BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,106	A→G	E408G (GAG→GGG)	nadR →	NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,106	0	A	G	89.6%	922.9 / 79.7	368	E408G (GAG→GGG)	nadR	NadR
Reads supporting (aligned to +/- strand): ref base A (11/27); new base G (154/174); total (166/202)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 389 total aligned reads displayed.

AGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGA  >  NC_000913/4626014‑4626198

   gaACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGcg                                                                                           >  2:2474414/1‑92 (MQ=255)

    aaCAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCag                                                                                          >  1:159460/1‑93 (MQ=255)

     aCAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCaga                                                                                         <  2:2588513/93‑1 (MQ=255)

      cAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCagag                                                                                        >  2:2262859/1‑93 (MQ=255)

      cAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCagag                                                                                        <  1:8353053/93‑1 (MQ=255)

       aaTATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAgaga                                                                                       >  1:6847719/1‑93 (MQ=255)

        atatCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGAt                                                                                      <  2:8377525/93‑1 (MQ=255)

         tatCGAATTTGTGCGGGTTGAAGAGGAAGATTGCGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGGGGCAGAGAt                                                                                      <  1:1881628/93‑1 (MQ=255)

          atCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATaa                                                                                    >  2:7132495/1‑93 (MQ=255)

          atCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATaa                                                                                    >  1:2547088/1‑93 (MQ=255)

          atCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATaa                                                                                    <  1:177573/93‑1 (MQ=255)

          atCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGACATGAT‑GGGGGGGCAGAGATaa                                                                                    <  2:6445833/93‑1 (MQ=255)

           tCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAAc                                                                                   >  2:7849855/1‑93 (MQ=255)

            cGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAAcg                                                                                  >  1:3985140/1‑92 (MQ=255)

            cGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAAcc                                                                                  <  2:3612761/93‑1 (MQ=255)

               aTTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGtg                                                                               >  1:572325/1‑93 (MQ=255)

                tttGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGtga                                                                              <  2:2894705/93‑1 (MQ=255)

                      cGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAAc                                                                        <  1:8024553/93‑1 (MQ=255)

                       gggTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACt                                                                       <  2:4776579/93‑1 (MQ=255)

                           tGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTc                                                                   >  2:8305932/1‑93 (MQ=255)

                            gAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCa                                                                  <  2:7244191/93‑1 (MQ=255)

                            gAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCa                                                                  <  1:702022/93‑1 (MQ=255)

                            gAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGCGATGAAACTGCTCa                                                                  <  1:7123412/93‑1 (MQ=255)

                             aaGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCaa                                                                 <  1:1455641/93‑1 (MQ=255)

                             aaGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCaa                                                                 >  2:2190323/1‑93 (MQ=255)

                               gagGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAg                                                               >  2:1811145/1‑93 (MQ=255)

                                  gAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCg                                                            <  2:4278313/93‑1 (MQ=255)

                                    aGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAg                                                          >  2:4064111/1‑93 (MQ=255)

                                     gATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAgg                                                         <  1:913454/93‑1 (MQ=255)

                                     gATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAgg                                                         >  1:330270/1‑93 (MQ=255)

                                      aTTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGt                                                        <  1:6467580/93‑1 (MQ=255)

                                      aTTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGt                                                        >  2:9891786/1‑93 (MQ=255)

                                       ttACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGta                                                       <  1:5439569/93‑1 (MQ=255)

                                       ttACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGta                                                       <  2:542845/93‑1 (MQ=255)

                                          cGACAGTCGTTTCGTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAAATAACCGTGATGAAACTGCTCAAAGGCGAGGTATaa                                                    <  2:1101650/93‑1 (MQ=255)

                                          cGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATaa                                                    <  1:4802341/93‑1 (MQ=255)

                                           gACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATaaa                                                   >  1:9391260/1‑93 (MQ=255)

                                           caCAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATaaa                                                   <  1:298885/92‑1 (MQ=255)

                                            aCAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGG‑GCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAAt                                                 <  1:8500127/93‑1 (MQ=255)

                                             cAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAAt                                                 <  1:4513878/93‑1 (MQ=255)

                                              aGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACAGCTCAAAGGCGAGGTATAAAATg                                                >  2:5532892/1‑93 (MQ=255)

                                               gTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATGACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGa                                               >  1:510598/1‑93 (MQ=255)

                                               gTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGa                                               >  1:8698786/1‑93 (MQ=255)

                                               gTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGa                                               >  1:9733520/1‑93 (MQ=255)

                                                 cGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGt                                             >  1:9226419/1‑93 (MQ=255)

                                                   tttCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGttt                                           <  2:6325834/93‑1 (MQ=255)

                                                   tttCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGttt                                           >  1:2180424/1‑93 (MQ=255)

                                                    ttCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGtttt                                          >  1:1055865/1‑93 (MQ=255)

                                                      ccTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGtttttt                                        <  1:7255212/93‑1 (MQ=255)

                                                      ccTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGtttttt                                        >  1:2351646/1‑93 (MQ=255)

                                                       ctgcgctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGttttttt                                       >  2:7367010/1‑93 (MQ=255)

                                                        tgcgctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTtg                                      >  2:1017329/1‑93 (MQ=255)

                                                        tgcgctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTtg                                      >  2:4670990/1‑93 (MQ=255)

                                                        tgcgctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGGATAAAATGAGTTTTTTtg                                      >  2:9171342/1‑93 (MQ=255)

                                                        tgcgctgcgTGGAACTGGGGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTtg                                      <  1:7598774/93‑1 (MQ=255)

                                                           gctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTtgatg                                   <  2:8829633/93‑1 (MQ=255)

                                                           gctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTtgatg                                   <  1:8253570/93‑1 (MQ=255)

                                                            ctgcgTGGAACTGGTGCGGGAGGTGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGAGAGGTATAAAATGAGTTTTTTtgatga                                  >  1:6941345/1‑93 (MQ=255)

                                                            ctgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTtgatga                                  >  2:6006399/1‑93 (MQ=255)

                                                             tgcgTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAg                                 >  1:2665593/1‑93 (MQ=255)

                                                              gcgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGt                                <  1:1266316/93‑1 (MQ=255)

                                                               cgTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGtt                               <  2:9354605/93‑1 (MQ=255)

                                                                gTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGtta                              >  1:3386427/1‑92 (MQ=255)

                                                                gTGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTg                              >  2:7501152/1‑93 (MQ=255)

                                                                 tGGAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGa                             >  1:1204900/1‑93 (MQ=255)

                                                                  ggAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAGCTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGaa                            <  1:4360737/93‑1 (MQ=255)

                                                                  ggAACTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGaa                            >  2:7434815/1‑93 (MQ=255)

                                                                  ggAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGaa                            <  1:4394749/93‑1 (MQ=255)

                                                                  ggAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGaa                            <  1:4628204/93‑1 (MQ=255)

                                                                    aaCTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGaaaa                          <  1:3641763/93‑1 (MQ=255)

                                                                    aaCTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGaaaa                          <  1:8996574/93‑1 (MQ=255)

                                                                     aCTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAAc                         <  2:4445152/93‑1 (MQ=255)

                                                                     aCTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAAc                         <  2:4835228/93‑1 (MQ=255)

                                                                     aCTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAAc                         <  1:5736593/93‑1 (MQ=255)

                                                                      cTGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAAcc                        >  2:6767420/1‑93 (MQ=255)

                                                                       tGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACct                       <  1:4990266/93‑1 (MQ=255)

                                                                       tGGTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACct                       >  2:1941488/1‑93 (MQ=255)

                                                                        ggTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACctc                      >  1:7318535/1‑93 (MQ=255)

                                                                        ggTGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACctc                      >  2:3298873/1‑93 (MQ=255)

                                                                          tGCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACctctc                    <  2:9673625/93‑1 (MQ=255)

                                                                           gCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACctctct                   <  2:2722497/93‑1 (MQ=255)

                                                                           gCGGGAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACctctct                   <  2:7781201/93‑1 (MQ=255)

                                                                             gggAGATGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTgg                 >  2:8723730/1‑93 (MQ=255)

                                                                               gagaTGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGaa               <  1:7779265/93‑1 (MQ=255)

                                                                               gagaTGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGaa               <  2:8037728/93‑1 (MQ=255)

                                                                                agaTGAT‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAag              <  2:5323983/93‑1 (MQ=255)

                                                                                 gatgat‑GGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAaga             <  2:2375027/93‑1 (MQ=255)

                                                                                  atgat‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAagag            <  2:3531902/93‑1 (MQ=255)

                                                                                  atgat‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAagag            >  1:5557283/1‑93 (MQ=255)

                                                                                   tgat‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAgg           >  2:1593521/1‑93 (MQ=255)

                                                                                   tgat‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAgg           >  1:6131891/1‑93 (MQ=255)

                                                                                   tgat‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAgg           >  2:6164137/1‑93 (MQ=255)

                                                                                    gat‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGc          <  2:7939335/93‑1 (MQ=255)

                                                                                     at‑gGGGGGGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCt         >  1:206245/1‑93 (MQ=255)

                                                                                        ggggggGCAGAGATAACCGTGATGAAACTGCTCCAAGGAGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGt       <  2:8956098/93‑1 (MQ=255)

                                                                                        ggggggGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGt       <  2:9436456/93‑1 (MQ=255)

                                                                                        ggggggGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGt       <  1:3906648/93‑1 (MQ=255)

                                                                                         ggggggCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTc      <  2:3128398/93‑1 (MQ=255)

                                                                                         ggggggCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTc      <  2:3762312/93‑1 (MQ=255)

                                                                                           ggAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCga    <  2:8561825/93‑1 (MQ=255)

AGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGAT‑GGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGA  >  NC_000913/4626014‑4626198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`