BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,925	A→G	I79V (ATC→GTC)	b4212 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,925	0	A	G	98.7%	1188.8 / ‑2.1	381	I79V (ATC→GTC)	b4212	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (163/213); total (165/216)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

AGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGAC  >  NC_000913/4431835‑4432014

aGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTc                                                                                         <  2:8133148/93‑1 (MQ=255)

aGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTc                                                                                         <  2:737605/93‑1 (MQ=255)

  cGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTcgc                                                                                       <  1:1751581/93‑1 (MQ=255)

     ccTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTa                                                                                    <  2:8371635/93‑1 (MQ=255)

       tGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATc                                                                                  <  1:674823/93‑1 (MQ=255)

       tGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATc                                                                                  <  2:2938508/93‑1 (MQ=255)

       tGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTCTTCTTAACCGTGTCGCGTATc                                                                                  <  2:4277672/93‑1 (MQ=255)

          ggcgCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCgg                                                                               <  2:5195867/93‑1 (MQ=255)

           gcgcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggt                                                                              <  2:6900246/93‑1 (MQ=255)

           gcgcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggt                                                                              <  2:3661891/93‑1 (MQ=255)

           gcgcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggt                                                                              >  1:423204/1‑93 (MQ=255)

            cgcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtg                                                                             <  2:6133195/93‑1 (MQ=255)

             gcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtgg                                                                            <  2:2699590/93‑1 (MQ=255)

             gcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtgg                                                                            <  2:4607304/93‑1 (MQ=255)

             gcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtgg                                                                            <  2:9053826/93‑1 (MQ=255)

             gcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGATAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtgg                                                                            >  1:627215/1‑93 (MQ=255)

              cAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtggt                                                                           <  2:7305999/93‑1 (MQ=255)

              cAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCggtggt                                                                           <  1:259743/93‑1 (MQ=255)

               aaaaTCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGGTTTCTTAACCGTGTCGCGTATCCGgtggtg                                                                          >  2:2039616/1‑93 (MQ=255)

                aaaTCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTgc                                                                         <  1:4550117/93‑1 (MQ=255)

                 aaTCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTgcc                                                                        >  1:7367546/1‑93 (MQ=255)

                  aTCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTgccg                                                                       <  2:3477689/93‑1 (MQ=255)

                        ggggTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCAt                                                                 >  1:4723428/1‑93 (MQ=255)

                        ggggTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCAt                                                                 >  2:6351307/1‑93 (MQ=255)

                         gggTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCAtg                                                                <  1:307023/93‑1 (MQ=255)

                          ggTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCAtgt                                                               <  2:4416271/93‑1 (MQ=255)

                           gtgagtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCAtgtg                                                              <  1:3312260/93‑1 (MQ=255)

                            tgagtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTgg                                                             <  2:2467520/93‑1 (MQ=255)

                             gagtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGa                                                            >  2:2142924/1‑93 (MQ=255)

                              agtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGaa                                                           <  2:927157/93‑1 (MQ=255)

                              agtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCATATCCGGTGGTGCCGCCGCATGTGGaa                                                           >  1:4070193/1‑93 (MQ=255)

                               gtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAat                                                          <  1:4105752/93‑1 (MQ=255)

                               gtgaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAat                                                          >  1:9672979/1‑93 (MQ=255)

                                 gaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAatat                                                        >  1:8459916/1‑93 (MQ=255)

                                 gaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAatat                                                        >  2:7758380/1‑93 (MQ=255)

                                 gaAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAatat                                                        >  2:3535147/1‑93 (MQ=255)

                                  aaaaGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAAtata                                                       <  1:6132922/93‑1 (MQ=255)

                                   aaaGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAg                                                      >  2:9756454/1‑93 (MQ=255)

                                    aaGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGc                                                     >  1:8076833/1‑93 (MQ=255)

                                     aGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGcc                                                    <  2:3122203/93‑1 (MQ=255)

                                      gATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCt                                                   <  1:5837827/93‑1 (MQ=255)

                                         gCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAc                                                <  2:1762853/93‑1 (MQ=255)

                                         gCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAc                                                <  2:5591541/93‑1 (MQ=255)

                                           ttGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAcgc                                              <  1:4002374/93‑1 (MQ=255)

                                            tGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAcgcc                                             >  2:3612864/1‑93 (MQ=255)

                                              cgcAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAcgccgc                                           <  1:7174211/93‑1 (MQ=255)

                                              cgcAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAcgccgc                                           <  1:875168/93‑1 (MQ=255)

                                              cgcAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCAcgccgc                                           <  2:1052958/93‑1 (MQ=255)

                                                cAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTg                                         <  1:893630/93‑1 (MQ=255)

                                                 aGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTgg                                        <  1:4440348/93‑1 (MQ=255)

                                                 aGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTgg                                        >  2:2758231/1‑93 (MQ=255)

                                                  gTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTggg                                       >  1:918737/1‑93 (MQ=255)

                                                  gTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTggg                                       >  1:5091893/1‑93 (MQ=255)

                                                   tCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGc                                      >  1:2172197/1‑93 (MQ=255)

                                                   tCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGc                                      <  1:4809857/93‑1 (MQ=255)

                                                     gTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGa                                    <  1:8313492/93‑1 (MQ=255)

                                                        aCAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGcg                                 >  1:2090418/1‑92 (MQ=255)

                                                        aCAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCa                                 >  2:6519972/1‑93 (MQ=255)

                                                        aCAGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCa                                 <  2:5907444/93‑1 (MQ=255)

                                                          aGGCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAgg                               >  2:1430836/1‑93 (MQ=255)

                                                           ggCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAggg                              >  1:4961607/1‑92 (MQ=255)

                                                           ggCGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGt                              <  1:2383932/93‑1 (MQ=255)

                                                             cGTTAGAACAGGATGGTTTTCTTAGCCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGa                            <  2:9649382/93‑1 (MQ=255)

                                                             cGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGa                            <  2:3766867/93‑1 (MQ=255)

                                                             cGTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGa                            <  2:8593716/93‑1 (MQ=255)

                                                             cGTGAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGa                            <  1:2222995/93‑1 (MQ=255)

                                                             agTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGa                            <  1:3605322/92‑1 (MQ=255)

                                                              gTTAGATCAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGGCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAg                           >  2:5773653/1‑93 (MQ=255)

                                                              gTTAGAACAGGATGGTTTTCTTAACGCTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAg                           <  1:9366114/93‑1 (MQ=255)

                                                              gTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAg                           >  2:7140759/1‑93 (MQ=255)

                                                              gTTAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAg                           >  1:7053410/1‑93 (MQ=255)

                                                               ttAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGc                          >  1:3368403/1‑93 (MQ=255)

                                                               ttAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGGGAgg                          >  2:1987695/1‑92 (MQ=255)

                                                               ttAGAACAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGGGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGc                          >  2:1560479/1‑93 (MQ=255)

                                                                   aaCAGGATGGTTTTCTTAAGCTGGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTTGGGCCGCTGGGCGAGCTGGTGAGCGaaa                      <  2:2642441/93‑1 (MQ=255)

                                                                   aaCAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGaaa                      <  1:4176630/93‑1 (MQ=255)

                                                                    aCAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGaaag                     >  2:3363316/1‑92 (MQ=255)

                                                                    aCAGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGaaaa                     >  2:3188132/1‑93 (MQ=255)

                                                                      aGGATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAgg                   >  1:5646927/1‑93 (MQ=255)

                                                                        gATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGtt                 >  1:726356/1‑93 (MQ=255)

                                                                        gATGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGtt                 >  2:4124480/1‑93 (MQ=255)

                                                                          tGGTTTTCTTACCCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTtgc               <  1:2996346/93‑1 (MQ=255)

                                                                          tGGTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTtgc               <  1:5147823/93‑1 (MQ=255)

                                                                          tGGTTTTCTTAACCGGGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTtgc               <  2:5000088/93‑1 (MQ=255)

                                                                           ggTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTtgct              >  2:5431584/1‑93 (MQ=255)

                                                                           ggTTTTCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTtgct              <  1:9860861/93‑1 (MQ=255)

                                                                             ttttCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTtgctgc            <  1:5995063/93‑1 (MQ=255)

                                                                              tttCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCa           <  1:1304740/93‑1 (MQ=255)

                                                                              tttCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCa           <  1:7624711/93‑1 (MQ=255)

                                                                              tttCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCa           <  1:10004468/93‑1 (MQ=255)

                                                                               ttCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCAc          <  1:547858/93‑1 (MQ=255)

                                                                                tCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACt         >  2:4800213/1‑93 (MQ=255)

                                                                                tCTTAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACt         <  1:2795927/93‑1 (MQ=255)

                                                                                  ttAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTgg       >  2:229748/1‑93 (MQ=255)

                                                                                  ttAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTgg       >  1:7956218/1‑93 (MQ=255)

                                                                                   tAACCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGc      <  2:2835253/93‑1 (MQ=255)

                                                                                     aCCGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCgg    >  2:8729352/1‑93 (MQ=255)

                                                                                      ccGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGa   <  2:8871133/93‑1 (MQ=255)

                                                                                       cGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGAc  <  2:1330582/93‑1 (MQ=255)

                                                                                       cGTGTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGAc  >  1:5221522/1‑93 (MQ=255)

AGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGAC  >  NC_000913/4431835‑4432014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`