BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,484,843	T→C	V353A (GTC→GCC)	yjgP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,484,843	0	T	C	99.8%	1408.9 / ‑5.2	441	V353A (GTC→GCC)	yjgP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (215/224); total (216/225)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 468 total aligned reads displayed.

GTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGTCCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTT  >  NC_000913/4484753‑4484932

 tAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccg                                                                                        >  1:4797/1‑93 (MQ=255)

     cTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccgccac                                                                                    <  1:5440767/93‑3 (MQ=255)

        gACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTg                                                                                 <  1:7637079/93‑1 (MQ=255)

        gACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTg                                                                                 <  1:5702668/93‑1 (MQ=255)

        gACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTg                                                                                 <  2:5637827/93‑1 (MQ=255)

         aCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTgc                                                                                >  1:3866425/1‑93 (MQ=255)

          cccGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTgcg                                                                               <  1:1460812/93‑1 (MQ=255)

              aCGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGcc                                                                           <  1:4057827/93‑1 (MQ=255)

               cGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCa                                                                          >  1:2826230/1‑93 (MQ=255)

               cGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCa                                                                          <  2:6215655/93‑1 (MQ=255)

                gCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAg                                                                         >  2:7620010/1‑93 (MQ=255)

                   gtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttt                                                                      >  1:5520441/1‑93 (MQ=255)

                    tggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGtttt                                                                     <  1:2613310/93‑1 (MQ=255)

                     ggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttttt                                                                    <  2:3895683/93‑1 (MQ=255)

                      gatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTc                                                                   >  1:5894304/1‑93 (MQ=255)

                        tgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTcgc                                                                 <  2:177208/93‑1 (MQ=255)

                            gaCCGTTAACGTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTaa                                                             <  1:9749855/93‑1 (MQ=255)

                            gaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTaa                                                             <  2:5926655/93‑1 (MQ=255)

                             aCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTaaa                                                            <  2:9179232/93‑1 (MQ=255)

                             aCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTaaa                                                            <  2:5273141/93‑1 (MQ=255)

                              ccGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAg                                                           <  1:5069422/93‑1 (MQ=255)

                               cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAgg                                                          >  2:1260952/1‑93 (MQ=255)

                               cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAgg                                                          >  1:7785395/1‑93 (MQ=255)

                                gTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                         >  1:8404023/1‑93 (MQ=255)

                                gTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                         >  1:251673/1‑93 (MQ=255)

                                gTTAACCTGATTTATCTGGCTTTAGCGATGGTTCTCAGCCTTTGGGCCACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                         <  2:308117/93‑1 (MQ=255)

                                 ttAACCTTATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTCCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAg                                                        <  2:5190002/93‑1 (MQ=255)

                                 ttAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAg                                                        <  1:2287371/93‑1 (MQ=255)

                                 ttAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAg                                                        >  2:6426916/1‑93 (MQ=255)

                                  tAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGc                                                       <  2:8293871/93‑1 (MQ=255)

                                    aCCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCgg                                                     >  1:1932753/1‑93 (MQ=255)

                                    aCCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCACGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCgg                                                     >  1:1240148/1‑93 (MQ=255)

                                     ccTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgt                                                    <  1:8614176/93‑1 (MQ=255)

                                      cTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtg                                                   >  1:1029874/1‑93 (MQ=255)

                                       tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgt                                                  <  1:9031256/93‑1 (MQ=255)

                                        gATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGtgtg                                                 >  1:6935521/1‑93 (MQ=255)

                                        gATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGggtg                                                 >  2:2144363/1‑93 (MQ=255)

                                         aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                <  1:7151717/93‑1 (MQ=255)

                                         aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                >  1:3453792/1‑93 (MQ=255)

                                         aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                <  2:4424845/93‑1 (MQ=255)

                                           ttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATg                                              >  1:10041900/1‑93 (MQ=255)

                                            tATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGc                                             >  1:1369166/1‑93 (MQ=255)

                                                tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                         >  1:5307828/1‑93 (MQ=255)

                                                 ggCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCt                                        >  2:1405694/1‑93 (MQ=255)

                                                  gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                       <  2:4722524/93‑1 (MQ=255)

                                                  gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                       >  1:3336045/1‑93 (MQ=255)

                                                  gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                       <  1:497202/93‑1 (MQ=255)

                                                    tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                     <  2:8773891/93‑1 (MQ=255)

                                                    tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                     >  2:7147173/1‑93 (MQ=255)

                                                    tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                     <  2:3600324/93‑1 (MQ=255)

                                                    tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                     >  2:7281440/1‑93 (MQ=255)

                                                    tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCACGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                     <  1:3188539/93‑1 (MQ=255)

                                                     ttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTg                                    >  1:6612835/1‑93 (MQ=255)

                                                     ttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTg                                    <  2:8690030/93‑1 (MQ=255)

                                                     ttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCCGCCCGCCACCTGCGCGCCAGCTTTTCGCGTAAAGCAGCGCGGTGATGCAACCTTTTg                                    >  2:7536582/1‑93 (MQ=255)

                                                        gCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCg                                 <  2:7821942/93‑1 (MQ=255)

                                                        gCGATTGTTCTCAACCTTTGGGACACCGCGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCg                                 >  2:7980069/1‑93 (MQ=255)

                                                         cGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGt                                <  2:1812584/93‑1 (MQ=255)

                                                         cGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGt                                >  1:1718516/1‑93 (MQ=255)

                                                         cGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGt                                <  2:5011503/93‑1 (MQ=255)

                                                          gATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTa                               <  1:9214790/93‑1 (MQ=255)

                                                          gATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTa                               >  1:6363711/1‑93 (MQ=255)

                                                               ttCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGa                          >  2:4136611/1‑93 (MQ=255)

                                                                 ctcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAcc                        <  2:9457284/93‑1 (MQ=255)

                                                                 ctcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAcc                        >  2:2452266/1‑93 (MQ=255)

                                                                  tcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCg                       <  1:9532538/93‑1 (MQ=255)

                                                                  tcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCg                       >  1:8888846/1‑93 (MQ=255)

                                                                   cAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGc                      >  2:2572987/1‑93 (MQ=255)

                                                                   cAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGc                      <  1:8054698/93‑1 (MQ=255)

                                                                   cAACCTTTGGCACACCGTGCCGGCCCGACACCTGCGCGCCAGTTTTTCCCGTGAAGGAGCGGAGTGATGCAACCTTTTGGCGTATTTGCCCGc                      >  2:1535416/1‑93 (MQ=255)

                                                                    aaCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCt                     >  1:2666278/1‑93 (MQ=255)

                                                                     aCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCta                    >  2:6789780/1‑93 (MQ=255)

                                                                     aCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCta                    >  2:5475226/1‑93 (MQ=255)

                                                                     aCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCta                    >  1:7380509/1‑93 (MQ=255)

                                                                     aCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCta                    >  1:6784530/1‑93 (MQ=255)

                                                                     aCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCta                    <  2:3496079/93‑1 (MQ=255)

                                                                        tttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTatat                 >  2:1135318/1‑93 (MQ=255)

                                                                        tttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTatat                 <  2:5085447/93‑1 (MQ=255)

                                                                         ttGGGACACCGTGCCGGCCCGCCACCGGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATc                <  2:6164423/93‑1 (MQ=255)

                                                                            ggACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTCCTTGACCGCTATACCgga             >  1:459669/1‑92 (MQ=255)

                                                                              acacCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaa           >  2:4477203/1‑93 (MQ=255)

                                                                               cacCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaa          >  1:5552124/1‑93 (MQ=255)

                                                                               cacCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaa          <  2:3947929/93‑1 (MQ=255)

                                                                                acCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaaa         >  2:7873930/1‑93 (MQ=255)

                                                                                 ccGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAc        <  1:6192431/93‑1 (MQ=255)

                                                                                 ccGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAc        >  2:2341409/1‑93 (MQ=255)

                                                                                   gTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTa      >  2:6054234/1‑93 (MQ=255)

                                                                                    tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt     >  2:3606510/1‑93 (MQ=255)

                                                                                    tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt     >  1:3027894/1‑93 (MQ=255)

                                                                                    tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt     >  2:5565101/1‑93 (MQ=255)

                                                                                    tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt     >  1:7491662/1‑93 (MQ=255)

                                                                                     gCCGGCGCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtt    <  2:8879368/93‑1 (MQ=255)

                                                                                     gCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtt    <  2:5384105/93‑1 (MQ=255)

                                                                                     gCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtt    <  2:10005308/93‑1 (MQ=255)

                                                                                     gCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtt    <  2:2828970/93‑1 (MQ=255)

                                                                                     gCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtt    <  2:4012415/93‑1 (MQ=255)

                                                                                      ccGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAttt   <  2:969241/93‑1 (MQ=255)

                                                                                      ccGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAATAttt   >  2:9775409/1‑93 (MQ=255)

                                                                                       cGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtttt  <  1:8478594/93‑1 (MQ=255)

                                                                                       cGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAtttt  <  1:4385604/93‑1 (MQ=255)

GTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGTCCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTT  >  NC_000913/4484753‑4484932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`