Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,483,536 T→C intergenic (‑17/‑250) pepA ← / → yjgP aminopeptidase A/I/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,483,5360TC99.6% 1724.4 / ‑5.6 524intergenic (‑17/‑250)pepA/yjgPaminopeptidase A/I/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (292/229);  total (293/231)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 532 total aligned reads displayed.

GGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACG  >  NC_000913/4483446‑4483625
                                                                                          |                                                                                         
tgTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCt                                                                                         <  1:3387642/92‑1 (MQ=255)
ggTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCt                                                                                         <  1:609570/93‑1 (MQ=255)
    cGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTaaa                                                                                     <  2:7446247/93‑1 (MQ=255)
     gAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAg                                                                                    >  2:8670159/1‑93 (MQ=255)
      aaGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGa                                                                                   >  2:7158042/1‑93 (MQ=255)
      aaGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGa                                                                                   <  2:3580010/93‑1 (MQ=255)
      aaGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGa                                                                                   <  1:3851540/93‑1 (MQ=255)
       aGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGAc                                                                                  >  1:6243898/1‑93 (MQ=255)
       aGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGAc                                                                                  >  1:4192914/1‑93 (MQ=255)
        gACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACa                                                                                 >  1:4513620/1‑93 (MQ=255)
           gCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcg                                                                              <  1:8486072/93‑1 (MQ=255)
            cccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcgg                                                                             >  1:6629216/1‑93 (MQ=255)
             ccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggc                                                                            <  2:988452/93‑1 (MQ=255)
             ccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggc                                                                            >  2:5468343/1‑93 (MQ=255)
               acgacgATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcgg                                                                          <  1:964320/93‑1 (MQ=255)
                 gacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTg                                                                        <  1:7162084/93‑1 (MQ=255)
                    gaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCt                                                                     <  1:3485065/93‑1 (MQ=255)
                     aTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTa                                                                    >  1:8844244/1‑93 (MQ=255)
                       gCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACa                                                                  <  2:563051/93‑1 (MQ=255)
                        cAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAg                                                                 >  1:9414262/1‑93 (MQ=255)
                         aGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAGCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGa                                                                <  2:4168675/93‑1 (MQ=255)
                         aGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGa                                                                >  1:6395421/1‑93 (MQ=255)
                         aGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGa                                                                <  2:5823553/93‑1 (MQ=255)
                          ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               <  2:2206150/93‑1 (MQ=255)
                          ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               >  2:3787185/1‑93 (MQ=255)
                           gCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATa                                                              <  1:3583330/93‑1 (MQ=255)
                              cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           <  1:5230386/93‑1 (MQ=255)
                              cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           <  2:9202721/93‑1 (MQ=255)
                              cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           >  1:2781734/1‑93 (MQ=255)
                               tCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAAt                                                          >  1:6816505/1‑93 (MQ=255)
                               tCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAAt                                                          >  2:6529076/1‑93 (MQ=255)
                               tCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAAt                                                          >  2:4728335/1‑93 (MQ=255)
                               tCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAAt                                                          >  1:4434337/1‑93 (MQ=255)
                                    tGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGGaa                                                     >  2:497219/1‑93 (MQ=255)
                                     gTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAg                                                    >  1:2498279/1‑93 (MQ=255)
                                     gTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAg                                                    <  1:7690253/93‑1 (MQ=255)
                                      tttCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGc                                                   <  2:2374486/93‑1 (MQ=255)
                                        tctcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCtt                                                 >  1:5814821/1‑93 (MQ=255)
                                         ctcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCttt                                                <  2:4276448/93‑1 (MQ=255)
                                         ctcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCttt                                                <  2:3166679/93‑1 (MQ=255)
                                          tcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTc                                               >  2:3474252/1‑93 (MQ=255)
                                          cccGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTc                                               <  2:6859788/92‑1 (MQ=255)
                                               gCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAAc                                          <  2:6592026/93‑1 (MQ=255)
                                               gCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGAAAGCTTTCGTAAc                                          >  2:3057924/1‑93 (MQ=255)
                                                cTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACt                                         >  1:5745926/1‑93 (MQ=255)
                                                 tACCGCTTTTTACACTAAACTTCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTc                                        >  1:1720295/1‑93 (MQ=255)
                                                 tACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTc                                        >  2:8366906/1‑93 (MQ=255)
                                                 caCCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTc                                        <  2:2555067/92‑1 (MQ=255)
                                                  aCCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCa                                       >  2:4571746/1‑93 (MQ=255)
                                                   ccGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCAt                                      >  1:7335466/1‑93 (MQ=255)
                                                    cGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATg                                     >  1:5990275/1‑93 (MQ=255)
                                                     gCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGt                                    >  2:8223468/1‑93 (MQ=255)
                                                     gCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGt                                    <  2:280901/93‑1 (MQ=255)
                                                      cTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTc                                   >  2:9556116/1‑93 (MQ=255)
                                                         tttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAGCTCATGTCCGc                                >  1:7250375/1‑93 (MQ=255)
                                                         tttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGc                                <  2:10395/93‑1 (MQ=255)
                                                          ttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCt                               <  2:1297250/93‑1 (MQ=255)
                                                           tccacTAAACTCCATGCACTACGCTCATGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTg                              <  2:4807890/91‑1 (MQ=255)
                                                           tACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTg                              >  2:6992459/1‑93 (MQ=255)
                                                            atacTGAACTCCATGCACAGCGCTCCTGTGTATTAAAGACAACGGCGGTGGCTACAGATAGAATTACAAGCTTTCGTAACTCATGTCCGCTGt                             <  1:1582600/91‑1 (MQ=255)
                                                            acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             >  1:8074235/1‑93 (MQ=255)
                                                            acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             <  1:710987/93‑1 (MQ=255)
                                                               cTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGc                          <  2:777951/93‑1 (MQ=255)
                                                               cTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGc                          >  1:3800779/1‑93 (MQ=255)
                                                                tAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCg                         <  1:1985519/93‑1 (MQ=255)
                                                                 aaaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGa                        <  2:7634266/93‑1 (MQ=255)
                                                                 aaaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGa                        <  2:6028307/93‑1 (MQ=255)
                                                                  agcTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       <  2:913324/91‑1 (MQ=255)
                                                                  aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       >  1:9289799/1‑93 (MQ=255)
                                                                  aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       >  2:5257413/1‑93 (MQ=255)
                                                                  aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       <  2:8471185/93‑1 (MQ=255)
                                                                   aCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATg                      >  1:321548/1‑93 (MQ=255)
                                                                    cTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGa                     <  1:7483875/93‑1 (MQ=255)
                                                                     tCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTTCGATGAc                    >  2:6328718/1‑93 (MQ=255)
                                                                     tCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGAc                    >  1:4698290/1‑93 (MQ=255)
                                                                       cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  >  1:5317159/1‑93 (MQ=255)
                                                                       cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  >  2:5092446/1‑93 (MQ=255)
                                                                       cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  >  1:8610963/1‑93 (MQ=255)
                                                                       cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTACAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  >  1:8281801/1‑93 (MQ=255)
                                                                         tGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCg                >  1:10026359/1‑93 (MQ=255)
                                                                         tGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCg                <  2:7900320/93‑1 (MQ=255)
                                                                         tGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCg                >  1:1304249/1‑93 (MQ=255)
                                                                           cACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGTCTTCgtg              >  2:1499188/1‑93 (MQ=255)
                                                                           cACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtg              <  1:2863706/93‑1 (MQ=255)
                                                                            aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             >  2:9769717/1‑93 (MQ=255)
                                                                            aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             <  2:9016851/93‑1 (MQ=255)
                                                                            aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             >  2:1840512/1‑93 (MQ=255)
                                                                              tACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTa           >  1:5607130/1‑93 (MQ=255)
                                                                              tACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTa           >  1:7968527/1‑93 (MQ=255)
                                                                              tACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTa           >  1:2326036/1‑93 (MQ=255)
                                                                               ccGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGCAACTCATGTCCGCCGTTGCGATGACTTCGTGTTaa          <  1:1847310/92‑1 (MQ=255)
                                                                               aCGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTaa          <  1:6296546/93‑1 (MQ=255)
                                                                                cGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAAt         >  2:1647976/1‑93 (MQ=255)
                                                                                 gCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATc        >  1:2574157/1‑93 (MQ=255)
                                                                                  caccTGAGCCTTAAAGACAACGGCGGTGGCTACAGATAGAAGTTCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCt       <  1:1216868/91‑1 (MQ=255)
                                                                                  cTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCt       >  1:9123399/1‑93 (MQ=255)
                                                                                   tCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCtt      >  1:4909864/1‑93 (MQ=255)
                                                                                   tCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCtt      >  1:9887257/1‑93 (MQ=255)
                                                                                     cTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTaa    >  2:809155/1‑93 (MQ=255)
                                                                                       gAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACg  >  2:391556/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACG  >  NC_000913/4483446‑4483625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑