Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,441,383 T→C S475S (AGT→AGC)  ytfM → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,441,3830TC99.7% 1171.4 / ‑5.2 362S475S (AGT→AGC) ytfMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (140/221);  total (140/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 377 total aligned reads displayed.

TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472
                                                                                           |                                                                                         
 gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCAtt                                                                                        <  2:5646327/93‑1 (MQ=255)
   tACACGCGGCACGCTGTGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCg                                                                                      <  1:4331884/93‑1 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCgg                                                                                     >  2:5512187/1‑92 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGt                                                                                     <  1:6667844/93‑1 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGt                                                                                     <  2:8487136/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGGCGACCGCAGCATTCGTgg                                                                                   <  2:9198941/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:4114942/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  1:9164146/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:5157814/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:9356366/93‑1 (MQ=255)
        gcgGGACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCt                                                                                 <  1:688465/93‑1 (MQ=255)
        gcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCt                                                                                 <  1:2897100/93‑1 (MQ=255)
         cgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCta                                                                                <  1:2699649/93‑1 (MQ=255)
          ggCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtac                                                                               <  1:6188253/93‑1 (MQ=255)
           gCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtaca                                                                              <  1:4589766/93‑1 (MQ=255)
           gCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtaca                                                                              <  2:7286302/93‑1 (MQ=255)
            cACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaa                                                                             <  2:2204573/93‑1 (MQ=255)
             aCGCTGGGCTGGATTGAACCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaa                                                                            <  1:3961909/93‑1 (MQ=255)
              cGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaat                                                                           <  1:7436007/93‑1 (MQ=255)
                 tgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaataca                                                                        <  2:1506520/93‑1 (MQ=255)
                 tgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGGGGCtccaaataca                                                                        >  2:8727802/1‑93 (MQ=255)
                  gggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaatacaa                                                                       >  2:2444795/1‑93 (MQ=255)
                  gggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaatacaa                                                                       >  2:3013016/1‑93 (MQ=255)
                  gggctcgATTTAAACCGGTGATTTCGACATAGAACGGCCGGATCTGCGATTGCTCCCC‑GGGGGCGACCGCAGCACTGGCGGCtacaaatacaa                                                                       >  2:7352741/1‑93 (MQ=255)
                   ggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaatacaaa                                                                      <  2:6856590/93‑1 (MQ=255)
                    gctggATTGAAACCGGTGATTTCGACAAAGTACCGCCTGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTacaaatacaaat                                                                     >  2:7904907/1‑93 (MQ=255)
                        gATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTAt                                                                 <  1:1382669/93‑1 (MQ=255)
                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATCCAAATCTATc                                                                <  2:80064/93‑1 (MQ=255)
                          ttGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCg                                                               >  2:2900230/1‑93 (MQ=255)
                          ttGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCg                                                               >  1:5998079/1‑93 (MQ=255)
                           tGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGc                                                              <  1:1117466/93‑1 (MQ=255)
                           tGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGc                                                              <  2:8260971/93‑1 (MQ=255)
                             aaaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTc                                                            <  1:943039/93‑1 (MQ=255)
                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACACATCTATCGCTcc                                                           >  2:5215600/1‑93 (MQ=255)
                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           <  2:3776313/93‑1 (MQ=255)
                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     <  1:5117345/93‑1 (MQ=255)
                                     gATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAc                                                    <  1:7087890/93‑1 (MQ=255)
                                     gATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAc                                                    >  2:2153609/1‑93 (MQ=255)
                                      aTTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACg                                                   <  2:3852575/93‑1 (MQ=255)
                                      aTTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACg                                                   >  2:6530115/1‑93 (MQ=255)
                                        ttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGcc                                                 <  2:8665450/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  2:7773027/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  1:4801607/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  2:5279865/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:3999477/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:1294614/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:7192787/93‑1 (MQ=255)
                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            <  1:8915731/93‑1 (MQ=255)
                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            >  2:2001923/1‑93 (MQ=255)
                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           >  2:7001985/1‑93 (MQ=255)
                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           <  1:6138831/93‑1 (MQ=255)
                                               aaGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCGACGGTg                                          >  2:7519137/1‑93 (MQ=255)
                                                aGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGa                                         <  1:4142176/93‑1 (MQ=255)
                                                 gTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAc                                        <  2:6716719/93‑1 (MQ=255)
                                                  tACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAcc                                       >  1:6780033/1‑93 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  <  1:8202724/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  <  2:4846346/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  <  2:4352662/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  >  1:5621848/1‑93 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  >  2:10001337/1‑93 (MQ=255)
                                                        cgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                 <  1:728563/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  2:1596019/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                >  2:3650880/1‑93 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               <  2:4758976/93‑1 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  2:1751827/1‑93 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  2:7120992/1‑93 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  1:4560596/1‑93 (MQ=255)
                                                           aTCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgggg                              >  1:4698627/1‑93 (MQ=255)
                                                            tCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                             >  2:2579392/1‑93 (MQ=255)
                                                            acTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                             <  2:6275244/92‑1 (MQ=255)
                                                              tGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                           >  1:2831323/1‑93 (MQ=255)
                                                              tGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                           <  1:1451394/93‑1 (MQ=255)
                                                               tcGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                          <  2:6939621/92‑1 (MQ=255)
                                                               gCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                          <  1:7680536/93‑1 (MQ=255)
                                                                cGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCg                         <  1:6401242/93‑1 (MQ=255)
                                                                  tttCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                       >  2:277259/1‑93 (MQ=255)
                                                                   ttcttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAg                      >  1:5194546/1‑93 (MQ=255)
                                                                   ttcttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAg                      <  1:2436445/93‑1 (MQ=255)
                                                                    tcttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                     >  1:9867551/1‑93 (MQ=255)
                                                                      ttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTg                   >  1:10025514/1‑93 (MQ=255)
                                                                       tcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGa                  >  1:3412655/1‑93 (MQ=255)
                                                                        cGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGaa                 >  1:8541554/1‑92 (MQ=255)
                                                                         gCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATa                >  1:5828769/1‑93 (MQ=255)
                                                                          gc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  2:3250677/92‑1 (MQ=255)
                                                                          cc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAATGGGGCCTCGAAGTTGATaa               <  2:3557970/93‑1 (MQ=255)
                                                                          cc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  1:1299808/93‑1 (MQ=255)
                                                                          cc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  2:4994801/93‑1 (MQ=255)
                                                                           c‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  1:7020338/93‑1 (MQ=255)
                                                                           c‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  1:8137046/93‑1 (MQ=255)
                                                                              ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg            >  1:6363921/1‑93 (MQ=255)
                                                                                ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          <  2:3342539/93‑1 (MQ=255)
                                                                                ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          >  1:7523016/1‑93 (MQ=255)
                                                                                  cGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATc        <  1:3071048/93‑1 (MQ=255)
                                                                                    aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCgt      >  1:3204596/1‑92 (MQ=255)
                                                                                    aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  2:5835788/1‑93 (MQ=255)
                                                                                    aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      <  2:5349222/93‑1 (MQ=255)
                                                                                     ccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCt     >  1:3808702/1‑93 (MQ=255)
                                                                                       gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg   >  1:2045596/1‑93 (MQ=255)
                                                                                       gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg   >  2:9866583/1‑93 (MQ=255)
                                                                                        cAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGa  >  2:8799117/1‑93 (MQ=255)
                                                                                           |                                                                                         
TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑