                                                                       <  2:1066256/93‑1 (MQ=255)

  ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAg                                                                                      <  2:4497378/93‑1 (MQ=255)

  ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAg                                                                                      >  2:540710/1‑93 (MQ=255)

   gcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGt                                                                                     >  1:5285500/1‑93 (MQ=255)

   gcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGt                                                                                     <  2:1754375/93‑1 (MQ=255)

    cTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGtt                                                                                    <  2:1637507/93‑1 (MQ=255)

    cTCGTCATGCCACCAGTAAAATCGCCGCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGtt                                                                                    <  2:5042299/93‑1 (MQ=255)

     tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   <  1:1946455/93‑1 (MQ=255)

     tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   >  2:3943762/1‑93 (MQ=255)

      cGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCg                                                                                  <  1:6711587/93‑1 (MQ=255)

         cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGtt                                                                               >  2:9860906/1‑93 (MQ=255)

         cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCGGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGtt                                                                               <  2:5852206/93‑1 (MQ=255)

          aTGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGttt                                                                              <  1:2630995/93‑1 (MQ=255)

          aTGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGttt                                                                              >  2:2482810/1‑93 (MQ=255)

          aTGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGttt                                                                              >  2:8007788/1‑93 (MQ=255)

             ccaccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTccc                                                                           <  2:612258/93‑1 (MQ=255)

              caccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCg                                                                          >  2:6894662/1‑93 (MQ=255)

                ccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGcc                                                                        >  1:7249987/1‑93 (MQ=255)

                  aGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTg                                                                      <  2:7303636/93‑1 (MQ=255)

                   gTAAAATCCCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGCGAATCAGTTCGGTTTCCCGCCTGa                                                                     <  2:2841906/93‑1 (MQ=255)

                    tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGGATCAGTTCGGTTTCCCGCCTGAc                                                                    >  2:5542661/1‑93 (MQ=255)

                    tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAc                                                                    <  2:2692469/93‑1 (MQ=255)

                    tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAc                                                                    >  2:1521401/1‑93 (MQ=255)

                     aaaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACg                                                                   <  1:8316247/93‑1 (MQ=255)

                        aTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTc                                                                <  1:3709142/93‑1 (MQ=255)

                         tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCa                                                               >  1:1140891/1‑93 (MQ=255)

                           gCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACt                                                             >  1:2095203/1‑93 (MQ=255)

                             ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           <  1:3948984/93‑1 (MQ=255)

                              tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          >  1:3031083/1‑93 (MQ=255)

                              tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          >  2:9498210/1‑93 (MQ=255)

                              tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          <  1:7642348/93‑1 (MQ=255)

                              tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          >  1:8859484/1‑93 (MQ=255)

                                tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGATTCCCGCCTGACGCTCACTTCACg                                                        >  1:4965951/1‑93 (MQ=255)

                                  tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCa                                                      >  2:4714866/1‑93 (MQ=255)

                                  tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCa                                                      >  2:6063311/1‑93 (MQ=255)

                                  tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCa                                                      <  1:9044901/93‑1 (MQ=255)

                                   ggACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAc                                                     >  2:6441504/1‑93 (MQ=255)

                                    gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAcc                                                    <  1:2407510/93‑1 (MQ=255)

                                      cGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGc                                                  <  2:6826885/93‑1 (MQ=255)

                                        aTCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGCGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAg                                                >  1:2872767/1‑93 (MQ=255)

                                        aTCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCCCAg                                                >  1:8478369/1‑93 (MQ=255)

                                         tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  1:6513373/1‑93 (MQ=255)

                                         tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  2:8516413/1‑93 (MQ=255)

                                            cGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAca                                            >  1:1869006/1‑93 (MQ=255)

                                              aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          <  1:8202077/93‑1 (MQ=255)

                                              aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          >  1:7902025/1‑93 (MQ=255)

                                              aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          >  2:1887633/1‑93 (MQ=255)

                                              aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          >  1:1453836/1‑93 (MQ=255)

                                               aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         <  2:4180075/93‑1 (MQ=255)

                                               aGCCATAATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCTCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         <  2:6754449/93‑1 (MQ=255)

                                                  cATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGa                                      <  1:2723560/93‑1 (MQ=255)

                                                  cATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGa                                      <  1:5455103/93‑1 (MQ=255)

                                                     tATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGc                                   <  2:8660636/93‑1 (MQ=255)

                                                     tATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTACCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGc                                   >  2:9149438/1‑93 (MQ=255)

                                                       tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 <  2:9347347/93‑1 (MQ=255)

                                                       tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 >  1:343106/1‑93 (MQ=255)

                                                       tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 <  2:2927864/93‑1 (MQ=255)

                                                        cAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTg                                >  1:9863543/1‑93 (MQ=255)

                                                        cAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTg                                <  1:2293090/93‑1 (MQ=255)

                                                        cAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTg                                <  1:1347368/93‑1 (MQ=255)

                                                          gCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGc                              <  1:5030852/93‑1 (MQ=255)

                                                           ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             >  2:3666362/1‑93 (MQ=255)

                                                           ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  1:7341378/93‑1 (MQ=255)

                                                            cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            <  1:2356470/93‑1 (MQ=255)

                                                            cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            <  2:8211275/93‑1 (MQ=255)

                                                            cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            <  2:8789390/93‑1 (MQ=255)

                                                             tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  1:4350567/1‑93 (MQ=255)

                                                              gggCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGc                          >  1:10047478/1‑93 (MQ=255)

                                                              gggCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGc                          >  1:8674780/1‑93 (MQ=255)

                                                               ggCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGcc                         >  2:2316535/1‑93 (MQ=255)

                                                                 cTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa                       <  2:2982909/93‑1 (MQ=255)

                                                                 cTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa                       <  2:101146/93‑1 (MQ=255)

                                                                  tttCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTAt                      >  1:5853492/1‑93 (MQ=255)

                                                                   ttCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATg                     <  2:4669246/93‑1 (MQ=255)

                                                                    tCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc                    >  1:7206216/1‑93 (MQ=255)

                                                                       cgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGa                 <  2:3208962/93‑1 (MQ=255)

                                                                       cgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGa                 >  1:6296289/1‑93 (MQ=255)

                                                                       cgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGa                 >  1:8996569/1‑93 (MQ=255)

                                                                       cgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGACTATGCCGa                 >  2:6344485/1‑93 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  2:3378342/93‑1 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  1:1560316/93‑1 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  1:1656517/93‑1 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                >  2:2249969/1‑93 (MQ=255)

                                                                         gTGAGGCGCTGGCGAGAGTCAGTTCGGTTTCCCGCCTGACGCTAACTGCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               <  2:9630718/93‑1 (MQ=255)

                                                                         gTGAGGCGCTGCCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               <  2:5910378/93‑1 (MQ=255)

                                                                          tGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAgg              >  1:4830760/1‑93 (MQ=255)

                                                                           gAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAggg             >  2:2057745/1‑93 (MQ=255)

                                                                            aGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgc            >  1:4559425/1‑93 (MQ=255)

                                                                            aGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgc            <  2:3516246/93‑1 (MQ=255)

                                                                             ggCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcg           >  2:311757/1‑93 (MQ=255)

                                                                             ggCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGACTATGCCGAAGGgcg           >  2:7011950/1‑93 (MQ=255)

                                                                                 cTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGat       <  1:3218338/93‑1 (MQ=255)

                                                                                  tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      <  2:4358369/93‑1 (MQ=255)

                                                                                  tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      <  1:9339908/93‑1 (MQ=255)

                                                                                  tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGCGCGCGata      >  1:6061627/1‑93 (MQ=255)

                                                                                   ggCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat     >  1:9569882/1‑93 (MQ=255)

                                                                                     cGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGa   <  1:704391/93‑1 (MQ=255)

                                                                                      gagaATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGaa  >  1:5226704/1‑93 (MQ=255)

CTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395203‑4395381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`